JPS6034914A - Food containing extract of ganodema licidum - Google Patents

Abstract

PURPOSE:The titled food capable of reducing blood viscosity causing symptom such as suppressing effect on metabolite of arachidonic acid, or hyperlipemia, etc., comprising an extract obtained by extracting Ganodema licidum with hot water to give an extract, extracting further it with an oxygen-containing organic solvent, as an essential component. CONSTITUTION:Ganodema licidum is dried, cut in a sliced or minced state, extracted with warm water, the extracted solution is filtered, to give an extract with hot water. The dried powder of the extract with hot water is extracted with an oxygen-containing organic solvent (e.g., ketone solvent such as acetone, ether solvent such as ethyl ether, etc., alcoholic solvent such as methyl alcohol, etc.), the extractive operations are repeated twice, and a solvent-soluble component is filtered and separated. The filtrate is concentrated, evaporated to dryness to give an extract of Ganodema licidum. A food containing it as an essential compound is prepared to improve hypertension, hyperlipemia, hepatitis, diabetes, unidentified complaint syndrome, etc. This food is in the form of powder or refreshing drink, etc. wherein one can take 3,000-9,000mg of it.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to Reishi mushroom extract Q, and its first objective is to prevent inflammation, blood clots, and preventive measures for those who consume this food by including Reishi mushroom extract in food. Our goal is to provide foods that can suppress arachidonic acid metabolites, which are causative agents of many diseases such as heart disease, arteriosclerosis, and allergies. .

Ganodenna licidum is the J.
L/ / :+ A type of mushroom belonging to the genus O''-, this reishi mushroom has traditionally been widely used as a folk medicine, especially for improving liver function and in recent years, its hot water extraction has been used. This mushroom is known for its anti-cancer properties due to its hemicellrone compound.

The inventors continued intensive research for many years on this Reishi mushroom extract Q, and found that extract 1 based on a specific extraction method of Reishi mushroom suppresses the metabolism of arachidonic acid in serum.
Furthermore, the inventors have discovered that symptoms caused by high blood viscosity, such as hypertension, hyperlipidemia, hepatitis, diabetes, and indeterminate complaint syndrome, can be improved by lowering blood viscosity, leading to the present invention.

This invention is based on Ganoderma lic.
The invention relates to a food containing a Reishi mushroom extract whose essential ingredient is an extract obtained by further extracting a hot water extract of M. idum with an oxygen-containing organic solvent.

The Reishi mushroom extract used in this invention can be obtained by, for example, drying Reishi mushrooms, cutting the dried Reishi mushrooms into slices or minced pieces, and soaking the cut Reishi mushrooms in hot water at about 60°C to 70°C. Shiba 100! Add about 11 parts of water to I + 1
Extraction is carried out for about 2 hours, and the extract is distributed from person to person to obtain a hot water extract.

Furthermore, dry powder of this hot water extract (about 50.7%) was extracted with acetone (11) using acetone as the oxygen-containing organic solvent, and this extraction operation was repeated twice to remove the acetone soluble content. The solution F may be concentrated to dryness or made into an aqueous solution with only acetone volatilized.

In this invention Q, especially when the Ganoderma extract is extracted with hot water and further extracted with a dioxygenated organic solvent,
Compared to cases where conventionally known Ganoderma extracts are extracts that have only been subjected to hot water extraction treatment or are water-soluble extracts of the oxygen-containing organic solvent insoluble portions of this hot water extract, It has been clearly demonstrated through the experimental knowledge of the inventors that this effective effect can be exerted.

Oxygen-containing growth solvents that can be suitably used in this invention include ketone solvents such as acetone and methyl ethyl ketone, and 71% ether solvents such as ethyl ether and methylal.
Alcohol solvents such as methyl alcohol and ethyl alcohol can be suitably used.

The Reishi mushroom extract obtained by such a method is usually used in the form of food according to the present invention. (The eater is 11"3.
000mg to 9,000mg can be taken as a powder, soft drink, or any other food form.

Next, the effects of the present invention will be made clearer by showing examples and test examples according to the present invention.

Example 11 cg of Ganoderma lucidum was prepared, dried, cut into pieces with a thickness of about 5 mm, and then ground into a powder of 60 mm.

Next Reishi 100% in warm water of 70℃. ! Add 11 parts of water to the 7 parts and extract for 2 hours. The extract is separated from the remaining part of the liquid F. Take out the remaining part and grind it into minced pieces. water is added thereto, heated to about 70°C for about 3 hours to perform an extraction operation, and then added to the above-mentioned extract solution.

Note that this F solution was compressed by 5 liters under reduced pressure.

Hot water extract obtained in this way (50g, but powdered)
was added to the acetone soluble matter and extracted for 1 hour.Then the mixture was taken from house to house and 11 acetones were further added to the residue and extracted with stirring under the same conditions.

This stirred mixture was also sent door to door, and the P solution of both acetone extracts was collected, and the F solution was dried under reduced pressure to obtain 784 g of acetone extract.

Comparative Example The acetone-insoluble matter collected from each household was collected, and to the acetone-insoluble matter Q, 400 g of water was added at room temperature, and the water was stirred and mixed to obtain a water-soluble m portion.

In this state, the P solution was evaporated to dryness to obtain a water-soluble component of 37.7.

Test Example 1 Wistar King Oslano 1- (250-350g)
This rat blood was collected using

5. EDTA was added as an anticoagulant to this rat blood.
8 mM was added and the blood was then centrifuged at 2201 for 10 minutes at room temperature to remove platelet-rich plasma).
Centrifugation was performed for 10 minutes at 1, 5 oox, and y. The platelets obtained in this state were added to a HEPES-physiological saline buffer containing 2mM EGTA (25mM HEPES, 35mM Na
Wash twice with C1, PH7, 4), and wash the washed platelet suspension 1! )is.

71J with this washed platelet suspension and Examples and Comparative Examples]
O~1.000)'g/m! 3 was added for pre-incubation at 37°C for 5 minutes. Next QC1-"C) Arachidonic acid (0.05 μCi, manufactured by NEN) was added and incubated for 5 minutes. After this, phosphoric acid was added to stop the reaction, and arachidonic acid metabolites were extracted with ethyl acetate. This extract was dried in a nitrogen stream, and a small amount of ethyl acetate was added to the dried product, which was quantitatively spotted on a silica gel plastic sheet (No. 5748 manufactured by MercK) and developed.

The developing solvent was taloloporum: methanol: acetic acid: water (90%
:8 :l :0.8. molten ratio) was used.

Radioactive metabolites were detected using an autoradiograph, silica gel sheets corresponding to the spots were cut out, and radioactivity was measured using a liquid scintillation counter. Three main spots appeared in such a system. 1-
Lombosaquin B2 (TXB2) 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT
) and 12-hydroxy-5,8°10.14-eicosatetraenoic acid (12-1-TETE).

TXB2 and HHT are metabolized from arachidonic acid via chromooxygenase, and l2-HETE
is metabolized via 12-ribogysigenase. TXB2 is a stable conversion product of TXA2 that has strong platelet aggregation and vasoconstriction effects. Note that TXB2 was identified by TLC with a known TXB2 standard product. Also 12-
HETE was identified from GLC-MSG of the trimethylsilyl derivative of its methyl ester.

The results are summarized in Table ζ.

The numerical values in Table 1 indicate the amount of raw acid in TXB, taking the value of no treatment as 100%.

Test Example 1 <Subjects: 36 cases of high viscosity blood group with hypertension, hyperlipidemia, hepatitis + urinary disease, undefined complaint symptoms trr> Among the above diseases, 30 cases of high viscosity group Q'' - 3g of Ganoderma lucidum extract was administered, and 6 cases Q were administered 9g of Ganoderma extract.

Judgment> In the former case, 3g of Ganoderma extract was administered, many patients showed improvement in symptoms, but by 4 months, Jfn
There was no significant improvement in liquid viscosity.

In the latter group administered 9 g of Ganoderma extract, blood viscosity was significantly reduced;
Improvements in α-riboprotein were observed. Symptom improvement was also noticeable.

Test Example 2 <Case> (]) 56 O ♀ IJi: 6 Hemiplegia after thrombosis 4 years ago Q. He had a history of cerebral thrombosis, and currently visited the hospital complaining of a feeling of tingling in his right hand. Moxibustion and reishi extract on his right hand. Thing 9I74
The condition improved after 4 months.

Improvements in blood viscosity and β-ribo were observed.

Cases > (2) 58 years old ♀ Hypertension, menopause iJ I
There was a history of hypertension prior to this report. She also has other menopausal symptoms and suffers from the symptoms of ``fullness of ears''.

Reishi extract9. ! After taking 7/ro, his blood pressure stabilized after one month, and his symptoms of ``fullness of ears'' improved after two months.

(3) Hypertension Feeling of numbness on the right side of the body. A CT scan revealed no abnormalities.

Reishi extract 1'/B formulation. After four strokes, his blood pressure stabilized, and his hand/fin rash improved.

There was an improvement in β-lipo.

Case example (4) Menopausal disorder I have stiff shoulders, lower back pain, and hot flashes.

I hope to improve my physical condition.

Prescription of Reishi extract 9g/day. Within 1 month, an improvement in blood viscosity and β-ribo and symptoms were observed.

<Case> (5) Chronic hepatitis Diagnosis of chronic hepatitis 5 years ago. Since then, at least GOT, G
I have never had a PT of less than 50G.

Reishi extract 9! /When I was prescribed IE, GOT and GPT became less than 50 after 7 months and remained unchanged for 6 months.

Case example (6) Chronic hepatitis Diagnosed with hepatitis two years ago.

Two months after taking ganoderma extract 91 days a day, the test values improved.

Blood viscosity and β-lipo levels improved.

As is clear from the above results, the specific Reishi mushroom extract used in the food according to the invention Q has an excellent effect on suppressing the metabolism of arachidonic acid and improving hyperviscosity blood disease.

Claims (1)

[Claims] 0) Ganoderma 1 icido+
A food containing a Ganoderma lucidum extract whose essential ingredient is an extract obtained by extracting the hot water extract of n) with a further oxygen-containing organic solvent.