JPS61195357A - Vacuum blood-collecting tube - Google Patents

Abstract

PURPOSE:To separate quickly the drawn whole blood sample to serum and blood-clot by a bottomed tubular vessel which is internally held under the reduced pressure and can be tightly stoppered and use such vessel as it is as a serum-preserving vessel by manufacturing the above-mentioned vessel of a polyacrylonitrile resin and sticking a specific cyclic org. compd. to the inside wall surface of the vessel. CONSTITUTION:The cyclic org. compd., such as the compd. having the o-quinone ring expressed by the formula (R<1>-R<4> are H, hydrocarbon, polar substituent, for example, COOH, OH, NH2, SH, etc. or the residual group of a polycyclic compd.), in which the two adjacent carbonyl groups exist three-dimensionally on the same plane is dispersed together with an org. compd. or org. polymer having an amine salt or quaternary nitrogen and thixotropic powder such as pulverous SiO2 powder into isopropyl alcohol to prepare a dispersion. Such dispersion is coated on the inside wall surface of the vessel made of the copolymer resin consisting essentially of acrylonitrile and is dried. The inside of the vessel is evacuated and the vessel is hermetically stoppered by a butyl rubber or chlorinated butyl rubber plug to constitute the vacuum blood letting tube. The whole blood is drawn into such tube. The blood clots in a short period when the tube is rested at a room temp. The such blood is quickly separated to the serum and the blood-clot by a centrifugal sepn. and the tube is made stably preservable as it is for a long period of in a refrigerator.

Description

DETAILED DESCRIPTION OF THE INVENTION (Industrial Application Field) The present invention relates to a blood test container, particularly a vacuum blood collection tube used for collecting a whole blood sample from a subject and separating II by centrifugation.

(Conventional technology) In recent years, along with the remarkable progress in testing technology, blood tests such as serum biochemical tests, epidemiological tests for death, and RTN cell tests have become widely used, and are becoming increasingly important for disease prevention and early diagnosis. ζIt has come to make a significant contribution. Serum tests are the main part of blood tests, and the serum required for tests is usually collected in a blood test container, coagulated, and then centrifuged to form blood clots with different specific gravities (fibrin and blood cells). separated from the mixed gel-like mass).

As conventional vacuum blood collection tubes, those made of glass and those made of polymethyl methacrylate, which is a synthetic resin, are used, but these generally have the following X point.

After blood is drawn into a vacuum blood collection tube, it takes a considerable amount of time for it to coagulate, making it impossible to perform a test quickly. This has become a problem. Even with glass vacuum blood collection tubes, which are said to have a short blood coagulation time, it takes 40 to 60 minutes for the blood to coagulate after blood is collected, and synthetic resin vacuum blood collection tubes require a long time for blood to coagulate. Banko,
Requires leaving for 4 hours or more.

Another drawback of conventional vacuum blood collection tubes is that if coagulated whole blood has different specific gravity depending on the method such as centrifugation.
When collecting pure serum for testing by phase separation into L blue and blood clots, the separability of serum is generally poor.

That is, gel-like fibrin or blood clots tend to stick firmly to the tube wall, which poses the problem of drastically reducing the amount of [f[l blue] that can be collected. Therefore, there were problems such as interference with serum biochemical tests. Even glass vacuum blood collection tubes, which are said to have relatively good separability, suffer from the above-mentioned problems when used at low temperatures of 15° C. or lower, especially during winter.

On the other hand, inorganic fine particles such as glass are attached to the tube wall to shorten the blood coagulation time, and separation agents containing silicone oil, silicon compound powder, etc. are dispensed into the blood collection tube. It has been attempted to improve the separability of serum. Such separating agents have chicken-tropic properties;
It is adjusted to have a specific gravity between that of serum and blood clot. Therefore, after coagulation of the collected blood, when the blood collection tube is centrifuged, the separation agent moves to the middle of the If[Lrf1 layer and the blood clot layer, allowing separation from the serum layer. If it takes time to subject the thus separated serum to various tests, the blood collection tube is usually stored as is at a temperature of 4°C. However, the shelf life is relatively short, about 48 hours for glass blood collection tubes and 72 hours at most for polymethyl meccrylate vacuum blood collection tubes.

(Problem to be solved by the invention) This invention solves the conventional point X, which states "zero invention", and its purpose is to coagulate a collected whole blood sample in a short period of time, and then use a centrifugation operation to coagulate the collected whole blood sample. It is an object of the present invention to provide a vacuum blood collection tube that can effectively separate serum and blood clots.

Still another object of the present invention is to provide a vacuum blood collection tube that does not deteriorate the quality of serum even if it is stored at low temperature for a long time after centrifugation, and is therefore effective as a If11 serum storage container. It's about doing.

(Means for Solving the Problems) The gist of the present invention is to have a bottomed tubular container whose inside can be evacuated and a sealable stopper that maintains the reduced pressure state of the container, and the material of the tubular container is It is a polyacrylonitrile-based resin, and has a cyclic organic resin represented by the general formula (I) on the inner wall surface of the container, in which the two adjacent carbonyl groups are sterically located on substantially the same plane. A vacuum blood collection tube characterized by containing a compound, and having a bottomed tubular container that can be evacuated from the inside and a sealable stopper that maintains the reduced pressure state of the container, wherein the material of the tubular container is a cyclic organic compound which is a polyacrylonitrile resin and is represented by the general formula (I) on the inner wall surface of the s3, and in which two adjacent carbonyl groups are on the same steric plane; The vacuum blood collection tube is characterized by comprising an amine salt and an organic compound or an organic polymer having 414-grade nitrogen, thereby achieving the above-mentioned purpose.

Although the bottomed tubular container body of the Kokumei vacuum blood collection tube is made of polyacrylonitrile, it may be made of polyacrylonitrile copolymerized with methacrylate or a rubber component to improve moldability.

Polyacrylonitrile resins have excellent gas barrier properties9, which can be improved by maintaining a predetermined degree of vacuum.

In addition, it has excellent impact resistance compared to real glass and pyrimethyl methacrylate glue, and there is no risk of breakage due to reduced pressure. Regarding disposal after use, incineration, which cannot be done with glass vacuum blood collection tubes, becomes possible.

As the sealable Wk for maintaining the reduced pressure state of the vacuum blood collection tube, it is preferable to use one obtained by molding butyl rubber or chlorinated glutyl rubber according to a conventional method.

In order to reduce the pressure inside the blood collection tube, the blood collection tube may be sealed with a rubber stopper as described above in a reduced pressure container.

In the present invention, the cyclic organic compound represented by the above general formula that is present on the inner wall surface of the vacuum blood collection tube is used for the purpose of promoting blood coagulation, and the compound has two adjacent carbonyl groups and a residue. The homocyclic ring formed by the group A or F
It may be any of the i-ring cyclic compounds, and such cyclic compounds may be monocyclic or polycyclic compounds, but in the present invention, at least two carbonyl A cyclic compound in which the carbon-containing ring is a 6-membered ring or a 5-membered ring is preferably used.

In particular, the 6-membered cyclic compound preferably used in the present invention is represented by the following formula: It is a compound having an O-quinone ring represented by the above formula, and the hydrocarbon group in the above formula is not particularly limited, but is preferably an alkyl group, and the above polar substituent is also limited to special number ζ. Examples of such groups include carboxyl groups, carboxylic acid ester groups, hydroxyl groups, amino groups, and mercapto groups. Therefore, as preferred specific examples of compounds having an O-quinone ring, for example, O-quinone, gallic acid alkyl ester I oxide represented by the following general formula 0H (III) (wherein R11 represents an alkyl group); , (IV) (v) Partially oxidized nilazic acid and complete oxide, respectively, and 1,4-di(3,4-dihydroxyphenyl)2,3-dimethylbutane partial oxidation, represented by the following formula %. Examples include oxides and complete oxides.

Further, 1,2,3-triketohydroindane represented by (1) can be mentioned as a preferred specific example of a homocyclic compound in which the ring containing two complementary carbonyl carbons is a 5-membered ring.

Furthermore, one of the heterocyclic compounds that can be preferably used in the present invention, the following one-shot formula: R7 and R11 are hydrogen, hydrocarbon group,
Indicates a polar substituent or a residue in a polycyclic compound. ), where the above hydrocarbon group and polar substituent are the same as above.

A preferred specific example of such a compound is isatin represented by the following formula.

(X) All of these group of compounds have two adjacent carbonyl groups in the molecule that are sterically identical or approximately the same plane, and the detailed mechanism of action is unknown, but Shows specific effects on blood coagulation factors. On the other hand, an example of a compound that has adjacent carbonyl groups in the molecule but is not suitable for the purpose of the present invention because they are not on the same plane is, for example, 1,2-diketocyclohexane represented by the following formula. .

In this case, the alicyclic linkage that connects two adjacent carbonyl groups has a significant 7-lexiperity, and therefore these carbonyl groups are in gauche positions with respect to each other due to steric repulsion, and are not on the same plane. . This compound does not exhibit any specific effect on blood coagulation factors. It is speculated that these facts are probably due to the fact that two adjacent carbonyl groups in special steric positions have a special relationship with a specific site of the protein blood coagulation factor due to their steric structure. .

In the present invention, an amine salt or an organic compound or organic polymer having quaternary nitrogen is present on the tube wall surface together with the wall layer compound.

As the amine salt, both inorganic acid salts and organic acid salts of primary, secondary, and tertiary amines can be used. Specific examples include hydrohalic acid such as hydrochloric acid, sulfuric acid, Examples include salts of inorganic acids such as sulfurous acid, and weirs of organic acids such as formic acid and acetic acid.

The organic residue of the amine salt is usually an alkyl group, but this alkyl group may also be an alkyl group containing a different element such as an imino group or an ether group, and the amine salt shall include an inner salt. .

Therefore, specific examples of preferable amine salts include hexadacil dimethylamine salt, which is represented by the following formula %, and tetocdecyldi(aminoethyl)glycine, which is represented by the following formula %. .

Further, the organic monomer having quaternary nitrogen is preferably tetraalkylammonium, but it may partially have an aryl group, and as mentioned above, the alkyl group may be an imino group or It may also be an alkyl group containing a different element such as an ether group. For example, a preferred specific example is dodecyltrimethylammonicum chloride represented by the following formula %.

The organic polymer having quaternary nitrogen preferably has the following general formula (where RF to lu represents a hydrogen or alkyl group, X represents a halogen atom or an acid radical, Y represents an alkylene group or an alkylene group -so, -f represents ) is a polycation having a repeating unit represented by

In particular, a polycation having a repeating unit represented by the following formula % is preferably used.

In the present invention, the above-mentioned cyclic organic compounds, amine salts, or quaternary nitrogen-containing monomers or polymers are present on the inner wall of the container (in order to make them present, each component may be dissolved in a suitable binder or solvent). The coating may be applied by spraying or dipping in a dispersed state.

The amount of the above-mentioned cyclic organic compound and amine salt or compound containing quaternary nitrogen to be used is related to the amount of blood that is later injected into the blood collection tube, and the amount of each to be applied to the inner wall of the blood collection tube. Both are blood lvI! If the amount is less than this, the effect of promoting blood coagulation will be poor. However, if it is present in too large a quantity, it may cause various problems in blood testing. , lX1G''' is preferably 1g or less.

According to the method of the invention, the amine salt or
When the monomer or electrochemical complex containing heparin comes into contact with blood containing heparin, it adsorbs and neutralizes heparin, precipitates together with it, and quickly eliminates the action of heparin.
In this way, the normal blood coagulation function is restored, and the above-mentioned cyclic compounds have a specific effect on the blood coagulation function, so that the blood in the blood test container can be absorbed within a short period of time. The serum is coagulated and, therefore, can be obtained with good separation by centrifugation.

To explain this point in more detail, in normal blood, the contact between the blood and the inner wall of the blood container immediately activates factor #1 among the coagulation factors, which becomes the starting point for the chain reaction. Blood coagulation progresses reactively, and finally thrombin generated by activation of prothrombin acts on fibrinodan, forming an insoluble fibrin network and completing coagulation.

On the other hand, it is said that 1 to 10 units of heparin exist per 1 Qcc in the blood of patients receiving heparin administration. It acts synergistically with antithrombin present in the blood and significantly inhibits the action of thrombin. It is said that heparin not only inhibits the action of thrombin, but also inhibits the action of factor 1 and other coagulation factors. Therefore, blood containing heparin is usually In this case, the conversion of fibrinodan to fibrin does not occur, and as a result,
Serum cannot be separated because the blood does not clot.

However, according to the method of the present invention, as described above, since heparin is adsorbed to an amine salt or a monomer or a framework polymer having quaternary nitrogen and removed from the blood, thrombin While other blood coagulation factors restore normal blood coagulation, the specific action of the cyclic organic compound may significantly promote blood coagulation.

According to the present invention, as described above, heparin is removed from the 1fIL fluid, blood coagulation factors are rapidly activated, and the time required for coagulation after blood is collected into a container is significantly shortened. Separation of blood serum and blood clot becomes extremely easy, and therefore, the problem of blood clot components being mixed in the separated and collected serum is also solved.

Moreover, according to the method of the present invention, the blood clot components are sufficiently contracted, and as a result, the yield of serum is significantly increased.

However, in many biochemical tests, only serum is used, and after centrifugation using a blood test container, [f[lW
! In order to extract I, it is done by sucking it up with a pipette, but the preparative operation is time-consuming, and it is not suitable for transportation as it is.

For this purpose, a barrier-forming agent is present in the container. The septum-forming agent has thixotropic properties and is a composition that forms a septum by being positioned between the serum and 1fIL#P by centrifugation, and forms a septum so that the serum can be separated by decantation. It is a substance that

This septum-forming agent consists of a chicken-tropic agent and a viscous liquid.

Silica, alumina,
glass, talc, kaolin, bentonite, titania,
Inorganic powders such as zirconium, asbestos, and carbon black, and organic powders such as styrene resins, acrylic resins, and vinyl chloride resins are preferably used.

Among these chicken-tropic agents, fine silica powder shows the best results. Fine silica powder is a fine powder containing silicic anhydride as a main component, which has been subjected to hydrophobization treatment by grafting reaction or coupling reaction depending on the required number, and whether the raw material is naturally produced in powder form or not. It doesn't matter if it's lumpy or not.

However, the average particle size of these chicken-tropic fl agents is 1
It is preferable that it is mμ - 100μ.

If it is smaller than 1 mμ, it is difficult to handle, and when mixed with the viscous liquid described below, it tends to aggregate and form secondary particles, making it difficult to uniformly disperse it. This is because the dispersion stability is poor and the uniform fluidity of the partition wall forming process as a whole is affected.

Furthermore, when the specific surface area is 10 to 500 yr//P, excellent chicken tropism can be obtained.

However, when the specific surface area is smaller than 10 m'/P, the inorganic fine powder becomes difficult to mix with the viscous liquid that is a component of the separating compound, and sedimentation tends to occur. Large particles tend to aggregate, making uniform dispersion in viscous liquids difficult.

The viscous liquid may have a strong interaction with the thixotropic agent, but the viscous liquid may have a strong interaction with the thixotropic agent, and the viscous liquid may have good compatibility. It is also possible to use both of them together.

It has a strong interaction with the thixotropic agent,
Even if a certain chicken-tropy imparting agent is mixed with a certain viscous liquid and dispersed uniformly, the mixture is centrifuged for 30 minutes at 400 rpm in a centrifuge with an arm length of 10 cm. This refers to the case where no bias is observed in the distribution of components.

The cause of such interaction is not yet clear, but it is presumed that the cause is cohesive force caused by the molecular structure of the materials.

Examples of viscous liquids that have a strong interaction with the thixotropic agent include acid-modified products of liquid polymer substances such as acrylic resin oligomers, polyester oligomers, liquid polyisoprene, liquid polybutene, and liquid polygetadiene, especially acid-modified products of malemono-modified substances, Examples include animal and vegetable oils such as soybean oil, linseed oil, safflower oil, and fish oil, acid-modified products of the animal and vegetable oils, liquid polymer substances such as liquid polybutene and liquid polybutadiene, and epoxy-modified products of the above-mentioned animal and vegetable oils. When the imparting agent is an organic powder, examples thereof include oligomers of the same type as the thixotropy imparting agent, such as styrene oligomers for polystyrene, and it is preferable that all of them have a viscosity of 200 cps or more.

When such a viscous liquid material is used, the chicken tropism imparting agent and the viscous liquid material will not separate.

Viscous liquids that do not have strong interactions with thixotropic agents can also be used in the presence of water-insoluble amine compounds in the present invention. Such viscous liquids include liquid polymer substances such as liquid paraffin, liquid polyisoprene, liquid polybutene, liquid polybutadiene, styrene oligomers,
and their chlorinated products, and when the thixotropy imparting agent is a styrene resin, liquid paraffin and its chlorinated products can be mentioned. It is also possible to use mixtures of viscous liquids that have a strong interaction with the thixotropic agent and viscous liquids that have good compatibility therewith and do not have a strong interaction with the thixotropic agent. Good compatibility in this case means that no phase separation occurs even if both viscous liquids are mixed, uniformly dispersed, and then left at room temperature for one week.

When using a mixture of a viscous liquid that has a strong interaction with the thixotropic agent and a viscous liquid that has good compatibility with the thixotropic agent and does not have a strong interaction with the thixotropic agent, It can achieve excellent viscosity stability. In this case, the mixing ratio of both viscous liquids is determined within a suitable range by taking into consideration the strength of the interaction with each agent, but in general, chickentropy has a strong interaction with the agent. 10 to 600 parts by weight of a viscous liquid that does not have a strong interaction with the thixotropic agent is used for 100 parts by weight of the viscous liquid that has the effect.

By the way, the stability of viscosity over time is further improved by using a water-insoluble amine compound.

As water-insoluble amine compounds, those having at least one alkyl group having 8 or more carbon atoms in the molecule are suitable, such as dodasylamine, tetradecylamine, hexadacylamine, occudecylamine, dodecyldimethylamine, tetradecyldimethyl Examples include amine, octadecyldimethylamine, polyoxyethylene octadecylamine, and trioctylamine.

Although the above-mentioned amine compound is used, it has the property of being easily adsorbed on the surface of the chicken-tropic drug, and the chicken-tropic property has an interaction with both the drug and the viscous liquid. By acting on the stability of viscosity over time.

In particular, amines having an alkyl group with a leg prime number of 8 or more are suitable not only because they are highly water-insoluble and do not dissolve in separated serum or plasma, but also because they are highly water-insoluble and do not dissolve in separated serum or plasma. This is because the long chain alkyl group of the amine compound adsorbed on the surface of the agent is presumed to have a stabilizing effect on the interaction between the chickentropic agents.

By using the amine compound, the stability of the viscosity of the partition wall forming agent over time is significantly improved, resulting in excellent centrifugal separability and stability of the partition walls.

The ratio of the thixotropic agent to the viscous liquid is 100 parts by weight of the viscous liquid and 2 parts by weight of the thixotropic agent.
~15 parts by weight, and when a water-insoluble amine compound is further used, it is preferably α02 to 5 parts by weight.

The specific gravity of the partition forming agent is general number ζ, LO3~L at room temperature.
O8, because it requires a specific gravity intermediate between that of Fi serum and blood clot to form a septum.

When such a septum-forming agent is used, the serum and blood clot are separated by centrifugation, and a septum is formed at the interface between the serum and the blood clot, and once formed, the septum is not stable. Since it does not crumble even if the container is tilted, the serum can be easily separated by decantation or the like.

To obtain the vacuum blood collection tube of the present invention, first, acrylonitrile resin is processed by injection molding, blow molding, compression molding, molding, transfer molding, vacuum molding, casting molding, etc.
Shape: A tubular container with a bottom is formed, a blood coagulation promoter such as the cyclic organic compound is made to exist on the inner wall surface of the formed container by the method described above, the pressure is reduced if necessary, and the container is sealed. All you have to do is apply a possible stopper.

Further, the partition-forming agent may be dispensed from the beginning of the container square number ζ, or it may be added when performing the centrifugation operation after the collected blood has coagulated.

(Effect) When blood is collected from the subject into the thus obtained vacuum blood collection tube with a decompressed interior and left at room temperature, approximately 2
Blood clots in 0-30 minutes. When this is centrifuged, it is separated into serum and blood clots.

The material for the container of the vacuum blood collection tube of the present invention is polyacrylonitrile resin, which has low hydrophilicity. No molecular adsorption layer is formed. Therefore, the septum-forming agent and the inner wall surface of the blood collection tube come into close contact, completely separating serum and blood clots. Therefore, it is considered that inorganic ions from the blood clot do not enter the serum layer via the water molecule adsorption layer on the inner wall. Therefore, serum separated by centrifugation can be stored at low temperature for a long time. When the vacuum blood collection tube of the present invention is used, it can be stored at 4°C for 340 hours or more.

This is approximately 7 times as high as when using a glass blood collection tube. On the other hand, interval! l! When using a conventional vacuum blood collection tube in which a partition forming agent is dispensed, an adsorption layer of water molecules is formed, which prevents the partition forming agent from firmly adhering to the inner wall of the blood collection tube, and therefore the straining effect is not sufficient. It is thought that inorganic ions and the like diffuse from the blood clot to [[[111111].

As a result, separated serum cannot be stored for a long time.

As described above, when the vacuum blood collection tube made of the specific material of the present invention is used, the whole surface sample collected can be coagulated in a short time, and serum and [f[LI] can be effectively separated by centrifugation. Of course, after separation, the blood collection tube can be used as it is as a serum storage container.

(Example) The invention will now be explained based on Example V.

Example 1 Elazic acid oxide represented by the above Ozeki and the above formula (XV
A fractional solution of the polycation represented by I) in isopropyl alcohol was prepared. Acrylonitrile resin (which is made by copolymerizing this fractional liquid with acrylonitrile as a main ingredient and a small amount of getadiene and acrylic ester)
Product name Parex 210. It was coated on the inner wall of a 10 d capacity container manufactured by Mitsui Toatsu Chemical Co., Ltd. and air-dried.

The amount of the above compound added to the inner wall surface was 5×1.
0"g. Then, the inside was evacuated and the vacuum blood collection tube was sealed with a butyl rubber stopper, and the vacuum blood collection tube was set to R11. The degree of vacuum inside was set so that the amount of blood collected was 6 d. The vacuum blood collection tube was troweled. After fresh human blood was collected, it was left at 20°C, and the time required until the entire surface stopped flowing was measured as the blood coagulation time, and blood coagulation properties were evaluated.

Immediately after blood coagulation, centrifugation was performed at a rotational speed of 3000 rpm for 5 minutes, and % IfIL blue separation was observed. As is clear from the results in the Example 1 column of Table 1, blood coagulation in the obtained vacuum blood collection tube was extremely rapid.
The state of separation of the TU liquid was in good condition.

Example 2 Specific gravity at 20°C is LO2 and viscosity is 10.000 cp
Epoxidized soybean oil 2 containing 70 parts by weight of chlorinated polybutene of s and having a specific gravity of LO and a viscosity of 1700 cps at 20°C.
1 part by weight, 9 parts by weight of hydrophobized finely powdered silica, and 2 parts by weight of trioctylamine α were kneaded using a Miki roll mixer, and the specific gravity at 20°C was LO6 +! A forming agent was obtained.

This distance! ! ! A forming agent was injected into the container with the inner number Iy obtained in the same manner as in Example 1, the degree of internal vacuum was set so that the amount of blood collected was 6 d, and the container was sealed with a butyl rubber stopper.

Using the true 22 medical blood vessels prepared in this way.

Blood coagulation and serum separation status were observed in the same manner as in Example 1. Next, to observe the performance as an RFN blue storage container, RTNA! After separating, store under refrigeration at 4℃,
Immediately after the start of preservation and on the 3rd, 7th, and 144th day of preservation [f
[1llIl was collected and LDH and K listed as biochemical test items were measured.

As is clear from the results in the Example 2 column of Table 1, blood coagulation in the obtained vacuum blood collection tube #i was rapid and the serum separation state was also good. Furthermore, as is clear from the results in the column of Example 2 in Table 2, the container showed stable values for LDH and was suitable as a serum storage container.

Comparative Example 1 Using a commercially available 6-glass vacuum blood collection tube, the blood coagulation property and 1f11? were tested in the same manner as in Example 1. The IF separation state was observed. Next, the performance as a serum storage container was observed in the same manner as in Example 2.

The results are listed in the Comparative Example 1 column of Tables 1 and 2.

Table 1 Table 2 (Effects of the Invention) According to the present invention, the collected whole blood sample can be coagulated in a short period of time, and serum and blood clot can be effectively separated by centrifugation. A vacuum blood collection tube that can be obtained is obtained.

Furthermore, the vacuum blood collection tube of the present invention is also effective as a storage container for serum after centrifugation, and the obtained sample can be stored at low temperatures for a long period of time.

that's all

Claims (1)

[Scope of Claims] 1. The tubular container has a bottomed tubular container whose inside can be evacuated and a stopper that can be tightly plugged to maintain a reduced pressure state of the container, and the material of the tubular container is polyacrylonitrile resin; On the inner wall of the container, there are general formulas (I) ▲mathematical formulas, chemical formulas, tables, etc.▼ (where A indicates the residue of a cyclic compound), and the above two adjacent carbonyl groups 1. A vacuum blood collection tube characterized in that the cyclic organic compounds are present on substantially the same three-dimensional plane. 2. The vacuum blood collection tube according to claim 1, wherein the stopper is made of butyl rubber or chlorinated butyl rubber. 2. The vacuum blood collection tube according to claim 1, wherein the stopper is made of butyl rubber or chlorinated butyl rubber. 3. The vacuum blood collection tube according to claim 1, wherein a septum-forming agent is provided in the container. 4. The vacuum blood collection tube according to claim 3, wherein the septum-forming agent contains a thixotropic agent and a viscous liquid. 5. The vacuum blood collection tube according to claim 3, wherein the septum-forming agent contains a thixotropic agent, a viscous liquid, and a water-insoluble amine. 6. The tubular container has a bottomed tubular container that can be evacuated from the inside and a sealable stopper that maintains the reduced pressure state of the container, the material of the tubular container is polyacrylonitrile resin, and the inner wall of the container has a general Formula (I) ▲There are mathematical formulas, chemical formulas, tables, etc.▼ (However, A represents the residue of a cyclic compound), and the above two adjacent carbonyl groups are sterically substantially a cyclic organic compound on the same plane as
1. A vacuum blood collection tube comprising an amine salt or an organic compound or organic polymer having quaternary nitrogen. 7. The vacuum blood collection tube according to claim 6, wherein the stopper is made of butyl rubber or chlorinated butyl rubber. 8. The vacuum blood collection tube according to claim 6, wherein a septum-forming agent is provided in the container. 9. The vacuum blood collection tube according to claim 8, wherein the septum-forming agent contains a thixotropic agent and a viscous liquid. 10. The vacuum blood collection tube according to claim 8, wherein the septum-forming agent contains a thixotropic agent, a viscous liquid, and a water-insoluble amine.