JPS61240970A - Suppression of malignant tumor - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION Field of Industrial Application This invention relates to a method for suppressing malignant tumors in animals including humans in the medical field. Specifically, the present invention relates to a method for treating a tumor in which eosin yellow is administered into the body of an animal having a malignant tumor, and then the malignant tumor is irradiated with a laser beam to suppress or shrink the tumor.

BACKGROUND OF THE INVENTION Methods for treating malignant tumors occurring in animals including humans include surgical procedures such as surgical removal of the tumor and medical treatments using anti-cancer drugs.

As a surgical method, research has recently been conducted on a method of suppressing or degenerating malignant tumors through treatment using a combination of laser beam irradiation and photosensitizing drugs. Hematoporphyrin derivatives (hereinafter abbreviated as HpD) are often used as photosensitizing drugs. For example, there is the following literature regarding the treatment of lung cancer. “Yoshihiro Hayata, Japanese Clinical Journal, Volume 42, No. 1, 1
Pages 84-195, 1984. Problems to be Solved by the Invention The conditions required for a photosensitizing drug used in laser beam treatment of tumors are that the drug has a high affinity for tumors and that the drug is safe for living organisms. It can be used. )I
Although pD has a high affinity for tumors, side effects such as sunburn have been observed. Acridine orange also has a high affinity, but it is suspected of being carcinogenic and poses a safety problem.

The development of more useful photosensitizing drugs for tumor treatment with laser beams is desired.

Means for Solving the Problems As a result of conducting research on photosensitizing drugs, the present inventor discovered that eosin yellow has a high affinity for tumors. Eosin yellow is a fluorescent substance, and by observing the fluorescence emitted by eosin yellow, which has a strong affinity for tumor cells and tumor tissues, malignant tumors can be distinguished from other tissues and can be observed, making it extremely useful for diagnosis. A patent application was filed for this as a cancer diagnostic reagent (patent application filed in 1983).
-27224). As a result of further research, the inventor found that
We have found that eosin yellow is also useful as a photosensitizing agent for laser treatment of tumors. Eosin yellow is administered intravenously to an animal, and after an appropriate period of time, a laser beam of a specific wavelength is applied to the animal. By irradiating the tumor into the tumor, tumor suppression or regression was observed, and it was effective in treating malignant tumors.

Eosin yellow has been used in foods as food coloring No. 103, is highly safe, and is suitable as a photosensitizer.

Hereinafter, the subject matter and effects 1 and 7 of the present invention will be described in detail with reference to Examples.

Example 7 Using 7-week-old Tonryu female rats, 6 x 10' ascites-type hepatoma (AH-130) cells were subcutaneously transplanted into the dorsal region of each rat, and AI (-130) cells were subcutaneously transplanted to a size of 20 to 30 mm. Rats were used in the study.

500 mg of Eosin Yellow (special grade reagent manufactured by Tokyo Kasei) was dissolved in 10o+1 physiological saline and administered to the above rats via the tail vein at a rate of 100 mg/kg per rat.

Six hours after administration of eosin yellow, wavelength 514.5
ns, argon laser with output 200mW (model, NE
A 1 mmφ needle guided with CGLG3300) was inserted into the tumor mass, irradiated for 3 minutes, and the major and minor axes of the tumor mass were orally measured.

As a control example, using the above-mentioned rats, experiments were conducted on a group in which neither eosin yellow administration nor laser beam irradiation was performed, and a group in which only laser beam irradiation was performed.

The weight of the tumor mass (g
) was calculated. This formula is also used in Japan to determine the effectiveness of antitumor drugs (for example, Masaaki Iigo, J. Pha
rm, Dyn, Volume 1, Pages 49-54, 1978
Year). To facilitate efficacy evaluation, the weight of the tumor mass before eosin yellow administration and laser beam irradiation was
The tumor growth rate was calculated as follows.

The results thus obtained are summarized in Table 1.

In addition, the results of the t-test of effectiveness are listed in Table 2.

As is clear from the assay results, the laser alone has anti-1L
ffi tumor effect was observed, but it was found that combined use with eosin yellow suppressed tumor growth even more significantly (p < 0.05).

Furthermore, the effect can be further enhanced by adjusting the dose of eosin yellow, the amount of laser beam irradiation, and the timing of the combined use of both.

Effects of the Invention The tumor suppression method using a combination of highly safe eosin yellow and laser beams significantly suppresses tumor growth as shown in the examples, and the present invention has very high utility value in the medical field. be.

Claims (1)

[Claims] A method for suppressing a malignant tumor, which comprises administering eosin yellow into the body of an animal having a malignant tumor, and then irradiating the tumor site with a laser beam.