JPS61155331A - Agent for removing mutagenic compound toxicity contained in food and drink - Google Patents

Abstract

PURPOSE:The titled removing agent comprising food fibers obtained from beet containing both water-soluble food fibers and insoluble fibers as an active ingredient. CONSTITUTION:An agent for removing mutagenic compound toxicity contained in food and drink in the digestive organs, containing food fibers as an active ingredient obtained from beet pulp (residue after extraction of soluble components such as saccharide, etc. from beet: e.g., table I), containing conjugated fibers of water-soluble food fibers such as pectin, hemicellulose, cellulose, etc. and insoluble fibers. Compressed pulp such as one shown by the table I is optionally ground and used in a powdery state. The agent is orally taken, mutagenic compounds existing in the digestive organs are adsorbed on it, mutagenic compound toxicity is eliminated and excreted out of the body. The amount of intake is sufficiently 3-15g/person/day as the active ingredient (5wt% water content) and the agent is mixed or processed with various foods.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to an agent that uses dietary fiber contained in sugar beet pulp to remove mutagen toxicity r4@ contained in foods and drinks.

More specifically, the present invention provides 1j! of dietary fiber contained in sugar beet pulp. The present invention relates to an agent that removes the toxic properties of mutagens contained in food and drink products within the gastrointestinal tract.

It has been announced that vegetables etc. have the effect of inhibiting the toxicity of mutagens, for example, "Environmental Mutagen Research" Vol. 5.1981. 29th Edition - 5th Edition.
On page 2, it was discovered that cabbage, broccoli, burdock, etc. have the ability to inactivate and suppress mutagens such as amino acids and protein products produced by heating, and that cabbage factor is a type of peroxidase. 'Chemistry and Biology'V
ol, 1842.

1981, No. 95-105, there are various descriptions of the nutritional effects of dietary *fiber, and the dietary fiber prepared from burdock, carrots, radish, bean sprouts, etc., has harmful substances such as Red No. 2 (amaranth). It is said to have a toxicity blocking effect.

Furthermore, the toxicity inhibition ability varies greatly depending on the type of dietary fiber, and burdock fiber is fractionated! The ability to inhibit amazine toxicity when prepared from cotton is similar to that of Holocellulomene neutral detergent-treated DF (NDF), 'burdock DF-treated DF (NDF)', acidic agent-treated DF (ADF) and cellulose, and pure cellulose prepared from cotton has no effect at all. It is considered invalid. In addition, the relationship between the physicochemical properties and effects of this dietary fiber shows a high correlation with water-retentivity (W, H, C) and submerged settling volume <SV>, and sometimes the submerged settling volume (SV)
) is said to have a greater ability to inhibit toxicity.

On the other hand, sugar beet pulp is the residue after extracting sugar from sugar beet during sugar beet production, and is either compressed and dried or discharged outside the factory without drying. It has a good taste and contains a lot of soil-based substances, so it is not edible and is used as feed. As proposed, it is possible to make an edible high-fiber food by crushing, washing with water, and sieving, or by crushing and sieving after heat treatment.

This sugar beet pulp contains water-soluble and insoluble fiber components such as cutin, hemicellulose, and cellulose, and is extremely good as a so-called dietary fiber.

The present inventors have extensively researched the dietary fiber contained in the above-mentioned sugar beets and J. lupus, and have found that the enzymes are completely inactivated, and the Sv (specific volume in water) is not large compared to other dietary fibers. This invention was realized based on the knowledge that the ability to remove mutagen toxins is extremely high despite the fact that it has a very high ability to remove mutagen toxins.

As mentioned above, sugar beet pulp is the residue after extracting 0T-soluble components such as sugar beet pulp, and an example of the components is the following.

In the invention, compressed pulp as shown in the table above is dried and, if necessary, pulverized for use in powder form.

As a result of tests on water retention and specific volume in water of powdered burdock fiber, butterbur fiber, wood cellulose, konjac mannan, sugar beet, and J-ruple dietary fiber with a particle size of 60 mesh, the results are shown in the table below. It was 9.

Test method Water retention: Use centrifugation method a) ? Add excess pure water to the sample, mix well, and then filter naturally to remove free water.

(b) Pack 100 d of sample from which free water has been removed into a sample cylinder (100,517 volumes) and use a centrifugal filtration device (horizontal type).
Dehydrate by centrifugation using T-T.

H] After centrifugal dehydration, determine the water content ratio (M amount percent) of the sample.

Specific volume in water: (a) Add approximately 501 Lt of water to the dry sample 19 and then degas it with a running water pump.

(b) Transfer this sample to a measuring cylinder, add a sufficient amount of water, and then, after reaching water equilibrium, measure the specific volume in water as the amount of swelling.In Table 2 above, the specific volume in water value is the highest for konjac mannan This is followed by butterbur fiber and burdock fiber, and sugar beet pulp dietary fiber does not have a particularly large value. That is, the sugar beet pulp dietary fiber used in the present invention has a specific volume in water smaller than that of konjac mannan, burdock root, butterbur, and other dietary fibers.

In addition, 1 of the fiber component composition of this sugar beet pulp dietary fiber.
An example is as follows.

As shown in Table 6 above, sugar beet pulp dietary fiber is composed of water-soluble and insoluble composite fibers such as hectin, hemicellulose, and cellulose.

The present invention relates to mutagen toxicity inhibiting efficacy using dietary fiber obtained from sugar beet pulp containing a composite fiber of water-soluble dietary fiber and insoluble dietary fiber as an active ingredient.

In the present invention, by orally ingesting sugar beet pulp dietary fiber, which is a water-soluble and insoluble composite fiber, it adsorbs mutagens present in the gastrointestinal tract, eliminates the mutagen's toxic ability, and leaves it as it is. It is excreted from the body.

Next, regarding the toxicity removal ability of these dietary fibers, Example 1
Sugar beet obtained from /J! A mutagen toxicity removal agent test was conducted using Lupu dietary fiber powder (PETAS: Trademark) and fractions of each composition obtained therefrom by the Ames method using each mutagen and Salmonella enterica. The results are shown in Table 4 below.

Note 1. λ is the product name (trademark) of sugar beet valve dietary fiber powder manufactured by Nippon Beet Sugar Co., Ltd. 2. This test was performed on mutagens Trp-P-1 and Trp-
0.5, 1.0, 0.1, 1.0.0 for P-2, Glu-P-1, Glu-P-2 and IQ, respectively.
Approximately 2% sugar beet pulp dietary fiber (dry matter) in an aqueous solution of 5 ppm O was mixed with Tasu and its fractions, left at room temperature, and after 1 hour, mutagenicity was determined using the Ames method (TA98+199 Mi).
x) was used to test the efficacy of suppressing mutations that cause cancer. The unit is the average number of colonies. However, this removes the number of colonies that naturally occur on the notebook.

3. The value in parentheses is the index 'f!' with the mutagen being 100. The 7-bit sugar beet pulp dietary fiber shown in Table 14 shows strong adsorption to carcinogenic mutagens.

It has a great ability to suppress mutations that cause cancer. That is, the mutation rate is zero for Trp-P-1, 25.41 for Glu-P-1, and 25.41 for Glu-P-1, and 25.41 for Glu-P-1.
It has an inhibitory effect of 17% for 1-P-2 and 5.9% for IQ. This suppressive effect is thought to be due to the fact that the dietary fiber in sugar beet pulp has the ability to adsorb carcinogens, and the dietary fiber in sugar beet pulp, which is porous and highly water-retaining, absorbs carcinogens and prevents them from being activated. . In addition, since this suction force is strong, it is thought that carcinogens are sucked up and crushed inside the body, and then excreted from the body in an inactivated state.

On the other hand, fractionated products such as hemicellulose, pectin, acidic detergent-treated fiber (cellulose + lignin), and neutral detergent-treated fibers [
Fiber (hemicellulose + cellulose + lignin) etc. are all inferior in removal effect compared to sugar beet pulp dietary fiber, and there is nothing comparable to sugar beet pulp itself.In the present invention, the water-soluble and insoluble fibers contained The effect of inhibiting mutagen toxicity is produced by the comprehensive action of the combined dietary fibers.

Sugar beet pulp dietary fiber, which is the active ingredient of the present invention, is sufficient to be consumed in an amount of 5 to 15,971 people/day based on 5 g of water. It is preferable to take it in the state.

Next, an example will be described. Example 1 In the sugar beet manufacturing process, 1 ton of cosette (moisture 89.2%) that has been leached from the leaching tower is immediately treated with hot water at 95°C, and the product temperature is maintained at 90°C or higher for 2 minutes. Dehydrated sugar beet cosette (moisture 72.4%) 676
I got g.

Next, dry with a drum rotary dryer to reduce the moisture content to 5.4
% dried sugar beet cosset (talent 3.2 arrogance) obtained 9 out of 108.

Next, the dried beet cosette was sieved through an 18-mesh sieve to remove all of the 6# that passed through the 18-mesh sieve, and the dried sugar beet cosette on the 18-mesh sieve was passed through a compact mill (hammer mill type, screen opening sieve). 150 Metu 7 Yu sieve powder (moisture 5.6%, ash 2.8%) 67g Jin Fu Gu.

The differential sugar beet pulp obtained here was used as the mutagen toxicity removing agent of the present invention.

5P Example 2゜ Male Wistar rats weighing 50 g were kept on basic diet, and the weight was reduced to about 100 g. After standardizing the weight, 10 rats each were fed 6
The experiment was conducted in groups.

One set contains Ca5ein 25%, Corn oil
59k, 8altmixture 4'%,
Vitamin m1xture 1 fk
, Cholin Chloride Q, 2%,
Vitaminεgranule O,05”4.5u
Close to make 100 Feed the basic feed, feed the basic feed to 2 groups, and then feed 1 or 2 groups.
- Dimethylhydrazine 3019 was administered via tube once a week at a rate of 9% body weight. Group 6 had Betus 10s as the basic feed.
1°2-dimethylhydrazine was administered in the same manner as in the second group.

The respective feeds were fed ad libitum.

Two sets of rats had a poor rate of pay increase, and four of them suddenly died of liver disease after 9 weeks; however, rats in sets 1 and 3 remained healthy even after 10 weeks, with no deaths.

Agent Masu Rishi 1) Parent male Procedural amendment C January 31, 1986

Claims (1)

[Claims] A mutagen poison removal agent whose active ingredient is dietary fiber obtained from sugar beet pulp containing a composite fiber of water-soluble dietary fiber and insoluble fiber.