JPS607232B2 - Reaction sample tube automatic transfer analyzer - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a biological fluid analysis device that has sufficient performance as an auxiliary device for a large-scale automatic analyzer when an emergency test is required, and that takes advantage of the features of a simple analyzer. be.

The dramatic advances in modern medicine have drastically changed the format of medical treatment, and the need for so-called clinical test results that analyze changes in the components of a patient's body fluids has increased for diagnosis.

As a result, the number of clinical tests continues to increase year by year, and large hospitals are increasingly introducing automated testing systems that collect the required samples, speed up testing, and save labor in order to process multiple samples and multiple items. The improvement and rationalization of inspection efficiency through automation and mechanization of these inspections has, on the other hand, made the response to emergency inspections insufficient.

Normally, tests using automatic analyzers are conducted intensively, and samples collected at a certain time are often tested the next day, and samples collected on holidays or at night are of course tested on the same day. I can't know the result. Furthermore, if a clinic or other clinic that does not have a testing facility or specialist technicians requests a test from a local testing center, it will take time and date to receive the results. For this reason, there is a need for a large automatic analyzer or an automated simple analyzer that can handle sporadic tests of a small number of samples and multi-item tests for a small number of samples with the same accuracy as the test results of a large automatic analyzer. In the present invention, the temperature is precisely controlled using a reaction sample tube filled with accurately weighed reagents in advance, taking advantage of the characteristics of a simple analyzer that allows anyone to perform tests quickly at any time. A coaxially rotating ring-type reaction sample tube that also serves as a constant temperature bath can be sent to the side light section of a photoelectric colorimeter at an accurate reaction time by controlling the feeding time of the feeding mechanism. It is. In addition, the unit conversion coefficient that also applies to the inspection items is generated as a conversion coefficient signal from a patchboard type conversion coefficient setting unit that can be freely and easily set, including the characteristics of the equipment, in response to the output signal of the item number designation signal storage unit. Values can be created accurately and precisely. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS A specific embodiment of the present invention will be described below in detail with reference to the accompanying drawings.

Figure 1 shows the overall configuration of the automated analyzer using a block diaphragm. It shows a reaction sample tube automatic transfer mechanism using a Kawamani optical system and constant-temperature coaxial rotating ring, and a covered constant-temperature bath 2 and a temperature-controlled chamber. It is controlled by a drive control circuit 16 that controls the coaxial rotation ring in the constant temperature bath 2 and the reaction sample tube transfer mechanism by the hot bath temperature control circuit 14 and the reference time generation circuit 15.

The above-mentioned covered constant temperature bath 2 has a constant temperature bath main body 3 as shown in FIG.
2 and an electric heating lid 33, the thermostatic chamber main body 32
As shown in FIG. 2, the outer circumferential wall 1 of the cylindrical container and the first septum wall B, the first septum wall B and the second septum wall m, and the second septum wall m
Coaxially rotating ring bodies R, , R2 . It is composed of R3. This coaxially rotating ring body has a plurality of recessed holding parts 100 as shown in FIGS. 2 and 7, and has a reaction sample tube 101.
is held and transitioned into an interlude. The heating lid 33 has a color reaction sample tube insertion hole 8 and an enzyme reaction sample tube insertion hole 9 in which heating wires are embedded. 6 is the reaction sample tube supply section;
As shown in the figure, the reaction sample tube 101 is obliquely inclined so that it can be smoothly fed into the recess holding portion 100 on the side of the rotating ring body.

Reference numeral 7 denotes a reaction sample tube take-out section for manually taking out the reaction sample tube 101, which has been sent through the continuous rotation movement of the rotating IJ ring R, in order to add a sample thereto.

The covered fence heating tank 2 consisting of the above-mentioned heating tank main body 32 and electric heating diamond 33 has a visible light range optical system 3 and an ultraviolet light castle optical system 4 that are orthogonal to each other and share the colorimetric light source lamp 52 in the light source chamber 5. It is assembled with.

A coaxial rotating ring R fitted inside the above-mentioned temperature tank main body 32
,, R2, R3 connect the preheating column 102 to the reaction treatment column 103.
It is divided into.

The coaxially rotating ring body R, receives the reaction sample tubes 101 that are next supplied from the reaction sample tube supply unit 6 by the reaction sample tube holder 34 and the pressurizing supply mechanism 35 as shown in FIG. The reagent in the reaction sample tube 101 is heated to the reaction temperature by being taken in and held in the storage position a of the recessed holding part 101 of R, and rotated counterclockwise intermittently according to the rotation control signal, and then moved further. It has a pre-heating train 102 that transfers reaction sample tubes to storage position b within the required heating time determined by the time interval and the number of moving stages. Also, this coaxial rotating ring body R
, has a color reaction processing line 104 that takes in a reaction sample tube 101 pre-humidified and added with a sample from a storage position g of a coaxial rotating ring R2 at a storage position h and transfers it to a storage position i. In addition, reaction sample tube 1 to which a pre-filled sample was added
01 from the enzyme reaction sample tube insertion hole 9 provided in the electric heating lid 33 of the boxed overflow tank 2 at the storage position 1.
It has an enzyme reaction processing column 105 for transferring to. in the end,
The coaxially rotating ring body R has a preheating column 102, a color reaction treatment column 104, and an enzyme reaction treatment column 105.

The coaxially rotating ring body R8 takes in the reaction sample tube 101, which has been preheated and added a sample, from the star-colored reaction sample tube insertion hole 8 of the electric heating lid 33 at the storage position d, and is moved in synchronization with the coaxially rotating ring body R. It has a color reaction treatment column 106 which is rotated clockwise by a storage position interval by a rotation mechanism and transferred to a storage position e after a required time determined by the number of moving stages has elapsed. The coaxially rotating ring body R2 is moved from the storage position f of the reaction sample tube 101 to the storage position e of the coaxially rotating ring body R3 to the reaction sample tube 10.
1 is taken in and rotated counterclockwise by the storage position interval by a rotating mechanism synchronized with the coaxial rotating ring body R, and transferred to the storage position g, where the required time determined by the number of moving stages has elapsed. have

The total number of moving stages possessed by the coaxially rotating ring bodies R, , R2, and R3 is related to the time required for reaction processing, and the reaction sample tubes can be stored at reaction sample tube storage positions equal to the number of moving stages.

The number of moving stages shared by the coaxial rotating rings R, , R2, and R3 is the position e-f for transferring the reaction sample tube between the reaction processing rows.
and g−M. Then, the reaction sample tube 101 in the storage position f, which was transferred by the coaxial rotating ring R, is transferred to the storage position i of the color reaction side light part, and the reaction sample tube 101 is transferred from the storage position m of the enzyme reaction sample tube. The transfer axes E-F and G-A of the transfer mechanism for transferring the enzyme reaction side light part to the storage position m, respectively, are in the radial direction of the coaxial rotating ring body, and the optical axes of the two optical systems are mutually perpendicular to each other, and the colorimetric light source lamp 52 By searching for a configuration that allows them to be shared, the storage position j of the reaction sample tube and the storage position m of the enzyme reaction sample tube become phased at 90 degrees with respect to the rotation axis on the circumference of the coaxially rotating ring body R. , the number of equally spaced storage positions is the division number N, which is an integral multiple of 4. In addition, the coaxial rotating ring body R2,
The storage position arrangement division numbers N2 and N3 of R3 are not equal to N. When the storage position relationship is rotated synchronously, each coaxial rotating ring body R, , R2, R3 rotates between equal angles, so the outer The transfer distance d that closes the storage position interval between rotating rings is R.....d,>R2......also>R
3...d3.

However, the transfer distance D3 (storage positions e to f) of the reaction sample tube transfer mechanism 38 and the transfer distance D2 of the reaction sample tube transfer mechanism 39
(storage positions g to h) are made equal, coaxial rotating ring R,
, R2, R3's radius r of the rotational driving surface, , ra, r3 and the radius W of the driving wheel that is in contact with the driving surface and rotationally drives the coaxial rotating ring.
, , W2, and W3 are placed in a proportional relationship, it is possible to rotate them synchronously by a single drive source 53 whose drive theory is interlocked.

For example, when the interlocking drive wheels rotate by a transfer distance d,, &, d3 on the circumference of the coaxial rotating ring at the same rotation angle, d
=2 汀r/N From the relationship between R2 and R3, if = original, r2 = r30N2/N3, and if N2/N3 = 2, then [2 = public 3].

In addition, in order to simplify the transfer mechanism in the relationship between the coaxially rotating ring bodies R and R2, it is necessary to use D and two D2, so r, = 33.
Therefore, D=r3.

However, if the drive theory is a gear, and its pitch is P and the number of teeth is n, then = P mountain, and when N and = N2, d. ＝also X performance and adversary d. = P (ratio + n') = P (-+ Buddha/2).

In addition, when D>2・V with respect to the size V of the reaction sample tube, r3>2・V also becomes>4wV/N3, and by placing d8 in a proportional relationship with P・n, other values can be determined. can.

As shown in FIGS. 3 and 6, the reaction sample tube transfer mechanism uses a vertical portion of an inverted L-shaped transfer fitting 37 whose horizontal portion is a linear gear plate meshing with a transfer gear 58 connected to a transfer drive motor 57. The first septum wall of the tank body 32 has a coaxially rotating ring body R,
It is placed in a buried position so as not to interfere with the rotation of the body.

Then, when the reaction sample tube 101 is transferred by the intermittent rotation of the coaxial rotating ring body R and comes to rest at the storage position b, the inverted L-shaped transfer fitting 37 is driven by the rotation of the transfer drive motor 67 to take the reaction sample tube 101 to the removal position C. The coaxially rotating ring body R is configured to be transferred to the original position and returned to its original position while the coaxially rotating ring body R is at rest. The reaction sample tube 101' placed at the take-out position C is lifted to the top of the covered overflow tank 2 by a lifter 65 of a rack and pinion mechanism provided at the bottom of the take-out part C in order to add the sample. be. The aforementioned transport mechanism 3
Reference numerals 8 and 39 are constructed in the same manner as the transfer mechanism of the inverted L-shaped transfer fitting 37, and the horizontal portion of each transfer fitting has a linear gear cutting plate that meshes with the transfer gear 58, and the vertical portion of the transfer fitting has the inner circumferential wall W and the second septum wall. The countersunk is located in a buried position so as not to interfere with the rotation of the coaxially rotating ring bodies R3 and R2. The transfer mechanism 38, which is driven simultaneously with the transfer fitting 37 by the rotation of the transfer drive motor 57, transfers the reaction sample tube 101 from the storage position e to the storage position f. The transfer mechanism 39 also includes a reaction sample tube 10.
1 from storage position g to storage position h and coaxially rotate IJ.
During the period of rest of the holding bodies R3 and R2, they return to their original buried position. As shown in FIGS. 2 and 3, the reaction sample tube transfer mechanism 47 has a neck that passes through the beam part n of the ultraviolet optical system 4 and slides in the radial direction G of a coaxially rotating ring body. . This is an inverted L-shaped transfer fitting attached to the top of the sampling block 46 that holds the optical system inspection samples r and S. The vertical part of the transfer fitting 47 is located at a buried position 47 formed so as not to impede the rotation of the first septal wall coaxially rotating ring body R.
’. When the reaction sample tube 101 is transferred from the storage position 1 to the storage position m and comes to rest, the sampling block 46 is rotated by the transfer drive motor 59 of the transfer gear 62 that meshes with the linear gear cutting plate 63 provided at the bottom. The reaction sample tube 101 is held by the vertical portion of the sliding metal fitting 47 and transferred to the storage light section n, and then transferred to the discharge position 0 of the reaction sample tube discharge section 11 after being exposed to the neck.

The reaction sample tube discharge section 11 described above is a constant temperature block in which heating wires are buried, and the optical system side light section is kept at a constant temperature. The reaction sample tube 101 at the discharge position 0 is discharged toward the position i by being driven in the direction of the mark, and after discharge, the discharge member 49 returns to its original position.

Incidentally, when the reaction sample tube 101 is ejected, the sampling block 46 transfers the side light system inspection sample r to the side light section n in order to check the reference value of the fishing light system in the process of returning the vertical part of the transfer fitting 47 to the buried position 47'. Make it pause.

On the other hand, the side light inspection sample S is configured such that the vertical part of the transfer metal fitting 47 is located at the buried position 47' and located at the side light section n. These operations are performed at the timing of two resting periods of the rotation time of the coaxially rotating ring body in order to measure the enzyme reaction. The reaction sample tube transfer mechanism 41 has the same configuration as the transfer mechanism 47 described above, and its operation is such that the sample block 40 holding the measurement system inspection samples P and Q for color reaction measurement rotates between the coaxial rotating rings. It ends by being driven and controlled during a stationary period of time.

The respective reaction sample tubes 101 transferred by the transfer mechanisms 41 and 47 to the storage position i, which is the frequent light section of the visible light optical system 3, and the leakage light section n of the ultraviolet light range optical system 4, are moved to the side light position when exposed to the side light. It is necessary to position it accurately.

Particularly in the case of a round reaction sample tube, the positional deviation of the side light will cause an error in the measured value due to the light-gathering property of the tube due to its round shape. For this purpose, the drive solenoid 61 connected to the reaction sample tube fixture 8 is controlled to move the fixture 48 and the neck. The reaction sample tube 101 transferred to the light part n is sandwiched together with the vertical part of the transfer fitting to be accurately fixed and fixed at the side light position. In addition, 42 is a reaction sample tube fixing fitting which has the same structure as the above-mentioned fixing fitting 48, the other 10 is a reaction sample tube discharge part which has the same structure as the reaction sample tube discharge part 11, and 43 is a reaction sample tube discharge part. Reaction sample tube discharge member 4
It has the same configuration as 9. In addition, 54, 55, 5 in the figure
Numeral 6 is a gear for rotating the coaxially rotating ring bodies R, , R2, and R3, and is driven and rotated via a worm. Next, matters involved in analysis using the above-mentioned mechanism will be explained.

When performing a multi-item analysis test (usually 4 to 8 items) on a specimen of biological body fluid such as blood, a reaction product in which reagents for the test items to be tested are accurately weighed and filled in advance according to the test sheet with the specimen number written on it. Test item names are written on sample tubes 101 and prepared for each sample number.

Then, the reaction sample tubes 101 are stored in a reaction sample tube holder 34 designed to accommodate 8 to 10 reaction sample tubes 101 and prepared. Then, the reaction sample tube holder 34 is attached to the reaction sample tube supply section 6 as shown in FIGS. 2 and 4. On the other hand, the pressure supply mechanism 3
5 is a coaxially rotating ring body R, which is driven by a drive motor 36 whose rotation is controlled by a supply control signal generated from a drive control circuit 16 during a period when the rotation is stationary, and moves the reaction sample tube 101 from position U to the coaxially rotating ring body. It is pushed into the storage position a forming the preheating row 102 of R, and returns to its original position in the next step.

By repeating this operation, the reaction sample tubes 101 are sequentially supplied to the storage position a. In addition, for testing multiple samples for the same item, the preparation and operation are the same as described above, except that the test reagents are the same.

The reaction sample tube 101 sent to the storage position a is heated to the reaction temperature while being transferred intermittently by the coaxial rotating ring R, and is brought to the retrieval position C by the inverted L-shaped transfer fitting 37 at the storage position b. being pushed out.

At this take-out position C, it is pushed upward by the lifter 65. The tester then manually transfers the sample to an automatic sample separator (not shown) and adds a sample thereto, or adds the sample using a manual separator (not shown). In addition, centrifugation and other processes are performed manually. The amount of sample to be added can be determined by checking the item name displayed on the reaction sample tube 101 and pressing the corresponding item name switch from the item name switch group 20 of the sample item number signal generator 17 forming the switch board. Display A
It is configured to be displayed in

When the item name switch is pressed to designate an item name, the sample item number generation unit 17 replaces the item name and generates an item number designation signal. The specimen number is displayed on display B of the display board 19 by pressing the numerical switch 18, and a specimen number signal is generated. This will be called "registration". In this manner, the sample number and sample item are specified using the switch board, and the reaction sample tube 101 for the star color reaction measurement item to which the sample is added is placed on the blocking plate 66 of the star color reaction sample tube insertion hole 8.

Further, the reaction sample tube 101 for enzyme reaction measurement is placed on a blocking plate (not shown) (same as the blocking plate 66) of the insertion hole 9. Therefore, the blocking plate (not shown) of the insertion hole 9 is a coaxially rotating ring body R.
, the reaction sample tube 10 is opened in synchronization with the rotating stationary period.
1 is placed in the storage position d of the coaxial rotary ring body R3 and the storage position i of the coaxial rotary ring body R, either manually or by using the automatic pusher 68, and sends a storage signal to the storage transfer section 21. The storage transfer unit 21 stores item number designation signal storage addresses that are equal to the number of movement stages required for star color reaction processing of the reaction processing rows of the coaxially rotating ring bodies R, , R2, R3 and the number of movement stages necessary for enzyme reaction processing. , have the same number of sample number instruction signal storage addresses, and upon receiving the storage signal, store and save the sample number instruction signal and item number designation signal of the sample item number generation section 17 at their respective first storage addresses. Then, the reaction sample tubes 101 are sequentially moved from one storage position to another by a storage transfer signal generated in synchronization with the inter-rotation control signal of the coaxially rotating ring body, and at the same time are sent to the adjacent storage address, and the reaction sample tubes are transferred to the optical system measuring section. It is retrieved from the last storage address when the data is transferred to the address. The item number designation signal is sent to the coefficient setting section 22, the measurement wavelength selection section 23, the arithmetic processing section 28, and the temporary storage circuit of the display storage circuit 29. Further, the sample number instruction signal is sent to the display storage circuit 29. The coefficient setting section 22 is a matrix patch board using insertion pins, and unit conversion coefficients preset for each inspection item can be easily set by inserting the pins of the patch board.

The horizontal rows of the patch board are connected to output terminals of a line decoder which inputs an item number signal assigned to the inspection item name and rearranges the items into serial numbers. The columns are connected in correspondence with the 3-digit BCD code output terminal of the coefficient signal generation circuit 26. That is, there are 12 pin positions in the column, separated by 4 pins from the bottom, and 3 digits are taken, the scale is x21, Y=lo, Z=100, and the weight of the numbers is a=1, b=2, C. =4, d=8 (a
x, bx, CX, dX) (aY, bYCY, dY) (a
z, b2, Cz, dz).

Then, as a conversion factor that differs for each inspection item, for example, 4
×1,5×10,7×100 is (cx
) (aY, cY) Insert pins into (a2, b2, c2) and set. As a result, the unit conversion factor can be up to 1665 with 1 binary code and 1 place value.

This coefficient signal is switched on only at the pin insertion position when the output signal of the line decoder is added to the relevant item column in the horizontal row, and is applied to the coefficient multiplier circuit 25 as a coefficient setting signal. The measurement wavelength selection section 23 adds the serial number signal corresponding to the inspection item outputted from the line decoder of the coefficient setting section 22 to the multiplexer, and integrates the inspection items having the same measurement wavelength among the determined measurement wavelengths. Create a measurement wavelength selection signal.

Then, it is sent to an optical filter selection circuit (not shown) and compared with the filter number signal from the reaction side light section, and when a match is made, the filter selection signal generated controls the filter selection mechanism and fixes the filter of the measurement wavelength on the optical axis. do. The filter selection mechanism includes a filter block 44 consisting of a disk as the visible light castle optical system 3 on which 4 to 8 types of optical filters are arranged and whose filter numbers can be optically read, and a storage case formed to rotate the disk. and a rotary drive motor 45.

The filter selection mechanism of the ultraviolet optical system 4 for measuring enzyme reactions consists of a slide plate on which two to three types of optical filters are arranged and the filter number can be read optically, and a slide plate that is moved vertically. The filter block 50 is made up of a housing case, and a filter drive motor 51. The optical system uses a measurement wavelength filter selected from the optical filter group by a filter selection signal and fixed to the optical axis to side-light the reaction sample tube in the spotlight section. Then, an electric signal proportional to the transmittance of the star color reaction is output from the photodetector 12, and for enzymatic reaction measurement, it is output from the photodetector 13 and added to the intensification conversion circuit 24 for direct concentration reading, so Lambert-Bell law applies. After converting it into an absorbance electric signal according to the following, it is sent to the unit conversion coefficient multiplication circuit 25.

The coefficient multiplier circuit 25 is configured such that the output voltage Vo of the operational multiplier is input to the input voltage yi, the feedback resistor R2, and the attached resistor R. In the relationship, Vo=1(Vi)R2/R, The following principle is applied.

That is, R is connected to a group of resistors and a semiconductor switch provided corresponding to the coefficient signal.

Then, the semiconductor switch is operated by the coefficient setting signal to create a resistance value corresponding to the conversion coefficient set for each inspection item, and R, /R2, which is equivalent to the voltage increase rate, is changed to convert the absorbance electrical signal input into a concentration direct reading electrical signal output. Convert. Then, in order to record the analog concentration direct electric signal on a data sheet, it is digitized in the A-D conversion circuit 27 and displayed on a numerical display together with the test item name number and specimen number for reading and writing, or the display and recording circuit 29 allows the digital printer to print and record the data. However, in enzymatic reaction measurement, in order to measure the initial rate of reaction, measurements are taken intermittently according to the measurement time interval from the time when the reaction sample tube 101 is transferred to the side light part of the optical system, and the measured values are digitized by the A-D conversion circuit 27. After determining the value, the amount of change per unit time is calculated while being stored in the temporary storage circuit of the arithmetic processing unit 28, and the linearity of the amount of change in reaction rate is determined.

Then, the enzyme unit is determined from the linear variation range, the sample number is displayed on the numerical display together with the test item name, and the data is printed and recorded on the data sheet.

The initial velocity reaction process is also designed to record an analog electrical signal on the pen-writing recorder 31. The contents of the present invention have been described in detail above, but the configuration will be briefly divided and summarized. 1
The reaction sample tube automatic transfer device can use reaction sample tubes in which the diameter of the round tube is equal to the side length of the square tube.

Then, a reaction sample tube 101 is used, which is filled with reagents for test items that have been accurately weighed in advance using a suitable dispenser, and in which the item names are clearly marked. 2. A reaction sample tube holder 34 is used to prepare the reaction sample tube 101 filled with the test reagent in accordance with the sample number on the test list.

3. The reaction sample tube supply unit 6 has a reaction sample tube supply mechanism that sequentially supplies the reaction sample tubes 101 to the preheating row in the coaxially rotating ring body in the temperature tank by attaching a holder.

4 The constant temperature chamber has the shape of a cylindrical container with a lid, and holds a coaxial rotating ring body at a constant temperature in a groove made by an outer circumferential wall, a septum wall, and an inner circumferential wall in which heating wires are embedded, and performs intermittent rotation by a drive signal from a drive control circuit. It has a rotational drive mechanism that closes.

5 The reaction sample tube 101 in the preheating row 102 is heated to the reaction temperature after the required time according to the inter-rotation time of the coaxial rotating ring body R and the number of transfer stages, and then transferred to the extraction position C for sample addition. It is pushed out by the riser 65.

6. The amount of sample added is displayed on the sample amount display by pressing the corresponding item switch on the sample item number signal generator.

The tester either dispenses and adds the sample using a manual dispenser according to the indicated amount, or transfers the reaction sample tube 101 to an automatic dispenser that is activated by a sample amount signal and automatically dispenses and adds the sample amount. Has a display function. Incidentally, mixing by inversion and centrifugation are performed manually.

7. Consists of a numeric switch for specifying and registering the sample number when placing the reaction sample tube 101 containing the sample into the insertion holes 8 and 9 of the reaction side light section, and an item name switch group 20 for specifying and registering the test item name. It constitutes a sample item number signal generation section which is a switch board.

8 A blocking plate 66 is provided in the reaction sample tube insertion holes 8 and 9, and is opened during the rest period of the coaxially rotating ring body, and generates an insertion signal when the reaction sample tube 101 is inserted into the storage position of the coaxially rotating ring body. Has a plate opening/closing function.

9 In response to the insertion signal, the sample number signal and sample item number signal generated by the sample item number signal generation unit are stored in the memory address, and at the same time as the reaction sample tube 101 is transferred by the coaxial rotating ring body, they are stored in the adjacent memory address. Color reaction processing columns 104 and 10 function to sequentially transfer memory signals so that when the reaction sample tube 101 is transferred to the optical side light section, it is taken out from the last address.
6, 107 and an enzyme reaction processing column 105, the storage transfer unit includes a sample number signal storage element group and a test item number signal storage element group.

10 In order to set the conversion factor for converting the absorbance measurement value to the concentration of the test substance, to correct the correlation with the measurement value owned by the testing laboratory, and to facilitate the correction of machine-to-machine errors, BCD code (binary 1G Hayabusa code)
Using a pin insertion type patch board with pin insertion positions for setting 3-digit coefficients and test item names arranged in horizontal rows, by inserting pins according to the pre-calculated coefficients of the test item, the test item can be set. It has a coefficient setting section 22 which receives the item number signal and generates a coefficient setting signal.

11 A transfer mechanism is provided for transferring the reaction sample tube 101 to the reaction processing column of another coaxially rotating ring when the reaction sample tube 101 in the coaxially rotating ring has reached the number of reaction processing columns owned by the coaxially rotating ring.

12 Reaction sample tube 101 after a predetermined reaction treatment time
The optical system neck.

The neck is transferred to the light department. The optical part has a transport mechanism consisting of a sample block that holds the inspection sample. 13. A spotlight position fixing mechanism is provided for accurately fixing the reaction sample tube at a sidelight position in order to eliminate measurement errors caused by deviations in the luminous calendar of the reaction sample tube 101 transferred to the spotlight section.

14. A discharge member is provided which transfers the reaction sample tube that has been exposed to light to the discharge section using the transfer mechanism described on page IZ and then drives it in a semicircular manner to discharge the reaction sample tube out of the reaction side light section.

15 The item name signal converted in response to the item number signal on page 10 is integrated into the same measurement wavelength specified for the inspection item, generates a measurement wavelength selection signal, selects the optical filter of the measurement wavelength from the optical filter group, and selects the optical filter with the measurement wavelength. It has a measurement wavelength selection section that drives and controls a strongly fixed optical filter selection mechanism.

16 A coefficient multiplication circuit that changes the intensification rate of the arithmetic intensifier according to the coefficient setting signal to directly read the concentration of the test substance in units, an A-D conversion circuit that digitizes the concentration direct reading, and a converter that converts the measured value and sample number. It has a display/recording circuit that displays the item name and prints and records it on the data sheet.

17 It has an arithmetic processing unit that measures the initial reaction rate for enzyme reaction measurement, tests linearity of reaction rate, determines the linear range, calculates the unit time amount, and calculates the enzyme unit.

Incidentally, the measured value is printed and recorded on a numerical display and data sheet together with the specimen number and test item name in the display recording circuit.

The reaction speed can be obtained by recording an analog signal with a pen counter, or by directly recording numerically measured values measured at equal intervals on a digital printer. Next, pages 18 and 19 also describe embodiments of the present invention.

18 A sample tube holder 34 into which a round or square reaction sample tube 101 filled with accurately weighed reagents is inserted, an accurately temperature-controlled temperature tank equipped with a mechanism for sequentially supplying sample tubes 101, and a thermostat A rotating heating ring that rotates in response to a drive control signal in a tank and overflows sample tubes and sequentially sends them out from the sample dispensing section, and a striped ring that is coaxial with a striped ring that has a sample tube insertion hole specified by the inspection item. a rotating reaction ring body;
A photoelectric colorimetric unit consists of two types of optical systems that share a light source, divide the measurement wavelength range into visible light and ultraviolet light, and are equipped with optical filters and photodetectors, arranged at right angles to each other. It consists of a transfer mechanism that has a check sample that allows inspection and adjustment of the optical system at the same time during the process of transporting the sample tube, side-lighting the rotating ring body with the side light part of the optical system, and discharging it to the outside. A coaxial rotating ring-type reaction sample tube automatic transfer analyzer that has two measurement series and mutually orthogonal optical systems that discharge continuously.

19 In the above 180%, there is a sample item number signal generation unit consisting of a switch board that generates the reception number of the sample to be tested as a sample number designation signal and the test item name as an item number designation signal, and a position signal of a coaxial rotating ring. A storage transfer unit is configured to sequentially transfer the storage signal at a storage address to the next storage address and output from the final storage address, and an item number is configured to convert the photodetector output of the photoelectric colorimetric unit into concentration and then convert it into units. A coefficient setting section consisting of a patch board that receives a signal and generates a conversion coefficient signal; and a measurement wavelength selection section that selects a measurement wavelength from a group of optical filters and fixes it on the optical axis based on an item number signal from the final storage address. A reaction sample tube automatic transfer analyzer comprising a circuit designed to digitize the direct reading of the concentration value, display it on a numerical display together with the sample number and test item name, and print it on the data sheet in green.

Since the present invention has the structure and operation described above, it has the following effects. ■ Take out the reaction sample tube IQI, which has been sent from the pre-warming column 102 and has been filled with the reagent for the test item, from the reaction sample extraction section 7.
Simply dispense the sample and insert it into the color reaction sample tube insertion hole 8 and the enzyme reaction sample tube insertion hole 9, respectively.
It is possible to easily measure substances within the body automatically, quickly and with high accuracy.

■ Since the colorimetric light source lamp 52 is shared and divided into the visible light range optical system 3 and the ultraviolet light range optical system 4 which are orthogonal to each other, the measurement level accuracy for each optical system against power fluctuations etc. is the same, resulting in improved measurement accuracy. Unbalance does not occur, and the number of parts can be reduced to achieve compactness.

[Brief explanation of drawings]

Fig. 1 is an overall block diagram of the device of the present invention, Fig. 2 is a schematic plan view mainly showing the constant coaxial rotating ring body portion of the two optical systems in the device of the present invention, and Fig. 3 is the same as that of Fig. 2. 4 is a schematic longitudinal side view of the reaction sample tube holder, Fig. 5 is a schematic longitudinal side view of the reaction sample tube pusher, and Fig. 6 is a schematic longitudinal side view of the reaction sample tube holder. FIG. 7 is a schematic perspective view of the coaxial rotating ring body, FIG. 8 is a perspective view of the reaction sample tube discharge section, FIG. 9 is a perspective view of the reaction sample fixing fitting, and FIG. 10 is a schematic vertical side view of the upper device. is a plane diagram of FIG. 9 for explaining the principle. R,,R2,R3..."Coaxial rotating ring body, 101...
... Reaction sample tube, 102 ... Prewarming column,
105... Enzyme reaction treatment column, 106, 107.
...Color reaction treatment column. Figure* Next 2 Figures Next 4 Figures Next 5 Figures of scales* Figures of scales

Claims (1)

[Scope of Claims] 1 (a) A reaction sample tube 101 filled in advance with a reagent for the test item to be tested is housed in the reaction sample tube supply section 6, while (b) A concentric circle of the thermostatic chamber body 32 The coaxially rotating ring bodies R_1, R_2, and R_3 are provided to rotate intermittently, and the coaxially rotating ring body R_1 is divided into a preheating column 102, an enzyme reaction treatment column 105, and a coloring reaction treatment column 104, and the preheating A reaction sample tube take-out section 7 is provided at the end of the temperature column 102, a color reaction treatment column 107 is provided on the coaxially rotating ring body R_2, and a color reaction treatment column 106 is provided on the coaxially rotating ring body R_3. (c) A part of the reaction sample tube supply section 6 is communicated with the preheating row 102 of the coaxially rotating ring body R_1. (c) A coaxially rotating ring body that communicates with the coloring reaction sample tube insertion hole 8. The color reaction treatment row 106 of R_3 and the color reaction treatment row 107 of the coaxially rotating ring body R_2 are to be communicated, and the color reaction treatment row 107 and the color reaction treatment row 107 of the coaxially rotating ring body R_1 are connected. (e) Furthermore, the enzyme reaction sample tube insertion hole 9 is to be communicated with the enzyme reaction treatment column 105 of the coaxially rotating ring body R_1. (f) The colorimetric light source lamp 52 is shared. It is divided into a visible light range optical system 3 and an ultraviolet light range optical system 4 that are perpendicular to each other, and the visible light range optical system 3 allows the light to move from the coloring reaction processing column 104 of the coaxially rotating ring body R_1. The reaction sample tube 101
The tube is configured to measure the substance in the reaction sample tube 101 that has been moved from the enzyme reaction processing column 105 of the coaxially rotating ring body R_1 by the ultraviolet light region optical system 4. Automatic reaction sample tube transfer analyzer. 2. (a) A reaction sample tube section 101 filled in advance with a reagent for the test item to be tested is housed in the reaction sample tube supply section 6, while (b) A coaxially rotating ring body is arranged on the concentric circle of the constant temperature chamber body 32. R_1, R_2, and R_3 are provided to rotate intermittently, and the coaxially rotating ring body R_1 is divided into a preheating row 102, an enzyme reaction treatment row 105, and a coloring reaction treatment row 104, and the ends of the preheating row 102 are separated. A reaction sample tube take-out part 7 is provided in the coaxially rotating ring body R_2, and a coloring reaction processing column 107 is provided in the coaxially rotating ring body R_3, and a coloring reaction processing column 106 is further provided in the coaxially rotating ring body R_3, and (c) the above-mentioned A part of the reaction sample tube supply section 6 is connected to the preheating row 102 of the coaxially rotating ring body R_1 (d) A coaxially rotating section that communicates with the storage position d positioned in the coloring reaction sample tube insertion tube 8. The coloring reaction treatment column 106 of the ring body R_3 and the coloring reaction treatment column 107 of the coaxially rotating ring body R_2 are to be communicated, and furthermore, the coloring reaction treating column 107 and the coloring reaction treatment column 107 of the coaxially rotating ring body R_1 are connected. (e) Furthermore, the enzyme reaction treatment column 105 of the coaxially rotating ring body R_1 is communicated with the storage position 1 located in the enzyme reaction sample tube insertion hole 9; (f) the storage position d, 1 to the storage transfer unit 21, and the storage transfer unit 21 sends the sample number designation signal of the sample item number generation unit 17 and the item number designation signal selected from the item name switch group 20 to the first one, respectively. While saving at the memory address (g
) The colorimetric light source lamp 52 is divided into a visible light range optical system 3 and an ultraviolet light range optical system 4 that are shared and orthogonal to each other,
The color reaction treatment row 104 of the coaxial rotating ring R_1
When the reaction sample tube 101, which had been moved from 101 is transferred to the ultraviolet optical system 4, the item number designation signal taken out from the final storage address of the storage transfer section 21 is sent to the coefficient setting section 22, and the output from the coefficient setting section 22 is The signal is output to the coefficient multiplication circuit 25 as a coefficient setting signal, and at the same time, the light transmitted through the reaction sample tube 101 is photoelectrically converted and added to the coefficient multiplication circuit 25, and further, the A-D conversion circuit 27 converts the measured value. Reaction sample tube automatic transfer analyzer designed for display.