JPS6122252A - Paracort analyser - Google Patents

Abstract

PURPOSE:To simply and rapidly analyze paracort in serum by, eluting paracort in serum by a high performance liquid chromatograph and converting said paracort to a paracort radical before detecting the same. CONSTITUTION:When an apparatus is operated to inject a serum specimen preliminarily subjected to deproteinizing treatment in a column 1, paracort contained in the specimen is separated from other impurities and discharged to a mixing coil as a paracort ion while the paracort ion receives the action of sodium hydrosulfide from a specimen tank 5 to form a paracort radical. Thus formed paracort radical is specifically detected by a visible light absorptiometer 7 and a signal with a level proportional to the concn. thereof is outputted.

Description

DETAILED DESCRIPTION OF THE INVENTION A. Field of Industrial Application The present invention relates to an apparatus for quantitatively analyzing paraquat present in serum.

Oral, the prior art herbicide paraco-1 (Methyl violo
gen) causes toxic symptoms in humans if misused or swallowed, so it is necessary to measure the concentration of paraquat in the blood during the treatment process.

To analyze paraquat in blood, add a tricloacetic acid solution to the blood sample, centrifuge at 500G for 15 minutes, remove the supernatant, add tricloacetic acid solution to the precipitate, and centrifuge at 500G for 5 minutes. Perform centrifugation to remove the supernatant, mix both supernatants, concentrate and extract paraquat ions using an ion exchange resin to prepare a sample, collect this in a reaction tube, and then perform colorimetric determination. It was done by doing.

However, since an ion exchange resin is used, there are problems in that it not only takes time to concentrate and extract the sample, but also that the work process is complicated.

C. Objective: In view of the above-mentioned problems, the present invention provides a novel analyzer that can easily and quickly analyze paraquat in serum.

2. Structure, that is, the feature of the present invention is that paraquat in serum is eluted by high-performance liquid chromatography, and this is converted into paraquat radicals for detection.

E. Embodiments The details of the present invention will be explained below based on illustrated embodiments.

FIG. 1 shows an embodiment of the present invention, and the reference numeral l in the figure indicates a pipe whose minus side is connected to a mobile phase solvent tank 3 via a pump 2, and whose other side is a corrosion-resistant pipe made of stainless steel, fluororesin, etc. a high-speed liquid column communicating with a mixing coil 4 consisting of a
is 0.5 to 2 wt.
Sodium hydrosulfide (Na2S,
04) and a caustic soda aqueous solution. 7 is
The absorbance photometer is connected to the outlet of the mixing coil 4 and detects visible light of 600 nm or 900 nm, and is configured to output a detection signal to a display meter 8 such as a printer. In the figure, reference numeral 9 indicates a solution tank that receives the sample discharged from the visible light absorption photometer, and 10 indicates a sample injection port.

In the device configured in this way, when the device is operated and a serum sample that has been previously subjected to protein removal treatment is injected into column L,
The paraquat contained in the sample is separated from other impurities and discharged as paraquat ions to the mixing coil 4, and is then affected by the hydrosulfide storium from the reagent tank 5. Paraquat radical is produced by the reaction of Lacote radical.

The thus produced baraquatradil is specifically detected by the visible light absorption photometer 7, which outputs a signal at a level proportional to its concentration.

(Example) When a paraquat standard solution prepared by diluting a paraquat standard solution with a saturated ammonium chloride solution was injected into a column and analyzed, an output as shown in FIG. 2 (a) was obtained.

On the other hand, 1-
Take out the VF6 liquid for one day, add trichloroacetic acid solution to the precipitate, centrifuge at 500G for 5 minutes, take out the clear liquid, and mix both two clear liquids to use the serum sample as a sample. When this was injected into a column and analyzed, a unimodal peak was obtained as shown in the figure (b), indicating high selectivity without being affected by other components in serum. It was found that paraquat can be analyzed using

In this example, sodium hydrosulfide and a sodium hydroxide solution are used as reagents, but the same effect can be obtained by using an aqueous solution of NaBI (4) and an ethanol solution.

As explained above, according to the present invention, paraquat contained in serum is separated and extracted by high-performance liquid chromatography, and is made to interact with a reducing agent in a conduit. Paraquat in blood can be analyzed with high selectivity and accuracy without being affected by foreign substances or oxygen in the air. Further, after the serum sample is injected, the analysis work proceeds according to the normal flow of high-performance liquid chromatography, so that the analysis work can be speeded up and simplified.

[Brief explanation of drawings]

Fig. 1 is a configuration diagram of a device constituting an embodiment of the present invention;
Figures (a) and (b) are chromatogram diagrams showing the results of analysis by the same apparatus. 1...High performance liquid column 3...Mobile phase solvent tank 4
...Mixing coil 50...Reagent tank 7...
visible light absorption photometer

Claims (1)

[Claims] a high-performance liquid chromatography means for eluting and separating paraquat ions from a serum sample; a reagent tank containing a reagent for radicalizing paraquat ions; a mixing means for mixing the reagent from the reagent tank with the sample solution discharged from the chromatogram means; and a means for detecting visible light absorbance of the solution from the mixing means.