JPS6171346A - Fixed microorganizm film - Google Patents

Abstract

PURPOSE:To make it possible to perform simple determination of a volatile organic material in a sample, by laminating an oxygen and volatile-organic- material transmitting film forming a unitary body on the surface of one porous film of a microorganism fixing layer, in which the microorganism is held be tween porous films and the peripheral parts are bonded. CONSTITUTION:A culture solution of aerobic microorganism, which is cultured in a extremely suitable culture medium, is made to pass the central part of a cellulose acetate film 2, and the microorganism is attached. Then the film 2 is bonded to another film 2 through ring shaped double-side adhesive tape 4a, and a unitary body is formed. A microorganism fixing layer, in which the microorganism is sealed and held, is formed. A porous Tefron film 6 is bonded to one surface of the microorganism fixing layer by using a ring-shaped double- side adhesive tape 7a. The fixed microorganism film is mounted on a diaphragm type oxygen electrode,and a volatile material sensor can be simply constituted.

Description

[Detailed Description of the Invention] (a) Object of the Invention [Field of Industrial Application] This invention relates to an immobilized microbial membrane. More specifically, the present invention relates to an immobilized microbial membrane that can easily quantify volatile organic substances in a large number of samples when combined with a diaphragm-type oxygen electrode.

[Conventional technology]

A method using a microbial electrode in combination with a chemical microbial membrane has been used, and recently proposals have been made to measure volatile organic substances using this microbial electrode.

In this method, aerobic microorganisms, such as Trichosporon, are placed in the center between two porous membranes as shown in Figure 4.
After forming a microorganism electrode by closely contacting the immobilized microorganism membrane head on which Brasaicae and the like are immobilized on the diaphragm of the diaphragm type oxygen electrode (8), the membrane is further covered with an oxygen and volatile organic substance permeable membrane ring. This prevents direct contact between the sample liquid passing through the flow cell (9) in an aerated state and the microbial film aQ, and eliminates lower alcohols and lower This is a method in which volatile organic substances such as fatty acids are selectively permeated through microbial membranes for measurement.

[Problem that the invention seeks to solve]

However, in such coated electrodes, the oxygen electrode diaphragm □
After attaching the microbial membrane head to the top, it is further covered with a permeable membrane (5), which causes misalignment during attachment, resulting in a diameter of approximately 5 sm.
) and the microbial cell-retaining part (usually about 5 volts in diameter) in the center of the microbial membrane, which caused a problem in that the output was reduced and the cine became stable, making it impossible to perform normal table measurements. Furthermore, the coating and replacement of the permeable membrane I itself is troublesome and requires skill because O-IJing and the like are used.

This invention has been made to solve these problems, and aims to provide a composite immobilized microbial membrane that is convenient to attach and replace, and which is free from the negative effects caused by membrane displacement. It is something.

(B) Structure of the Invention Thus, according to the present invention, there is provided a microorganism-immobilized layer in which microorganisms are sandwiched in the center between two porous membranes and the peripheral parts thereof are bonded together with an adhesive; An immobilized microbial membrane is provided which is comprised of an oxygen and volatile organic substance permeable membrane which is laminated on the surface of one porous membrane and is integrated with the porous membrane by adhesion at its peripheral portion.

The immobilized microorganism membrane of the present invention sandwiches microorganisms in the center between two porous membranes and integrates an oxygen and volatile organic substance permeable membrane on one side, so that it can be placed on the membrane of a membrane-type oxygen electrode. The sensor can be easily attached to a sensor for measuring volatile organic substances. Moreover, it is convenient because the cathode part of the oxygen electrode and the microbial cell holding part are unlikely to be misaligned. As the porous membrane in this invention, those used in conventional immobilized microbial membranes can be suitably adopted, for example. , cellulose acetate membrane, polyvinyl chloride membrane, polyvinylidene chloride membrane, etc., or equivalents thereof can be used.

In addition, a circular shape is usually suitable, and the thickness is
A suitable thickness is 10 to 300 μm.

On the other hand, the oxygen and volatile organic substance permeable membrane of the present invention is preferably one that is water impermeable, such as a polyfluorocarbon or silicone membrane having micropores.

Normally, it is suitable for these micropores to be in the range of 0.1 μm to 10 μm, and a membrane having such micropores is, for example,
It can be produced by making it porous using a stretching method. The most preferred membrane is a porous Teflon membrane made porous by a stretching method and is readily available. The film thickness is 10
-300 .mu.m is suitable, and 50-80 .mu.m is preferable.

Further, adhesive wires used for integration, ordinary pressure sensitive adhesives, adhesive tapes, etc. can be used. However, these adhesive layers are preferably adjusted to have as little thickness as possible, in other words, so that the two porous membranes and the oxygen and volatile organic substance permeable membrane are in close contact with each other as much as possible.

〔Example〕

FIG. 1 is an explanatory diagram showing the structure of the immobilized microorganism membrane (1) of the present invention. In the figure, the immobilized microbial membrane (1) holds the microorganism (3) in the center between two circular cellulose acetate membranes (2) +2), and the periphery of the membrane is bonded with an adhesive (4). The microorganism immobilization layer (5) is composed of a microorganism immobilization layer (5) and a circular porous Teflon membrane (6) laminated on the lower surface of the immobilization layer (5). Porous Teflon membrane (6
) is surrounded by the cellulose acetate film (
2) is adhered to one side with an adhesive (7), and is substantially in close contact with the microorganism immobilized layer. The adhesive (7) is applied to the center of the membrane so that oxygen and volatile organic substances that permeate through the porous Teflon membrane (6) effectively contact the microorganisms (3) in the microorganism immobilization layer (5). is not formed,
It is positioned in a ring shape to face the adhesive (4) in the microorganism fixed layer (5).

Such an immobilized microbial membrane (1) can be made by, for example, as shown in FIG. After allowing microorganisms to adhere to the membrane (2) through the center, a ring-shaped double-sided adhesive tape (4a
) through the other cellulose acetate on top of it! (2
) is adhered and integrated to set up a microorganism immobilized layer that encapsulates and retains microorganisms, and a porous Teflon membrane (6) is further laminated and integrated on one side using a similar ring-shaped double-sided adhesive tape (7&). If you make it well, you can make a tong.

By attaching the immobilized microorganism membrane of the present invention to a diaphragm-type oxygen electrode, a volatile organic substance sensor can be easily constructed. An example of this configuration is shown in FIG. The volatile organic substance sensor shown in the figure makes the most of the features of the immobilized microorganism membrane of this invention.The flow cell (9) is screwed and fixed to the oxygen capacitor (8), and the immobilized microorganism [(
1) is installed. In order to create such a sensor, first, a diaphragm can is positioned so as to cover one opening of an electrode cylinder (e) having a through space, and then a diaphragm can is placed so as to press down on the periphery of the diaphragm can.
- The diaphragm (@) is fixed by fitting with a ring and then screwing and fixing the cap A@, and then the immobilized microbial membrane (1) of this invention, such as the first factor, is placed on top of the cap A at its center. 70-cell (9) is screwed so as to cover the cap B, and then the cathode (support) and anode It can be manufactured by inserting, fixing, and injecting an electrode body (product) with a part (to) and an electrolytic solution (product).In addition, in the figure, the lower part of the penetration space of the electrode tube is a tapered part that gradually narrows. Because of this, the cathode section located below the electrode body □
□□ is set so as to surely correspond to and contact the central part of the immobilized microorganism membrane (1), that is, the bacterial cell holding part. In a sensor for measuring volatile organic substances with such a configuration, a composite membrane in which an oxygen and volatile organic substance permeable layer and a microbial membrane are integrated is used, so the diaphragm of the oxygen electrode (8)
□□It can be easily attached to the top, and in conjunction with the tapered structure, it can be used for the cathode part of the oxygen electrode, the bacterial cell holding part of the microbial membrane, and the central permeation part of the M, elementary and volatile organic substance permeable membrane. correspond to each other, deviations are less likely to occur, and responsiveness is improved.

(C) Effects of the Invention As described above, the immobilized microbial membrane of the present invention can be easily mounted on the diaphragm of a diaphragm-type oxygen electrode, thereby making it possible to easily construct a sensor for measuring volatile organic substances.

The structure is such that it is difficult to cause instability or decrease in output due to misalignment between the oxygen electrode and the cathode, so even non-experts can easily handle it and it is useful. .

[Brief explanation of drawings]

FIG. 1 is a configuration explanatory diagram showing one embodiment of the immobilized microbial membrane of the present invention, FIG. 2 is an exploded perspective view of the same, and FIG. A cross-sectional view showing an example of an organic substance measuring sensor, and FIG. 4 is a configuration explanatory diagram showing a conventional electrode configuration for measuring continuous organic substances. membrane, (2)
−・・・・・・・Cellulose acetate membrane, (3)・
・・・・・・・・・Microorganisms, (4), (71・・・・
...-Adhesive, (5) ..... Microorganism fixed layer. Figure 1 Figure 2

Claims (1)

[Claims] (1) A microorganism immobilization layer that holds microorganisms in the center between two porous membranes and adheres the peripheral parts with an adhesive; and an oxygen and volatile organic substance permeable membrane which is integrated with the porous membrane by peripheral adhesion.