JPS61109716A - Agent for inhibiting growth of dental caries bacteria - Google Patents

Abstract

PURPOSE:An agent for inhibiting growth of dental caries bacteria, having high antibacterial action and pleasant aroma, containing maltol and/or ethylmaltol as an active ingredient, comprising components wherein safety to human body is observed. CONSTITUTION:Maltol and/or ethylmaltol shown by the formula I-II, a flavor substance wherein safety to human body was already observed, widely used as a flavor for chocolate products, or a flavor enhancer, is used. A tablet cake, chewing gum, candy, troche, or oral cavity composition such as dentifrice, mouthwash, etc. having a relatively long retention time in the oral cavity is blended with 0.005-10wt%, preferably 0.01-0.5wt% maltol and/or ethylmaltol, to give an agent for inhibiting growth of dental caries bacteria, capable of suppressing multiplication of bacteria represented by Streptococcus mutans and preventing formation of bacterial plaque.

Description

Detailed Description of the Invention (Industrial Application Field) The present invention is directed to the treatment of Streptococcus mutans, which is greatly involved in the formation of dental cavities.
The present invention relates to a caries growth inhibitor that has excellent antibacterial activity against oral bacteria such as C. ccus mutans.

(Prior Art) Chlorhexidine, antibiotics, and the like have been known to be used as drugs that inhibit the growth of oral bacteria, and many synthetic and natural products have been reported.

(Problems to be Solved by the Invention) However, these known oral bacteria growth inhibitors may have problems with their safety to the human body depending on the administration method and dosage, or their stability has not been confirmed. has low antibacterial properties or when administered.

There were drawbacks such as an unpleasant odor.

The purpose of the present invention is to ensure safety for the human body,
It is an object of the present invention to provide a caries growth inhibitor that has high antibacterial properties and a pleasant aroma.

(Means for Solving the Problems) According to the present invention, a fungal growth inhibitor characterized by containing maltol and/or ethyl maltol as an active ingredient is provided.

The present invention will be explained in more detail below.

Generally, the cause of cavities (caries) formation is the tooth structure (τae).
th), Substrate, and Streptococcus mutans (St), and these 3
Cavities (tooth decay) are said to occur when these two factors exist at the same time. The mechanism of cavities (dental caries) formation is 1. For example, when sugar is used as a substrate, this sugar becomes Streptococcus mutans (St, mutan).
Glucoxytransferase (enzyme) produced by s)
When absorbed, it changes into water-insoluble and sticky dextran (glucan), forming dental plaque on the surface of teeth. This dental plaque becomes anaerobic and fermentation proceeds by lactic acid bacteria and the like, producing lactic acid and lowering the pH. It is said that this lactic acid demineralizes tooth enamel and forms cavities (cavities).

Therefore, Streptococcus mutans (St, s
Suppressing the growth of oral bacteria, such as utans), and inhibiting the formation of dental plaque is an effective method for preventing the formation of cavities (cavities). Streptococcus mutans (St, mutans), which is greatly involved in the formation of negative (dental caries)
) As a result of intensive search and research for substances that meet the conditions for inhibiting the growth of the following, it was discovered that maltol and/or ethyl maltol are very effective, leading to the completion of the present invention.

Maltol and ethylmaltol used in the present invention are already known substances, and the different chemical structural formulas and chemical names are shown below.

The maltol used in the present invention is a commercially available product such as pyromatol (Gaikyo Kagaku ■, trade name) and peltol (Pfizer ■, trade name), and the ethyl maltol is a commercially available product such as viromatol E (Otsuka Chemical ■, trade name) and peltol. Plus (Pfizer, commercially available product) can be used.

Maltol and ethyl maltol used in the present invention are G
RAS list (Na2656.Na3487) and F
DAIJX (li121-1164.1121-11
64) Each of the above fragrance substances has already been confirmed to be safe.

Flavoring agents or flavor enhancers
(cer) such as chocolate products, breads,
Sugar confectionery, alcoholic beverages, meat products, dairy products, ice cream, canned foods, tobacco, toothpaste, lipstick, health products,
Widely used as animal feed. [Overview of fragrance chemistry (■
), Osamu Okuda, 904 pages, published by Hirokaku Shoten].

It is surprising that a flavoring substance that gives off a pleasant aroma in the oral cavity exhibits excellent effects as a growth inhibitor of caries bacteria.

The maltol and ethyl maltol of the present invention may be used in combination, or one may be used alone. In addition to being expected to be effective when incorporated into long tablets, chewing gum, candies, troches, etc., the caries growth inhibitor of the present invention has almost no antibacterial activity due to surfactants, so it can be used in oral cleaning agents such as toothpastes and mouthwashes. It can also be used in combination with The amount incorporated is not necessarily uniform depending on the form of the target product, etc., but it is preferably 0% to 10% by weight in the composition for oral cavities.
．． Generally, it is blended in an amount of 0.01 to 5% by weight.

Examples of formulations containing the caries growth inhibitor of the present invention are shown below.

Formulation Example-1 Chewing gum having the composition shown below was manufactured according to a conventional method.

1 1 1 1 1 Weight % Gum base
20 powdered sugar 35 maltose 10 fructose
10 water candy
15 Fragrance 1 Maltol 0.4 part 100% Prescription Example-2 A mouthwash having the composition shown below was manufactured according to a conventional method.

1 imaginary - 11 weight% ethanol
20 saccharin
0.5 Glycerin 50 Sucrose laurylate 2 Chlorhexidine 0.5 Flavor 1 Ethyl maltol 0.5 Water 100% Formulation Example 3 A troche having the composition shown below was produced according to a conventional method.

-1--" L-1 --JU1 Rainbow-- Gum Arabic 8 Maltose
30 fructose
20 Glucose 20 Flavor
0.2 Maltol 0.2100% (Example) Next, the antibacterial activity effect of maltol and ethyl maltol of the present invention will be explained in more detail with reference to Examples.

Example-1) 1% peptone, 0.5% yeast extract, 2% sucrose.

Prepare an agar medium with a pH of 7.0, and after sterilizing and cooling it, add 2
Streptococcus mutans (S
treptococcus mutang) RI M
0.5% of the culture solution of strain D-3125001 was added and immediately dispensed into 12-diameter sterile Petri dishes.
Next, a pepper disk with a diameter of 8 cm was impregnated with the sample compound, placed on the center of the agar medium of the above Petri dish, and heated at 37℃ for 7 days.
The cells were incubated inverted for 2 hours.

The antibacterial activity test (preliminary test) was evaluated as follows. A transparent agar medium becomes cloudy when bacteria multiply, but if the sample has antibacterial activity, the area around the pepper disk will remain transparent, so this clear zone (hereinafter referred to as the inhibition circle diameter) will be measured to determine the inhibition. A circle diameter of 401 mm or more was evaluated as having an effective proliferation inhibiting effect.

From the results in Effect Table 1, maltol showed an excellent bacterial growth effect, and ethyl maltol in particular showed a very remarkable inhibiting effect.

Example-2).

Heart infusion agar medium supplemented with 2% sucrose (p
Streptococcus mutans was used as in Example 1 except that H7.0) was used, and the same method was used. As a result, the results shown in Table 2 below were obtained.

(Margin below) Table 2 Streptococcus mutans From the results shown in Table 2, maltol showed an excellent growth inhibiting effect, and ethyl maltol in particular showed a very remarkable inhibiting effect.

Example-3) Streptococcus mutans (Streptococcus mutans)
coccus mutang) RI M D-31
The results were carried out using the same medium and the same method as in Example 1, except for using the 25002 (E49) strain.
The results shown are obtained.

Table 3 Effect on Streptococcus mutans From the results shown in Table 3, maltol and ethyl maltol showed extremely remarkable effects on inhibiting bacterial growth.

Example-4) 0.02% maltol (1/5
Streptococcus mutans (Streptococcus mutans), which had been pre-cultured
0.0.0.0.
5% and cultured at 37°C in a nitrogen desiccator for 24 hours, and the growth status of the bacteria was checked. As a result, there was no decrease in the pH of the culture medium, and no glucan formation was observed. We were able to prevent the growth of bacteria. In addition, for the purpose of practical use of this sample, sodium lauryl sulfate 0
．． When antibacterial activity was measured in the presence of 5%, no decrease in activity was observed compared to when no additive was added.

Example-5) The test sample was 0.02% ethyl maltol (11500G
The same medium (pH 7,
0), the bacteria and method showed that there was no decrease in the pH of the culture medium and no glucan formation was observed, making it possible to completely inhibit the growth of the bacteria.

Claims (1)

[Claims] A caries growth inhibitor characterized by containing maltol and/or ethyl maltol as an active ingredient.