JPS6041588B2 - Method for promoting changes in biological properties of microorganisms - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for promoting changes in biological properties of microorganisms.

Living organisms in the natural world are classified into microorganisms, plants, and animals, and microorganisms are diverse and have been closely related to human life since ancient times. I have received immeasurable benefits from it.

The brewing industry in the food industry makes effective use of the action of enzymes produced by fungi among microorganisms (especially various yeasts), and in the pharmaceutical industry, bacteria such as actinomycetes are used to Various antibiotics are manufactured.

Currently, the food crisis and resource issues have become a global issue, and the effectiveness of biological resources called biomass has been attracting attention.

Among biomass-related fields, the potential of applied microbial industry is immeasurable, and it has the potential to be a powerful solution to the food and resource problems mentioned above. For example, alcohol fuel obtained by alcoholic fermentation of carbohydrates using yeast is attracting attention as an alternative fuel, and chlorella and hydrogen bacteria are highly efficient and are considered promising sources of various foods and feeds that can be mass-produced. .

Furthermore, the use of microorganisms will increase in the treatment and recycling of industrial wastewater, urban wastewater, and waste.

The present invention provides a powerful means for the development of the microbial industry, and relates to a method for promoting changes in the biological properties of microorganisms.

Traditionally, the brewing and pharmaceutical industries have repeatedly improved yeast and bacteria using physical or chemical methods based on microbial genetics to create useful microorganisms with high yields and high activity. .

For example, as a physical method, the method of inducing artificial mutations by irradiation with X-rays, gamma rays, ultraviolet rays, etc. is widely used, and as a chemical method, it is caused by treatment with specific chemicals. Methods for genetically improving bacterial strains are known. In addition, methods for promoting acquired changes in biological properties include controlling the pH in the culture solution and administering chemical substances to cause chemical effects and changes in the enzymes of microorganisms.
There is a method of changing the biological activity of microorganisms, that is, promoting changes in their biological properties. However, in the case of the above-mentioned various methods, especially the physical method, not only the equipment becomes complicated and expensive, but also the handling thereof is dangerous, making the operation troublesome.

In the case of chemical methods, there are many types of chemicals, processing methods,
It has drawbacks such as it is difficult to set the treatment concentration, etc., and a useful method cannot be obtained without extensive experiments. In contrast, the present invention is a physicochemical method.

That is, the present inventor disclosed a method for promoting and advancing the growth of plants by providing an electric discharge atmosphere to them in a previous application entitled "Method for Promoting Enlargement and Growth of Plants" (Japanese Unexamined Patent Publication No. 55-42512). , the present invention applies the above invention to microorganisms. In other words, due to discharge and various other electromagnetic waves,
By exciting or ionizing a neutral gas, a negative ionic gas is created, and this ionic gas is contained. This method promotes changes in the biological properties of microorganisms by treating them in a warm atmosphere.

The present inventors believe that a discharge atmosphere, that is, an atmosphere containing ionic gases generated by electric discharge, promotes the germination and growth of plants. We assumed that the atmosphere would have various effects on not only plants but also microorganisms, and among microorganisms, we investigated yeast as a representative of fungi, chlorella as a representative of general algae, and Artemia as a representative of protozoa. A systematic experiment was conducted to confirm the effect.

The above experiment will be described in detail below as an example of the implementation procedure of the present invention. First, we conducted two types of experiments in which yeast, which has been attracting attention in recent years for producing alternative fuels through alcoholic fermentation, were treated with an iron-like gas produced by electrical discharge, and we obtained good results. I will explain about this in detail.

D Experimental method When a silent discharge generator is used as the discharge generator (
Experiment A) As shown in Figure 1, dry yeast was placed in a shear dish 1 and placed in a blower tube 2, and the blower tube 2 contained ionic gas produced by discharging it with a silent discharge device E. The atmosphere was supplied by fan 3, and each sample was treated for a predetermined period of time to obtain four types of samples.These were mixed in a culture solution and cultured at 34°C in a constant temperature bath to observe changes in yeast cells over time. was measured.

However, Hennberg's solution was used as the culture solution. The discharge treatment air was generated by a discharge device E shown in FIG.

This discharge device E has piano wires 4 arranged in a cylindrical shape with predetermined intervals, a glass cylinder 5 is wrapped around the outer periphery of the piano wire 4, and a conductive paint coated on the inner peripheral surface of the glass cylinder 5 is used. A negative DC high voltage is applied between the wires 4 to generate linear silent discharge in the gap between the conductive paint and the piano wire 4. Incidentally, grounding is performed by an aluminum box outside the glass cylinder 5. In this way, since metal is used in addition to the grounded glass cylinder 5, spark discharge due to high voltage charging can be prevented. As shown in Figure 1, this discharge atmosphere containing ionic gas is fed into the blower tube 2 by a fan 3, but this discharge atmosphere contains negative oxygen ions, nitrogen ions, etc. It is contained in concentrations thousands to tens of thousands of times higher than that of

When using a negative ion generator as a discharge generator (Experiments B1 and B2) In Experiment B1, dry yeast was placed in the bottom of the flask, and the ion discharge port of the negative ion generator was placed in front of the inlet of the flask. Various samples were prepared by supplying an atmosphere containing negative ionic gas (hereinafter referred to as negative ion treatment), and these samples were cultured in a culture solution at a constant temperature of 34°C.
Changes in the number of yeast cells over time were measured.

In experiment B2, dry yeast was mixed with a culture solution, placed in a flask, placed with the negative ion discharge port of the negative ion generator facing the inlet of the flask, and continuously treated with negative ions.
The yeast cells were cultured at a constant temperature of 4°C, and changes in the number of yeast cells over time were measured.

However, for both Jitsken B1 and Jitsken B2, Henn was used as the culture medium.
Berg's solution was used.

In principle, a negative ion generator is a circuit shown in Figure 3, in which high voltage alternating current is rectified, smoothed by a capacitor C, and a weak current is discharged between the output terminal P and ground by interposing a high resistance R. This generates only negative ionic gas.

(■) Experimental results and study The results of Experiment A are shown in FIGS. 4 to 6, and the results of Experiment B1 and B2 are shown in FIGS. 7 to 10.

Figure 4 shows "changes in cell number over time due to discharge treatment",
Symbols Cl, C2, C3, and C4 indicate those subjected to no treatment, 2 $f discharge treatment, half-time discharge treatment, and n-hour discharge treatment, respectively.

FIG. 5 shows "discharge treatment time and cell life time", and FIG. 6 shows "discharge treatment time and number of days to reach critical value".

Figure 7 shows "changes in cell number over time due to negative ion treatment", where symbols C5, C6, C7, and C8 are untreated and 2$f, respectively.
Shown are those with intermittent negative ion treatment, 4-hour negative ion treatment, and long-time negative ion treatment. FIG. 8 shows "negative ion treatment time and cell generation time", and FIG. 9 shows "negative ion treatment time and number of days to reach critical value". Figure 10 shows "changes over time in cell number during culture with continuous negative ion treatment", with symbols C9 and C
IO indicates those of untreated culture and continuous negative ion treatment culture, respectively. As can be seen from the temporal change in the number of yeast cells shown in FIG. 4 for Experiment A, it can be seen that the growth rate of yeast cells is dramatically accelerated by the discharge treatment.

That is, untreated yeast proliferates relatively slowly and linearly until the fourth day of culture, then gradually proliferates and reaches a nearly critical state on days 6 to 12.

On the other hand, those subjected to the 2@ discharge treatment reached a critical state on the 4th day of culture, and the number of critical cells was also large.

In addition, compared to those subjected to the 24-hour discharge treatment, those treated for an extended period of time and n hours show a lower cell proliferation tendency.

The above-mentioned tendency is remarkable until about the 6th day of culture, and after the 6th day, it reaches a critical value of approximately the same level. As a result, it can be seen that when dry yeast is treated with ionic gas by electric discharge, the highest growth rate can be obtained if the yeast is treated with two bridges.

Here, let us consider the factors that speed up the cell division and proliferation of yeast by the discharge treatment as described above.

As mentioned above, the negative active oxygen ions generated by the discharge work to promote the supply of oxygen to the yeast, and as a result, the oxidation effect, which plays a large role in the yeast metabolism, is promoted, resulting in cell growth. It is estimated that growth is promoted, generation time is shortened, and the frequency of cell division is increased.

Therefore, in order to examine this, the cell generation time is shown in FIG. As can be seen from Figure 5, the generation time of discharge-treated yeast is significantly shorter than that of untreated yeast.
A large difference was observed depending on the discharge treatment time, with the 24-hour treatment having the minimum generation time.

Here, in order to examine whether even the genetic properties of the yeast cells have changed due to the discharge treatment, a critical value of cell number is calculated. Table 1 shows the critical values for the number of cells after 3 weeks from the start of culture. The maximum rate of change in the critical value shown in Table 1 is 2.4%, which is within the relative error of 3.2%, so it can be said that the critical value is approximately constant. Therefore, it is presumed that the genetic properties of the yeast cells were not changed by the discharge treatment.

As described above, it was found that although the discharge treatment shortens the cell generation time, it does not affect the critical value of cell number.

Next, the average value of the critical values in Table 1, 420×8×1CF'
The number of days required to reach number/Tnl is calculated using generation time and is shown in FIG. As can be seen from this figure, the non-treated one reaches the critical value in about 6 days, while the two-washed one reaches the critical value on about the second day, and the period is 6 days.
7% shorter. In the case of 4S1 processing, the period is shortened by 17%, but in the case of n-hour processing,
Almost never shortened. Since the characteristics shown in FIG. 6 are of the same type as those shown in FIG. 5, it is determined that the shortening of the period in which the critical value is reached is a secondary result of the shortening of the generation time.

- Regarding Experiments B1 and B2 - In this experiment, an atmosphere containing a negative ionic gas generated by a negative ion generator was applied to yeast, that is, negative ion treatment was performed.

As can be seen from the change in cell number over time in Figure 7, in untreated yeast, the cell number increases rapidly to some extent until about the 4th day of culture, but then continues to increase slowly until about day 8 to 10. The number of cells in the critical state is approximately 460 x 8 x 1.
C1' pieces/ml.

On the other hand, when treated with negative ions, the rate of cell division and proliferation is significantly increased. In the case of the two-hour treatment, this tendency was not remarkable, but in the case of the two-hour treatment and the n-hour treatment, a rapid increase in cell number occurred until the third day, and reached an almost critical state on the fourth day. After that, the critical state is maintained.

In the case of negative ion treatment, unlike the case of discharge treatment, the cell proliferation rate increases as the treatment time increases.

Therefore, it can be seen that the negative ionic gas provided to the dried yeast by the negative ion treatment acts to promote cell growth of the cultured yeast.

That is, in the case of the discharge treatment in Experiment A, the negative ionic gas in the discharge atmosphere promotes the cell growth of cultured yeast, but the minute amount of positive ionic gas contained in the discharge atmosphere and the oxidation of NOX, etc. It is presumed that the substance acted as an inhibiting factor, resulting in a different result from Experiment B1. As can be seen from Figure 7, as far as this experiment is concerned, the effect of 24-hour treatment is not large, but when treated for 7 hours and 7 hours, the growth rate of yeast cells is lower than that of untreated cells. Significantly improved.

FIG. 8 shows the relationship between the body time of cells and the negative ion treatment time.

Here, the cell critical values are shown in Table 2, and the rate of change is within a relative error of 2.4%.

Figure 9 shows the number of days required to reach the critical value, and while it took approximately 9.5 days without treatment, the number of days required to reach the critical value was reduced by 5.5 to 6.0 days with negative ion treatment. It turns out that it can be shortened to a day. Next, FIG. 10 shows the results of experiment B2, that is, the case where the yeast being cultured was cultured under continuous negative ion treatment by providing an atmosphere containing a negative ionic gas.

As in Experiment B1, when culturing is performed after negative ion treatment, the effect appears at the same time as the start of culture, whereas in the case of this experiment, the effect appears with a time delay. In other words, just as in Experiment B1, cell proliferation accelerated when negative ions were treated for a certain period of time, in this experiment as well, the effects of continuous negative ion treatment appeared only after negative ion treatment for a certain period of time. There it is. In this case, cell proliferation will become active after the 3rd day of culture, ie after 7 days of continuous treatment. In addition, in this experiment B1, dried yeast was treated in a negative ion gas atmosphere in a flask, whereas in this experiment, it was treated through a culture solution in a negative ion atmosphere, so there was no effect of negative ions. It is presumed that as a result of being reduced by the culture solution, the delay was delayed until n hours had elapsed as described above.

Also, when yeast in the culture medium is continuously treated with negative ions in this way, negative oxygen ions are dissolved in the culture medium, increasing the concentration of active oxygen in the culture medium, and inhibiting the respiration of yeast during culture. It is presumed that it is absorbed by the cells, activates its metabolic effects, and promotes cell growth.

In the end, from the experimental results of the above-mentioned discharge treatment, negative ion treatment, and negative ion continuous treatment, the following can be found.

That is, by creating a negative ionic gas by discharging it in the atmosphere using a discharge generator, and treating the dried yeast in an atmosphere containing this ionic gas for a predetermined period of time,
It promotes changes in the biological properties of yeast, promotes the growth of yeast during culture, and significantly shortens the generation time while maintaining the critical cell number, dramatically increasing the rate of yeast division and proliferation. I can do it.

Generally, when a discharge is made in the atmosphere, various gas molecules (02, N2, H2O, etc.) in the atmosphere are excited by the discharge, and positive ionic gas is generated, and the electrons released by the discharge are Due to the action of the leakage electric field of the neutral particles of molecules, various negative ionic gases are generated by adhering to the neutral particles.

In the case of weak current discharge, the excitation energy is insufficient, so there is very little positive ionic gas, and most of the ionic gas is occupied by negative ionic gas.

It is quite possible that these negative ionic gases have various effects on the biological activities of yeast.

In other words, negative ionic oxygen is active oxygen and has the effect of greatly promoting the metabolic effects associated with respiration during pure culture of yeast, thus promoting the growth of yeast and accelerating its proliferation rate. Probably.

As already mentioned, the effect of treating not only cultured yeast but also dried yeast will be felt on the cultured yeast, which cannot be explained solely by the oxidation effect of metabolism. It is difficult. Microorganisms such as yeast produce various enzymes inside and outside of their bodies, and maintain their biological functions by performing complex chemical reactions with enzymes, which are trace amounts of chemical substances. It is also possible that the ionic gas has a direct or indirect effect on the enzyme.

That is, it can be considered that the enzyme possessed by the dry yeast was affected by the ionic gas, and this enzyme affected the yeast during culture.

Next, an experiment conducted to investigate the influence of ionic gas caused by discharge on chlorella and alumia will be described.

Chlorella belongs to the unicellular algae among microorganisms, and absorbs solar energy with high efficiency, performs active photosynthesis, and uses water and carbon dioxide as raw materials to produce white matter containing large amounts of essential amino acids and various vitamins. Therefore, it is considered a promising alternative food and feed source to solve future food shortages.

The following experiment was conducted to examine whether changes in biological properties of Chlorella and Artesia could be promoted by treatment in an atmosphere containing ionic gas caused by electrical discharge. -Experiment 1 on Chlorella (1) Experiment method When a silent discharge generator is used as the discharge generator (Experiment C) The silent discharge generator E'' shown in Fig. 11 is used to discharge in the atmosphere, and ionic gas is generated. A containing atmosphere was generated, and this atmosphere was supplied to the chlorella under cultivation using air pump A, as shown in FIG.

As this supply method, as shown in FIG. 12, experiments were conducted on three types of treatments: aeration method treatment W, irradiation method treatment G, and no treatment N.

The discharge generating device E'' shown in FIG. 11 has a lead disk anode 7 and a needle-shaped cathode 8 placed opposite each other in a glass tube 6 and applies a DC high voltage to generate a discharge. A discharge is caused in the atmosphere supplied from the glass tube 10, and this discharge atmosphere is taken out from the other glass tube 10.

In addition, the supply of discharge atmosphere (hereinafter referred to as discharge treatment)
2-8 after the start of culture. After the first treatment over a period of time, the second treatment was performed for 26 to 31 hours after the start of culture, and changes in cell number over time were measured.

(■) Experimental results and results of study experiment C are shown in Figures 13-1.
It is shown in Figure 6.

Figure 13 shows [Time-dependent change in the number of cells of discharge-treated chlorella with a diameter of 2.6 μm or more], Figure 14 shows "comparison of the number of cells in discharge-treated chlorella with a diameter of 2.6 μm or more", and Figure 15 shows "
1.
Figure 6 shows "comparison of total cell numbers of discharge-treated Chlorella".

In addition, the symbols 11 and 12 in the figure are the first processing section and the second processing section, respectively.
The treatment sections are shown, and the symbols 0-O, 4, and ● indicate no treatment, irradiation method treatment, and aeration method treatment, respectively.

Regarding Experiment C - Figure 13 shows changes over time in the number of large Chlorella cells with a size of 2.6 μm or more.

The untreated cells showed a nearly linear increase in cell number, and no significant change was observed in the discharge-treated cells. However, in the first treatment section and the second treatment section, the influence of the discharge treatment was clearly evident. That is, in the first treatment section, cells treated with no treatment and those treated with the irradiation method tend to increase in cell number, whereas those treated with the aeration method show a tendency to decrease. Furthermore, in the secondary treatment section, those treated with no treatment and those treated with the irradiation method showed a decreasing trend, and those treated with the aeration method showed an increasing trend.

As described above, since the number of large cells of 2.6 μm or more is reduced during the discharge treatment, the discharge treatment has the effect of (1) causing some damage to the cells and killing them; It is assumed that there are two effects: (2) an effect of promoting cell division and reducing the number of large cells.

To investigate this, the rate of increase in cell number is shown in Table 3 below. However, it is calculated based on the average number of cells during the treatment period. As can be seen from Table 3 above, in the first period, those treated with discharge showed a higher rate of increase compared to those without treatment, and in the latter period, the tendency was opposite to that of the first period. Therefore, it is clear that the first treatment has the effect of promoting cell division, and the second treatment has the effect of inhibiting cell division and growth.

FIG. 14 shows the change in cell number over time in proportion to that of untreated cells as 100%.

As can be seen from this figure, the aeration method impedes cell division and growth, while the irradiation method causes some impediments, but the 6.5-hour treatment (first treatment) It is judged to have the effect of promoting growth. The above is an examination of large cells of 2.6 μm or more, but in order to investigate the cell division promoting effect of discharge treatment, next we will examine changes over time in the total number of cells.

Figure 15 shows the change in the total cell number over time. In both the first and second treatment sections, the number of cells treated with the aeration method increased significantly, and the number of cells treated with the irradiation method was significantly different from that without treatment. do not have. This indicates that aeration treatment has the effect of significantly promoting cell division. When we investigated the number of cells from the first to second treatments and after the second treatment, we found that the rate of increase in cells treated with the aeration method was extremely small.

Therefore, it is considered that when the aeration method is used, cell division hardly occurs after the treatment. FIG. 16 shows the change over time in the number of cells as a percentage of the untreated cells as 100%.

In the end, the above can be summarized as follows. (1) Although the effects of the aeration method treatment and the irradiation method treatment are not different, the influence appears more strongly in the case of the aeration method treatment.

(2) The influence of the discharge treatment is more pronounced in the influence on the number of all cells including small cells than on the number of cells with a diameter of 2.6 μm or more. (3) Electric discharge treatment greatly affects cell division, and electric discharge treatment for about 6.5 hours significantly promotes cell division. - Experiment on Artemia (Experiment D) - Artemia belongs to the protozoa of higher microorganisms, and has the unique property of re-storing dried eggs by placing them in salt water.

The following experiment was conducted to examine the effect of electric discharge treatment on dried eggs of Artemia on their growth and on their growth.

(1) Experimental method Artemissall from California was used as a sample.
Na dried eggs were placed in a sealed shear dish, into which the ionic gas generated by the above-mentioned discharge generator was supplied for various times (hereinafter referred to as discharge treatment).

The dried eggs subjected to electrical discharge treatment were placed in saline solution to cure the eggs, and changes in the body length of Artemia over time were measured. Two types of experiments were conducted with respect to the treatment time: (1) 2 hours and (2) 3 to 1 hour, but since almost no effect was observed regarding (1), the explanation will be omitted.

Figure 17 shows "changes in average body length of Artemia over time", where symbols 0-O, 1, and ● indicate no treatment, 3-hour treatment, 6-hour treatment, and 12fI!, respectively. Indicates intermediate processing.

As can be seen from this figure, the untreated specimens show a tendency to slow down in growth as early as the 4th day, whereas the discharge-treated specimens show linear growth reaching a peak on the 12th day, and the treatment time The longer the period, the faster the growth. Therefore, it is clear that the discharge treatment has the effect of promoting the growth of Artemia after it becomes stagnant. In order to examine the effect of promoting this growth, we investigated changes in maximum body length over time.

(See FIG. 18) As can be seen from FIG. 18, the same tendency was observed for the maximum body length as for the average body length, confirming the growth promoting effect of the discharge treatment.

Note that the symbols in the figure are the same as in FIG. 17. that's all,
As a result of studies based on various experimental data regarding the effect of negative ionic gas caused by electrical discharge on promoting biological property changes in yeast, chlorea, and Artemia, we found that by providing negative ionic gas to microorganisms for a predetermined period of time, , it was demonstrated that it can promote changes in the bioproperties of microorganisms.

Microorganisms are biochemical entities that produce a variety of enzymes inside and outside of their bodies, and maintain their biological functions through the action of these enzymes.They respond sensitively to changes in the outside world and undergo congenital and acquired changes. Because of this, it is more susceptible to changes in its biological properties due to extremely high concentrations of negative ionic gases compared to natural conditions.

It can be easily inferred from the above experimental results that negative ionic gases also act in various ways on other organisms other than yeast, chlorella, and Artemia.

The present invention is a method for promoting changes in biological properties of microorganisms by generating negative ionic gas using various discharge generating means and treating microorganisms in an atmosphere containing this ionic gas. Unlike physical or chemical methods, a simple, inexpensive, and easy-to-handle device called a discharge generator is used to appropriately select various discharge atmospheres, appropriately select discharge generation means, and adjust processing time as appropriate. By simply setting conditions, it is possible to set various biological property change promoting conditions for various types of microorganisms and promote biological property changes.

Therefore, the present invention is an effective means for changing the biological properties of microorganisms in the applied microbial industry, which is becoming increasingly important in connection with resource problems and food crises. In particular, when the present invention is applied to yeast, which will be required in large quantities for the production of alcohol as an alternative fuel in the future, the growth of yeast can be significantly accelerated and reaching a critical state can be greatly shortened. It becomes possible to culture with high efficiency. Furthermore, when the present invention is applied to Chlorella, depending on the treatment conditions, cell division of cultured Chlorella can be accelerated.

Furthermore, when the present invention is applied to dried Artemia eggs, the growth of Artemia ants after their appearance can be significantly promoted.
Brief explanatory diagrams of the drawings show the apparatus used in the example of the implementation procedure of the present invention and a line diagram of the experimental results. Fig. 1 is a perspective view of the experimental apparatus in which dry yeast was subjected to electrical discharge treatment for experiment A, and Fig. 2 is a diagram showing the experimental results. A
Figure 3 is a circuit diagram of the negative ion generator, Figures 4 to 6 relate to the results of Experiment A, and Figure 4 is a graph showing the change in cell number over time due to discharge treatment. ”, Figure 5 is a diagram showing “discharge treatment time and cell generation time”, Figure 6 is a diagram showing “discharge treatment time and number of days to reach critical value”, and Figure 7 is a diagram showing “discharge treatment time and cell generation time”. ~Figure 10 shows experiments B1 and B2.
Regarding the results, Figure 7 is a line diagram showing ``changes in cell number over time due to negative ion treatment'', Figure 8 is a line diagram showing ``negative ion treatment time and cell generation time'', and Figure 9 is a line diagram showing ``changes in cell number over time due to negative ion treatment''. Figure 10 is a diagram showing the ion processing time and the number of days to reach the critical value.
Figure 11 is a longitudinal side view of the silent discharge generator used in Experiment C. Figure 12 is an explanatory diagram of the discharge treatment in Experiment C. 13
Figures 1 to 16 relate to the results of Experiment C, and Figure 13 shows "2.
Figure 14 is a line diagram showing ``Cell count over time of discharge-treated chlorella with a diameter of 6 μm or more'', Figure 14 is a line diagram showing ``comparison of cell numbers of discharge-treated chlorella with a diameter of 2.6 μm or more'', and Figure 15 is a line diagram showing ``changes over time in the number of cells of discharge-treated chlorella with a diameter of 2.6 μm or more''. Figure 16 is a line diagram showing the ``change in total cell number over time,'' Figure 16 is a line diagram showing ``comparison of total cell number in discharge-treated Chlorella,'' and Figure 17 is a line diagram showing ``change in average body length of Artemia over time.'' Figure 18 is a diagram showing "time-dependent changes in the maximum body length of Artemia."

Claims (1)

[Scope of Claims] 1. A method for promoting changes in biological properties of microorganisms, which comprises treating microorganisms in an atmosphere containing a negative ionic gas. 2. In the method for promoting biotransformation of microorganisms as set forth in claim 1, using dried yeast as the microorganism,
Something that promotes changes in its biological properties and changes its growth rate. 3. The method for promoting biological changes in microorganisms as set forth in claim 1, in which yeast in culture is used as the microorganisms, and changes in biological properties are promoted to change its growth rate. 4. The method for promoting biotransformation of microorganisms as set forth in claim 1, in which Chlorella in culture is used as the microorganism, and its biological properties are changed to promote cell division of Chlorella. 5. In the method for promoting biological changes in microorganisms as set forth in claim 1, dried eggs of Artemia are used as microorganisms, and the biological properties of the eggs are changed to promote the growth of Artemia eggs after they float. .