JPS6144822A - Health promoting and keeping agent containing chondroitin sulfate - Google Patents

Abstract

PURPOSE:The titled pharmaceutical consisting of chondroitin sulfate, or a salt thereof, lecithin and protein, and having angifatigue action, protecting action on gastric mucosa and improving action on constipation. CONSTITUTION:A health promoting and keeping agent containing 1-40pts.wt. chondroitin sulfate or a salt thereof, 0.5-20pts.wt. lecithin and 40-98.5pts.wt. protein and if desired <=10pts.wt., based on 100pts.wt. total weight of main components, vitamin, vegetable extract, e.g. ginseng extract, various amino acids, various minerals, etc. The dosage form is solution, granule, table, troche or capsule, etc. The dose thereof is 1-2g at a time, and administered 1-3 times a day. The agent is widely applied to maintenance of physical strength, fatigue recovery, unpleasant feeling in the gastric and hypogastric regions, menopausal disorder, drunken sickness, nourishment and tonicity, weak physical constitution, etc.

Description

[Detailed Description of the Invention] [Field of Application in JT Industry] The present invention relates to a health promoting/maintaining agent, and more specifically, a health promoting agent containing chondroitin sulfate that has anti-fatigue effects, gastric mucosal protective effects, and constipation improving effects.・Regarding maintenance agents.

[Prior Art] Chondroitin sulfate is a type of mucopolysaccharide that is generally distributed in the connective tissues of animals, mainly cartilage, and in the tissues, it binds to proteins and exists as proteoglycans.

Chondroitin sulfate is usually used as a sodium salt, and pharmacologically it is known to have lipid serum clarifying action, anticoagulant action, cell activation action, detoxification action, analgesic action, and the like.

However, it has not yet been applied as a health promotion/maintenance agent.

Therefore, as a result of extensive research into the effective use of chondroitin sulfate as a health-promoting and maintenance agent, the present inventors found that a composition containing chondroitin sulfate or its salt, lecithin, and protein in a certain proportion has an anti-fatigue effect. , has both gastric mucosal protective effect and constipation improving effect, and by improving bowel movement, relieves the discomfort associated with constipation, increases appetite, protects the stomach wall, improves nutritional intake, and activates metabolism. The present inventors have discovered that it has excellent properties as a health promotion/maintenance agent, which improves and maintains physical strength, leading to the completion of the present invention.

[Structure of the Invention] The health promoting/maintaining agent of the present invention is characterized by comprising 0.5 to 20 parts by weight of lecithin and 40 to 98.5 parts by weight of protein.

In the present invention, chondroitin sulfate may be used in purified form or in the form of proteoglycan F1. In this case, the amount of chondroitin sulfate equivalent in the proteoglycan is within the above range, and the total amount of protein in the proteoglycan and newly added protein may be within the above range.

How to obtain chondroitin sulfate in the form of proteoglycans 1. For example, use the cartilage of aquatic animals such as sharks and whales, mammals such as cows and pigs, or birds, etc. as raw materials, and use the neutral salt method, alkaline method, enzyme method, autoclave method, etc. It may be extracted according to a known method such as the method, remove fat, solids, etc., and then dried.

Furthermore, after removing the fat fist solids, the product is further subjected to protein removal treatment using a proteolytic enzyme and purified according to a known method by alcohol precipitation, whereby chondroitin sulfate or its salt can be obtained in a purified state.

As the salt of chondroitin sulfate, sodium salt is usually used, but calcium salt, potassium salt, etc. may also be used.

As the protein, in addition to those derived from proteoglycans, protein derived from egg white or whole egg white, milk protein, animal protein such as seratin, and vegetable protein such as soybean protein can be used.

If the blending ratio of chondroitin sulfate or its salt or lecithin is less than the above-mentioned limit, the anti-fatigue effect and gastric mucosal protective effect will be insufficient.

In addition to the main ingredients, chondroitin sulfate or its salt, lecithin, and protein, the health promotion/maintenance agent of the present invention contains hitamines A, B, B2. B6, B12, C, D, E,
Pantothenic acid, nicotinamide, biotin, folic acid, inositol, taurine, plant extracts such as carrot extract, various amino acids, various minerals, etc. can be blended as appropriate. The total amount of lecithin and protein is preferably 10 parts by weight or less per 100 parts by weight.

The health promotion/maintenance agent of the present invention is usually used in the form of a liquid, granule, tablet, troche, or capsule.

When using, the product of the present invention is usually administered at a dose of 1 to 2 g at a time.
Take 1 to 3 times per day.

[Effects of the Invention] The health promoting/maintaining agent of the present invention has anti-fatigue effects, gastric mucosal protective effects, and constipation improving effects, and is effective in maintaining normal physical strength, recovering from fatigue, discomfort in the back and lower abdomen, menopausal symptoms, and nausea. drunkenness
It is widely applied to nourish, strengthen, and weaken the body.

[Examples of the Invention] Hereinafter, the present invention will be explained in more detail with reference to Examples and Prescription Examples, but these are not intended to limit the scope of the present invention in any way.

Example (1) Preparation of specimens The following liquid formulations of the products of the present invention (specimens A and B) and liquid formulations of comparative examples (specimens C and D) were prepared.

Sample A: Sodium chondroitin sulfate (molecular weight approximately 10
,000) 15%, lecithin 5% and soy protein 80%
Sample B: Cartilage extract 85%
(Sodium chondroitin sulfate 13%, protein 82%
A 10% aqueous cartilage extract of a composition containing 5% lecithin and 5% lecithin is obtained by keeping pig tracheal cartilage at 121°C for 120 minutes, centrifuging it, and removing the fat fist solids. This product contained 13.7% sodium chondroitin sulfate and 88.3% protein.

Sample C: 1.5% sodium chondroitin sulfate aqueous solution Sample D: 0.5% lecithin suspension (2) Anti-fatigue effect (a) Experimental method Ten ddY male mice (5 weeks old) were used in each group. The specimens were given free access to water.

During the pre-breeding week, a running test was conducted for 30 seconds every day, and those with good running performance were selected for the experiment. Pyrogenic substances (E
0.4 ml of physiological saline solution containing 0.2 gg/m (prepared from Scherichia coli P-020, manufactured by Teikoku Kinki) was injected once a day through the tail vein for 8 consecutive days, and 1 hour after the final injection. A driving test was then conducted.

The running test was carried out using a forced running device equipped with an electrical stimulation device under the conditions of an inclination angle of 10' and a belt speed of 25 m/min, and electrical stimulation was applied as necessary to force the test vehicle to run.

Controls received water.

(b) Results Table 11 Those who completed the forced running test completed the run with plenty of time without receiving electrical stimulation.

As is clear from the above results, the group in which the product of the present invention, that is, specimen A or specimen B, was given to mice whose bodies had been weakened by administering a pyrogenic substance in advance clearly completed more than the other groups. They were large in number, had a long average mileage, and showed strong resistance to fatigue. In addition, the mice in the sample A and sample B administration groups were
All the hair was smoother than the other groups.

(3) Protective effect on gastric mucosa (a) Experimental method Five male Wistar rats (body weight 165-tsog) were used in each group. Fasted at 10 o'clock the day before the experiment, and then at 15 o'clock the day before the experiment.
One sample of Marabun was orally administered using a sonde to a rat that was watered at the same time, and after 30 minutes, anhydrous ethanol was orally administered in the same manner. After -1 hour, the stomach was removed and the sample was placed in a 1% formalin solution for 30 minutes. After soaking, a large incision was made in the gastric mucosa, and the total area calculated from the number of lesions formed on the gastric mucosa and the length x width of each lesion was defined as the damaged area. Controls received physiological saline.

(b) Results Table 2 As is clear from the results above, the area of gastric mucosal damage caused by alcohol was smaller in the group given the product of the present invention, that is, sample A or sample B, than the other groups. It showed strong resistance to damage.

(4) Effect on constipation Among people suffering from constipation, 15 people had bowel movements once a week;
Similarly, 23 people who twice a week were given solid food corresponding to Sample A or Sample B at a dose of 1.58 per time, three times a day.
The results of taking the drug every day for 7 days were as follows.

Table 3 Constipation-improving effect Note 1): People who communicate with the drug every day from the 2nd to 3rd day of starting treatment 2)
: Every other day or every day from the 3rd day of administration.As is clear from the above results, all of the products of the present invention showed a remarkable improvement effect on constipation.

(5) Acute toxicity The acute toxicity value of the product of the present invention, which is composed of 15% sodium chondroitin sulfate, 5% lecithin, and 80% cartilage-derived protein, was measured by oral administration, and the following results were obtained.

As is clear from the above results, the product of the present invention has extremely low toxicity.

Prescription example Lecithin 3g Dried egg whites 77g were mixed and made into granules according to a conventional method.

Formulation Example 2 5 g of lecithin was mixed, made into granules according to a conventional method, and filled into capsules.

Formulation example 3 Lecithin 3g Gelatin Ei 7g Retinol palmitate 9,000 vitamin A units Thiamine hydrochloride
1100ff1 vitamin B2
80mg vitamin 86 80
mg vitamin CI, 000mg calcium pantothenate 5hg nicotinamide 200B tocopherol acetate 50mg ergocalciferol 8
,000 international units were mixed and made into granules according to a conventional method.

Prescription Example 4 Thiamine hydrochloride 50mg Vitamin B 2 to 100g of the mixture of Prescription Example 2
25mg vitamin B6
25mg calcium pantothenate 25m
gNicotinic acid amide 100mgMethionine 10hgSalted lysine
100mg carnitine 200mg
300 mg of glycerophosphoric acid and 50 mg of calcium ginseng extract were added and mixed, and water was added to make the total amount into 1,000 layers.

Manual continuation supplementary book August 30, 1982

Claims (1)

[Claims] 1 to 40 parts by weight of chondroitin sulfate, or its salt 0.5 to 20 parts by weight of lecithin and 40-98.5 parts by weight of protein A health promotion/maintenance agent characterized by comprising: