JPS61274269A - Reagent distributor - Google Patents

Abstract

PURPOSE:To enable a reagent distribution work to be handled in a short time, by holding a reagent bottle radially on a turret-shaped bottle holder while first and second reagents are distributed at a specified timing through a rotating pipet exclusively provided therefor. CONSTITUTION:First and second reagent distributors D1 and D2 are arranged between a reactor holder B and a bottle holder 6 to face each other. They are made up of pipets P1 and P2 and a pump for suction and discharge provided to suck and discharge reagents, a transfer unit which rotatates and transfers the pipets P1 and P2 to washing positions C1 and C2 via first and second reagent discharge positions beta and gamma from the first reagent suction position (a) or the second reagent suction position (b) while vertically moving them P1 and P2 at the positions (a), (b), beta, gamma, C1 and C2 and a washer for washing the pipets P1 and P2 at the washing positions C1 and C2. The operation of sucking reagents of the second reagent distributor D2 is driven and controlled to work with a time difference from the first reagent distributor D1.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a reagent dispensing device suitable for an automatic analyzer that performs biochemical analysis or immunological analysis.

[Prior art and its problems]

Conventional reagent dispensing devices of this type have a plurality of reagent bottles arranged in two lines in a straight line, and the reagents are drawn up and
There is a known method in which a reagent corresponding to a measurement item is dispensed into a reaction container by moving two discharge pipettes back and forth in a straight line in accordance with the reagent dispensing timing.

However, in the above-mentioned conventional reagent dispensing device, the pipette is configured to reciprocate in a straight line, which lengthens the reagent dispensing time, lengthens the analysis processing time, and reduces the number of samples processed per hour. In addition, since the reagent bottles are arranged in series, when there are many analysis items, the overall size of the apparatus becomes large.

In order to solve this problem, in the past, multiple reagent bottles were held radially in a turret-like bottle holder, and the bottles were rotated and transferred to the reagent dispensing position according to the measurement item, and measurements were taken at the same position. There has also been proposed a system in which a required amount of a first reagent and a second reagent corresponding to an item are aspirated using a rotatably transferred pipette, and the reagents are discharged and dispensed into a reaction container.

However, even with such a conventional reagent dispensing device, the first reagent and the second reagent must be dispensed at the required timing with a single pipette, and the first reagent and the second reagent need to be dispensed at different timings. Dispensing the second reagent and the second reagent with a single pipette has the problem of being very complicated because the drive control of the pipette and the bottle holder must be speeded up.

[Purpose of the invention]

This invention was devised in view of the current situation, and its purpose is to significantly reduce the size of the entire automatic analyzer, to easily control the drive, and to provide a first The present invention aims to provide a reagent dispensing device with a simple configuration that can quickly and easily dispense a reagent or a second reagent.

[Structure of the invention]

In order to achieve the above object, the present invention includes a reagent dispensing device that includes a plurality of reagent bottles containing a first reagent or a second reagent corresponding to a measurement item, and a talent that holds the reagent bottles. a holder rotation control device that rotationally controls the bottle holder to transfer a reagent bottle containing a reagent corresponding to a measurement item to a first reagent dispensing position and a second reagent dispensing position; A first reagent dispensing device that aspirates a required amount of a first reagent in a reagent bottle that has arrived at a reagent dispensing position and dispenses it into a reaction container; and a second reagent dispensing device that aspirates a required amount of reagent and dispenses it into a reaction container.

〔Example〕

Hereinafter, the present invention will be described in detail based on an embodiment shown in the accompanying drawings.

FIG. 1 is a plan view schematically showing the configuration of a single-multi type automatic analyzer X to which a reagent dispensing device G according to an embodiment of the present invention is applied. is composed of a talent-shaped sampler S, a reaction container holder B arranged side by side on the left side of the sampler S, and a reagent dispensing device G arranged on the left side of the holder B.

The reagent dispensing device G includes a reagent supplying device R, a first reagent dispensing device D1, and a second reagent dispensing device D2, and the reagent dispensing device R supplies the first reagent corresponding to the measurement item. Or a plurality of reagent bottles 5 containing the required amount of the second reagent.
and a turret-shaped bottle holder 6 that holds these reagent bottles 5 in a radial manner, and controls the forward and reverse rotation of this bottle holder 6 to move the reagent bottle 5 to the first reagent dispensing position a and the second reagent dispensing position. and a holder driving device #7 for transporting the holder.

In addition, the first and second reagent dispensing devices D1. Dz is arranged oppositely between the reaction container holder B and the bottle holder 6,
Pipettes Pl and P2 for aspirating and discharging reagents and a suction/discharge pump (not shown), and these pipettes Pl and Pz are placed between the first reagent suction position a or the second reagent suction position and the first and second reagent discharge positions. β.

A transfer device (not shown) that rotatably transfers the pipette p1 to the washing position C1, C2 after passing through γ, and moves the pipette p1゜Pz up and down at each of the above-mentioned positions a, b, β, r, Cx, and Cz;
It consists of a cleaning device (not shown) that cleans at P1ePzk cleaning position CI and Cz.
The configurations and operations of the PI, Pz, pipette transfer device, and cleaning device are similar to those of known pipette devices, so detailed explanations thereof will be omitted here.

Therefore, the bottle holder 6 is rotated by the reaction container holder B, which will be described later, and the reaction container A has arrived at the first dispensing position.
A required amount of the first reagent corresponding to the measurement item is aspirated and dispensed into the chamber 3 via the first reagent dispensing device DI, and a second reagent disposed opposite to the first reagent dispensing device DI is The dispensing device D2 is configured to aspirate and dispense a required amount of the second reagent corresponding to the measurement item into the reaction container A4 that has arrived at the second dispensing position γ by controlling the rotation of the reaction container holder B. The drive is controlled in synchronization with Ih, D2 and reaction vessel holder B. Further, the reagent suction operation of the second reagent dispensing device D2 is controlled to operate with a time difference from that of the first reagent dispensing device D1. This is the first dispensing position β
When the reagent bottle 5a corresponding to the measurement item is transferred and stopped, the reagent in the reagent bottle 5b that is stopped at the second dispensing position is transferred to the reaction container that has arrived at the second dispensing position. If the second reagent does not match the analysis item of the blood sample in 4, this is to avoid hindrance to controlling the rotation of the bottle holder 6 to transfer the matching second reagent to the same position. be.

Next, another configuration of the automatic analyzer X suitable for applying the reagent dispensing device G will be described.

In the sampler S, a required amount of the specimen (serum) to be measured is stored in a plurality of sample containers 1, and each sample container 1 is held in a turret-shaped sample holder 2 at equal intervals. The device intermittently transports it to the dispensing position at predetermined timing.

When a predetermined sample container 1 is transferred to a dispensing position in this way, the sample in the same container 1 is transferred to a plurality of reaction containers A that are stopped at a sample dispensing position α (to be described later) via a sample dispensing device C. My AI dispenses the required amount.

The reaction vessel holder B is formed into a ring shape, and as described above, the required number of reaction vessels A are removably accommodated in bottomed holes 20 that are opened at equal intervals as shown in FIG. ing. Further, in the hole 20 of this reaction vessel holder B, a light guide hole 21 is formed to pass through the side wall of the holder in a horizontal direction perpendicular to the long axis direction of the reaction vessel holder B, and each of these light guide holes 21 is opened so that its axis coincides with the optical axis of the light source at the optical measurement position.

The reaction vessel holder B configured in this manner is rotationally controlled by a drive device not shown. In other words, the same holder B
is a diagram of the reaction vessel Axe'fE1 in which sample dispensing has been completed at sample dispensing position α.The reaction vessel Axe'fE1 is scanned and moved counterclockwise to the first reagent dispensing position β, and at the same position β, the first reagent corresponding to the measurement item is After the reagent dispensing operation has been completed, the reaction vessel Al is then rotated and controlled to be scanned and transferred clockwise in FIG. 1 to a position ε which is one step ahead of the f-nipple dispensing position α. By this forward/reverse scan operation, the reaction container A1 is intermittently moved one step at a time toward the second reagent dispensing position γ direction.

Note that E in FIG. 1 indicates a stirring device, which stirs and mixes the blood sample, etc. in the reaction vessel A2 in the stirring position, and its structure and operation are the same as those of known stirring devices, so a detailed explanation thereof will be provided. is omitted here.

The optical measuring device F includes a closed cylindrical rotating chamber 10 and a light source 1.
The light source chamber L2 has a built-in light source chamber L2. The cylindrical rotation chamber 10 has an outer diameter slightly smaller than the inner diameter of the reaction vessel holder B, is coaxial with the holder B, and rotates at a required angle and at a required speed regardless of the rotational operation of the holder B. Rotates back and forth. This rotation angle is at least the first
The rotation is controlled to perform a scanning operation at an angle θ between the reagent dispensing position β and the second reagent dispensing position γ. Inside the cylindrical rotation chamber 1o, which is driven and controlled in this way, there are reflections g! arranged on the Rowland circle 13. 4, and a plurality of mirrors (three in the figure, but not limited to this) that are arranged at a predetermined wavelength position of the light source light reflected by the reflecting mirror [4 and receive the predetermined wavelength light.

) and a diffraction grating device 16 comprising a light receiving element body 15.
is folded inwardly, and a light source light entrance hole 18 is provided in the peripheral wall 17 of the same room 1o, where the light source light enters, penetrating the peripheral wall 17, and the axis of the circumference 18 is
It is arranged so as to be coaxial with the axis of the light guiding hole 21 . A light source chamber 12 in which the light source 11 is installed is connected to the chamber 10 along the outer circumferential wall of the holder B by a connecting body (not shown) that extends from the cylindrical rotation chamber 10 bypassing the reaction container holder B. It is connected so that it rotates together with the

Therefore, after the required amount of serum specimen is dispensed at the sample dispensing position α, the reaction vessel AI held in the reaction vessel holter "B" is scanned and transferred to the first reagent dispensing position β,
At the same position β, the first reagent corresponding to the measurement item is transferred from the corresponding reagent bottle 5a to the first reagent dispensing device DI (bipen)P
After the required amount is dispensed via I, and then scanned and transferred to the transfer position ε, it is transferred one pinch at a time in the direction of the optical measurement position by each of the above-mentioned scanning operations, and then the stirring device E
After stirring at the second reagent dispensing position γ, a required amount of the second reagent corresponding to the measurement item is dispensed from the corresponding reagent bottle 5b via the Vivent Pzk of the second reagent dispensing device D2, and then the second reagent corresponding to the measurement item is dispensed into the optical measuring device F. After the predetermined optical measurements have been carried out on all of the reaction vessels A held in the reaction vessel holder B, these reaction vessels A are cleaned in a separately provided cleaning device and reused. It will be done.

〔Effect of the invention〕

As explained above, the present invention has a structure in which reagent bottles are held radially in a tower-shaped bottle holder, and a first reagent and a second reagent are dispensed at predetermined timings through respective rotating pipettes. Because of this configuration, reagent dispensing work can be processed in a short time, and drive control corresponding to the timing of reagent dispensing is easy, and the configuration is simple and can be provided at low cost. Since the bottles are held in a radial manner, the entire automatic analyzer can be made smaller.

[Brief explanation of the drawing]

The drawings show one embodiment of the present invention, and FIG. 1 is a plan view schematically showing the configuration of a nine-single multi-type automatic analyzer to which a reagent dispensing device according to this embodiment is applied. . G... Reagent dispensing device Dl... First reagent dispensing device D2... Second reagent dispensing device a... First reagent dispensing position b... Second reagent dispensing position 5. ...Reagent bottle 6...Bottle holder 7...
・Holder rotation control device

Claims (1)

[Claims] A plurality of reagent bottles containing a first reagent or a second reagent corresponding to a measurement item, a turret-shaped bottle holder holding the reagent bottles, and a reagent corresponding to the measurement item by controlling rotation of the bottle holder. A holder rotation control device that transports a reagent bottle containing a reagent to a first reagent dispensing position and a second reagent dispensing position, and a holder rotation control device that aspirates a required amount of the first reagent in the reagent bottle that has arrived at the first reagent dispensing position. a first reagent dispensing device for dispensing the second reagent into the reaction container; A reagent dispensing device consisting of: