JPS61195669A - Health food made from fomes japonicus - Google Patents

Abstract

PURPOSE:To obtain a health food having remarkable effect for the prevention of complication of thrombotic syndrome in a patient of diabetes, by using an extract obtained by extracting hot-water extract of Fomes japonicus first with an oxygen-containing organic solvent, and then with a fat-dissolving solvent. CONSTITUTION:The hot-water extract of Fomes japonicus belonging to Polyporaceae family is immersed in an oxygen-containing organic solvent such as ethanol and left stand at 20-60 deg.C for 2-6hr to effect the secondary extraction. The resultant secondary extract is immersed in a fat-dissolving solvent such as a mixture of chloroform and ethanol and left stand at 20-40 deg.C for 5-24hr to effect the tertiary extraction. The obtained extract or its purified product is added to a food in an amount to enable the intake of about 500-10,000mg of the component daily for adult.

Description

DETAILED DESCRIPTION OF THE INVENTION (Industrial Application Field) The present invention relates to a health food made from Ganoderma lucidum, and more specifically, the purpose of the invention is to prepare a hot water extract of Ganoderma lucidum using an oxygen-containing organic solvent. To provide a health food made from Ganoderma lucidum, which is characterized in that it contains an essential component of an extract obtained by performing a second extraction and a third extraction of this secondary extract with a fat-soluble solvent.

(Background of the invention) In recent years, overeating such as high-sugar poor diet and high-fat diet has become common, and adults
Many people, both children and children, are obese, and obesity brings about negative effects such as a decline in athletic ability and a decline in the age at which people become susceptible to adult diseases.

In particular, obesity increases the content of sugar and neutral fat in the blood, leading to diabetes, arteriosclerosis, and hypertension.

These obese people tend to have diabetes and hyperlipidemia, and in people with these tendencies, the enzymatic activity of platelet cyclooxygenase in the blood increases, resulting in platelet arachidonic acid metabolite TXB. , it is said that the production amount of IIHT increases.

By the way, blood platelets contain two types of enzymes, cyclooxygenase and lipoxygenase.

This cyclooxygenase converts arachidonic acid into TXB,
It has the function of metabolizing old IT, and lipoxygenase has the function of metabolizing arachidonic acid to 12-11ETH.

TXB muI metabolized by the above cyclooxygenase
IIIT has a platelet aggregation effect, and it is known that increased production of these substances causes thrombotic syndromes such as arteriosclerosis, cerebral thrombosis, and coronary insufficiency.

Therefore, it is thought that obese people may develop thrombotic syndromes such as arteriosclerosis, cerebral thrombosis, and coronary insufficiency.

(Prior art and its drawbacks) Conventionally, to treat thrombotic syndrome, drugs such as heparin are administered, but this heparin is not a prophylactic drug, but is used for emergency cases, and moreover, it cannot be administered by injection. It is necessary to obtain a doctor's diagnosis and instructions for treatment, and it has not been easily used to prevent thrombotic syndrome.

Therefore, there is currently no preventive food that eliminates the harmful effects of obesity in people (adults and children) before they develop thrombotic syndrome, and there is no risk of the transition from obesity to thrombotic syndrome in obese people and in the industry. There was a strong demand for the creation of foods that preventively avoid this.

(Progress of the Invention) Therefore, the inventor conducted intensive research to eliminate the above-mentioned conventional drawbacks, and found that the hot water extract of Reishi mushrooms was subjected to secondary extraction with an oxygen-containing organic solvent; It has been found that the product obtained by tertiary extraction of the product with a lipophilic solvent specifically inhibits the metabolic function of cyclooxygenase and inhibits the production of HllT and TXB2.

As a result, we performed a second extraction of this hot water extract of Ganoderma with an oxygen-containing organic solvent, and a third extraction of this second extract with a fat-soluble solvent to prepare a food product containing the extract obtained as an essential ingredient. The inventors discovered that this product can be easily taken orally as food and is highly effective in preventing complications of thrombotic syndrome in people prone to diabetes, leading to this invention.

(Solution Means) That is, the present invention performs a second extraction of a hot water extract of Ganoderma lucidum with an oxygen-containing solvent, and a third extraction of this second extract with a fat-soluble solvent. The present invention relates to a health food made from Reishi mushroom as an ingredient.

(Structure of the Invention) The reishi mushroom used in this invention is a mushroom that belongs to the family Arunodactaceae and is generally called Mannentake, and its scientific name is Galoderma rugidamaru (GAPODE).
RMA LUCIDUMUM KAR3P), and there are varieties such as red grass, black grass, white grass, seedling grass, and purple grass, and in this invention, any of these known reishi mushrooms can be suitably used.

In this invention, a hot water extract from Reishi mushroom is obtained as follows.

That is, Reishi mushroom is shredded and the shredded material is immersed in hot water at 60-80°C, preferably 70°C for 1 to 10 hours, preferably 5 hours, to extract the hot water-soluble components in the shredded Reishi mushroom into the hot water. After this, it is filtered to obtain a filtrate containing a hot water extract, which is concentrated and further dried under reduced pressure to obtain a primary extract in a dry state. Depending on the case, it may be in the form of a concentrated extract.

Further, in the present invention, the secondary extraction of the primary extract with an oxygen-containing organic solvent is performed as follows. That is, an oxygen-containing organic solvent, preferably ethanol, and in some cases acetone is added to the primary extract, and the mixture is heated at 20 to 60°C, preferably 40°C, for 2 to 6 hours, preferably 4 hours. Further, in this case, if it is at room temperature (25° C.), it may be left for one day and night.

After extracting the ethanol-soluble components in the dry product into ethanol, filtration is performed, the filtrate is concentrated, and the ethanol extract is further concentrated under reduced pressure to obtain the ethanol extract as a secondary extract in a dry state or a concentrated extract state. Get it.

In addition, in the present invention, 3
The next extraction is performed as follows.

First, a fat-soluble solvent is added to the secondary extract and left at 20-40°C for 5-24 hours. In this case, the fat-soluble solvent is
Those having a ratio of 10:IV/Vχ such as chloroform/ethanol mixture, chloroform/ethanol mixture, acetic acid ethyl ester/ethanol mixture, acetone/hexane mixture are preferable.

After extracting the fat-soluble components in the ethanol extract into a fat-soluble solvent, filtration is performed, the filtrate is concentrated, and the filtrate is further concentrated under reduced pressure to obtain a tertiary extract, which is a fat-soluble solvent extract, to a dry state. Get it.

The above-mentioned tertiary extract may be purified by removing impurities by column chromatography or the like, and then dried.

In the present invention, health foods can be prepared from the tertiary extract or its purified product according to conventional methods, that is, prepared into confectionery, soft drinks, staple foods, powders, or granules, as necessary. Additives such as bulking agents, flavoring agents, sweeteners, excipients, etc. may also be added.

In general, the health food according to the present invention may be prepared 8 times so that an adult can consume 500 to 10,000 mg, preferably 5,000 mg, of the tertiary extract or its purified product per day.

(Effects of the Invention) As described in 2 below, the calorific extract of Ganoderma lucidum according to the present invention was secondly extracted with an oxygen-containing organic solvent, and this second extract was thirdly extracted with a fat-soluble solvent. Health foods made from Reishi mushrooms, which are characterized by having extracts as essential ingredients, are ingested as food, so they are not treated as pharmaceuticals, do not require a doctor's diagnosis and instructions for treatment, and can be consumed at home. It has the effect of being easily ingested.

The effects of the present invention will be made clearer by describing Examples and Experimental Examples of the health food according to the present invention.

(Example) A detailed explanation of this invention is as follows.

That is, Reishi mushroom is shredded, 100g of the shredded material is immersed in hot water 3β at 70°C to extract the hot water-soluble substances in the shredded Reishi mushroom material into the hot water, and then filtered and heated. A filtrate containing water extract components was obtained.

Thereafter, the filtrate was concentrated and dried under reduced pressure to obtain a hot water extract in a dry state.

Next, 20 g of the hot water extract was immersed in the ethanol mixture and left for 4 hours to allow secondary extraction of the ethanol-soluble components of the hot water extract in the ethanol, followed by filtration, and the filtrate was concentrated and dried under reduced pressure. The mixture was solidified to obtain an ethanol-soluble component as a secondary extract in a dry state.

Next, the above secondary extract was immersed in a chloroform/ethanol mixture (10:IV/VX) 3x, which is a fat-soluble solvent, to extract the fat-soluble components of the secondary extract into the chloroform/ethanol mixture, followed by filtration. A filtrate containing a fat-soluble solvent extract was obtained.

The filtrate is then concentrated and dried under reduced pressure to dryness,
3 g of a fat-soluble solvent extract, which is a tertiary extract, is obtained in a dry state.

This purified powder has a pale yellow crystal appearance and has a majesty of 12
The temperature was 3-125°C.

(Experiment example) Rat blood was converted into normal Wistar-King system (7)
(250-350 g) with EDT^ added as an anticoagulant (5.8 mM), the blood was incubated at 220 × g
T: Centrifugation for 10 minutes, and the supernatant (platelet-rich blood) was further centrifuged at 1500 x g for 10 minutes.
Saline buffer (25mM IIEPES, 135mM
Wash twice with NacllPl+7.4) and resuspend at 2-3 μl protein/mj.

To this washed platelet suspension, the tertiary extract was added at various concentrations shown in Table 1 and pre-incubated at 37°C for 5 minutes. Then [1”C]arachidonic acid (0,0
5 μCi) and incubate for 5 minutes. The reaction was stopped with formic acid and the arachidone vinegar metabolites were extracted with EtOAc. The extract was dried with N2 gas. Add a small amount of EtOA to this
silica gel thin layer chromatography (TLC).
) and quantified (developing solution: chloroform: methanol: acetic acid: water = 90:8:1:0.8 V/V).

Radioactive substances were detected by autoradiography, the spots were cut out, and the radioactivity was quantified using a liquid scintillation counter.

Below is the blank space 1 Table 1 1 Product (ratio χ with comparative example) 11
112-HETE ITXB, IIIHT
IH-1 "-gate 1 1 ** 1100.0 1100.0 +
100.0 11 * l 101132.0
1116.0 +112.0 1+3+1
II 1 Primary 1100112B, 0 1101.0 '170
．． 0 11 draws 11111 1 draws 15001164.5 166.4138.5
11 things 1111'1 1 110001214.5 1 33.2 +
24.7 1 * The unit of tertiary extract is the concentration of tertiary extract added to the washed platelet suspension (μg/m6) ** Comparative example of concentration O As is clear from the above results, the concentration of tertiary extract used in this invention is The tertiary extract of grass has an excellent effect on inhibiting the production of TXB and former IT.In other words, the health food according to the present invention containing such a tertiary extract as an essential ingredient is effective in inhibiting the production of TXB and former IT. It can be seen that it has an excellent effect on preventing thrombotic syndrome.

Claims (1)

[Claims] (1) The essential ingredient is an extract obtained by secondly extracting a hot water extract of Reishi mushroom with an oxygen-containing organic solvent and thirdly extracting this second extract with a fat-soluble solvent. A health food made from Reishi mushrooms.