JPS61224984A - Multiplying agent for bacterium belonging to genus bifidobacterium - Google Patents

Abstract

PURPOSE:To promote extremly multiplication of a bacterium belonging to the genus Bifidobacterium, by adding a specific N-acyllactam compound to a medium. CONSTITUTION:An N-acyllactam compound shown by the formula (R is -CH2-, CH2-CH2-, or -CH+CH-; n is 3-5) is used as a multiplying agent for a bacterium belonging to the genus Bifidobacterium. 1-(3-Phenylpropanoyl)-2-piperidone, 1-cinnamoyl-epsilon-caprolactam, etc. may be cited as the compound. When the bacterium belonging to the genus Bifidobacterium is inoculated to BL agar medium containing about 10mg of the multiplying agent, 1.2-6 times as much the bacterium as that in a medium not containing it is multiplied.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a growth layer of Bifidobacterium bacteria.

Bifidobacterium, a member of the genus Bifidobacterium, was well known as a bacterium that exists in almost pure culture in the intestines of breast-fed infants, but today Bifidobacterium plays an important role in human health. The reason why this bacterium has been in the spotlight is because it has been confirmed that this bacterium is present as one of the dominant routes in the intestines of adults when they are healthy. , it is clear that it decreases or disappears in cases such as aging, so recently,
Many medicines and food products using this bacterium are now on the market, and live bacteria preparations are used to prevent and treat diarrhea and enteritis, which are difficult to treat in infants that are considered impossible to treat with drugs. It is also said to be effective for sexual hypoxia, and its relationship with anti-cancer effects is also being studied.

In view of these circumstances, research on propagating bifidobacteria has become active, and methods using lactulose and flynose have been proposed in the past, and in recent years, methods using fructooligosaccharides have also been proposed. However, these propagation agents are expensive and have problems in their proliferative effectiveness.

Therefore, the present inventors conducted various studies on growth agents for Bifidobacterium bacteria, and discovered that an inexpensive known product obtained by a synthetic method has an excellent growth effect.
The present invention was completed based on this knowledge.

Already, the present invention has the following features: (However, R is -CH, -, -CH* -OH, +.

or -CH-CH-, n-3 ~5) Bifidobacterium, which consists of Cuctum compounds Concerning a propagating agent for Therium genus #l+1.

The propagation agent of the present invention can be produced using well-known methods (11 Reaction of lactam and acid anhydride (2) Direct reaction of lactam and acid chloride (3)
) Reaction of lactam and acid chloride in the presence of 5m amines or dimethylformamide (4) Reaction of alkali metal salt of lactam and acid chloride (5) Reaction of lafuchim nityl and acid chloride (6) Trimethyl μ It can be easily produced by ff reaction with an acid chloride via a silicate fuctam (7) Reaction of an intermediate with an acid using a 2-chloropyridinium salt and a lactam.

For example, to produce lactam and acid chloride by direct reaction method, phenylacetic acid, phenylgropionic acid, and cinnamic acid are produced by thionyl chloride and phosphorus pentachloride.

Phosphorus trichloride9. After acid chloridation with oxalyl chloride etc., 2-pyrrolidone and 2-piperidone.

By reacting with E-caglolactam, R in the above general formula can be changed to -OH, -, -CH-CH+,
N-acyllactam compounds of -OH, -CH, - can be easily produced.

When using the proliferating agent of the present invention, it can be used in any desired form, such as a solid form or a powder form, depending on the purpose of use, and a desired amount can be added to foods and drinks. Furthermore, tablets, fermentation tubes, powders, tablets, etc. containing bifidobacteria may be added and used.

Examples of the present invention will be further explained below.

Production Example 5 - Add thionyl chloride tvsy to 1 g of phenylpropionic acid, hold at 80°C for 5 hours, and remove generated gas and residual thionyl chloride under reduced pressure.

To this reaction mixture, a solution containing 0.7y of 5-anhydrous pyridine IC2-piperidone was added and maintained at 80 to 90°C for 5 hours, ether was added to the reaction mixture, and the organic solvent layer was separated. This was washed successively with an IN hydrochloric acid solution, an IJ solution, and water, and after dehydration with sodium sulfate, the solvent was distilled off. The residue was dissolved in a mixed solvent of ethyl acetate and n-hexane and crystallized to give +-(5-phenylpropanoyl N).
-2-Hyperitone L86F was obtained.

Example 1 The growth agents shown in Table 1 were added to a BL agar medium (Tsui) +1!
Using a test medium to which 10 capes were added per sample, the influence on the growth of various Bifidobacterium bacteria was measured under anaerobic conditions at 35°C for 48 hours. The strains used are shown in Table 2, and the results are shown in Table 6.

Table 1 Test Samples Table 2 Table 3 Example 2 Adult male feces (55 years old) was used as a sample, and 1-(2-7 enyl ethanoyl Iv) -2- ? The effects of Peritone and 5μ2β (medium volume) were measured on the total number of bacteria and differences in flora.
Table 4 shows the total number of bacteria and the number of Bifidobacterium bacteria.

Table 4 Example 3 The same test as Example 2 was carried out using
I/)-2-pyrrolidone was used and newborn feces were used as the specimen. Table 5 shows the total number of Bifidobacterium bacteria.

Table 5

Claims (1)

[Claims] General formula ▲ Numerical formula, chemical formula, table, etc. ▼ (However, R is -CH_2-, -CH_3-CH_2-,
or -CH═CH-, and n=3 to 5), a growth agent for Bifidobacterium bacteria.