JPS62102156A - Agglomeration reaction chamber for liquid particle reagent - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION (Industrial Field of Application) The present invention generally relates to immunochemical particle reagents (immunochemical particle reagents).
chemical parti-cle rea
In more detail, an agglutination reaction chamber (aggIutinographic
re-action chamber). Stable and without the need for external movement
In this specification, a method for producing a well-contrasted tail and a recognizable immunochemical particle aggregation reaction is described as "agglutinography".
agglut +nographv)J or "aggregation reaction".

(Prior Art and Problems to be Solved by the Invention) Methods of reacting immunochemical particle reagents using laboratory slides are well known, but these known methods do not give satisfactory results. , usually by placing a liquid reagent on a glass slide and gently rocking the slide.
6. If the particle reagent is not rotated, there will be no visible aggregation. cannot be successfully formed.

In many cases, for optimal performance, the rocking and turning of the slide must be done precisely and over a precise amount of time; Such known methods, which must be read immediately, yield inaccurate results under some conditions. If the rotation is too fast or too slow, or if the agitation and rotation are too short or too long, the reagent will not react completely or will react too much, resulting in a zero reaction within the agitation time. If no reaction is observed at the end, one reaction will be continued.

Conventional agglutination reagents also evaporate in a short period of time, so any visible record of a particular reaction quickly disappears, and the reagents spill over the edges of the slide during agitation or other processing. Naturally, the results will be inaccurate.

(Means for Solving the Problems) According to the present invention, the zero reaction chamber in which the aggregation reaction chamber for liquid particle reagents is provided overlaps the first panel and at least a part of this first panel. It consists of a second panel. The non-polymerized area of the first panel forms the containment area. The first panel is spaced a predetermined distance from the second panel and defines a chamber between the first panel and the second panel.

Upon introduction of the liquid particle reagent into or near the containment area, capillary forces move the liquid reagent into the chamber and a flocculation reaction occurs between the first panel and the second panel.

An object of the present invention is to provide an aggregation reaction chamber.

Another object of the present invention is to provide an agglutination reaction chamber in which agglutination can be performed without agitation or rotation.

Another object of the present invention is to provide an agglutination reaction chamber that is capable of forming reproducible and easily observed agglomerates without requiring time for one reaction.

A further object of the present invention is to provide a flocculation reaction chamber in which a record of the flocculation reaction can be obtained.A further object of the present invention is to provide a flocculation reaction chamber that is simple to use and allows reproducible flocculation reactions to be carried out. The purpose is to provide a reaction chamber.

Other objects and advantages of the invention will become apparent from the description below.

EXAMPLE Referring first to FIGS. 1A to 1C, there is shown a flocculation reaction chamber, generally designated by the reference numeral IO, constructed in accordance with the present invention. The aggregation reaction chamber 10 includes an upper panel 20 made of glass and a lower panel 2 made of glass.
A narrow space DD is defined between the panels 20 and 21, consisting of 1 and connected by a spacer 30, to provide a flat capillary chamber 15. A storage area 22 is formed by the area where the smaller panel 20 does not overlap the panel 21. Immunochemical particle reagent in liquid 23 is supplied to containment area 22 . Reagent 12 has 1 capillary chamber 15
is placed near the end 26 of.

The immunoparticle reagent 23 is then drawn into the capillary chamber 15 by capillary action and spreads throughout the chamber. Panels 20 and 21
may have the same size as reagent 1, in which case reagent 2
3 is introduced into the capillary chamber 15 at the inlet.

In order to understand the manner in which the agglutination reaction test occurs using the agglutination reaction chamber of the present invention, the agglutination reaction will be explained using the first basic steps. The antibody reagent and polystyrene latex reagent, which are test samples, are placed in the storage area 22 of the slide 10 (7).
Placed with a pipette.

Reagents and test sample 23 are drawn into capillary chamber 15 . When the molecule of interest is absent from the test sample, the reagent sample begins to react and form small aggregates.

This capillary flow inherent in the aggregation reaction chamber provides the necessary driving force for the formation of aggregates. When two particles react to form an aggregate, their rate of association becomes smaller relative to unreacted particles.

This decrease in velocity is expressed by Stokes' law as follows:

According to Stokes' law, for a particle in a liquid of viscosity n, the velocity of the particle relative to the velocity of the liquid is V.

It is inversely proportional to the radius r of the particle.

Agglomerates of particles therefore move more slowly than non-agglomerated particles, similarly large agglomerates of one particle move more slowly than small agglomerates of particles, and furthermore, the molecules in question is present, the agglutination reaction will react in proportion to the amount of molecules of interest present.

When explaining Diagrams 1A to IC, as the liquid reagent moves from right to left, the unreacted particles behind the aggregates will experience a relative speed difference between the unreacted particles and the aggregated particles. Such collisions, which impinge on the agglomerates, result in even slower moving larger agglomerates, thus resulting in greater velocity of collisions with unreacted particles and smaller agglomerates.

This reaction continues until the size of the aggregates increases to such an extent that the velocity of the aggregates reaches zero, at which point unreacted particles either attach to or surround the aggregated particles. Continue passing through the aggregate in liquid flux. This results in a significantly high contrast visual representation of the aggregates when they occur in the test sample.

Next, referring to FIGS. 2 to 2CII and FIGS. 3A to 30, the aggregation reaction occurring in the aggregation reaction chamber of FIG. 1 is continuously shown. Figures 2A and 3A are
The agglutination reagent 23 is stored in the storage area 2 near the portion 26 of the capillary chamber 15.
2 shows the state of the reaction when placed with a pipette. Reagent 22 is drawn into capillary chamber 15 by capillary action. Liquid reagent 23 is continuously drawn into capillary chamber 15 in the direction of arrow 27 until the distal end of capillary chamber 15 is reached.

Referring now to Figures 2B and 3B, liquid reagent 23 has entered capillary chamber 15 and begun to aggregate to form small aggregates 26. When liquid reagent 23 is drawn into capillary chamber 15, a liquid flow occurs in the direction of arrow 27 and the agglomerated particles within reagent 23 begin to agglomerate to form small aggregates.

Referring now to Figures 2C and 30, these figures show a completed agglutination reaction. The reaction is 1
It ends when capillary forces are gone, the reagents are flowing, and the agglutination chamber is visually stable.

As shown more clearly in Figure 3C, the agglomerates are so highly formed that they can be seen uniformly over the entire area of the capillary chamber.

Since the flocculation reaction is maintained between the two glass panels 20 and 21, the liquid does not evaporate quickly;
The completed reaction is in a stable state. As a result, the completed reaction can be observed for a much longer period of time than in the case of prior art flocculation reactions exposed to ambient atmosphere.In the preferred embodiment of the invention shown in FIG. 10 is configured as follows. The lower panel 21 and the lower panel 20 are made of glass. The lower panel 21 is, for example, 50 mm x 50 mm,
It is a glass slide with a thickness of 1 mm, and the top panel is
It is a glass slide measuring 50 mm x 40 mm and having a thickness of inm. These two glass panels are spaced apart by a distance of approximately 3 microns and define a capillary chamber 15. The reagent 23 is coated with HCG and has a diameter of 0.
It consists of a mixture of 3 mm polystyrene latex and an aggregation reagent such as a solution of an antibody against HCG.

Of course, panels 20 and 21 need not be of such dimensions or made of glass. However, at least one of the upper panel 20 and the lower panel 21 must be provided with a wettable surface forming the surface of the capillary chamber 15.

The distance D-D between the h section sheet 20 and the lower sheet 21 is about 0.1 to 500 microns, preferably 2 to 20 microns.
It can range from 3 to 7 microns, more preferably from 3 to 7 microns. The optimal distance D-D varies depending on the size of the unreacted particles. The larger the unreacted particles, the larger the distance D-D must be to produce an optimal coagulation-assembly reaction; similarly, the smaller the unreacted particles, the smaller the distance D-D. capillary chamber 1
5, the reaction occurs more efficiently.

Distance D-D can be determined by a number of different types to maintain a spaced relationship between sheets 20 and 21, which is maintained by spacer 30 between h-section panel 20 and bottom panel 21. Spacers can be used. The spacer 30 is made of paint, screen printing (s
flk-screening), ink, polyester film, irregular or discrete film on the surface of the slide. Depending on the distance D-D to be used in the special agglutination chamber, the reagents to be used in the agglutination chamber, and manufacturing tolerances, various materials can be used to obtain optimal results.

Referring now to FIG. 4, there is shown another embodiment of an aggregation reaction chamber, generally designated by the reference numeral 35, constructed in accordance with the present invention. The aggregation reaction chamber 35 consists of an upper glass panel 40 and a lower glass panel 41 separated by a distance D-D. The embodiment of FIG. 4 is similar to the first
The configuration is similar to the embodiments shown in FIGS. Increasing the length in this way increases the path for the liquid to travel and increases the time for the active reaction. Using such long slides allows the aggregates to be more widely distributed.

Referring now to FIG. 5, there is shown a flocculation reaction chamber 45 constructed in accordance with another embodiment of the present invention. It consists of three upper panels 47a, 47b, and 47c with different values. The embodiment of FIG. 5 is similar in other functional features to the embodiment shown in FIG. By using three top panels of different lengths, aggregation can be displayed to different extents with the same reagent.

Although three top panels are used in the embodiment of FIG. 5, two top panels or more than four north panels can be used depending on the requirements.

Next, FIGS. 6A and 6B will be explained.

A flocculation reaction chamber, generally designated by the reference numeral 55, constructed in accordance with another embodiment of the present invention is shown. In this embodiment, the top panel 50 has a circular opening 5 in the center.
It has 2. J-, part panel 50 and lower panel 51
The outer dimensions of are the same.

In this example, reagents are pipetted into the lower panel 51 through the apertures 52. Such a shape provides a radial pattern of aggregates and eliminates the possibility of reagent spillage. This example is similar to the first example except that a reagent storage area is provided.
It has the same functional and structural features as described above with respect to the figures. Furthermore, this embodiment provides a radial distribution pattern.

Referring now to FIG. 7, FIG.
0 reaction chamber 65 where the flocculation reaction chamber is shown.
comprises a lower panel 61 and an upper panel 60a, both of which function and have similar features of construction and similar advantages to those of the embodiment described with respect to FIG. However, capillary chamber 65 also has a second top panel that overlaps panel 60a and has the same length as panel 60a. With such a configuration, the two layers of aggregates visually overlap, making it possible to easily and reliably observe the agglutination reaction. Spacer 63 is disposed between panel 60a and panel 6ob, and spacer 64 is disposed between panel 60a and panel 60 to maintain these three panels in a spaced relationship.
Spacers 63 and 64 can be adjusted to form two capillary chambers of similar or different dimensions.

Referring now to FIGS. 8A and 8B, af& is shown in accordance with another embodiment of the present invention, and is generally designated by the reference numeral 7.
A flocculation reaction chamber designated by 5 is shown. P.J.S.
The embodiment of Figures 8A and 8B has a similar construction to the embodiment of Figure 1, and like parts have been given the same reference numerals. One or more bands of chemicals are disposed between panels 20 and 21 within the passageway of the liquid reagent in capillary chamber 15 to provide another desired effect. The desired effect may be chemical or visual.

Place a certain reagent in the agglutination reaction chamber 1 Opening 2 of capillary chamber 15
It may be desirable to introduce another reagent at step 6. This way, you can eliminate the need to arm the reagents before introducing them into the reaction chamber. Make sure that the liquid placed at the edge of the capillary surface will clump due to the presence of another chemical. To do.

Several different chemicals can be used.

(Effects) As described above, according to the present invention, the reaction automatically proceeds to the end without the need for shaking or rotation, and it is possible to prevent the reagent from spilling, and to prevent the reaction from spilling. It is possible to obtain a flocculation reaction chamber that is visually stable for a long time without requiring monitoring and without causing actual evaporation.

The above objects of the present invention are effectively achieved.

1. It is clear from the description. Further, since changes can be made to the above structure without departing from the spirit and scope of the present invention, the above description is merely an example and should not be interpreted in any limiting sense.

The claims cover all features of the invention and 7i-
It encompasses all matters within the scope of the present invention that may be omitted due to lack of language.

[Brief explanation of drawings]

FIG. 1A is a plan view of a collection room constructed in accordance with a preferred embodiment of the present invention; FIG. 1B is a side view of the pseudo-four chamber of FIG. 1A; and FIG. 2A to 20 are cross-sectional views showing the progress of the aggregation reaction occurring in the agglutination reaction chamber of FIG. 1A, and FIGS. 3A to 30 are partial enlarged views of the shaded area C. FIG. 4 is a schematic plan view showing another embodiment of the aggregation reaction chamber constructed according to the present invention; FIG. FIG. 6A is a plan view showing yet another embodiment of the reaction chamber; FIG. 6B is a plan view showing another embodiment of the aggregation reaction chamber constructed according to the present invention;
A side view of the flocculation reaction chamber in Figure. FIG. 7 shows ff1l of a central collection room constructed according to the present invention.
FIG. 8A is a side view showing an embodiment of the present invention; FIG. 8A is a plan view showing another embodiment of the aggregation reaction chamber constructed according to the present invention; FIG.
The figure is a side view of the aggregation reaction chamber in the BA diagram. 10. φ agglutination reaction chamber, 12.. Reagent, 15.. Capillary chamber, 20..-E section panel, 21..- Lower panel, 22..- Accommodation area. 23...Liquid, 28.1 aggregate, 30...Spacer, 35 fist - aggregation anti-screaming chamber, 40---h, section panel, 41@Φ・° lower panel, 45...φ@ collection Reaction chamber, 47a, 47b, 47c*ψ・Top panel, 50...-
Upper panel, 51...φ lower panel, 52...-circular opening, 55... aggregation reaction chamber. 60a, sob... Upper panel, 61... Lower panel, 63.6411. Fist spacer, 65... Coagulation reaction chamber, 73.74... Chemical substance, 75... Coagulation reaction chamber. Patent applicant Hiyu Φbui Cottingham Tl1Jga's engraving (no change in content) F/G, IC F/6.2B FI6.4 F/θθB Procedure? s13 official letter (spontaneous) November 19, 1985 Michibe Uga, Director General of the Patent Office1, Indication of the incident, Patent Application No. 236539 of 19852, Name of the invention, Aggregation reaction chamber for liquid particle reagents 3, Amendment. Person who makes the application/Relationship with case h Patent applicant address name Hiyu Bui φKottingam 4, agent 5, subject of amendment (1) drawings, /

Claims (2)

[Claims] (1) a first transparent panel having a first surface having a first predetermined area; and a second panel having a second surface having a second predetermined area; , the second surface coextends with and overlaps at least a portion of the first predetermined area, and the second panel includes the overlapping portion of the first and second surfaces. forming a capillary chamber such that when a reagent liquid is introduced into or near the capillary chamber, liquid reagent is drawn between the first surface and the second surface; An aggregation reaction chamber for a liquid particle reagent, characterized in that the chamber is disposed a predetermined distance away from the reagent. (2) The liquid particle reagent aggregation reaction chamber according to claim 1, wherein at least one of the first and second surfaces is capable of being infiltrated. The liquid particle reagent aggregation reaction chamber according to claim 1, wherein at least one of the panel and the second panel is a glass panel.