JPS603472B2 - sensitive disc distributor - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a disk distributor for accurately positioning sensitive disks (hereinafter simply referred to as disks) on the surface of a culture medium.

One of the widely used methods for testing antibacterial properties against pathogenic bacteria is the disk method, which involves impregnating a special circular (8 ribs or 6 sides) oven paper with a certain amount of antibiotics. A disc that has been pressed is used.

An example of a testing method using this disk is to uniformly smear microorganisms isolated from a specimen (pus, lacquer, blood, urine, etc.) collected from a patient onto the agar medium surface of a petri dish, and then Disc on the surface (e.g. 2 units of penicillin/disc)
Touch it again, press it lightly, and cover the dish with a lid.
Cultivate in an incubator. After culturing, bacteria grow all over the surface of the medium, but a circular growth inhibition circle is observed in a certain area around the disk where the bacteria do not grow because penicillin has diffused from the disk. . The operator carefully observes this inhibition circle, determines the diameter of the inhibition circle, and calculates the amount of drug to be administered to the patient. In the above-mentioned disk method, many specimens are brought into the actual laboratory, and it is common to calibrate the disks of different drugs for one specimen. Furthermore, there is a need for a disc distributor that can dispense the seeds horizontally and in close contact with the culture medium surface.

Recently, Tsubaki Sekisho No. 52-64755, No. 55-3976
3 have been proposed, but all of them have complicated mechanisms to provide the above-mentioned functions. The object of the present invention is not only to satisfy the various performances that a disk distributor should have, but also to simplify the mechanism of the disc distributor.

To achieve this purpose, a cartridge containing a large number of disks is removably supported in the horizontal direction on the side of a base supported by legs that straddle the bacterial culture dish, and the tip of the cartridge is facing the cross-linked portion. A chute having a disc ejection path is provided on the lower side so as to be elastically slidable, and a pressure piece is provided at the lower end of the chute, which flattens the disc that has fallen flat on the disc receiving plate. Press lightly.

Further, above the base, a spindle rod is connected to the upper guide tube, and has an ejecting piece for ejecting the discs in the cartridge one by one onto the shooter and the Oka line, and a pressing piece for sliding the shooter. One of the disks in the cartridge is ejected from the cartridge by an ejection piece when the spindle rod is pressed down, and it falls vertically within the ejection path of the chute and onto the receiving plate. At the same time, the push-down piece lowers the shooter so that it comes into flat contact with the disk on the receiving plate by the pressure piece at the lower end, and as the shooter descends, its tip touches the receiving plate. The disc is characterized in that the cut is expanded and the disk is dropped flat on the surface of the bacteria culture surface, and the disc is brought into close contact with the surface of the bacteria culture surface by the weight of the pressing piece.Next, an embodiment of the present invention shown in the drawings This will be explained in more detail.The legs 1 are placed so as to straddle the petri dish B containing the bacterial culture medium A.
The base 2, which is supported by the handles, is fixed.

The base 2 is a rectangular block made of resin such as polyvinyl chloride, hard polystyrene, hard polyethylene, acrylic, polypropylene, polycarbonate, etc. or metal such as aluminum. Preferably, the material is made of aluminum, duralumin, or the like. A socket hole 3 is formed in the horizontal direction approximately at the center of the side surface of the base 2, and a cartridge C can be detachably inserted into the socket hole 3.

As is particularly clear in FIG. 3, the cartridge C has an opposing protrusion C protruding in the diametrical direction at one end, and a guide groove 4 (a second
(see figure), the protrusion C is engaged with and inserted.

Also, a bridge portion C2 is provided at the tip of the cartridge C so as to be slightly larger than the thickness of one disk D from the tip of the cartridge C.
The spring C3
The disk ○ is pressed against the bridge portion C2. Then, the cartridge C is inserted into the socket hole 3 so that the entrance between the bridge portion C2 and the tip of the cartridge C is perpendicular and stops at a predetermined position. There is. An upper guide tube 6 and a lower guide tube 7 are respectively fixed to substantially the center of the upper and lower surfaces of the base 2, facing the bridge portion C2 and located on the same vertical axis with the bridge portion C2 as the center. There is.

A cylindrical tube 9 connected to a knob 8 is inserted into the upper guide tube 6 and is elastically movable with respect to the upper guide tube 6 by a spring 10, and a spindle rod that moves inside the tube 6. 11 is loosely engaged with the knob 8 at its upper end, and at its lower end is attached a discharge piece 12 that vertically passes through the closing portion of the bridge portion C2 of the cartridge C, and a push-down piece 13 arranged in parallel therewith.

Further, the spindle rod 11 is provided with a flat head screw 15, which engages with a linear guide groove 6' formed in the upper guide tube 6. Further, the pin 14 provided in the upper guide tube 6 is formed in the cylindrical tube 9 and is connected to the joint tube 9.
In the descending movement of the two-stage descending interlocking mechanism B, for example, the step
6'. In addition, 17 is a stopper, which adjusts and fixes the upper and lower sides of the stopper 17 with a fixing nut 18, and engages with the lower end of the gutter pipe 9.
Determine the lowering limit of A key groove 7' is formed inside the lower guide tube 7 of the base 2, and the shooter 19, which has a key part 19' that engages with the key groove 7' and moves up and down, is elastically supported by a spring 20. The shooter 19 is formed with a discharge path 21 having a rectangular cross-section so that the disc ○ falls vertically along its plane, and a discharge path 21 having an approximately half-moon shape in plan at its lower end. The pressurizing piece 22 has an interval equal to the discharge path 21, and the small pin 24 and the long groove 23' of the outer wall of the housing joint part 23 are engaged in the housing cylinder part 23 at the lower end of the shoe/tube 19. , pressure piece 22
Due to its own weight, it normally hangs downward.

Furthermore, the shooter 1 is attached to the lower end of the lower guide tube 7.
A cannula 25 having an inner diameter through which the cannula 9 can be inserted is attached, and a tube thread 26 is further screwed to this cannula 25, and a receiving plate 27 is held by being held between this tube screw 26 and the cannula 25. has been done. This receiving plate 27 is made of a thin plate with good flexibility, such as a synthetic resin or metal plate, and has a large number of cuts 27' formed radially from the center, as shown in FIG. 4, for example. Next, the operation of the sensitive disk distributor of the present invention constructed as described above will be explained.

Set the distributor over the petri dish B containing the bacterial culture medium A, align the guide groove 4 in the socket hole 3 of the base 2 with the protrusion C of the cartridge C, and insert the cartridge C into the socket hole 3. Insert until it stops inside.

Then, the bridge portion C2 is located at a predetermined position, that is, the outer periphery of the most advanced disk D in contact with the bridge portion C2 and the tip position of the discharge piece 12 are located on the same vertical line. Then, push down the knob 8.

Then, the cylindrical pipe 9 and the spindle rod 11 descend straightly while being guided by the pin 14 and the bent groove 16, as well as the flat face screw 15 and the guide groove 6'. Therefore, the pin 14 is bent into the bent groove 16.
When it comes into contact with the stepped portion 16', the first step of the downward movement stops once, and along with this downward movement, the discharge piece 1
2 comes into contact with the disk D and presses it to form the bridge portion C2.
A discharge path 2 protruding further and provided in the shooter 19
1, the disk D, changes its posture to a horizontal plane on the receiving plate 27, and when the disk D, stops flat and stable (as shown by the chain line in Fig. 2), the disk D. At the same time, the tip of the pressing piece 13 engages with the top surface of the shooter 19. In order to ensure this engagement, it is preferable to form a recess in the top surface. The knob 8, which has first stopped at the stepped portion 16', is rotated a little along the stepped portion 16' at the bent portion of the bent groove 16. Since the knob 8 and the spindle rod 11 are in an idle state, this rotation is caused by the knob 8 and the cylindrical tube 9.
is rotated. When this rotation is completed, the pin 14 is located again in the straight part of the bent groove 16, so the second stage of downward movement begins, and the push-down of the tab 8 becomes possible. The lower surface of the pressure piece 22 comes into contact with the disc D on the receiving plate 27, and the shooter 19 is further lowered while being held down by the weight of the pressure piece 22 so that the disc D on the receiving plate 27 does not shift. The lower end is the receiving plate 27
(Meanwhile, the pressure piece 22 is in contact with the small pin 24 and the long groove 23'
) and the cut 27' of the receiving plate 27.
When the disk D is pushed open and finally opened beyond the size of the disk D, the disk D comes into contact with the surface of the bacterial culture medium A while receiving the weight of the pressure piece 22, and is appropriately pressurized by the weight of the pressure piece 22. and good disk distribution is achieved. (See Figure 6)
After dispensing, the return movement of the spring 10 causes the pin 14 of the upper spindle rod 11 to return to its original position along the gentle curved portion 16'' of the bent groove 16, and the shooter 19 is moved upward by the pressing force of the push-down piece 13. When the discharge piece 12 returns to its original position through the spring 201 and comes out of the bridge portion C2, the disk D is sent out by the spring C3 so as to come into contact with the bridge portion C2.

In addition, when distributing various types of disks to a large bacterial culture medium at the same time, it is sufficient to arrange a large number of bases 2 of the present invention in parallel. For example, as shown in FIGS. A plurality of bases 2a, 2'a, 2b, . . . (in this example, 4
The first bases 2a, 2'a, 20, .
Lower guide tubes 7a, 7'a, 7b..., sleeve tubes 25a, 25'a, 25b..., pipe screws 26a, 26', which have built-in shooters, pressure pieces, receiving plates, etc., as in the embodiment shown in the figures.
a, 26b..., and fix the bases 2a, 2'a, 2b...
...on the top surface, almost the same as in the case of Fig. 1 above,
Upper guide tubes 6a, 6'a, 6b, respectively.
is fixed, and inside this is a spindle rod 1 that slides up and down without rotating due to the guide groove and flat head screw.
1a, 11'a, lib... and the press-down piece 13 attached to this.
a, 13'a, 13b... and discharge pieces 12a, 12'a, 1
2b... are respectively provided, and a vertically moving plate 28 is iron-coupled to the upper guide tubes 6a, 6'a, 6b, . . . so as to be vertically movable. Each of the rods 11a, 11'a, 11b... is connected.

A stud 29 is provided approximately at the center of each base 2a, passing through the vertically moving plate 28, and a spring 10 is interposed between the vertically moving plate 28 and the base, and the stud 29 is connected to the vertically moving plate 28. The pin 14 is iron-coupled to the cylindrical tube 9 having the /b 8 fixed thereto, and the pin 14 fixed to the stud 29 engages with the bending groove 16 of the two-stage downward movement mechanism E and is guided.

C is a cartridge, each base 2a, 2'a, 2
b... are inserted into each of them. The operation is the same as in the case of use described above, except that all the spindle rods are simultaneously operated by the vertical movement plate 28, and the two-stage downward movement is performed by pressing down the knob 8.

Furthermore, since the rotating movement of the tube 9 at the bending part in the two-stage downward movement by the pin 14 and the bending groove 16 described above is not necessarily necessary, the descent of the tube 9 is temporarily stopped. For example, a mechanism such as the click 5 of the base 2 described above may use a spring or the like to temporarily stop the descending of the cylindrical tube 9 by applying resistance.

In this way, the present invention has a mechanism in which the disks are mounted in the horizontal socket holes with their planes being vertical, and the disks are ejected one by one upwards from this point, and the disks are ejected downwardly. Since the mechanism for distributing the loaded disks to the culture medium is separated from the mechanism, the unit can be replaced and repaired, and since the ejection path is vertical, the disks fall quickly and reliably after leaving the cartridge. Yes, when distributing the disks, the disks are corrected on the receiving plate in a vertical plane before being fed, so the disks can be distributed in a stable state and in close contact with each other.

[Brief explanation of drawings]

The drawings show an embodiment of the present invention, and FIG. 1 is a vertical sectional front view, FIG. 2 is a partially cutaway side view, FIG. 3 is a perspective view of the cartridge, and FIG. 4 is a perspective view of the receiving plate. , Figure 5 is Figure 1 V
-A cutaway plan view taken along the V line, FIG. 6 is a cutaway front view at the time when disks are distributed and supplied, FIG. 7 is a partially cutaway front view showing an embodiment of the present invention in which a large number of disks are distributed simultaneously, and FIG. 8 is also a side view. Symbols, A and A' are bacterial culture medium, B and B' are petri dishes, C is cartridge, D is disk, E is two-stage descending movement mechanism,
2, 2a... are the base, 3, 3a... are the socket holes, 6, 6
a... is the upper guide tube, 7, 7a... is the lower guide tube, 8 is the knob, 9 is the cylinder tube, 11, 11a...
... is a spindle rod, 12, 12a... is a discharge piece, 1
3, 13a... are pressing pieces, 14 are pins, 16 are bent grooves, 1
9 is a shutter, 12 is a discharge path, 22 is a pressure piece, 27 is a receiving plate, 28 is a vertical movement plate, and 29 is a stud. Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6-7 Figure 8

Claims (1)

[Claims] 1. A horizontal socket hole is provided in a base that can be positioned across the bacterial culture medium, a cartridge containing a disk is stably inserted into the socket hole at a predetermined position, and the tip of the cartridge is cross-linked. An upper guide tube and a lower guide tube are provided on the same axis above and below a base that almost faces the section, and a discharge piece and a pusher are attached to the lower end of a spindle rod that slides inside the upper guide tube and performs a two-step downward movement. A shutter having a discharge passage is slidably provided in the lower guide tube, and a pressurizing piece is suspended from the lower end of the shutter, and an opening larger than the size of the disk is formed below the shooter. The disk ejected from the cartridge by the ejecting piece in the first stage action of the two-stage downward movement falls down the ejection path, changes its attitude in a horizontal plane on the receiving plate, and at the same time The shooter descends in a two-step downward motion, and the pressure piece at the bottom presses the disc on the receiving plate, opening the receiving plate and allowing it to fall onto the bacterial culture ground, and applying a light pressing force to the disc with the pressure piece. Features a sensitive disk distributor. 2. The sensitive disk according to claim 1, wherein the two-step downward movement comprises a pin provided in the upper guide tube and a bending groove having a step provided in the cylindrical tube surrounding the guide tube. Distributor. 3. A sensitive disk distributor according to claim 1, wherein the discharge path in the shooter has a rectangular cross-sectional shape so that the disk falls perpendicular to the plane. 4. The distributor according to claim 1, wherein the receiving plate is a thin plate with a smooth surface and high flexibility, and has a large number of radial cuts starting from the center thereof.