JPS61249383A - Culture tool - Google Patents

Abstract

PURPOSE:A sealed container containing an isotonic solution in the interior is connected to a culture container which has a granular or powder medium in the interior and is kept in a reduced pressure state by a connecting tube equipped with communicating pieces, a test specimen is inoculated into the isotonic solution, blended with it and distributed to the culture containers in the closed state. CONSTITUTION:A test specimen is injected into the container 1, inoculated into the isotonic solution 2, blended with it, the head parts of the small pieces of the communicating pieces 16 are folded and the connecting tube 25 is opened. The blended solution in the container 1 is divided into approximately equal amounts in the containers 7 and 8. The containers 7 and 8 contain the powder and/or granular mediums 9 and 10 and are kept in a reduced pressure. The clamps 17 and 18 are pressed to close the tube 15 and the ventilation needle 19 is pierced into the thin part 12a of the rubber stopper of the aerobic culture container 8 to make the interior of the container 8 at normal pressure.

Description

DETAILED DESCRIPTION OF THE INVENTION (Field of Industrial Application) The present invention relates to a culture instrument used for bacterial testing of blood and the like.

(Prior Art and its Problems) In cases of bacteremia, sepsis, etc., it is necessary to quickly detect pathogenic bacteria from blood culture, and various blood culture instruments used for this purpose have been proposed and marketed.

Generally, blood collected from a patient is immediately inoculated into a culture bottle or culture tube containing an enrichment medium and cultured to check for bacterial growth. Since there are aerobic bacteria and anaerobic bacteria, conventionally, samples were inoculated into culture media in separate containers and cultured under conditions depending on the bacterial species.

However, this method involves inoculating blood into aerobic and anaerobic media, which is not only cumbersome to operate, but also increases the chance of the blood sample being exposed to the outside air, increasing the possibility of sample contamination. becomes higher. Further, there was a problem in that the necessary information had to be entered on the label for each container. Furthermore, since conventional culture media are stored in a liquid or semi-liquid state, there is also the problem that deterioration and deterioration of the medium over time cannot be avoided.

The present invention was proposed as a result of studies in order to solve the above-mentioned problems.

(Means for Solving the Problems) As shown in FIGS. 1 and 2 corresponding to embodiments, the present invention comprises a container l in which an isotonic wire 2 is sealed, and a powder and/or Alternatively, it consists of a culture container 7.8 containing a granular culture medium 9° 10 and maintained under reduced pressure. 16 are provided. In the example, the culture container is an anaerobic culture container! ! 7 and an aerobic culture container 8.

(Function) After injecting a sample such as blood or cerebrospinal fluid into the container 1 containing the isotonic tube 2, the communication pieces 16, 16 are broken and the mixed solution of the isotonic tube and the sample is distributed to each container 7.8. However, in the aerobic culture container 7, the specimen in the inner container 7.8 is cultured while maintaining air permeability.

(Example) Examples of the present invention will be described below with reference to the accompanying drawings.
First, in FIG. 1, 1 is a container in which isotonic fluid 2 such as physiological saline is sealed, and a space 3 of the container 1 is filled with N.
Inert gas such as 2 gas is sealed.

A rubber stopper 4 is attached to the upper opening of the container l with a cap 5, and an introduction pipe 6 is inserted that passes through the rubber stopper 4 and extends near the bottom.

Further, 7 is a container for anaerobic culture, 8 is a container for aerobic culture,
Each container contains a powdered and/or granular medium 9. lO
is sealed, and the inside of each container is maintained at a reduced pressure state. Said medium 9. 1O is a dried culture medium with normal components and made into powder and/or granule form. In this example, different culture medium components are used for aerobic and anaerobic bacteria.

Furthermore, rubber stoppers 11.12 are also attached to the upper end openings of these containers 7.8 by caps 13.14, respectively.

These containers l, 7. and 8 are connected by a connecting tube 15 made of soft synthetic resin such as vinyl chloride. That is, one end of the connecting tube 15 is connected to the upper end of the introduction pipe 6 of the anaerobic culture container 1, and the other end is bifurcated, and one end penetrates the rubber stopper 11 of the anaerobic culture container 7. At the same time, the other end is penetrated by the rubber stopper 11 of the aerobic culture container 8. Furthermore, communication pieces 18 and 16 are enclosed in the branch tubes of the connection tube 15, respectively.

FIG. 2 shows an example of this communication piece 16, in which a large diameter tube 20 is installed between the connecting tubes 15a and 15b.
into the large diameter tube 20, and connect the hard resin communication tube 2 to the large diameter tube 20.
1 is enclosed. This communication tube 21 has a lower end open and an upper end sealed by a small head 22, and when the small head 22 is pressed from the outside of the large diameter tube 20, it will break at the thin wall portion 23. .

In addition, in FIG. 1, 17 and 18 are clamps, and 19 is a ventilation needle.

The above-mentioned culture equipment is preferably sterilized by γ-ray irradiation. In this case, the materials of the containers 1.7 and 8 are preferably polyvinyl chloride, ethylene-vinyl acetate, etc., and the rubber stoppers 4, 11.
The material 12 is made of natural rubber, urethane, butadiene, etc., and the caps 5, 13, and 14 are of any type as long as they are hard plastics that can withstand sterilization conditions and do not deteriorate.

Next, an example of the use of the present invention will be described. First, blood, cerebrospinal fluid, etc. are collected from a patient using a blood sampler or the like, and the sample is injected into a container 1 and mixed in an isotonic tube 2. The sample is injected by inserting an injection needle into the thin wall portion 4a of the rubber stopper 4.

Thereafter, the small heads 22 of the communicating pieces 16, 16 are broken to open the connecting tube 15, and the liquid mixture in the container 1 is distributed in approximately equal amounts to the containers 7 and 8. In this case, containers 7, 8
Since the is under reduced pressure, the mixed liquid can be easily transferred.

Subsequently, the clamps 17 and 18 are pushed in to close the connecting tube 15, and the aeration needle 19 is inserted into the thin rubber stopper portion 12a of the aerobic culture container 8 to bring the inside of the container 8 from depressurized to normal pressure.

Next, remove the cap 14 of the aerobic culture container 8, and
2 to create a gap, then lightly tighten the cap 14 to ventilate the cap 5 of the anaerobic culture container 7.
This is done to release the internal pressure that increases due to gas production by anaerobic bacteria.

In this state, under predetermined conditions (for example, 35 to 37
℃, for 7 to 14 days).

(Effects) According to the present invention described above, a container in which a manufacturing method is sealed and a culture container in which a powdered and/or granular medium is contained and which is maintained in a reduced pressure state are connected. They are connected by tubes, and a communicating piece is provided in the middle of this connecting tube, so after inoculating and mixing the specimen according to the same manufacturing method, by opening the communicating piece, the mixture can be transferred to the anaerobic culture container in a sealed state. and an aerobic culture container. Therefore, the operation is simpler than in the past, and there are fewer opportunities for the sample to come into contact with the outside air, which greatly reduces the possibility of contamination.

In addition, since the anaerobic culture container and the aerobic culture container are integrated via a connecting tube, it is only necessary to fill in the necessary information on the label once, and storage can be ensured. Furthermore, according to the present invention, containers for anaerobic and aerobic culture7.
8 contains powdered and/or granular media, so there is less deterioration and deterioration compared to liquid media, etc., and there is almost no effect even after γ-ray sterilization. This is an extremely effective invention.

[Brief explanation of drawings]

FIG. 1 is a perspective view showing an embodiment of the present invention, and FIG. 2 is a sectional view showing an example of a communication piece. In the figure, 1 is an isotonic transfer container, 2 is an isometric manufacturing method, 7 is an anaerobic culture container, 8 is an aerobic culture container, 9.10 is a culture medium, 15 is a connecting tube, and 16 is a communication piece.

Claims (2)

[Claims] (1) It consists of a container filled with an isotonic solution inside, and a culture container filled with a powdered and/or granular medium inside and maintained in a vacuum state, and these containers are connected by a connecting tube. A culture device characterized in that the connecting tube is connected by a connecting tube, and a communicating piece is provided in the middle of the connecting tube. (2) The invention described in item 1 above, wherein the culture container consists of an anaerobic culture container and an aerobic culture container.