JPS61197504A - Iodine mixture and disinfectant - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention provides iodine, water-soluble iodide. I? Contains lipinyl alcohol and boric acid, water-soluble, anti-disinfectant.

The present invention also relates to a method for dissipating the available iodine contained in an iodine solution at a constant rate. Immediately after its discovery, iodine was used medicinally, either by itself or in combination with various compounds, but 19
Around 1939, it was used to treat wounded people from war. Iodine is used in various forms as an excellent disinfectant for skin and internal and external wounds, and is also used as a disinfectant for surgical instruments and the like. Iodine has also been used to disinfect drinking water, swimming pools, etc., or to disinfect equipment and other objects that come into contact with microorganisms that are perishable and susceptible to disinfectant attack. Iodine is a virus,
It is an excellent therapeutic agent against a very wide range of microorganisms such as bacteria, spores, yeasts, molds, protozoa, fungi, worms, nematodes, etc. Moreover, iodine is relatively toxic to tissues. This is because iodine dissociates into iodine ions, and iodine ions are not toxic.

However, iodine has serious drawbacks: it is highly irritating and photosensitizing, and it destroys bacterial proteins and, to some extent, animal proteins as well. Iodine also has the disadvantage of having an unpleasant odor and being easily stained.

It has been discovered that certain compounds act as carriers or emollients for iodine. The term iodine mixture is intended to mean a product in which the surfactant in the component acts as a carrier and solubilizer for the iodine. Known carriers include polymeric substances such as starch and various synthetic polymers; synthetic polymers include vinyl lactam polymers and active hydrogen compounds with surface-active properties, commonly referred to as alkylene oxide condensates. Examples include polymeric oxyalkylene derivatives. Specific examples of suitable polymeric substances include polyvinylpyrrolidone, polyvinyloxideridone, polyvinylimidazole, polyvinylmorpholine,
Examples thereof include polyvinylcaprolactam, polyvinyl alcohol, and condensates of ethylene>xhyropylene oxide with alcohols, amides, and phenols.

Iodine mixtures generally enhance the bactericidal action of iodine and reduce its vapor pressure and odor. In addition, it suppresses the stain-prone nature of iodine and can be diluted with water.

Furthermore, the irritating effect of iodine is significantly suppressed in iodine mixtures. A very useful iodine mixture is obtained as a result of the interaction between iodine, potassium iodide and polyvinyl alcohol in aqueous solution. Such mixtures are described in Mokhnach's ``Iodine-polymeric compounds and their use in medicine and veterinary medicine''.
-High Polymers and Their
Use in Medicine and Veteri
nary Medicine”, (Botan, In
st-4m°Komar.

Va, Leningrad) Iodinol Me
d, Vet, Eksp, 1 inch.

l5sled, Akad, Nauk S, S-8-R,
Bot, In5t, 1967°5-20 (Russ,
)). This mixture is used to treat purulent surgical diseases, varicose ulcers, fever and chemical burns, and is also used to treat a wide range of diseases in animals. This mixture is an effective preservative and is effective against Duram positive and negative bacteria, viruses and molds.

Its chemotherapeutic index, ie the maximum tolerated dose that can be administered compared to the medical dosage of the mixture, is very large. However, it is known that the shelf life of such mixtures is somewhat limited.

Polyvinyl alcohol-boric acid-iodine complexes are also known to have bactericidal activity (ActaPolo
n Pharm, Vol, 20. A6. P45
5-7 (1963) and K11n Ocgna, 36
(1), p. 27-32 (1966)).

The present invention relates to an aqueous iodine solution having a wide range of bactericidal, fungicidal, virucidal and hygienic effects and a long shelf life. It can be used as a cleaning, sanitizing and disinfecting agent for living and inanimate objects. The iodine solution according to the invention also exhibits a unique temperature change phenomenon. The invention also relates to a method for dissipating the available iodine contained in an iodine solution at a constant rate. In summary, the present invention relates to a method of containing the following materials:

1k) Iodine about 0.00005 to 10% by weight b) Water-soluble iodide about 0.0001 to 20% by weight C) Polyvinyl alcohol about 0.001 to 25i
4i-tid) Boric acid about 0.001 to 10% by weight The remainder of the above composition may be water alone, or may contain various other components that impart special properties. For example, the stability of the solution is increased by adjusting the pH to a range of about 3 to 8, so that acids that are less susceptible to oxidation can be added for this purpose. Additionally, various alkali metal and alkaline earth metal salts may be added to the ingredients to adjust the permeability (tonicity) of the solution and make it compatible with living tissue. Similarly, common neutral cationic, anionic and nonionic detergents can be added to improve the wettability of the solution.

The present invention also includes a method for dissipating the available iodine contained in the iodine solution at a constant rate. This dissipation method is used not only with the method of the present invention but also with other methods, and is a method in which the following substances are included.

a) Sorbic acid or a soluble ring chain thereof 0.01 to 5% by weight b) Ethylenediaminetetraacetic acid or a soluble salt thereof approximately 0.01 to 5% by weight to an iodine solution according to the method of the invention and a similar iodine mixture without boric acid Its unique property is that its color clearly changes depending on the temperature. That is, such an iodine solution exhibits a dark purple color at room temperature, but in a low concentration solution, it becomes colorless at high temperatures, and changes to dark purple again when the temperature drops. Taking advantage of this property, the method of the present invention can also be used with a temperature visualization device.

To explain the present invention in more detail, the method of the present invention includes iodine, water-soluble iodide, polyvinyl alcohol, and boric acid, but does not include only these components, and in this solution, iodine is mixed with polyvinyl alcohol and boric acid. It forms a complex with boric acid and ionizes various compounds, such as water-soluble salts, to at least some extent.

In the method of the present invention, certain ingredients form complexes with the iodine and serve to moderate and stabilize the iodine, while retaining the fast-acting antibacterial, antifungal, and antiviral effects of iodine. That is, the method of the present invention eliminates various drawbacks of general alcohol or alcoholic aqueous solutions containing molecular or crystalline iodine, and maintains a broad antibacterial spectrum effect.

Compared to conventional iodine solutions, solutions according to the method of the invention have reduced odor and staining properties and have a reduced surface tension (to about 40-72 dyneAm2), resulting in good wetting and cleaning action. It is something that you have. Moreover, the iodine-mitigating effect achieved by the method of the invention increases its compatibility with living tissues, which is particularly important when it is used for ophthalmic or other mucous membrane applications. Furthermore, the method of the present invention allows addition of common detergents and physiological salts as appropriate, without impairing the bactericidal action of effective iodine.

In the method of the present invention, a solution containing about 0.00005 to 10 parts by weight of iodine, preferably about 0.002 to 5.0 parts by weight, is first prepared in the form of a concentrated liquid, and then, depending on the intended use, a solution containing iodine is prepared. It is diluted to a desired concentration (generally 5 to 10
Purified water or special dissipating fluids as described herein are used to dilute the concentrate (0 ppm effective iodine concentration).

The iodine mixture according to the method of the present invention destroys all bacterial growth tissue within 15 minutes at an effective iodine concentration of 1:200,000, and even in such a dilute solution, it has a color indicating antimicrobial activity. When it becomes colorless, its bactericidal activity disappears.

The water-soluble, preservative iodine solution produced by the method of the invention is approximately 0.
0001-20 weight, preferably about 0,004-1
Contains 0.0 weight of water-soluble iodide. Potassium iodide is preferred, but the well-known soluble salts of iodine such as sodium iodide or hydriodic acid may be used as well. This iodide helps to initially dissolve the iodine and ultimately simplifies the preparation of the solution.

Water-soluble iodide in solution forms an anion that reacts with iodine according to the well-known equation:

I + I M I3- The generated I3- ions are soluble in water and generate free iodine when used. Boric acid and polyvinyl alcohol are
It is unknown whether the complex is formed with either I3- or I3-.

A wide variety of polyvinyl alcohols are used in the mixtures of the present invention in proportions of about 0.001 to 25 parts by weight, preferably about 0.1 to 20.0 parts by weight. Polyvinyl alcohol of various qualities is commercially available from various sources, such as Elpanol (America E, 1.DuPont de Nemo).
Urs and Co., Inc.) and pinol (American Colton Chemical Co.) are used. Polyvinyl alcohol is a water-soluble synthetic resin obtained by adjusting the degree of polymerization of vinyl acetate and partially or completely hydrolyzing polyvinyl acetate. By varying the degree of polymerization and the degree of hydrolysis of the acetate groups and polymer chains, a wide variety of resins can be obtained for use in the present invention. Since the viscosity of the solution increases as the degree of polymerization increases, the most appropriate grade of polyvinyl alcohol can be selected from a wide variety of resins depending on the purpose of use. This also applies to the degree of hydrolysis of polyvinyl acetate. For example, Elpanol has a viscosity of 1.8-135 cp at 20°C and a degree of hydrolysis of 85-135 cp.
Grades that produce 4% solutions such as 100% are available. When polyvinyl alcohol is dissolved in water, a solution with a pH of 5 to 8 is obtained, and its stability is greatest at acidic pH. Depending on the concentration and nature of the polyvinyl alcohol used, the viscosity of the solutions of the invention at 20 DEG C. varies from 1.1 to 10.000 cp.

In the formula, X and y represent numbers corresponding to free hydroxyl groups and acetylated groups in the resin, respectively.

Polymers that are fully hydrolyzed and have 25% acetate groups are suitable for the mixtures of the invention.

A particularly suitable polyvinyl alcohol is one commercially available under the trade name Elpanol 5105J.

The properties of this polyvinyl alcohol are 88-90% hydrolyzed and the volatile components are less than 5%. The viscosity of this 4% aqueous solution is 5 cp at 20°C.

The boric acid used in the process of the invention stabilizes this mixture and increases its shelf life, and has a range of from about 0.0001 to
More than 10% by weight of boric acid is used, preferably about 0.05 to 5.0% by weight.

In order to further increase the stability, the hydrogen ion concentration i of the solution is
It is desirable to adjust the pH to a range of about 3-8, preferably 385-7. To adjust the pH, acids that are not easily oxidized, such as phosphoric acid, hydrochloric acid, acetic acid, sulfuric acid, and nitric acid, are suitable.

Depending on the purpose of use, it may be desirable to adjust the permeability of the solution. In order to make the water-soluble, antiseptic iodine solution according to the method of the present invention compatible with living tissues, it is necessary to
．． Zero permeability, which is desirable to have a tonicity equal to a 0% aqueous sodium chloride solution, can be adjusted by adding an appropriate amount of an alkali metal or alkaline earth metal salt. Examples of suitable salts include sodium chloride, potassium chloride, calcium chloride, magnesium chloride, and their corresponding phosphates, nitrates, and sulfates.

The wetting and cleaning action of the method of the invention is enhanced by the addition of neutral cationic, anionic and nonionic detergents.

Although a large number of suitable cleaning agents are well known in the art, the cleaning agent may be present in solution up to 25% by weight.The preferred method of the present invention involves first dissolving boric acid in water. ,
Next, polyvinyl alcohol is dissolved in this boric acid solution while heating and stirring. The amount of water used to dissolve the boric acid and polyvinyl alcohol is the majority (70 to 90% by weight) of the water present in the desired water-soluble, antiseptic iodine solution. After cooling the polyvinyl alcohol-boric acid solution, iodine and water-soluble iodide are added thereto as an aqueous solution. The amount of water used in the iodine-aqueous iodide solution is a small fraction (5 to 15 parts by weight) of the water present in the desired iodine solution. Then the pH of the solution obtained
The target iodine concentrated solution can be manufactured by adjusting the amount to a desired value and adding an appropriate amount of purified water.

This method is particularly effective because it does not require heating to dissolve the iodine. That is, the method of melting iodine without applying heat means that there is virtually no loss of iodine to the atmosphere.

The availability and stability of iodine in iodine solutions is determined by direct titration with sodium thiosulfate standards.

This classical redox reaction is well known in the field of iodine chemistry.

The water-soluble, antiseptic iodine solutions produced by the method of the present invention range in composition from concentrated solutions to dilute solutions that dissociate free iodine, and are useful for cleaning, sanitizing, and disinfecting living and inanimate objects. This mixture is sold in ready-to-use form as a stable dilute iodine dissociation solution, or as a concentrate for dilution as needed before use. Also, the iodine in the mixture according to the invention can be dissipated at a suitable rate.

There is an increasing need for techniques to sterilize diagnostic and prosthetic instruments applied within body cavities and to the corneal surface. Many diagnostic instruments and prostheses that are inserted into body cavities or applied onto living tissue can be effectively sterilized by briefly contacting them with an iodine solution according to the method of the present invention, and after contact, a layer of The active iodine disappears before the tool is used.

Medical diagnostic and prosthetic instruments that can be effectively cleaned and sterilized by the method of the present invention include a fundus tensiometer for measuring fundus blood pressure, contact lenses, a set of instruments for colotomy surgery,
Catheters, denture prostheses, intrauterine, intracavitary and rectal instruments, surgical and dental instruments, surgical insertion tubes and prosthetics,
Examples include oral and rectal thermometers, goniometer lenses and other eyepieces, diagnostic and therapeutic devices, ophthalmic prostheses, tongue-entering tips, needles, cystoscopes and plastic drug carriers for topical or injectable use. be done.

The method of the invention cleans the surfaces of inanimate and medical products.
K is also widely used for sterilization. For example, a morgue,
Food processing equipment, garbage dump, washroom (hospital bath)
It is used in dairy farming equipment, drinking water production equipment, animal breeding rooms, surgical rooms, washrooms, and sterilization rooms in pharmaceutical factories.

The method of the invention is particularly effective in combating infectious pathogenic microorganisms from epidermal and mucous tissues. The aqueous, antiseptic iodine solution prepared by the method of the present invention exhibits broad antibacterial activity against pathogenic bacteria or microorganisms in infected epidermis or mucous membranes at appropriate concentrations. This iodine solution can be used as a spray, spray or solution.

Methods within the scope of the invention are also useful before surgical procedures to prevent post-operative infections. For example, cleaning the ophthalmic blind tube before surgery with a specific iodine solution according to the method of the invention can significantly reduce the degree of contamination or complications after surgery. Also, applying iodine solution to the human body before surgical incisions can protect the patient from the chance of contamination or infection, and it can also help clearly mark the surgical site.

The method of the invention is particularly suitable for sterilizing contact lenses prior to application to the cornea. Hydrophilic contact lenses currently under development present significant problems of microbial contamination, but these hydrophilic contact lenses can be made from hydroxyethyl methacrylate alone or the latter combined with various crosslinkers and plasticizers. It is obtained by copolymerizing with an agent and absorbs up to 80 inches of cold water. These lenses also absorb various low-molecular-weight organic substances contained in the atmosphere and form complexes, and these organic molecules serve as a food source for microorganisms. Sterilization of lenses thus becomes an important issue since they provide an environment suitable for the growth of microorganisms. Chemical sterilization of hydroxyethyl methacrylate lenses using conventional disinfectants has had serious problems. Hydroxyethyl methacrylate lenses form complexes with most of the common preservatives such as pendelkonium chloride, chlorhexidine gluconate, sodium thimerosal, chlorbutanol and phenylmercuric acetate. Lenses treated with these common preservatives cause chemical irritation of the cornea, making it practically impossible to use known disinfectants. Boiling hydroxyethyl methacrylate lenses is not a completely satisfactory method of sterilization; if the ocular mucus material is not completely removed before boiling and remains in the lens, boiling may denature it within the lens. This reduces the clarity of the lens, causing discomfort and, in some cases, rendering the lens useless. Disinfecting hydroxyethyl methacrylate lenses by boiling or in an autoclave has the disadvantage that the geometry and porosity of the lenses undergo gradual physical changes.

For many applications, limiting the degree of dissipation of available iodine in an iodine solution is not necessary to achieve sterilization, tissue compatibility, and ultimate stability, but the iodine solution is functional. In some cases, it may be desirable for all available iodine (I2) to disappear after the iodine solution has acted. Basically, compounds with suitable redox potentials to cause the change 2→2■- and are commonly known as antioxidants are used to destroy the available iodine. Such compounds include alcohols, aldehydes, alkenes, alkynes, aromatic hydrocarbons, amides, quinones, oxyacids, sugars, amino acids, subsulfites, sulfates, thiosulfates, and mercapto groups. and polymers of unsaturated compounds and the like are used. Solutions of the above compounds are known to destroy all available iodine at different rates. Although many compounds are used in various industries, safety and tissue compatibility limit the number of compounds that can be applied to large animals or animals.

Examples of compounds applicable to humans and animals include sodium sulfite, sodium thiosulfate, sodium bisulfite, cysteine, methionine, ascorbic acid, sorbic acid, and disodium ethylenediami/tetraacetic acid. When an iodine solution is treated with equimolar or more of inorganic sulfites and thiosulfates, methionine, cysteine, and ascorbic acid, the available iodine in the iodine solution is almost simultaneously activated. In some cases, it may be desirable for the neutralization reaction to occur substantially immediately in this manner. If it is not necessary to maintain the bactericidal effect of the treatment system, the iodine can be very easily neutralized by the compounds mentioned above.

However, it may be desirable to dissipate the iodine slowly and at a predetermined rate. It is also necessary for certain prosthetics, such as contact lenses after being stored in storage containers, to retain their sterilizing effect after processing. In such cases, the iodine dispersion liquid must be bactericidal) and have a repellent bactericidal action. Thus, an ideal iodine dissipative solution should act as an antimicrobial preservative, rather than dissipate iodine, and should have good tissue compatibility.

An aqueous solution containing about 0.01 to 5 parts by weight of sorbic acid or a soluble salt thereof and about 0.01 to 5 parts by weight of ethylenediaminetetraacetic acid or a soluble salt thereof is used to dissipate the available iodine contained in the iodine solution. That's what I understood. This solution dissipates iodine at a certain rate, has no tissue compatibility problems, and can be used as a bactericidal antiseptic solution. This preservative dissipating solution is suitable for dissipating available iodine in an iodine mixture such as an aqueous, preservative iodine solution according to the method of the present invention. Commercially available iodine mixtures that provide a certain dissipation effect when treated with the method of the present invention include polyvinylpyrrolidone-iodine, nonylphenolethoxylate-iodine, soluble starch-iodine, β-cyclodextrin-iodine, polyoxyethylene-polyoxypropylene. Examples include condensate-iodine, ethoxylated linear alcohol-iodine, and the like.

The mechanism by which the dissipating solution according to the method of the present invention dissipates available iodine is unknown. Sorbic acid or its water-soluble salts and ethylenediaminetetraacetic acid in the aqueous medium depend on the iodine concentration in the iodine solution, the amount added to the dissipating solution, the concentration of the two components in the dissipating solution, pH, and temperature conditions. Iodine can be destroyed at a predetermined rate of 1 to 300 minutes. The dissipating liquid is about 2~
It is effectively used in a wide pH range of 10, and the higher the pH value, the faster the rate of effective iodine dissipation. Also, as the temperature rises above room temperature, the dissipation rate also increases. It is appropriate to use 1 to 1000 parts by weight of the dissipating liquid based on the weight of available iodine in the iodine solution.

An aqueous solution suitable for maintaining the sterilization properties of prostheses such as contact lenses is a water-soluble iodine solution prepared by the method of the present invention with a weight of 1.0 to 10.0% and a water-soluble dissipation rate of about 99 to 90% by weight of the method of the present invention. It can be manufactured by mixing weight factors. Prostheses, such as contact lenses, can be processed by floating them in an aqueous dissipative solution and then adding an aqueous, preservative iodine solution. The available iodine in the iodine solution has the effect of sterilizing the prosthesis and is slowly dissipated by the dissipative solution. After sterilization by active iodine and dissipation of iodine, the solution retains the sterilization properties of the treated prosthesis.

Iodine solutions according to the method of the invention and similar iodine mixtures without boric acid change color with temperature. That is, the solution exhibits a deep purple color at room temperature, but when the iodine concentration is low, the solution is colorless at high temperatures.

The intensity of the color is related to the temperature of the mawashi in the range of about 15 to 100 tZ'. A temperature sensing device can be manufactured by placing this iodine solution in a transparent container such as a sealed glass container.

A suitable mixture for use in thermosensor devices is 0.0% iodine cotton.
001 to 0.01 by weight, water-soluble iodide about 0.002
~ o, oz weight, polyvinyl alcohol approx. 0.025
~0.25 weight and cotton 99.72~99.972
It consists of a large amount of weight. The solution may optionally contain about 0.005-0.05% by weight of boric acid.

A temperature sensing device containing such a solution is used to visually see the temperature of the solution or its surroundings.

That is, it can be used for home or hospital purposes, and can also be widely used in industry as advertising boards and the like.

In addition to being used as a temperature visualizing device for industrial and medical purposes, it can also be used in toys and new products.

It is not clear why the iodine solution produced by the method of the present invention exhibits a unique color change, but it may be due to the strength of the atomic interaction between iodine and polyvinyl alcohol (and boric acid) and the different nuclear energies (heat). It seems to be related to the mobility of these atoms depending on the level.

Next, the present invention will be explained in more detail with reference to Examples.

The following examples illustrate preferred embodiments of the invention and are not intended to limit the invention. Incidentally, in the examples, the words "sand" and "sacent" mean percent by weight.

Example 1 The composition of a typical concentrate is as follows.

Iodine
2.0 Sodium Choride 2.
0% polyvinyl alcohol 20.0% (Elpanol 5103) Boric acid 2.0% pH adjustment with sulfuric acid 4 Addition of purified water 100% The above formulated solution is obtained as follows. First, boric acid is dissolved in purified water (approximately 85 liters of water is used), polyvinyl alcohol is dissolved therein under heating and stirring, and then cooled. Add iodine and potassium iodide to the obtained polyvinyl alcohol-boric acid cold aqueous solution with purified water (approximately 5-10% of the total amount of water).
) and add the remaining water after adjusting the ptr.

Example 2 To demonstrate the stabilizing effect of boric acid, the following formulation solution was prepared.

Iodine
0.1 Potassium Choride 0.
2% polyvinyl alcohol 2.5 t (Elpanol 5105) Addition of purified water 100. Solutions having the same composition as the above solution except for containing 0.5% boric acid were prepared, and the pH of each was adjusted to 5.5.

The available iodine in each solution was determined by titration with a thiosulfate standard solution. That is, immediately after the solution is prepared, it is placed in a -oz. sealed polyethylene container at about 70°F (21,11
), each solution was titrated for one week and one month after storage. The results are shown in Table 1. From the experimental data shown in the table, the addition of boric acid caused an unexpected result. It is clear that a significant stabilizing effect was obtained.

Table 1〉 5% boric acid containing solution 0.115% 0.110
0.108 Tiboric acid-free solution 0.115 Ti
0090% 0.078% Example 3 The following solution can be prepared in a similar manner to Example 1.

Iodine
0.05 Potassium Cholide
0.1 polyvinyl alcohol 2-0 poly(Elpanol 5105) Boric acid o1 Sodium chloride 09 Adjustment of pH with typhosphoric acid solution 5.0 Addition of purified water 100.0 Eye washing with the above solution for 1 to 2 minutes It is essentially used for surgical sterilization.

Surprisingly, the eye cannot tolerate pure iodine solutions with the same level of iodine content in this solution, but is able to adapt in this solution form.

Example 4 In a similar manner to Example 1, a solution is obtained which is used for the cold chemical sterilization of dental or surgical devices, prostheses and inanimate surfaces as follows.

Iodine
0.02 Sodium Choride 0
．． 02 Polyvinyl alcohol 1.0% (Pinol PA-20) Boric acid 0.2% Potassium chloride 0.5 pH with acetic acid
4.0 Addition of Purified Water 100.0% Example 5 A solution that can be used by contact lens wearers for sterilizing all types of contact lenses including hydrophilic lenses was obtained in the same manner as in Example 1. It will be done.

Iodine
0.004% potassium iodide
0.008% Sodium chloride 0.50% Potassium chloride 0.20% Vinyl alcohol 0,25% (Elpanol 5105) Boric acid 0.5% Adjustment of pH with hydrochloric acid 4.5 Addition of purified water 100.0% This solution Samples were placed in clear glass prescription bottles and exposed to light at 20C. The amount of available free iodine was analyzed weekly using the sodium thiosulfate titration method. There was no significant loss in available free iodine even after 65 days. Throughout the test period, the pH value of the solution was 4.5 and remained essentially at its original value. Similar tests were carried out on test solutions adjusted to higher pH values (above 7,8) and a small amount of iodine was lost over the same time course.

Example 6 The following experiment demonstrates the antibacterial action of the solution obtained by the method of the invention.

A broth culture of the microorganisms shown in Table 2 for 24 hours was centrifuged at 3000 rpm for 20 minutes. Decant the supernatant and dissolve the residue in saline phosphate buffer (P, B,
S, ). This bacterial suspension admonition 0.2ml
．． was added to 3.8 rr+t of test solution and 3.8 ml of saline phosphate buffer (control culture). In this case the dilution of the reagents was 1:20.

The resulting suspension was left at room temperature and tested after 5, 30 and 60 minutes. The test method involved successively diluting various bacterial suspensions with Tryptic Say broth containing a neutral buffer and a surfactant (Tween 80), and 10-fold dilutions were used. The prepared suspension was tested after 5 and 60 minutes. No loss of titer was observed during the test period. The test results are summarized in Table 2.

Example 7 The following tests were conducted to demonstrate the safety and usefulness of the solution obtained by the method of the present invention.

1. Apply 2 drops of the solution from Example 5 to each eye of two rabbits for 30 minutes.
As a result of conducting an experiment for two days (8 a.m. to 5 p.m.) in which the drug was injected every minute to one hour, no abnormalities were observed in the eyes of the rabbits.

2. Typical hydrophilic cable contact lenses were inserted into the eyes of two rabbits and the lenses were left in for three days until any reactions that normally occur after lens insertion had subsided. One lens was removed from 6 rabbits and replaced with a lens that had been stored in the solution of Example 5 for 48 hours, but no abnormality was observed in the eye tested over two consecutive days.

3. Insert a contact lens preserved in the solution of Example 5 into one eye of the rabbit, and a dull lens preserved in a 0.005% chlorhexisone gluconate isotonic solution into the other eye. did.

The eye with the latter lens had a reaction to chlorhexinone, but the eye with the test solution-treated lens had no abnormalities.

4. No abnormalities were observed when two drops of the solution of Example 5 were injected into human eyes three times at 30 minute intervals.

Example 8 The following self-sterilizing solution (referred to as Solution A) was prepared in a similar manner to Example 1.

Iodine
0. 1 Potassium churide 0.2% polyvinyl alcohol
2.5% (Elpanol 5105) Boric acid 0.5% Addition of purified water 100.0% Solution B was prepared according to the following recipe.

Sorbic acid 0.1 Thiethylenenoaminetetraacetic acid nonodium salt 0.1
% Sodium chloride 0.75% Potassium chloride 0.20+% Adjustment of pF (with 5% sodium hydroxide solution) Addition of 7.4 purified water The sterilizing properties of 100.0% solution B were confirmed by heating.

U.S. Pat. ,284
into the transparent part of a contact lens cleaning and storage device as shown in the issue. Solution B (sterilization preservation dissipation liquid) 4 m
1 was added to each device, 3-6 drops of Solution A (Oleotoxicant) were added to each, and the test lenses were immersed in each solution to disinfect. Some lenses initially turned yellow because they had the effect of concentrating available iodine. In these tests, a low concentration (3-4 drops) of Solution A minimized iodine staining. In all experiments, iodine dissipated from solution between 1 and 6 hours. And no adverse effects were seen with any of the lenses tested at that time.

No matter how many times the test was repeated, the results were the same.

Typical contact lenses were immersed in a mixture of solutions A and B in the proportions mentioned above for different lengths of time (1 to 8 minutes) and then inserted into the anterior cornea, and no harmful effects were observed. A had no adverse effects even when directly injected into the eyes of rabbits.

After deliberately contaminating a typical contact lens with active growth media of Staphylococcus aurens, Streptococcus biornes, Escherichia coli, Shutomonas elpanor and Candida alpicans, a mixture of solutions A and B described above was added for different times. Soaked. When 3 drops of the solution were added to solution B (the effective iodine concentration of the resulting solution was about 0.003 weight), complete sterilization occurred in 30 minutes.

Typical hydrophilic soft lens lenses, common Napolymethyl methacrylate lenses and silicone lenses, as described above, have an effective initial concentration of iodine of 0.003-0.01% for 5-12 minutes after being worn on the human cornea. solutions A and B of
It was treated with a mixture of These lenses continued to be worn by volunteers without any adverse effects.

Claims (1)

Claims: 1. About 0.00005 to 10% by weight of iodine, about 0.0001 to 20% by weight of water-soluble iodide, about 0.001 to 25% by weight of polyvinyl alcohol, and about 0.001% by weight of boric acid.
A water-soluble, antiseptic iodine solution characterized in that it contains ~10% by weight. 2. In the method for producing a water-soluble, antiseptic iodine solution, (
a) From about 0.001 boric acid to the total weight of the iodine solution
10% by weight and polyvinyl alcohol from about 0.001%
(b) about 0.00005-10% by weight of iodine and about 0.0001-20% by weight of water-soluble iodide are dissolved in a small amount of water; , (c) A method for producing an iodine solution, which comprises mixing the above two types of solutions.