JPS6120850A - Production of standard liquid for simultaneous calibration of electrode for measuring sodium ion, potassium ion and calcium ion and ph - Google Patents

Abstract

PURPOSE:To obtain stably a standard soln. having a highly accurate concn. by adding a prescribed amt. of HCl and tris(hydroxymethyl)aminomethane(Tris) to a mixture composed of a prescribed amt. each of NaCl, KCl and CaCO3. CONSTITUTION:The prescribed amt. each of NaCl, KCl and CaCO3 are put into a tank for prepn. and further a prescribed amt. of HCl is added thereto to complete the reaction CaCO3+2HCl CaCl2+H2O+CO2. Pure water is added thereto to prepare a prescribed amt. of a soln. mixture which is then thoroughly stirred to make uniform the concn. The prescribed amt. of Tris is dissolved in the pure water and is added under stirring into the tank. A surface active agent (for example, ''Toriton X-100'') is finally added thereto and the pure water is added to the soln. to make the entire amt. to the prescribed amt. The NaCl, KCl and CaCO3 which have less hygroscopicity are used as the reagent in the stage of adjusting the sodium ion, potassium ion and calcium ion to the prescribed concn. and therefore the stable and exact weighing is made possible.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Application] The present invention is directed to, for example, sodium ions (hereinafter referred to as N
a+), potassium ions (hereinafter referred to as K+), calcium ions (hereinafter referred to as Ca+), and p
The present invention relates to a method for producing a standard solution for simultaneous calibration of H measurement electrodes.

[Prior art] In an apparatus for measuring the activity or concentration and pH of Na% and Ca in blood, the measurement electrodes for each ion and the pH measurement electrode are periodically filled with a standard solution that shows a certain known value for the electrodes. It is necessary to calibrate by

Conventionally, standard solutions for calibrating Na+, K+, Ca+, and pH measurement electrodes separately are commercially available, but a standard solution that can simultaneously calibrate the corresponding measurement type i for the above four components has not been realized. There was only a control solution (standard solution of the present invention) for simultaneous adjustment of a sensitive electrode and a pH electrode (Japanese Patent Publication No. 56-35052).

[Purpose of the invention]

Therefore, the purpose of the present invention is to
The purpose of the present invention is to provide a new and useful method for producing a standard solution for simultaneous calibration of H measurement electrodes.

〔Example〕

The reagent used to prepare the standard solution is sodium chloride (hereinafter referred to as
(hereinafter referred to as NaC1), potassium chloride (hereinafter referred to as Me't')
Calcium carbonate (hereinafter referred to as Ca COB), tris(hydroxymethyl)aminomethane (hereinafter referred to as Tris), hydrochloric acid (hereinafter referred to as HCl)
t)), and Triton X- acting as a surfactant.
100 (trade name), and it is preferable to use commercially available special grade products for all of these reagents.

For NaCl%KCl%Caco8, 110'C
It is desirable to use a product that has been heated and dried for at least 6 hours and then allowed to cool in a desiccator. Also, HcI! Use a 1N solution whose factor has been determined in advance to three decimal places. Note that the pure water used during adjustment has a conductivity of 1×10
-7Ω-1a-1 or less ion-exchanged water.

The concentrations and pH values of the above reagents are shown in Table 1.

1. Both the surface standard solution (cleaning solution) and H standard solution are Triton X-100.
is 10 ppm.

Next, an example of the procedure for preparing the L standard solution and H standard solution will be explained along with numerical values. Note that the adjustment amount is 50/.

(1) L standard solution, cleaning solution NaCl! (m, w, 58.44.) 350.6
40±0.005p, KCl (m, w, 74.56
)14.912±0.001F? , Ca COB(m
, w, 100.09)'6.005±0.001 y is placed in the adjustment tank, and further l regulation I (Cl7(0,9
Add 95(f(1,005)1885±0.IWd!, CaCO3+2HC1-CaC12+H20+CO2
↑ Complete the reaction. Further, pure water is added to the tank to prepare a mixed solution with a total weight of 301, and this is sufficiently stirred to dissolve each of the above reagents to prepare a mixed solution with a uniform concentration.

And Tris (m, w, 121.14) 302.8
5±o, oosp is dissolved in approximately 21% pure water to make a Tris aqueous solution, and this Tris aqueous solution is added into the tank with stirring. The reason why Tris is once diluted with pure water in the above is that % Tris is alkaline and if added at a high concentration, it will react with the mixed solution in the tank and form a precipitate.

Finally, add 5,0-10% solution of Triton X-100 and add pure water to make the total volume 5 ol. At this time, since the solution becomes foamy very easily due to Triton X-100, it is necessary to pay sufficient attention to stirring and the like.

(II) H standard solution The procedure for preparing the H standard solution may be carried out according to the above-mentioned (I) L standard solution, but the mixing amounts of each reagent are as follows. That is, NaClCm, w, 58.44), , 584.
400±0.005yKCI (m, w, 74.56),
, , , , , , , , 26.469±0.005yC
aCOa (m, w, 100.09), , 12.76
2±0.001 y1 regulation H(J(0,995(f(1
,005),,, 232510.1+++eTri
s (m, w, 121.14) , , 3
02.85±o, oo5y bird) 7X-100 (10%
solution), , , , , , , , , , , '5.0d It is desirable that each standard solution prepared as described above exhibits the accuracy shown in Table 2.

Table 2 For Triton x-ioo, each solution was 10±2p
Let it be pm.

Note that if the adjusted standard solution does not meet the accuracy shown in Table 2, the concentration is appropriately corrected, but the details of this concentration correction method will be omitted.

〔Effect of the invention〕

As is clear from the above examples, in the present invention,
When adjusting Na s K s Ca to a predetermined concentration, NaCl, which has low hygroscopicity, and 1° KCl are used as reagents.
Since CaC0B is used, stable and accurate weighing can be performed. Therefore, a standard solution having a highly accurate concentration can be stably prepared. In particular, Ca COB is difficult to dissolve using normal methods, but it is not hygroscopic, so it can be used as HC1!
Water-soluble calcium chloride CCaC12)
Since the standard solution is dissolved as a standard solution, a more accurate and stable standard solution can be obtained.

Measuring the four components of Na+, K+, Ca+, and pH concentration in blood requires an accurately calibrated measurement electrode, and the standard solution according to the present invention is optimal as a standard solution for simultaneous calibration of the above-mentioned measurement electrodes. It can be said that there is.

Voluntary procedure amendment 1, indication of the case 1982 Patent Rei No. 143168 2 Title of the invention Tansuum ion, potassium ion, cal/lamb ion and 33, El, t7 ・0 measurement voltage'"i0
Relationship with the same school 11 standard solution 09 miscellaneous case Patent applicant 4, agent 5 Date of amendment order 6, number of inventions increased by amendment 7, column for detailed explanation of the invention in the specification subject to amendment ( 1) Correct "measuring electrode" in line 3 of page 2 of the specification to "measuring electrode for measuring".

(2) “Calcium-
Public bulletin). " will be corrected as follows.

"As shown in Japanese Patent Publication No. 56-35052, there was only a standard solution for simultaneous calibration of calcium-sensitive electrodes and electrodes." (3) Page 3, lines 6 to 9 of the same. 's "Tris~de"
Correct as follows.

"Tris(hydroxymethyl)aminomethane (chemical formula is (HOCH2)3ONH2, hereinafter 'l'ris
), hydrochloric acid (hereinafter referred to as HCt), and Triton X-100 (trade name, chemical formula is HO(CH2CH20)nC6H4C9H19) which acts as a surfactant.'' Correct "L standard solution~tomo" on line 14 as follows.

"Calibration is usually performed by two-point calibration (high-concentration standard solution and low-concentration standard solution), but below, we will use the high-concentration standard solution with H
The standard solution and low concentration standard solution are called L standard solution.

For L standard solution (cleaning solution) and H standard solution respectively.

Claims (1)

[Claims] A predetermined amount of hydrochloric acid is added to a mixture of predetermined amounts of sodium chloride, potassium chloride, and calcium carbonate, resulting in CaCO_3+2HCl→CaCl_2+H_2O+C
A mixed solution in which the reaction O_2↑ has been completed is prepared, and a predetermined amount of tris(hydroxymethyl)aminomethane diluted with pure water is added to the mixed solution while stirring, and then pure water is added to make the entire mixture. A method for producing a standard solution for simultaneous calibration of sodium ions, potassium ions, calcium ions, and pH measuring electrodes, characterized in that the standard solution is made to have a predetermined amount.