JPS61227787A - Deodorization of fermentation product - Google Patents
Deodorization of fermentation productInfo
- Publication number
- JPS61227787A JPS61227787A JP60068527A JP6852785A JPS61227787A JP S61227787 A JPS61227787 A JP S61227787A JP 60068527 A JP60068527 A JP 60068527A JP 6852785 A JP6852785 A JP 6852785A JP S61227787 A JPS61227787 A JP S61227787A
- Authority
- JP
- Japan
- Prior art keywords
- carbon dioxide
- autoclave
- dioxide gas
- valve
- fermentation product
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Abstract
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は発酵生産物の脱臭方法Ｅこ関する。[Detailed description of the invention] [Industrial application field] The present invention relates to a method for deodorizing fermentation products.
発酵法を利用して得られる酵素、薬品、食品等の発酵生
産物は発酵に伴う独特の臭気を有している。従って多（
の場合、使用者に不快感をもたらすことのない程度まで
臭気成分を除去する必要がある。Fermented products such as enzymes, drugs, and foods obtained using fermentation methods have a unique odor associated with fermentation. Therefore, many (
In this case, it is necessary to remove odor components to the extent that they do not cause discomfort to the user.
従来、発酵生産物の臭気成分の除去方法としては、吸着
法、クロマト分離法、イオン交換樹脂法、硫酸アンモニ
ウム等による塩析法、等電沈澱法、アルコール等による
溶媒沈澱法、溶媒抽出法等が知られている。しかしなが
ら、これらの方法はいずれも臭気成分の除去Ｉこ際して
発酵生産物それ自体の損失を伴い、その分だけ製品の製
造原価を高めていた。Conventionally, methods for removing odor components from fermented products include adsorption methods, chromatographic separation methods, ion exchange resin methods, salting out methods using ammonium sulfate, isoelectric precipitation methods, solvent precipitation methods using alcohols, etc., and solvent extraction methods. Are known. However, all of these methods involve the loss of the fermentation product itself during the removal of odor components, increasing the manufacturing cost of the product accordingly.
一例として、発酵法によるプロテアーゼをエタノールを
使用した溶媒沈澱法によって脱臭する場合を示せば、人
間に不快感を与えない程度まで発酵臭を減少させると溶
解平衡関係から全プロテアーゼの約３０ｃｓがエタノー
ル側に溶解して持ちされてしまう。これに実プロセスで
の損失を含めると損失率は更に大きな値となる。As an example, if protease produced by a fermentation method is deodorized by a solvent precipitation method using ethanol, if the fermentation odor is reduced to a level that does not cause discomfort to humans, approximately 30 cs of the total protease will be absorbed by the ethanol based on the solubility equilibrium relationship. It will be dissolved and carried away. If losses in the actual process are included, the loss rate becomes even larger.
本発明の目的は、脱臭に伴う製品損失を伴わない発酵生
産物の脱臭方法を提供することにある。An object of the present invention is to provide a method for deodorizing fermented products that does not involve product loss due to deodorization.
〔問題点を解決するための手段及び作用〕本発明者らは
良品損失を伴わない発酵生産物の脱臭法について鋭意検
討を進めてきた結果、炭酸ガス抽出法によれば酵素失活
等の製品損失や品質の変化を伴わずｌこ脱臭可能である
ことを見出し、本発明を完成した。[Means and effects for solving the problem] The present inventors have conducted intensive studies on a method for deodorizing fermented products that does not involve loss of good quality products. The present invention was completed based on the discovery that deodorization is possible without loss or change in quality.
すなわち、本発明は発酵生産物を炭酸ガスで処理して臭
気成分を抽出除去することを特徴とする発酵生産物の脱
臭方法である。That is, the present invention is a method for deodorizing fermented products, which is characterized by treating the fermented products with carbon dioxide gas to extract and remove odor components.
以下、添付図面を参照しながら本発明方法の詳細な説明
をする。Hereinafter, the method of the present invention will be described in detail with reference to the accompanying drawings.
第１図は本発明の脱臭方法を実施する装置例の概略図で
ある。FIG. 1 is a schematic diagram of an example of an apparatus for carrying out the deodorizing method of the present invention.
脱臭処理を行う発酵生産物（中間製品、または造粒、オ
ーバーコート等を施した最終製品）と吸着剤（活性アル
ミナ、活性炭等）をそれぞれガーゼ等に包んでオートク
レーブ４中に投入した後、弁２を開いてボンベｌより液
化炭酸ガスを供給する。次いで弁２を閉じ、弁３を開い
て、オートクレーブ４中の空気および炭酸ガスを放出す
る。このような炭酸ガスの供給と放出の操作を繰返して
、オートクレーブ４中の残留空気を除去すると共に、液
化炭酸ガスの蒸発潜熱によってオートクレーブを冷却シ
て、オートクレーブ内に液化炭酸ガスを充填する。この
操作の終点は弁３を開いた時に排気口から雪、状のドラ
イアイス片が吹き出しはじめることにより確認でき、通
常５〜６回の操作で充填できる。After wrapping the fermentation product to be deodorized (intermediate product or final product after granulation, overcoating, etc.) and adsorbent (activated alumina, activated carbon, etc.) in gauze, etc., and putting it into autoclave 4, Open 2 and supply liquefied carbon dioxide from cylinder 1. Then, valve 2 is closed and valve 3 is opened to release the air and carbon dioxide gas in autoclave 4. By repeating this operation of supplying and discharging carbon dioxide, residual air in the autoclave 4 is removed, the autoclave is cooled by the latent heat of vaporization of the liquefied carbon dioxide, and the autoclave is filled with liquefied carbon dioxide. The end point of this operation can be confirmed by the fact that when the valve 3 is opened, snow-shaped pieces of dry ice begin to blow out from the exhaust port, and filling can usually be completed in 5 to 6 operations.
その抜弁３を閉じ、弁２を開いてオートクレーブ内の圧
力が所定の値に達した抜弁２を閉じる。The vent valve 3 is closed, the valve 2 is opened, and the vent valve 2 is closed when the pressure inside the autoclave reaches a predetermined value.
このようにしてオートクレーブ内に発酵生産物、吸着剤
および液化炭酸ガスを封じ込めた後、熱媒タンク５内に
熱媒６を供給し、加熱ヒーター７によって、通常炭酸ガ
スの臨界温度（３１，３℃）以上に熱媒を加熱する。加
熱開始と共にオ・−ト。After sealing the fermentation product, adsorbent, and liquefied carbon dioxide in the autoclave in this way, a heating medium 6 is supplied into the heating medium tank 5, and the heating medium 6 is heated to the critical temperature of carbon dioxide (31,3 ℃) or above. Auto when heating starts.
クレープ内の温度および圧力はほぼ炭酸ガスのモリエ線
図上の等比容積線に添って上昇する。The temperature and pressure inside the crepe rise approximately along the isovolume line on the Mollier diagram of carbon dioxide gas.
オー、トクレープ内の圧力および温度が所定の値に達し
た後、オートクレーブ内の圧力および温度を一定に保つ
ように熱媒ヒーターを調整しながら、所定時間放置した
後、弁【３ノを開いて炭酸ガスを排気すると、オートク
レーブ中に全（無臭となった製品を得ることができる。After the pressure and temperature inside the autoclave reach a predetermined value, adjust the heating medium heater to keep the pressure and temperature constant in the autoclave, and leave the autoclave for a predetermined period of time, then open valve [3]. When the carbon dioxide gas is evacuated, a completely odorless product can be obtained in the autoclave.
本発明の方法では超臨界状態の炭酸ガスで抽出すると脱
臭速度が早いので好ましいが、脱臭する対象物蒼こよっ
ては超臨界状態未満の液化炭酸ガスによる脱臭も可能で
ある。すなわち、炭酸ガスによる抽出処理温度は、一般
に臨界温度以下の２５℃程度から、発酵生産物が熱的に
変質しない９０℃程度までの温度が採用される。In the method of the present invention, it is preferable to extract with carbon dioxide gas in a supercritical state because the deodorization rate is fast, but depending on the nature of the object to be deodorized, deodorization with liquefied carbon dioxide gas in a less than supercritical state is also possible. That is, the temperature for extraction treatment with carbon dioxide gas is generally from about 25° C., which is below the critical temperature, to about 90° C., at which the fermentation product is not thermally altered.
また圧力は６０〜３００Ｋｅ／ｃｊｃの範囲が好ましく
、抽出処理時間は２時間〜１０時間程度必要である。Further, the pressure is preferably in the range of 60 to 300 Ke/cjc, and the extraction treatment time is required to be about 2 to 10 hours.
なお、上記の第１図の例は、オートクレーブを利用した
パッチ式脱臭についてのものであり。The example shown in FIG. 1 above is for patch deodorization using an autoclave.
脱臭対象物と共に吸着剤を共存させて処理しているが、
吸着剤は必ずしも用いる必要はな（。The deodorizing target is treated with an adsorbent coexisting with it, but
It is not necessary to use an adsorbent (.
臭気成分の全てを炭酸ガスと共に排気してもよいＯ
本発明の方法は、上述のバッチ式だけでな（、炭酸ガス
を圧縮機を利用して循環し、抽出器を出た炭酸ガスを、
（１）活性アルミナ、活性炭等を充填した吸着塔に通し
脱臭するか、（２）減圧、加熱または冷却した後臭気成
分分離槽−こ通すかのいずれかの処理を行った後、炭酸
ガスを再び圧縮して抽出器に戻すようなプロセスも可能
である。All of the odor components may be exhausted together with the carbon dioxide gas.
After either (1) deodorizing by passing through an adsorption tower filled with activated alumina, activated carbon, etc., or (2) passing through an odor component separation tank after being depressurized, heated, or cooled, carbon dioxide gas is removed. Processes such as recompressing and returning to the extractor are also possible.
本発明の脱臭方法は、
（１） 臭気成分のみを除去しつる画期的方法であり
、発酵生産物製品の損失、失活等が殆どないこと、
（２）比較的低温度で処理できるため製品が熱的に劣化
しないこと、
Ｏ） 炭酸ガスの透過性が高いため、精製、造粒等の最
終加工を施す前の中間製品に対して適用可能であるのみ
ならず、造粒、オーバーコート等を施した最終製品に対
しても適用可能であること、
（４）炭酸ガスを使用するため製品中の残留毒性の心配
がないこと、
（５） 処理ｌこ伴う、製品の粉化や破砕等の損傷が
殆どないこと、
（６） 既存の発酵生産物製造プロセスに何らの変更
を加えることなく、脱臭を行うことができること等の特
長を有する。The deodorizing method of the present invention is: (1) It is an innovative method that removes only odor components, and there is almost no loss or deactivation of fermented products; (2) It can be processed at a relatively low temperature. O) Because the product does not deteriorate thermally, it has high permeability to carbon dioxide gas, so it can be applied not only to intermediate products before final processing such as refining and granulation, but also to granulation and overcoating. (4) Since carbon dioxide gas is used, there is no concern about residual toxicity in the product. (5) There is no need to worry about the pulverization or crushing of the product as a result of the treatment. (6) Deodorization can be carried out without making any changes to the existing fermentation product manufacturing process.
実施例１
発酵法で生産したプロテアーゼの造粒品３０？および活
性アルミナ（水沢化学■製、ネオビードロ）６０９）ｆ
、−、それぞれガーゼに包んで、５００ｃｃのオートク
レーブに入れ、液化炭酸ガスの供給と放出を繰返して、
液化炭酸ガスを充填し、圧力２２０Ｋｆ／ｄ Ｇ 、温
度６０℃に７時間保持して抽出を行った。その結果、強
い発酵臭を発していた原製品は全く臭気を検知しえない
レベルｌこまで脱臭された。Example 1 Granulated product of protease produced by fermentation method 30? and activated alumina (manufactured by Mizusawa Kagaku ■, Neo Vidro) 609) f
,-, each was wrapped in gauze and placed in a 500cc autoclave, and the supply and release of liquefied carbon dioxide gas was repeated.
The chamber was filled with liquefied carbon dioxide gas and kept at a pressure of 220 Kf/d G and a temperature of 60° C. for 7 hours for extraction. As a result, the original product, which had emitted a strong fermented odor, was deodorized to a level where the odor could not be detected at all.
臭気の指標として、アミン臭を代表する揮発性窒素につ
いて、揮発性窒素分析法によりホウ酸吸収を利用し窒素
量を測定したところ、原製品中に０．２２重量％存在し
ていた揮発性窒素原子は０．００３重量％まで減少して
いた。As an odor indicator, we measured the amount of volatile nitrogen, which is representative of amine odor, using boric acid absorption using a volatile nitrogen analysis method, and found that 0.22% by weight of volatile nitrogen was present in the original product. The atoms were reduced to 0.003% by weight.
実施例２
発酵法で生産したα−アミラーゼの中間製品（造粒前の
粗酵素）５０ｔおよび吸着剤として実施例１と同じ活性
アルミナＳＯｔを５００ ｃｃのオートクレーブに投入
し、実施例１と同様にして２１０Ｋｔ／ｊＧ、温度２８
℃に８時間保持して抽出を行った。その結果、原粗酵素
中に０．１７重量％存在していた揮発性窒素原子は、脱
臭処理後０．００２重量幅まで減少し、全く無臭の状態
となった。Example 2 50 t of an intermediate product of α-amylase (crude enzyme before granulation) produced by the fermentation method and the same activated alumina SOt as in Example 1 as an adsorbent were placed in a 500 cc autoclave, and the same procedure as in Example 1 was carried out. 210Kt/jG, temperature 28
Extraction was carried out by holding at ℃ for 8 hours. As a result, the volatile nitrogen atoms present in the original crude enzyme at 0.17% by weight decreased to 0.002% by weight after the deodorization treatment, resulting in a completely odorless state.
第１図は本発明の脱臭方法を実施する装置例の概略図で
ある。
図中符号：
１・・・液化炭酸ガスボンベ；２，３・・・弁：４・・
・オートクレーブ；５・・・熱媒タンク：６・・・熱媒
；７・・・加熱ヒーター：８・・・発酵生産物および吸
着剤。FIG. 1 is a schematic diagram of an example of an apparatus for carrying out the deodorizing method of the present invention. Codes in the diagram: 1...Liquid carbon dioxide cylinder; 2, 3...Valve: 4...
- Autoclave; 5... Heat medium tank: 6... Heat medium; 7... Heater: 8... Fermentation product and adsorbent.
Claims (1)
ることを特徴とする発酵生産物の脱臭方法。A method for deodorizing fermented products, characterized by treating the fermented products with carbon dioxide gas to extract and remove odor components.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60068527A JPS61227787A (en) | 1985-04-02 | 1985-04-02 | Deodorization of fermentation product |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60068527A JPS61227787A (en) | 1985-04-02 | 1985-04-02 | Deodorization of fermentation product |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS61227787A true JPS61227787A (en) | 1986-10-09 |
Family
ID=13376285
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60068527A Pending JPS61227787A (en) | 1985-04-02 | 1985-04-02 | Deodorization of fermentation product |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61227787A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6387977A (en) * | 1986-10-01 | 1988-04-19 | Kinjirushi Wasabi Kk | Separation of active component from cruciferous plant |
| JPH04222576A (en) * | 1990-12-21 | 1992-08-12 | Shokuhin Sangyo High Separeeshiyon Syst Gijutsu Kenkyu Kumiai | Preparation of powdery food |
| JP2016191028A (en) * | 2015-03-31 | 2016-11-10 | 理想科学工業株式会社 | Method for producing oily ink for inkjet |
-
1985
- 1985-04-02 JP JP60068527A patent/JPS61227787A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6387977A (en) * | 1986-10-01 | 1988-04-19 | Kinjirushi Wasabi Kk | Separation of active component from cruciferous plant |
| JP2534043B2 (en) * | 1986-10-01 | 1996-09-11 | 金印わさび株式会社 | Method for separating active ingredients from cruciferous plants |
| JPH04222576A (en) * | 1990-12-21 | 1992-08-12 | Shokuhin Sangyo High Separeeshiyon Syst Gijutsu Kenkyu Kumiai | Preparation of powdery food |
| JP2016191028A (en) * | 2015-03-31 | 2016-11-10 | 理想科学工業株式会社 | Method for producing oily ink for inkjet |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2774491C (en) | Freeze drying system | |
| JP3931084B2 (en) | Tobacco treatment method | |
| EP0616821A1 (en) | Fragrance extraction | |
| GB2031570A (en) | Method for drying water-containing substances | |
| US4511508A (en) | Process for drying of natural extracts prepared by high pressure extraction | |
| GB2044596B (en) | Method and apparatus for expanding tobacco | |
| WO1999026667A3 (en) | Steam sterilisation or steam disinfecting method and device for carrying out the method | |
| JPS61227787A (en) | Deodorization of fermentation product | |
| JP2016537025A (en) | Method and apparatus for processing raw materials and effectively producing meat and sausage-based raw salted products | |
| CA1222922A (en) | Expansion of tobacco | |
| JPH09505228A (en) | Sterilization and drying method and device | |
| EP1503854B1 (en) | Method for drying a product using a regenerative adsorbent | |
| USRE20969E (en) | Ltophiuc biologically active sub | |
| EP0896794B1 (en) | Method and apparatus for roasting and cooling beans | |
| US1630985A (en) | Means for preparing products of organic origin | |
| US3341335A (en) | Method of sterilizing dry particulate material | |
| US877698A (en) | Treatment of tobacco. | |
| JPH02292216A (en) | Removal of solvent of drugs by extraction with supercritical carbon dioxide gas | |
| JPS6236178A (en) | Extraction and separation of organic component from grape cluster brewing residue | |
| JPH07115898A (en) | Apparatus for removing harmful substance with active carbon | |
| JPH0243522B2 (en) | ||
| Samonin et al. | Variation of sorption properties of fullerene black in storage | |
| CN105078907B (en) | The freeze-drying method of hydrochloride for injection ligustrazine lyophilized preparation | |
| WO1998037777A3 (en) | Method and device for preserving capillary porous products, especially baked products | |
| WO2006077656A1 (en) | Method of low-temperature drying of substance under vacuum and apparatus therefor |