JPS6065285A - Pulsation eliminating apparatus - Google Patents
Pulsation eliminating apparatusInfo
- Publication number
- JPS6065285A JPS6065285A JP17311383A JP17311383A JPS6065285A JP S6065285 A JPS6065285 A JP S6065285A JP 17311383 A JP17311383 A JP 17311383A JP 17311383 A JP17311383 A JP 17311383A JP S6065285 A JPS6065285 A JP S6065285A
- Authority
- JP
- Japan
- Prior art keywords
- liquid
- pulsation
- flow
- discharge nozzle
- under pressure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
- F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
- F04B11/00—Equalisation of pulses, e.g. by use of air vessels; Counteracting cavitation
- F04B11/0008—Equalisation of pulses, e.g. by use of air vessels; Counteracting cavitation using accumulators
- F04B11/0016—Equalisation of pulses, e.g. by use of air vessels; Counteracting cavitation using accumulators with a fluid spring
- F04B11/0025—Equalisation of pulses, e.g. by use of air vessels; Counteracting cavitation using accumulators with a fluid spring the spring fluid being in direct contact with the pumped fluid
Abstract
Description
【発明の詳細な説明】
〔発明の技術分野〕
この発明は、微少流体の圧送に際し生ずる微少流体の脈
動を除去する脈動除去装置忙関する。DETAILED DESCRIPTION OF THE INVENTION [Technical Field of the Invention] The present invention relates to a pulsation eliminating device that eliminates pulsation of a microfluid that occurs when the microfluid is pumped.
従来、フルオロサイトメトリーあるいは血球等の粒子カ
ウンタにおけるシースフロ一方式採用の微少流体輸送装
置は、第１図に示すように、第１の液体１を吐出する吐
出ノズル２を、第２の液体６の流路管４内に配置し、前
記吐出ノズル２″を、図示しないパイプを介して、第１
のシリンジポンプ５に接続し、前記流路管４を、図示し
ないパイプを介して、第２のシリンジポンプ６に接続し
、そして、前記第１および第２のシリンジポンプ５．６
を駆動するシリンジ駆動機構７を備え、前記シリンジ駆
動機構７により前記第１および第２のシリンジポンプ５
．６を駆動することにより、圧送される第２の液体６中
に、前記吐出ノズル２より前記第１の液体１を吐出して
、第２の液体乙に囲繞されて圧送される第１の液体１の
層流を形成するように構成されている。前記微少流体輸
送装置において、前記第１の流体１と前記第２の流体６
とを圧送する流路管８の内法を、たとえば２５０×２５
０μｍにし、前記吐出ノズル２の先端部の内径を、たと
えば３００μｍＫすると、第１の液体１および第２の流
体乙の流速により、たとえば径３０μｍの第１の液体１
の層流が流路管８内で形成される。Conventionally, a microfluidic transport device employing a sheath-flow type in particle counters such as fluorocytometry or blood cells, as shown in FIG. The discharge nozzle 2'' is arranged in the flow path pipe 4, and the discharge nozzle 2'' is connected to the first
The flow pipe 4 is connected to a second syringe pump 6 via a pipe (not shown), and the first and second syringe pumps 5.6
The syringe drive mechanism 7 drives the first and second syringe pumps 5.
．． 6, the first liquid 1 is discharged from the discharge nozzle 2 into the second liquid 6 to be pumped, and the first liquid is surrounded by the second liquid B and is pumped. It is configured to form a laminar flow of 1. In the microfluid transport device, the first fluid 1 and the second fluid 6
For example, the inner diameter of the flow path pipe 8 for conveying under pressure is 250×25
0 μm and the inner diameter of the tip of the discharge nozzle 2 is, for example, 300 μmK, the flow rate of the first liquid 1 and the second fluid B causes the first liquid 1 to have a diameter of 30 μm, for example.
A laminar flow of is formed in the flow pipe 8.
しかしながら、第２のシリンジポンプ６で第２の液体３
を圧送すると、第２図に示すように、液圧が脈動し、そ
の結果、第６図に示すように、第１の液体１の径が変化
し、流量にゆらぎが生ずる。However, with the second syringe pump 6 the second liquid 3
When the first liquid 1 is pumped, the liquid pressure pulsates as shown in FIG. 2, and as a result, the diameter of the first liquid 1 changes as shown in FIG. 6, causing fluctuations in the flow rate.
そうすると、前記流路管８内の第１の液体またとえばサ
ンプルの径を厳密に管理して、流路管８内の第１の液体
に光を横断、通過させてサンプル中の粒子をカウントし
ようとする場合、サンプルの径が脈動することにより、
正確に粒子な計数することができな（なる。Then, by strictly controlling the diameter of the first liquid in the flow path tube 8 or the sample, let light cross and pass through the first liquid in the flow path tube 8 to count particles in the sample. When the diameter of the sample pulsates,
It is not possible to accurately count particles.
この発明は前記事情に基づいてなされたものであり、吐
出ノズルより吐出する第１の液体を、シリンジポンプで
圧送する第２の液体で囲繞して、微少流径な有する第１
の液体の層流を形成１−るために、第１の液体の液圧の
脈動を除去する脈動除去装置を提供することを目的とす
るものである。This invention was made based on the above circumstances, and the first liquid discharged from the discharge nozzle is surrounded by the second liquid pumped by a syringe pump, and the first liquid has a minute flow diameter.
An object of the present invention is to provide a pulsation eliminating device that eliminates pulsations in the hydraulic pressure of a first liquid in order to form a laminar flow of a first liquid.
前記目的を達成するためのこの発明の概要は。 The outline of this invention for achieving the above object is as follows.
吐出ノズルより吐出する第１の液体の流れを囲繞するた
めの第２の液体をシリンジポンプから前記吐出ノズルに
まで圧送する流路管にＲＲ曳札翫太共く、第２の液体の
液圧を吸収する緩衝部材な設けてなることな特徴とする
ものである。The hydraulic pressure of the second liquid is connected to the flow path pipe that pressure-feeds the second liquid from the syringe pump to the discharge nozzle to surround the flow of the first liquid discharged from the discharge nozzle. It is characterized by the provision of a shock absorbing member.
次に、この発明の一実施例について説明する。 Next, one embodiment of the present invention will be described.
この発明の一実施例である脈動除去装置９は、第４図に
示すように微少流体輸送装置における流路管４において
、吐出ノズル２により第１の液体１を第２の液体乙に吐
出する以前の適宜の部位に設け、流路管４に、コネクク
部材（図示せず。）を介して、有底の筒体１０を結合し
、前記筒体１０内に緩衝部材として気体１またとえば空
気を封入してなる。第４図においては、ｎ１記脈動除去
装置９を、吐出ノズル２を流路管４に挿入する以前の流
路管４部分に設けている。ま１こ、第４図において、第
１図に示すのと同一の機能を有する部材については同一
の番号を付して、その詳細な説明を省略する。As shown in FIG. 4, a pulsation removal device 9, which is an embodiment of the present invention, discharges a first liquid 1 into a second liquid B through a discharge nozzle 2 in a flow path pipe 4 in a microfluid transport device. A cylindrical body 10 with a bottom is connected to the flow path pipe 4 via a connecting member (not shown), and a gas 1 or air, for example, is inserted into the cylindrical body 10 as a buffer member. It will be enclosed. In FIG. 4, the n1 pulsation removing device 9 is provided in a portion of the flow pipe 4 before the discharge nozzle 2 is inserted into the flow pipe 4. Also, in FIG. 4, members having the same functions as those shown in FIG. 1 are designated by the same numbers, and detailed explanation thereof will be omitted.
このように流路管４に脈動除去装置９な設けると、第２
の液体６０液圧が増加すると筒体１０内の気体１１が圧
縮されることとなるので、脈動除去装置９の取り付は部
分以後の流路管４内の第２の液体乙には、第５図に示す
ように、その脈動が殆んど除去されることとなる。When the pulsation removing device 9 is provided in the flow path pipe 4 in this way, the second
When the liquid pressure of the liquid 60 increases, the gas 11 in the cylinder 10 will be compressed. As shown in FIG. 5, most of the pulsation is eliminated.
以上、この発明の一実施例について説明したが、この発
明は前記実施例に限定されるものではなく、この発明の
妥旨の範囲内で適宜に変形して実施することができるの
はいうまでもない。Although one embodiment of this invention has been described above, it goes without saying that this invention is not limited to the above embodiment and can be implemented with appropriate modifications within the scope of the spirit of this invention. Nor.
脈動除去装置９の取り付は位置は、流路管４における、
吐出ノズル２の先端部以前の適宜の箇所で良く、第６図
に示すように、流路管４の、吐出ノズル２の先端から挿
入部位までの適宜の位置に、脈動除去装置９を取り付け
ても良い。The mounting position of the pulsation remover 9 is in the flow path pipe 4.
The pulsation removing device 9 may be installed at an appropriate position before the tip of the discharge nozzle 2, and as shown in FIG. Also good.
また、脈動除去装置は、第２のシリンジポンプ６で圧送
される第２の液体３の脈圧を有効に吸収することができ
ればよいのであるから、有底の筒体に、圧縮係数が大き
く、かつ、第２の液体３と混和しない緩衝用液体を封入
して構成してもよく、さらに、圧力センサに用いられる
ベローズ、ダイヤフラム等の弾力性部材を用いて構成し
てもよい。In addition, since the pulsation removal device only needs to be able to effectively absorb the pulsation pressure of the second liquid 3 pumped by the second syringe pump 6, the pulsation removal device is made of a cylinder with a bottom and a large compression coefficient. Further, it may be constructed by enclosing a buffering liquid that is immiscible with the second liquid 3, and may also be constructed using an elastic member such as a bellows or a diaphragm used in a pressure sensor.
以上詳述したようにこの発明によると、吐出ノズルより
吐出する第１の液体な、シリンジポンプで圧送する第２
の液体で囲繞しつつ輸送する際、第２の液体の液圧の脈
動を殆んど除去することができるので、第１の液体の流
径を常に一定に保持し、第１の液体の層流な良好に形成
することができる。したがって、この発明の脈動除去装
置を取り付けた微少流体輸送装置をフルオロサイトメト
リーや粒子カウンタに設けると、正確な分析、計数を行
なうことができる。As detailed above, according to the present invention, the first liquid discharged from the discharge nozzle, the second liquid pumped by the syringe pump,
When transporting the second liquid while surrounding it with liquid, it is possible to almost eliminate the pulsation of the liquid pressure of the second liquid, so the flow diameter of the first liquid is always kept constant and the layer of the first liquid is It can be formed easily and smoothly. Therefore, if a microfluid transport device equipped with the pulsation removing device of the present invention is installed in a fluorocytometry or a particle counter, accurate analysis and counting can be performed.
第１図は従来の微少流体輸送装置を示す説明図、第３図
は前記微少流体輸送装置において第１の流体を第２の流
体で囲繞して輸送する状態を示す説明図、第２図は前記
微少流体輸送装置における第２の液体の３棟の液圧とそ
の脈動を示す特性図。
第４図はこの発明の一実施例を示す説明図、第５図は前
記実施例における第２の液体の液圧とその脈Ｓを示す特
性図、および第６図はこの発明の他の実施例を示す説明
図である。
１・・・第１の液体、 ２・・・吐出ノズル、 ３・・
・第２の液体、 ４・・・流路管、 ６・・・シリンジ
ポンプ、１１・・・緩衝部材（気体）。FIG. 1 is an explanatory diagram showing a conventional microfluid transport device, FIG. 3 is an explanatory diagram showing a state in which a first fluid is surrounded by a second fluid and transported in the microfluid transport device, and FIG. FIG. 7 is a characteristic diagram showing the hydraulic pressure of three buildings of the second liquid in the microfluid transport device and its pulsation. FIG. 4 is an explanatory diagram showing one embodiment of the invention, FIG. 5 is a characteristic diagram showing the hydraulic pressure of the second liquid and its pulse S in the embodiment, and FIG. 6 is another embodiment of the invention. It is an explanatory diagram showing an example. 1... First liquid, 2... Discharge nozzle, 3...
- Second liquid, 4... Channel pipe, 6... Syringe pump, 11... Buffer member (gas).
Claims (1)
めの第２の液体をシリンジポンプから前記吐出ノズルに
まで圧送する流路管に設Ｒ％’ｊｌＬ％虻入べ１、第２
の液体の液圧を吸収する緩衝部材を設り−でなることを
特徴とする脈動除去装置。A second liquid for surrounding the flow of the first liquid to be discharged from the discharge nozzle is installed in a flow path pipe that pressure-feeds the second liquid from the syringe pump to the discharge nozzle.
1. A pulsation removal device comprising: a buffer member for absorbing the hydraulic pressure of a liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17311383A JPS6065285A (en) | 1983-09-21 | 1983-09-21 | Pulsation eliminating apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17311383A JPS6065285A (en) | 1983-09-21 | 1983-09-21 | Pulsation eliminating apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6065285A true JPS6065285A (en) | 1985-04-15 |
Family
ID=15954390
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP17311383A Pending JPS6065285A (en) | 1983-09-21 | 1983-09-21 | Pulsation eliminating apparatus |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6065285A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU187572U1 (en) * | 2018-12-13 | 2019-03-12 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Тамбовский государственный технический университет" (ФГБОУ ВО "ТГТУ") | Device for generating pulsating flows in phantoms of blood vessels |
-
1983
- 1983-09-21 JP JP17311383A patent/JPS6065285A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU187572U1 (en) * | 2018-12-13 | 2019-03-12 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Тамбовский государственный технический университет" (ФГБОУ ВО "ТГТУ") | Device for generating pulsating flows in phantoms of blood vessels |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103837462B (en) | A kind of small-sized flow cytometer liquid-way system | |
| US20190154551A1 (en) | Device having a micro fluid actuator | |
| EP3314123B1 (en) | Systems, apparatuses, and methods for cell sorting and flow cytometry | |
| TR200102725T2 (en) | Precision dosing device for liquids. | |
| CN103557143A (en) | Closed loop piezoelectric membrane pump and flow control method thereof | |
| CN203772701U (en) | Miniaturized flow cytometry liquid path system | |
| JPS6065285A (en) | Pulsation eliminating apparatus | |
| CN205581064U (en) | High pressure constant current infusion set | |
| JP2002214214A (en) | Column for high performance liquid chromatography | |
| US5256573A (en) | Method for diluting and mixing liquid specimen | |
| JPS61197778A (en) | Diaphragm pump having recirculation type discharge apparatus | |
| CN106872340A (en) | A kind of liquid fluid system for FCM analysis | |
| GB1045285A (en) | Combination fluid pulsation dampener and separator for removing liquid or other foreign matter from fluids | |
| JPS5667733A (en) | Measuring apparatus for discharge of corpuscle | |
| CN219957486U (en) | Stainless steel capillary chromatographic column filling device | |
| CN206546318U (en) | A kind of liquid fluid system for FCM analysis | |
| CN108300647B (en) | Bubble interceptor | |
| JPS59151040A (en) | Optical scattering type particle counting device | |
| US5018554A (en) | Device for the damping of pumps with intermittent delivery | |
| JPS6226702B2 (en) | ||
| JPS5910064U (en) | Liquid chromatograph liquid delivery device | |
| JP7410495B2 (en) | Gas component detection device | |
| WO1989004961A1 (en) | Hematology cell counting apparatus | |
| JPS58104484U (en) | Pulsation absorption hose | |
| JP2806403B2 (en) | Inspection device |