JPS61104780A - Cultivator - Google Patents

Abstract

PURPOSE:In a cultivation tank, a specific stirring shaft is allowed ton rotate at a prescribed rotation with sufficient driving torque which is given through walls by magnets to keep the tank completely free from germs and effect animal cell cultivation in the tank. CONSTITUTION:A stirring shaft 2 which is provided with stirring blades 12, driving elements 3, 4 bearing magnets on both top and tail end is supported with two bearings, the one 10 which is supported with arm 11 fixed to the inner wall of the reactor and the other 19 which is projected on the mount at the bottom and inserted into the depression at the center of the shaft end. In the meantime, driving magnets 5, 7 and motors 6, 8 are rotatably set in cylindrical depressions at the top and the bottom of the tank. Then, 3-phase alternate power is applied from the source 13 through power converter 9 consisting of converter 14 and inverter 15 to drive motors 6, 8 at a desired rotation and rotation torque is given from the magnets 5, 7 through walls 17, 18 to the driving elements 3, 4 to rotate the shaft whereby the culture mixture in the tank is stirred.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Application of the Invention] The present invention relates to a culture device, and particularly to a culture device using a stirring tank driven by a magnet.

[Background of the invention]

A culture device using a magnetically driven stirring tank is, for example,
JP-A-53-66486, JP-A-57-8778
No. 58-116675, Japanese Patent Application Laid-Open No. 1983
Publications such as No. 48071 have been disclosed. This type of device is conventionally known, in which a driven body consisting of a magnet is provided only at the upper or lower part of the stirring shaft, and the driving magnet is rotated by a motor from the outside through the partition wall of the stirring tank.
This rotation causes the internal driven body to rotate, thereby rotating the stirring shaft and the stirring blades attached to this shaft. In this way, when the magnetic force of the driving magnet is applied from the outside, the rotational torque transmitted to the internal driven body is small, leaving the problem that sufficient stirring cannot be achieved. In particular, if a magnetic drive is applied to a stirring tank in a large container store, it will be insufficient.

[Purpose of the invention]

An object of the present invention is to provide a culture apparatus using a magnetically driven stirring tank that can provide sufficient rotational torque.

[Summary of the invention]

The present invention provides a stirring shaft equipped with stirring blades in a stirring tank,
A driven body to which a driving force is applied by a magnet is attached to the upper and lower ends of the stirring shaft, and a bearing that supports the stirring shaft is installed in the stirring tank, and the upper and lower parts of the stirring tank are provided with a driven body. The present invention is characterized in that motors each having a magnet attached to a shaft that provides a driving force to a driven body are arranged, and a power conversion device is provided that supplies common electric power to both motors.

[Embodiments of the invention]

Hereinafter, the present invention will be explained in detail using the drawings.

FIG. 1 is a diagram showing an embodiment of the present invention, and here, an example in which the present invention is implemented in an animal cell culture apparatus is shown. Stirring tank l
A stirring shaft 2 to which a stirring IIt 12 is attached is provided inside. Driven bodies 3 and 4 to which magnets are attached are attached to the upper and lower ends of the stirring shaft 2. As shown in FIG. The stirring shaft 2 is supported by bearings 10 and 19. The bearing lO is attached to a bearing support 11 attached to the inner side wall of the stirring tank 1. Lower bearing 19
is provided in a protrusion shape on the convex bottom surface of the stirring tank 1,
It is fitted into a recess in the center of the shaft 2. The upper and lower parts of the stirring tank 1 form cylindrical recesses, and the driving magnets 5 and 7 are inserted into the recesses.
Each motor 6 and 8 is attached.

Magnets 5 and 7 are attached to the shafts of motors 6 and 8, respectively, and are rotatable. As a result, the rotation torque of the driven bodies 3 and 4 is reduced to the partition wall 1 of the cylindrical recess.
7 and 18 to the respective driven bodies 3 and 4. The motors 6 and 8 have the same specifications, and here a three-phase induction motor m (IM) is used.

Common power is supplied to the motors 6 and 8 from a power conversion device 9. Power conversion on this side! J9 is composed of a converter 14 that converts AC power supplied from a three-phase AC source 13 into DC power, and an inverter 15 that generates AC power having a variable frequency from the DC power converted by the converter 14. There is.

The frequency of the AC power generated by the inverter 15 can be arbitrarily changed by a control command from a rotation speed control section (not shown). As a result, motors 6 and 8 rotate at rotational speeds that correspond to their frequencies. In addition, the above-mentioned motor 6.8
Alternatively, another AC motor may be used, or a DC motor may be used. In this case, it goes without saying that the power converter 9 should be one that is suitable for the motor ξ used.

Now, as mentioned above, the motors 6 and 8 have the same specifications and are configured to be supplied with common electric power, so that they rotate at the same speed with approximately the same torque. Therefore, the transmission torque transmitted to the driven bodies 3 and 4 is almost the same, and there is no torque difference between the upper and lower parts, and the stirring shaft 2
A large transmission torque can be obtained. Furthermore, in this embodiment, cylindrical recesses are provided at the upper and lower parts of the stirring tank l, and torque is transmitted between the driving magnet and the driven body through the partition walls of the recesses, so that both The area facing each other can be increased, and the torque transmission during this area is considerably larger than that of the conventional method.

In this way, the stirring shaft 2 rotates with a large torque, and can maintain the optimum rotation of the stirring blade even if there is a disturbance, and can also change the rotation speed quickly and reliably. can be done. In particular, in the culture of animal cells, the optimal rotation when attaching animal cells to a carrier (e.g., microcarrier), the optimal rotation when suspending culture of a carrier with attached cells, and the separation of cells from the carrier are important. A wide range and accurate speed adjustment is required, such as strong rotation when rotating the motor, but this embodiment can sufficiently cope with this in such applications.

Furthermore, in this embodiment, since the recessed portions at the upper and lower portions of the stirring tank l are inwardly convex, there is no dead space, and sterilization (for example, steam sterilization) essential for this type of use can be completely performed. Furthermore, the stirring tank 1 does not have any penetrating parts, making it easy to maintain sterility after steam sterilization. Of course, it is also suitable for a large-capacity culture device.

〔Effect of the invention〕

As explained above, according to the present invention, sufficient rotational torque can be transmitted to the stirring shaft.

[Brief explanation of the drawing]

FIG. 1 is a diagram showing an embodiment of the present invention.

Claims (1)

[Claims] 1. A stirring shaft with stirring blades attached is provided in the stirring tank, and driven bodies attached to the upper and lower ends of the stirring shaft and driven by magnets, and bearings that support the stirring shaft are installed in the stirring tank. A motor with a magnet attached to the shaft for driving the driven body is installed at the upper and lower part of the stirring tank, and a power converter is installed to supply common power to both motors. A culture device characterized by.