JPS6135798A - Automatic determination of biota - Google Patents

Automatic determination of biota

Info

Publication number
JPS6135798A
JPS6135798A JP15834384A JP15834384A JPS6135798A JP S6135798 A JPS6135798 A JP S6135798A JP 15834384 A JP15834384 A JP 15834384A JP 15834384 A JP15834384 A JP 15834384A JP S6135798 A JPS6135798 A JP S6135798A
Authority
JP
Japan
Prior art keywords
biota
image
divided
camera
aeration tank
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP15834384A
Other languages
Japanese (ja)
Inventor
Akaru Furusato
古里 明瑠
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nishihara Environment Co Ltd
Original Assignee
Nishihara Environmental Sanitation Research Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nishihara Environmental Sanitation Research Corp filed Critical Nishihara Environmental Sanitation Research Corp
Priority to JP15834384A priority Critical patent/JPS6135798A/en
Publication of JPS6135798A publication Critical patent/JPS6135798A/en
Pending legal-status Critical Current

Links

Classifications

    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00Technologies for wastewater treatment
    • Y02W10/10Biological treatment of water, waste water, or sewage

Landscapes

  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Activated Sludge Processes (AREA)
  • Image Processing (AREA)

Abstract

PURPOSE:To enable the accurate determination of the biota in a specimen liquid, by dividing the expanded image of the biota of the specimen liquid photographed with a camera, comparing the image in each divided section with the preliminarily stored reference images, and specifying the kind of the microorganisms in the section. CONSTITUTION:The state of the biota in an aeration tank 1 is photographed properly with the camera 2 of the monitoring TV apparatus 3, and the expanded image is displayed on the monitor TV5 via a transmitter 4. The image signal is divided with a CPU7, and each divided image is compared with the preliminarily stored reference 6 to specify the kind of microorganism. The biota of the specimen liquid can be determined accurately, and a proper control of the aeration tank can be carried out by this process.

Description

【発明の詳細な説明】 〔産業上の利用分野〕 この発明は被処理液の生物相をカメラで拡大撮影した画
面を区分し、区分毎に予め記憶されたリファレンスと照
会比較して画像内微生物の特定を行う生物相の自動判定
方法に関する。
[Detailed Description of the Invention] [Industrial Application Field] This invention divides the screen of the biota of the liquid to be treated enlarged and photographed with a camera, and compares the microorganisms in the image with pre-stored references for each division. This invention relates to an automatic biota determination method for identifying biota.

〔従来技術〕[Prior art]

例えば、活性汚泥処理におい【は、処理の主役は曝気槽
中の活性汚泥微生物であり、この微生物の環境条件を整
えることによって、初めて活性汚泥処理が円滑に行われ
る。
For example, in activated sludge treatment, the main role of the treatment is the activated sludge microorganisms in the aeration tank, and activated sludge treatment can only be carried out smoothly by adjusting the environmental conditions for these microorganisms.

そして、従来は曝気槽内のPH,no等を微生物の環境
の指標として利用していた。また、曝気槽内の汚泥微生
物を顕微鏡によって観察し、曝気槽内の状態を把握する
ことも行われていた。
Conventionally, the PH, NO, etc. in the aeration tank have been used as indicators of the microbial environment. In addition, sludge microorganisms in the aeration tank were observed using a microscope to understand the condition inside the aeration tank.

しかし、この顕微鏡による方法は、非常に有効な手段で
あるが、その観察は熟練を要し、しかも煩雑な手間を必
要とするという欠点があった。
However, although this method using a microscope is a very effective means, it has the drawback that the observation requires skill and labor.

〔発明の概要〕[Summary of the invention]

この発明は上記欠点を解消するためになされたものであ
って、被観察液中の生物相をモニターテレビカメラ装置
で監視し、拡大された画面を複数に分割して、その区分
毎忙予め記憶されたリファレンスと比較し生物相の種類
の判定を行うことによって、被観察液を正確に把握でき
る生物相の自動判定方法を提供することを目的とする。
This invention was made in order to solve the above-mentioned drawbacks, and the biota in the liquid to be observed is monitored using a monitor TV camera device, the enlarged screen is divided into a plurality of parts, and each division is memorized in advance. The purpose of the present invention is to provide an automatic biota determination method that can accurately grasp the observed liquid by comparing the type of biota with the reference obtained.

〔発明の実施例〕[Embodiments of the invention]

以下、この発明の一実施例について、図面を参照して説
明する。
An embodiment of the present invention will be described below with reference to the drawings.

第1図に示すように、曝気槽1の内部には、曝気槽1中
の活性汚泥の生物相を拡大撮影するNVバイオスコープ
等のカメラ2が浸漬されている。
As shown in FIG. 1, a camera 2 such as an NV bioscope is immersed inside the aeration tank 1 to take an enlarged picture of the biota of the activated sludge in the aeration tank 1.

このモニターテレビ装置3のカメラ2は、撮影した画像
を画像信号として出力する変換器4を介してモニターテ
レビ5に接続されている。
The camera 2 of this monitor television device 3 is connected to a monitor television 5 via a converter 4 that outputs a photographed image as an image signal.

このモニターテレビ5は、第2図に示すようにその画像
が複数に分割され、リファレンス6に接続した中央処理
装置(cpu)7に接続されている。このリファレンス
6には、複数の指標生物のパターンが予め記憶され、中
央処理装置(CPU)7では区分された画像毎にこのリ
ファレンス6との比較を行う。
The monitor television 5 has an image divided into a plurality of parts as shown in FIG. 2, and is connected to a central processing unit (CPU) 7 connected to a reference 6. Patterns of a plurality of indicator organisms are stored in advance in this reference 6, and a central processing unit (CPU) 7 compares each divided image with this reference 6.

〔作用〕[Effect]

以上の構成によれば、曝気槽1中の生物相の状態は、モ
ニターテレビ装置3のカメラ2によって適切撮影され、
その拡大画像は変換器4を介してモニターテレビ5に映
し出される。そして、画像信号は中央処理装置(CPU
)?で分割され、その区分された画面毎に予め記憶され
たす7アレンス6との照合を行い、微生物の特定(固定
)を行う。ここで、微生物特定の画像処理は、例えば、
次のような手順で行われる。
According to the above configuration, the state of biota in the aeration tank 1 is appropriately photographed by the camera 2 of the monitor television device 3,
The enlarged image is displayed on a monitor television 5 via a converter 4. The image signal is then processed by the central processing unit (CPU).
)? The microorganisms are identified (fixed) by comparing each divided screen with a pre-stored image 6. Here, microorganism specific image processing is performed by, for example,
The procedure is as follows.

■画面全体の中から生物体と考えられるものを選び出す
。これは、例えば輪郭のはつきシした独立した物体を選
び出すことによって行われる。
■Select things that are considered to be living organisms from the entire screen. This is done, for example, by selecting independent objects with sharp outlines.

■次に、この物体の大きさ、外形を特定し、次忙特徴部
分の特定を行う。例えば、鞭毛があるかとか、口部が全
体圧対してどの位の大きさとか、軸糸があるか等の特定
を行う。
■Next, the size and external shape of this object are specified, and the characteristic features of the object are specified. For example, we identify whether there are flagella, how large the mouth is relative to the overall pressure, and whether there is an axoneme.

■この大きさ、外形、特徴部分をリファレンス(予め、
必要な生物体の特徴を画像処理の上、cpUメモリ一部
に内蔵してあって、ページングして取り出せるようにス
トックされているもの)する。
■Reference this size, external shape, and characteristic parts (in advance,
After image processing, the necessary characteristics of the living body are stored in a part of the CPU memory and can be retrieved by paging.

■そして、この照会比較を複数回(内蔵されているペー
ジ数に合わせて)行い、生物種毎の個数をカウントし、
その結果をモニターテレビ5に出力する。(ここで1回
の画像が被観察液何rnlVc対応するかカメラ2の形
状によって決まるので、生物の個体数はl ml当たシ
のものに換算するとよい。
■Then, perform this query and comparison multiple times (according to the number of built-in pages), count the number of each species,
The results are output to the monitor television 5. (Here, the shape of the camera 2 determines how many rnlVc of the liquid to be observed corresponds to one image, so the number of organisms should be converted to the number of organisms per ml.

また、このサンプリングと、照会を多数回繰り返してか
ら、データ処理することによって、よシ正確な結果が得
られる。
Also, by repeating this sampling and query many times before processing the data, more accurate results can be obtained.

この際、モニターテレビ5の分割された区切りから隣シ
画面へ連続している場合には、その対象については照会
を保留しておき、隣シの画面と一緒にした情報を用いて
照会比較を行うとよい。さらK、画面、の分割区分は、
何種類か変更使用できるよ5KL、生物種によって区分
を変更するとよい◇つまシ、リファレンス6を大型、中
型、小型などに分けておき、大型のときは例えば小型の
時の4倍の区分を一単位として照会を行う。
At this time, if there is a continuation from the divided section of the monitor TV 5 to the adjacent screen, the query for that target is suspended and the query is compared using the information combined with the adjacent screen. It's good to do. Furthermore, the division of the screen is as follows:
You can use several different types of 5KL, it is better to change the classification depending on the species ◇ Divide the reference 6 into large, medium, small, etc., and when large, for example, divide four times as many categories as small. Inquire as a unit.

また、分割された画面中に固形物が見いだせない場合に
は、照会比較は行わず、そこには微生物がいなかったこ
との信号を出力する。
Furthermore, if no solid substance is found in the divided screen, no inquiry comparison is performed and a signal indicating that there are no microorganisms is output.

さらに、判定の種類についても、原生動物、後生動物等
の生物種の特定とは別にフロックサイズ密度等も判定で
きるようにするとよい。
Furthermore, regarding the type of determination, it is preferable to be able to determine the floc size density, etc., in addition to identifying biological species such as protozoa and metazoa.

なお、この実施例では、生物相を観察する対象として曝
気槽の活性汚泥としたが、接触曝気処理槽、嫌気性処理
槽、発酵槽、バイオリアクター等の生物観察に利用して
もよいことは、もちろんである。
In this example, the activated sludge in the aeration tank was used as the target for observing biota, but it may also be used for biological observation in contact aeration treatment tanks, anaerobic treatment tanks, fermentation tanks, bioreactors, etc. , of course.

〔発明の効果〕〔Effect of the invention〕

以上のように、この発明によれば、被測定液中の生物相
をモニターテレビカメラ装置で監視し、拡大された画面
を複数に分割して、区分毎に予め記憶されたリファレン
スと比較し生物相の種類の判定を行うことによって、被
観察液の生物相を正確に把握でき、適切な制御が行える
等の極めて優れた効果がある。
As described above, according to the present invention, the biota in the liquid to be measured is monitored using a monitor TV camera device, the enlarged screen is divided into a plurality of sections, and each section is compared with a pre-stored reference. By determining the type of phase, the biological phase of the observed liquid can be accurately grasped, and there are extremely excellent effects such as being able to carry out appropriate control.

【図面の簡単な説明】[Brief explanation of drawings]

第1図はこの発明が適用される装置の一実施例の縦断面
図、第2図は同説明図を示す。 2・・・カメラ、3・・・モニターテレビ装置、5・・
・モニターテレビ、6・・・リファレンス。 特許出願人   株式会社西原環境衛生研究所(外2名
) 図面の浄11:(内容(:変更なし) 第1図 第2図 手続補正書(自発)゛ 昭和59年8・−八  日
FIG. 1 is a longitudinal cross-sectional view of an embodiment of an apparatus to which the present invention is applied, and FIG. 2 is an explanatory view of the same. 2...Camera, 3...Monitor TV device, 5...
・Monitor TV, 6...Reference. Patent Applicant Nishihara Environmental Health Research Institute Co., Ltd. (2 others) Drawing Purification 11: (Contents (: No changes) Figure 1 Figure 2 Procedural Amendment (Voluntary) August 1980-8

Claims (1)

【特許請求の範囲】[Claims] 被測定液をカメラで拡大撮影して得られた画像を複数分
割する工程と、この分割された画像をその区分毎に、予
め記憶されたリフアレンスと比較を行い、上記画像内微
生物の特定を行う工程とを備えた生物相の自動判定方法
A process of dividing the image obtained by enlarging the liquid to be measured with a camera into multiple parts, and comparing the divided images for each division with pre-stored references to identify the microorganisms in the image. An automatic biota determination method comprising a process.
JP15834384A 1984-07-28 1984-07-28 Automatic determination of biota Pending JPS6135798A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP15834384A JPS6135798A (en) 1984-07-28 1984-07-28 Automatic determination of biota

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP15834384A JPS6135798A (en) 1984-07-28 1984-07-28 Automatic determination of biota

Publications (1)

Publication Number Publication Date
JPS6135798A true JPS6135798A (en) 1986-02-20

Family

ID=15669565

Family Applications (1)

Application Number Title Priority Date Filing Date
JP15834384A Pending JPS6135798A (en) 1984-07-28 1984-07-28 Automatic determination of biota

Country Status (1)

Country Link
JP (1) JPS6135798A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08197084A (en) * 1995-01-26 1996-08-06 Meidensha Corp Biological phase diagnosis support system
JPH1024283A (en) * 1996-07-10 1998-01-27 Meidensha Corp Filtration obstructing microorganism monitor
US6251624B1 (en) 1999-03-12 2001-06-26 Akzo Nobel N.V. Apparatus and method for detecting, quantifying and characterizing microorganisms

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5978681A (en) * 1982-10-26 1984-05-07 Haruyuki Kawahara Automatic calculator of viable cell count in cultivation

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5978681A (en) * 1982-10-26 1984-05-07 Haruyuki Kawahara Automatic calculator of viable cell count in cultivation

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08197084A (en) * 1995-01-26 1996-08-06 Meidensha Corp Biological phase diagnosis support system
JPH1024283A (en) * 1996-07-10 1998-01-27 Meidensha Corp Filtration obstructing microorganism monitor
US6251624B1 (en) 1999-03-12 2001-06-26 Akzo Nobel N.V. Apparatus and method for detecting, quantifying and characterizing microorganisms
US6416969B2 (en) 1999-03-12 2002-07-09 Akzo Nobel N.V. Susceptibility plates for microbial antibiotic susceptibility testing

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