JPS6166146A - Ion-seed analyzing method - Google Patents

Abstract

PURPOSE:To make it possible to analyze ion seeds, which do not have ultraviolet-ray absorbing characteristics simultaneously by using the same column and measuring instrument, by using an elute having specified ultraviolet-ray absorbance, and relatively providing ultraviolet-ray transmitting characteristics in the ion seeds, which do not have the ultraviolet-ray absorbing characteristics. CONSTITUTION:A solution has specified ultraviolet-ray absorbance and includes, e.g., 4mmol/l of NaHCO3 and 4mmol/l of Na2CO3. The solution is sent to a discharged liquid container 7 through an injector 3 having connecting ports 3a-3f and a measuring pipe 4g, a separation column filled with, e.g., an anion exchange resin, and a detector 5 from a storing container 1 by a pump 2. As samples, e.g., ion seeds of NO2<->, Br<-> and NO3<-> having ultraviolet-ray absorbing property and ion pairs of Cl<->, PO4<3-> and SO4<2-> having non-absorbing property are included and eluted. The ion seeds can be detected at a positive peak. The ion pairs can be detected at a negative peak. Both ion seeds can be detected in a short time by one operation of one simple device.

Description

DETAILED DESCRIPTION OF THE INVENTION <Industrial Application Field> The present invention relates to an ion species analysis method for chromatographically analyzing ion species in a liquid to be measured using an ultraviolet absorption detector.

<Prior Art> Conventionally, when analyzing ion species in a liquid to be measured using an ultraviolet absorption detector, the ion species that can be analyzed are limited to those having ultraviolet absorption characteristics. Therefore, if ion species with and without UV absorption properties coexist in the liquid to be measured, either give up on analyzing the ion species that do not have UV absorption properties, or use other methods to detect the ion species. The analysis was conducted using a device (such as a conductivity detector).

However, using multiple different detectors for one ion species analysis has the drawbacks that the analysis operation becomes complicated and the analysis takes a long time, and the manufacturing cost of the analyzer H increases. Ta.

<Object of the Invention> The present invention has been made in view of the above situation, and its purpose is to develop an ion species that can be chromatographically analyzed using an ultraviolet absorption detector even for ion species that do not have ultraviolet absorption characteristics. The objective is to provide an analytical method.

<Summary of the Invention> A feature of the present invention is that in a method for chromatographically analyzing ion species in a liquid to be measured using an ultraviolet absorption detector, a solution having a certain ultraviolet absorbance is used as an eluent; By imparting ultraviolet transmission characteristics to ionic species that do not have absorption characteristics, it is possible to analyze even ionic species that do not have ultraviolet absorption characteristics.

<Example> Hereinafter, the present invention will be described in detail using the drawings. 1st
The figure is an explanatory diagram of the configuration of an analyzer using an embodiment of the present invention, and in the figure, 1 is, for example, 4 mmol/j of NaHC.
A tank storing an eluent consisting of a solution containing O3 and 4 m mo +/z of Na2CO3, 2 a pump for delivering the eluent at a flow rate of, for example, 2 ml/mln, 3 a first to
An injector having sixth connection ports 3a to 3f and a metering tube 3g, the internal flow path of which is connected by switching alternately between a solid line connection state and a broken line connection state in FIG. 1, 4 is filled with an anion exchange resin, for example 5 is a detector consisting of an ultraviolet absorption detector; 6 is a constant temperature bath that accommodates the separation column 4 and the detector 5 and keeps them at a predetermined temperature (for example, 40° C.);
7 is a waste liquid tank into which the liquid discharged from the detector 5 is led and stored. In the analyzer configured as above, when the pump 2 is driven, the eluent in the tank 1 drains, the pump 2 → the first injector 5. Second connection port 3a, 3b→
It flows through the flow path of separation column 4 → detector 5 → waste liquid tank 7.

In this state, the ultraviolet absorbance of the eluent is detected by the detector 5, and the detected signal is guided to a recorder (not shown) to form a baseline with a certain background (corresponding to the ultraviolet absorbance of the eluent). It's like drawing.

Next, for example, 'ppm No2''-, 10ppm B
r-, 5 ppm NO3-, 100 ppm cz-.

200 ppm p04. and 200 ppm S0
A standard solution containing 4 (hereinafter simply referred to as "standard solution") is
Inject into the metering tube 3g from the connection port 3d of the injector 3. Thereafter, the injector 3 is turned on and its internal flow path is switched from the solid line connection state in FIG. 1 to the broken line connection state.

The standard solution in the measuring tube 3g is carried by the eluent to the separation column 4, where the ionic species in the standard solution are chromatographically separated. The eluate from the separation column 4 is guided to a detector 5, where its ultraviolet absorbance is detected, and the detected signal is led to the recorder, etc., to give the chromatogram shown in FIG. In this chromatogram, ion species (
NO2LBz', and N03-) have peaks in the positive direction, and are ion species that do not have ultraviolet absorption characteristics (cc,
po3- and SO2-) have negative peaks. This is because the eluent has a certain ultraviolet absorbance, so that ion species that do not have ultraviolet absorption characteristics have relatively negative ultraviolet absorption characteristics (that is, ultraviolet transmission characteristics). Therefore, if there is an ion species that has a slightly lower ultraviolet absorbance than the eluent, that ion species will also give a peak in the negative direction.

Figure 3 shows No. 1 as an ionic species with ultraviolet absorption characteristics.
FIG. 3 is a diagram showing the results of selecting Br- and NO□- and investigating the linear relationship between the concentration of these species I and the peak height using the above-mentioned embodiment of the present invention. From this figure,
When the concentration of ionic species having ultraviolet absorption characteristics is analyzed using the embodiment of the present invention, it is found that very good linearity can be obtained. FIG. 4 shows Ct''
p04. and 502- are selected, and the linear relationship between the concentration of these ion species and the peak height is a diagram showing the results of an investigation using the above-mentioned embodiment of the present invention. From this figure, it can be seen that when analyzed using the example of the present invention, very good linearity can be obtained even for ion species that do not have ultraviolet absorption characteristics.

<Effects of the Invention> As described above in detail, according to the embodiments of the present invention, an eluent having a certain ultraviolet absorbance is used to impart ultraviolet transmission properties to ionic species that do not have ultraviolet absorption properties. This configuration makes it possible to chromatographically analyze ion species that do not have ultraviolet absorption characteristics using an ultraviolet absorption detector. In addition, as shown in the chromatogram in Figure 2, ion species with ultraviolet absorption characteristics give a peak in the positive direction, while ion species without ultraviolet absorption characteristics give a peak in the negative direction, which provides new information regarding the qualitative properties of ion species. There is also the advantage that one is added.

[Brief explanation of the drawing]

FIG. 1 is an explanatory diagram of the configuration of an analyzer using an embodiment of the present invention;
FIG. 2 is a chromatogram prepared using the apparatus shown in FIG. 1, and FIGS. 3 and 4 are diagrams showing the linearity of ion species concentration and peak height. 1.7... Tank, 2... Pump, 3... Injector, 3g... Measuring tube, 4... Separation column, 5...
Detector, 6... constant temperature bath.

Claims (2)

[Claims] (1) Collect a certain amount of the liquid to be measured and transport it to a separation column using an eluent to separate the ionic species in the liquid, and detect the ultraviolet absorbance of the liquid eluted from the separation column to separate the ionic species. In the analysis method, a solution having a certain ultraviolet absorbance is used as the eluent, and ionic species that do not have ultraviolet absorption characteristics are made to have ultraviolet transmission characteristics, so that ionic species that do not have ultraviolet absorption characteristics or An ion species analysis method characterized by simultaneously analyzing ion species that have ultraviolet absorption characteristics and ion species that do not. (2) The solution having a certain UV absorbance is 4 mm
ol/l NaHCO_3 and 4 mmol/l Na_2C
The ion species analysis method according to claim (1), which comprises a solution containing O_3.