JPS61118640A - Liquid injection apparatus - Google Patents

Abstract

PURPOSE:To variously inject a large number of liquids, by arranging six small apertures to one of opposed surfaces of a stator and a rotor so as to position on each of (m) pieces of concentric circles while arranging 3m pieces of bridge grooves and one bridge group communicating small apertures between the concentric circles to the other opposed surface CONSTITUTION:Small apertures (a)-(l) are formed on the concentric circles of the inner surface of a stator 21 at every 60 deg. and bridge grooves A-F for selectively communicating small apertures in the peripheral direction are formed to a rotor 24. The small apertures (a), (g) are selectively communicated by one bridge groove. The small aperture (a) is the inlet of an eluate and the small apertures (f), (l) are met to form the outlet of the eluate. The small apertures (b), (e) and (h), (k) are connected to both ends of a specimen loop and the small apertures (c), (i) are connected to a reagent container while the small apertures (d), (j) are connected to a suction pump. The rotor 24 is revolved by a handle 28 to communicate the small apertures (b), (c) at first by the bridge groove A to fill the specimen loop with a reagent and, next, when the apertures (a), (b) are communicated by the bridge groove A, eluates are met through both specimen loops to accurately and simultaneously perform the injection of two liquids.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention provides, for example, a flow injection analysis method (hereinafter simply abbreviated as F Tech A), in which two or more sample solutions are distributed and injected into an eluent. A multi-purpose liquid injection mode that allows the sample injection form and injection method to be changed simply by changing the flow path of the small opening of the device and the connection method of the sample measuring tube, and the above method for changing the flow path of one or more sample liquids. The present invention relates to a multi-purpose liquid injection/flow path change mode that can be changed simply by changing the method, and a semen fluid injection device that allows the flow path change to be changed simply by changing the method.

(Background of the Invention) FIA, which has recently attracted particular attention as a method for analyzing components in a sample liquid, involves injecting a sample liquid (hereinafter simply referred to as a sample) into a flow of eluent. Since the pressure applied to the system is high, a pressure-resistant liquid injection device is usually used to inject the sample.

Liquid chromatography (hereinafter simply referred to as HLO), which is attracting attention along with FIA, uses nine columns filled with a resin that has a high affinity for specific components in the sample, and the resin in the column is Based on the difference in affinity shown between the two, specific components are separated and analyzed using a detector.
This can be achieved by replacing the FIA reaction tube with a column, but depending on the analytical component, it is often necessary to select and use the most suitable column for the analysis from among multiple columns.

FIG. 1 schematically shows a typical liquid injection system in FIA, and a well-known hexagonal pulp 4 is used as the liquid injection device. FIG. 2 schematically shows a typical flow path changing system in HLO, and the two hexagonal pulps 11.13 described above are used as the flow path changing device.

To explain the outline of the liquid injection shown in FIG. 1, the solution 111 sent from the eluent tank 1 to the channel 6 by the pump 2 is normally delivered to one of the hexagonal pulps 4 which are in a communicating relationship as shown by the solid line in the figure. through two channels, channel 52 reaction tube, then detector 7
The liquid is passed through. On the other hand, the other two passages in the hexagonal pulp 4 are connected to a liquid metering fly (generally consisting of a tube enclosed in a buff rep, and simply referred to as a loop, henceforth abbreviated as such), that is, a loop 8. The sample in the sample container 9 is sucked by the pump 10 and the loop 8 is filled with the sample. Then, the passage in the hexagonal pulp 4 is switched as shown by the broken line in the figure, and the sample is injected into the flow of the eluent as a single plug flow, so that the sample and the reagent in the eluent react and are analyzed by the detector 7. It has become.

The hexagonal pulp 4 has a pair of stator (fixed body) and rotor (rotating body) that have airtight opposing surfaces, the stator has a total of 6 small openings at each 60° rotation position, and the rotor has It has six bridging grooves that communicate with adjacent small openings, and is well known as having a configuration that changes the communication relationship as shown in the figure as the rotor rotates 60 degrees. Since the injection mode of the sample into the eluent is simple and can be performed by rotating the rotor, it is now widely used.

In addition, to explain the outline of the flow path change shown in FIG. 2, the eluent sent from the eluent tank 1 is normally connected to the hexagonal pulps 11 and 13 in the communication relationship shown by the solid line in the diagram. The liquid is sent to the detector 7 through the channel. Therefore, the sample injected into the eluent will normally be separated by the column 14. Further, when the flow paths in the hexagonal pulp 11 and 13 are switched as shown by the broken lines in the figure, the sample will be separated by the column 12.

(Prior Art) The twelve-way pulp shown in FIG. 3 is known as one that enables these plural liquid injection forms by sliding rotation of one rotor.

This is a stator in which the 12 small openings of the hexagonal pulp are arranged in one stator, but in order to connect and use two pulps, the two small openings must be connected with piping. There was a dead volume at the time, there was a risk of contamination from the pipes, and two of the 12 small openings were actually used for communication pipes, and other pipes for other functions were connected. It had major drawbacks, such as being unable to produce pulp and being actually pulp pulp.

(Objective of the Invention) The first object of the present invention is to improve the injection style of the multi-build type, sample injection, and flow path change when multiple liquids such as a sample and a reagent are injected into an eluent as described above. Injection and flow path change method for multi-build type when simultaneous injection and flow path change for multi-build type when changing the flow path can be performed accurately by changing only the piping. The goal is to provide equipment.

(Summary of the Invention) According to the above-mentioned object, when injecting two or more types of liquids into other steady flows, the present invention enables the injection operation of a multi-build type system to be performed in a single manner by changing the piping configuration of the liquid system. The liquid injection device is characterized in that the liquid injection device can be operated only by rotating two rotors and a stator. A stator and a rotor that can be switched to the first and second positions by sliding rotation (n is an integer of 6 or more) have m pieces (m pieces) with different radii on one opposing surface.
m is an integer of 2 or more) concentric circles are formed, and one circle on the radial line of the angle obtained by dividing 360° on the concentric circle by n is 6
3xm small openings are arranged, and on one side of the pair of opposing surfaces there are 3xm bridging grooves that communicate the small openings, and on one side of the pair of opposing surfaces, there is one bridge groove that communicates the small openings between the concentric circles. A liquid injection device characterized by having a bridging groove.

(Embodiments of the Invention and Effects thereof) Hereinafter, one embodiment of the present invention will be described based on an embodiment shown in the drawings.

Figures 4 to 6 show multiple samples used for sample analysis in which at least the following sample injection forms (a) to (c) can be selected and performed only by rotating the rotor and stake by changing the piping form. A functional liquid injection device is shown.

(b) Injecting different samples simultaneously in parallel into the eluent flow (port) Injecting different samples simultaneously in series into the eluent flow (c) Continuously injecting samples , which shows the outline of the configuration of the liquid injection device of the present invention in a cross-sectional view, in which numeral 21 is a disc-shaped stator;
The tip of an annular flange of a rotor case 22 having a U-shaped cross section is engaged with the peripheral edge thereof, and the stator 21 and rotor case 22 are firmly connected by bolts 23.

In the hollow part surrounded by the stator and the rotor case 22, there is a rotor 24 which contacts the inner surface of the stator 21 in a predetermined suppressed air (liquid) state, and a drive disk for rotating the rotor 24. 25 and a spring 26 that applies a pressing force to the stator 21 of the rotor 24 through this drive disk 25 are housed, and a rotary drive shaft 27 is connected to the outside of the rotor case 22 from the back side of the front drive disk 25. has been extended. Reference numeral 28 denotes an operating handle that projects from the extending end of the rotary drive shaft 27 in the radial direction.

In the hollow inner surface of the stator 21, as shown in FIG.
A plurality of small openings a to t are formed as shown in FIG. There is.

In addition, the small openings azt are located at intervals of 60 degrees on the inner circumference from a to f, and at intervals of 60 degrees at 2 to t in the same direction as a to f on the outer circumference, and Al! :f are arranged in the illustrated relationship where they are communicated by a bridging groove.

As shown in FIGS. 6(a) and 6(b), the surface of the rotor 24 facing the stator has a small opening a-% on the stator side at a radially related position facing the small opening a%f.
A plurality of bridging grooves A to C that selectively communicate the circumferentially spaced portions of f and a plurality of bridging grooves D to F that selectively communicate the circumferentially spaced portions of the small openings 2 to L are formed. . These bridging grooves A to F are formed by providing recesses on the surface of the rotor in the present invention, but these are grooves that are opened only at both end portions of the rotor surface and are bored inside in the middle. But that's fine. Also, for convenience, the grooves are emphasized and drawn thick in the drawings, but in reality, fine line-like grooves are sufficient.

The stator 21 and rotor 24 configured as described above are brought into airtight contact with each other as shown in FIG. On the other hand, the position is changed by slidingly rotating the rotor 24.

FIG. 7 shows a flow diagram of FIA in which different types of liquids are simultaneously injected into the flow by the rotation of the rotor and stator using the sample injection device.

After the eluent is sent to the sample injection device 15 by the pump 1, it is sent to the detector 7 through the reaction Q-Pro.

FIG. 8 (A) shows a detailed piping diagram of the sample injection device 15 in the FIA of FIG. 7. The following description will be made based on the drawing.

The small opening a is an inlet for the eluent, and the small openings f and t are piped so as to join together and become an outlet for the eluent. Further, the small openings S, e and R are connected to both ends of the sample loops 8 and 81. In addition, small openings C and d are reagent liquid (second liquid)
It forms two points in the filling circuit, one of which C is the reagent container 52.
The other trench d is connected to a suction pump (not shown). Similarly, for small openings i and j, 1 is in a sample container (not shown),
The small opening j is connected to a suction pump (not shown). cut a
, f are occluded.

FIG. 8←) shows a state in which the stator and rotor are aligned in one position, which is the liquid filling mode, and this becomes the reference (first) position of the liquid injection device. In this state, liquid passages are formed in the small openings a and f, f and t by the bridging grooves C and F on the rotor side, and a steady flow is caused by the pump.

The small openings, c, d, and e of the sample system are still r2-+ A, -+ b-+ loop 13 → e- due to the bridging grooves A and B.
A liquid passage of +B-+0 is formed, and the loop 8 is filled with the sample using the sample container.

Similarly, in the reagent system liquid passage 1→D-+h→loop 8'→-
+B-+y' indicates that the loop 8' is filled with reagents.

From this state, the rotor 24 is slid and rotated by 60° relative to the stator 21 and switched to the second position, thereby changing the liquid passage as follows.

As the bridging grooves A, B, and O rotate, the communication relationships between the small openings a to f of the sample system are changed as follows.

That is, it is set to the other position which is the liquid filling mode shown in FIG. 8()), and A is the stator side booth opening a.

b, B are small openings c, d, O are small openings e, f0 Therefore, this liquid passage is between the inlet and outlet of the eluent, f −+
a -') A-)1)-+loop 8-) e →C
-) f, and the sample is injected into the flow of the eluent.

Still, change the alignment between the small opening 1-1 of the other reagent system and the bridging groove on the rotor side to position F, and the liquid passage is between the inlet and outlet of the eluent, from V to T. )-+h→loop 8'→→F→t, and the reagent is injected into the eluent flow.

Thus, in the injection mode at the second position, the eluent is divided into two stages at the inlet 2, one through the loop 8 and the other through the loop 8', and combined at the exit. Changes in the liquid passage are made in a complete cycle, and
Since the merging is caused by the flow of one eluent, extremely accurate simultaneous injection of the two liquids can be achieved.

That is, as shown in FIG. 9, it is possible to perform a "Pammerging Zone type" injection in which the sample and reagent are injected together in parallel.

(Effect of B) As a result, in FIA, which uses expensive reagents as eluents, it is possible to inject only the required amount of reagents when needed without using a liquid containing direct reagents as eluent. In addition, the pre-label HLO allows for the analysis of target components by reacting the target substance with a reagent and transporting the reaction product to a resin-filled column.
provides a simple play labeling device.

Figure 10 (a) shows an HLC system in which the sample injection device has a function of liquid injection and flow path change, in which the eluent and sample are transported to the column by changing the flow path during sample injection due to the rotation of the rotor and stator. A flow diagram is shown.

The eluent is sent to the liquid injection device 15 by the pump 1, and then to the detector 7 through the reaction tube 6.

FIG. 11(a) shows a detailed piping diagram of the liquid injection device 15 in the HLO of FIG.

The small opening f is the inlet of the eluent, and the small opening t is the outlet of the eluent. Further, the small openings S, e and R are connected to both ends of the sample loop 8 and the resin-filled column 12, respectively. Further, the small openings C and d connect two points in the sample liquid (second liquid) filling circuit, one of which is connected to a sample container (not shown), and the other d is connected to the shown suction pump.

Small openings 1 and j are open. j is connected to a suction pump (not shown). t is still occluded.

FIG. 11(b) shows a state in which the stator and rotor are aligned in the first position, which is the liquid filling mode, and this is the first position of the liquid injection device.

In this state, small openings a and f, f and t are formed by bridging grooves C and F on the rotor side.
A liquid passage of →t is formed, and the eluent is kept in a steady flow by the pump.

The small openings of the sample system, c, d, and e are C-) A-+ 1)-+ Loop 8-+ 6-+
A liquid path B −+ d is formed, and the sample container 9 fills the loop 8 with the sample.

Similarly, the column liquid passage 1→D-+h→column 12→ is opened by 1+E→j.

By slidingly rotating the rotor 24 by 60 degrees with respect to the stator 21 and switching it to the second position, the liquid passage is changed as follows.

As the bridging grooves A, B, and O rotate, the communication relationship between the small openings a to f of the sample system is changed as follows. That is, A is a small opening a on the stator side.

b, B is a small aperture c, d,' O is a small aperture e, f
Q Also, the bridging grooves E and F change the communication relationship between the small openings 2 to t of the reagent system as follows. That is, D is the small opening f on the stator side, and hXE is the small opening 1. jlF is +'% for the small opening, so this liquid passage is between the inlet and outlet of the eluent, f-+C-+e → loop 8-+ b → A-+
a −+ y −+ D −+ h−+ column 12−
+F→t, and the sample is injected into the flow of the eluent of normal liquid chromatography.

Accordingly, in the injection mode at the second position, sample injection and flow path change are performed simultaneously.

That is, the system normally changes from the no-load, no-column flow shown in FIG. 11 (b) to an HLO system only when necessary.

Still, replacing the column with a loop results in injecting the two loops of sample into the flow in series.

That is, as shown in FIG. 12(a), the sample and reagent can be injected in series.

If the sample and reagent filling circuits are replaced, it becomes possible to inject the sample first and then the reagent in series in the flow direction of the eluent, as shown in FIG. 12 (b).

(Effect of mouth) Since only the required amount of reagent is prepared when needed without using the sampled solution as an eluent, it is possible to save a large amount of reagent, and it is also possible to react the target substance of analysis with the reagent. In the PlayLabel HLO, in which the reaction product is transported to a resin-filled column and the target component is analyzed, a PlayLabel liquid injection device that enables the analysis method with simple operations is provided. In addition, in normal HLO, when the resin packed in the column is destroyed due to the properties of the liquid and the separation ability is reduced, injecting a reagent that changes the liquid properties of the sample before the sample is not necessary. It is very useful for protection.

FIG. 13(A) shows a flow diagram of liquid chromatography in which two columns are selected and used by the rotation of the rotor and stator using the sample injection device.

FIG. 14(a) shows a detailed piping diagram of the sample injection device in the HLC of FIG. 13(a).

The small opening f is an inlet for the eluent, and the small opening is an outlet for the eluent. Still small openings S, e and i, t are connected to both ends of the separation column 12.14. The small openings c, d and j are still open, and the small openings a and f are closed.

FIG. 13(b) shows a state in which the stator and rotor are aligned to the position where the separation column 12 is used, and this is the first position of the liquid injection device.

In this state, small openings e, f, a, b, W
, h is the bridging groove 0.0 on the rotor side. By A, D, f
-4) C-+6-+column 12→b-+A→a→V→
A liquid passage D→h is formed, and the separation column 12 is used as a separation column.

By slidingly rotating the rotor 24 by 60 degrees with respect to the stator 21 and switching it to the second position, the liquid passage is changed as follows.

As the bridging grooves A, B, and C rotate, the communication relationships between the small openings a to f of the sample system are changed as follows. That is,
A is a small opening on the stator side, c.

B is small opening d, e, C is small opening a, fo and bridge groove,
E and F also change the communication relationship between the small openings 1 to t as follows. That is, D is a small opening on the stator side, i and E are small openings j, and F is a small opening V.

t0 Therefore, the liquid passage is between the inlet and outlet of the eluent,
f −+ C−+ a −+ y −+ F −+ t
A liquid path called -+column 14-+i→D-+h is formed, and the column 14 is used as a separation column.

Also, by replacing the column with a loop, the sample can be injected at both locations.

As a result, in conventional sample injection devices, two positional relationships can be considered when sliding and rotating the stator and rotor.
Whereas only one of the positions was in sample injection mode, in the sample injection device, a sample is injected in both of the two positions in sample injection mode. In other words, the sample injection time is reduced to 1/2 of the conventional time.

(Effect of C) Regarding the effect of the liquid injection function, conventional HLC has made it possible to perform analysis in a short time due to significant advances in separation capacity, and F Engineering A analysis method is a flow-type analysis method that does not use separation. Because of this, it is possible to inject a large number of samples in an extremely short period of time, so in these fields it has been necessary to inject a large number of samples in an extremely short period of time. However, although conventional sample injection devices had limitations in sample injection due to the conditions described above, this injection device has made it possible to accurately inject a large number of samples into the flow of eluent in an extremely short period of time. Ta.

According to this embodiment described above, there are a wide variety of injection formats when injecting two types of liquid into the eluent flow.
It was difficult to achieve these simultaneously in one pulp. On the other hand, although the operation of this liquid injection device is extremely simple, just by changing the connection piping on the stator side, it has an excellent function of being able to select injection modes with many different functions. ,
Furthermore, the structure of the device itself is only a single pulp mechanism, and its usefulness is extremely large.

Furthermore, the grooves formed on the stator and rotor are actually fine linear grooves, and the rotor, which is usually made of polyimide, Teflon, etc., presses against the stator with a sufficiently large pressing force. Since it maintains sufficient air (liquid) tightness for contact, it is possible and easy to draw various grooves geometrically as long as it does not impede liquid flow. A multifunctional liquid injection device can also be designed with various groove formations and small opening arrangements.

Specifically, as shown in Fig. 15, the stator is the same as that shown in Fig. 5, but the shape of the bridging groove of the rotor is different, and the stator shown in Fig.
The figure shows that in the embodiments shown in Figures 4 to 6, the bridging groove that communicates the two circumferential small openings was placed on the stator side, but in Figure 16 (A) it was placed on the rotor side. Various aspects can be mentioned, such as those described in the following.

Furthermore, FIGS. 17(a) and 17(b) show a configuration diagram of a stator and a rotor of a liquid injection device which is one of the modified examples of the present invention in the case where n=7.

A plurality of small openings a-7 are formed in the hollow inner surface of the stake as shown in FIG. , and at the top of the circumference at 366/7 - about 51° from f
, f' are provided with bridging grooves. Similar to the trench, there are small openings 2 to t1 and bridging grooves t.

L' are also located on the outer circumference and arranged in the relationship shown.

As shown in FIG. 17(b), on the surface of the rotor facing the stake, at positions in the radial direction facing the small openings a to t, the circumferentially spaced portions of the small openings a to f on the stator side are selectively arranged. There is no problem even if a plurality of bridging grooves A to C are formed to communicate with each other, and a plurality of bridging grooves D to F are formed to selectively communicate the circumferentially spaced portions of the small openings 2 to L.

The basic structure of a liquid injection device with these functions is located on the circumference of a circle with m folds (m is an integer of 2 or more) at an angle of 360/n degrees (n is an integer of 6 or more). Among the small openings, a total of 6Xm small openings inside and outside are required, and one small opening on the inner periphery and one small opening on the outer periphery are always communicated by a bridging groove provided in the rotor or stator. characterized.

The above-described embodiment is based on the case where the small opening formed in the stator (or rotor) is m-2, but the present invention is also possible when m = 3.4 or more. FIGS. 18A and 18B are front views of the basic stator and rotor when m=3, and FIGS. 19A and 19B are m-4.

Other structures of the present invention include those that utilize sliding rotation of two stators and one rotor. )
That is, Fig. 20 shows the outline of the configuration of a liquid injection device that injects two types of liquid into a steady flow of a "5 sandwich type" using two stator surfaces and one rotor (two rotor surfaces). It is shown in a cross-sectional view.

In the figure, 21 is a disc-shaped first stator, 21' is a second stator, and these two stators 21, 21
' are firmly connected by bolts 23. In the hollow area surrounded by the stators 21, 21', air (liquid) is applied to the inner surfaces of the two stators 21, 21' under a predetermined pressure.
The rotor 24 is connected to the rotor 24 through the rotor 24 that is in contact with the rotor, the rotary drive shaft 27 that rotates this rotor, and the stator 21'.
A spring 26 that applies a pressing force to the two stators 21 and 21' is housed therein.

Reference numeral 28 denotes an operating handle that projects from the extending end of the rotary drive shaft 27 in the radial direction.

The stator 21 has a plurality of small openings a to f as shown in FIG. 21(a), and the stator 21'.

As shown in FIG. 21(b), a plurality of small openings 2 to t are formed in the stators 21 and 21', and these small openings are connected to various external pipes through through holes penetrating in the thickness direction of the stators 21 and 21'. ing.

There is a 22nd rotor on the surface of the rotor 24 facing the stator 21.
As shown in Figure (A), a plurality of bridging grooves A-0 selectively connect circumferentially separated portions of adjacent small openings on the stake side at radial positions facing the small openings a-f. However, as shown in FIG. 22(b), the surface of the rotor 24 facing the stator 21' is provided with the small opening f-.
In the radial direction facing t, a plurality of bridging grooves D to F are formed to selectively communicate circumferentially spaced portions of adjacent small openings on the stator side, and grooves formed on both surfaces of the rotor 24 are formed. One of them is communicated with @m and m' passing through the inside of the rotor.

FIG. 26 shows a piping diagram for injecting two liquids in series.

The small opening a is the inlet of the eluent, and the small opening V is the outlet. The small openings c, f and i, 1 are at both ends of the sample loop 8.8', and the small openings d and e, J and k form two points in the sample liquid filling circuit.

In FIG. 25, which is the liquid filling mode, a → 171-
A liquid path is formed, and each sample system is filled with a sample into the loop 8°8' by a liquid filling circuit.

In FIG. 23, when the rotor 24 is slid and rotated 60 degrees clockwise relative to the stator 21 and switched to the second position, the liquid passage is changed as follows.

a-+C-+f→Loop 8-+ O-+ A-+ m
↓ f(-F(-t(-loop 8'←1←D←m',
Two samples are injected in series into the eluent stream.

In the present device as well, as in the above device, there is no problem in using various configurations of m) 3, small openings in the stator, and various structures in the grooves in the rotor.

(Scope of application of the present invention) The liquid injection device of the present invention is not only applicable to sample analysis such as flow injection analysis, but also to mixing three or more liquids (or a combination of two liquids) for purposes other than analysis operations. It can also be suitably used as a storage device (which may also have functions such as warming). For example, it can be used to appropriately supply necessary reagents in a reaction system that performs a reaction using a fed reagent.

A specific example is the delivery of reagents to a reaction tank in the usual chemical industry or a fermentation tank in microbial fermentation.

(Effects of the Invention) As described above, the liquid injection device of the present invention is capable of injecting a plurality of liquids in various ways, and the function can be selected by an extremely simple operation of changing the piping. Moreover, these functions can be realized by rotating the rotor and stator extremely easily, the structure is simple, and injection can be performed accurately at the same time, making it extremely useful. There is something.

[Brief explanation of the drawing]

Figure 1 is a diagram showing an example of a liquid injection device using known hexagonal pulp, Figure 2 is a diagram showing an example of a companion flow path changing device using two known hexagonal pulps, and Figure 3 is a diagram showing an example of a known fluid injection device using two hexagonal pulps. A diagram showing a twelve-sided pulp, FIG. 4 is a schematic sectional view of a liquid injection device in an embodiment of the present invention, FIG. Figure 6 (A) is a front view of the rotor, ←) is a sectional view taken along the line ■-■, Figure 7 is a diagram showing an example of the flow using the liquid injection device of the present invention, and Figure 8 (I )
Figures 1 and 2 are diagrams showing an example of the piping of the liquid injection device of the present invention, (b),
(c) Figure shows the state of liquid passage formation by the stator and rotor, Figure 9 is an explanatory diagram when injecting the reagent and sample,
10(f) to (c) are diagrams showing an example of a flow using the liquid injection device of the present invention, FIG. 11(a) is a diagram showing an example of piping of the liquid injection device of the present invention, and (b) The figure shows the state of liquid passage formation by the stator and rotor, Figure 12 is an explanatory diagram for injecting reagents and samples, and Figures 13 (a) to (c) are flowcharts for using the liquid injection device of the present invention. Figure 14 (A) is a diagram showing an example of the piping of the liquid injection device of the present invention, Figure (B) is a diagram showing the state of liquid passage formation by the rotor and stator, Figures 15 and 16. is the front view of the rotor, the first
7.18.19 (A) is a front view of the stator, No. 17.1
8. Figure 19 (B) is a front view of the rotor, Figure 20 is a schematic sectional view of a liquid injection device in an embodiment of the present invention, and Figure 21 (
FIGS. 22A and 22B are front views of the stator, FIGS. 22A and 22B are front views of the rotor, and FIG. 23 is a diagram showing an example of piping in the embodiment of FIG. 20. 1; Eluent tank 2; Pump 6.5; Channel 4, 11, 13: Hexagonal pulp 6
; Reaction tube 7; Detector 8.8'; Loop 9; Sample container 10; Suction pump 12, 14; Column 21; Stator 22; Rotor case 23; Bolts
24; Rotor 25; Rotation drive disk 26: Spring 27; Rotation drive shaft 28; Operation handle 29; Tubes A, B, C...L; Bridge grooves a, b, c**ex: Small opening Fig. 5 ) Child Figure 6 □ Figure 8 (C) U) ! 1 piece q 1 piece 0 pieces N Hele Figure 19 (A) Figure 19 (B)

Claims (2)

[Claims] (1) A pair of airtight opposing surfaces are 360/n degrees (n
is an integer of 6 or more) In the stator and rotor that can be slid and rotated to switch to the first and second positions, m pieces with different radii on one opposing surface (m is an integer of 2 or more) concentric circles are formed, six small openings are arranged in one circle on a radial line of an angle obtained by dividing 360° on the concentric circle by n, and the small openings are communicated with the other opposing surface. 3
A liquid injection device characterized in that it has xm bridging grooves, and one bridging groove on either one of the opposing surfaces that communicates the small openings between the concentric circles. (2) Two pairs of airtight opposing surfaces are 360/n degrees (n
is an integer of 6 or more) In a stator and a rotor that can be slid and rotated to switch to the first and second positions, m pieces (m is 1 (an integer greater than or equal to m'(m' is an integer greater than or equal to 1) concentric circles with different radii are formed on the opposing surface of one of the remaining pairs, and 360° of each circle is formed by n. Six small openings are arranged on the circumference on the radial line of the angle divided by A liquid injection device characterized by having one through groove.