JPS60258126A - Production of polymerized hemoglobin - Google Patents

Production of polymerized hemoglobin

Info

Publication number
JPS60258126A
JPS60258126A JP11352584A JP11352584A JPS60258126A JP S60258126 A JPS60258126 A JP S60258126A JP 11352584 A JP11352584 A JP 11352584A JP 11352584 A JP11352584 A JP 11352584A JP S60258126 A JPS60258126 A JP S60258126A
Authority
JP
Grant status
Application
Patent type
Prior art keywords
erythrocytes
blood
hemoglobin
resultant
glutaraldehyde
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP11352584A
Inventor
Teruo Tomota
Katsuro Yagawa
Kiyoo Yajima
Original Assignee
Teruo Tomota
Katsuro Yagawa
Kiyoo Yajima
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Abstract

PURPOSE: To obtain relatively easily the titled hemoglobin capable of being an artificial blood capable of staying in blood for a long period without side effect even by blood transfusion, by adding and polymerizing glutaraldehyde to hemoglobin obtained by subjecting erythrocytes to the hypotonic treatment.
CONSTITUTION: An ACD or CPD preserved blood is centrifuged to separate blood plasma, granulocytes and erythrocytes. 0.9% NaCl solution in a volume of ≥5 times that of the resultant human erthrocytes is added thereto, and further centrifuged to take out the erythrocytes. The human erythrocytes after washing are hemolyzed with a 10mM phosphoric acid buffer solution in a volume of 5 times that of the erythrocytes for hypotonic treatment, and further centrifuged at an ultrahigh speed to separate and remove the erythrocytic membrane component. Glutaraldehyde is then added to the resultant hemoglobin which is a hemolyzed erythrocytic solution, and the resultant mixture is then allowed to stand at room temperature for 1hr. The resultant solution is then passed through a column to separate and remove the liberated glutaraldehyde. Thus, the aimed polymerized hemoglobin having high affinity for oxygen is obtained.
COPYRIGHT: (C)1985,JPO&Japio
JP11352584A 1984-06-02 1984-06-02 Production of polymerized hemoglobin Pending JPS60258126A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP11352584A JPS60258126A (en) 1984-06-02 1984-06-02 Production of polymerized hemoglobin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP11352584A JPS60258126A (en) 1984-06-02 1984-06-02 Production of polymerized hemoglobin

Publications (1)

Publication Number Publication Date
JPS60258126A true true JPS60258126A (en) 1985-12-20

Family

ID=14614548

Family Applications (1)

Application Number Title Priority Date Filing Date
JP11352584A Pending JPS60258126A (en) 1984-06-02 1984-06-02 Production of polymerized hemoglobin

Country Status (1)

Country Link
JP (1) JPS60258126A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63297330A (en) * 1987-05-05 1988-12-05 Chiyan Shia Jien Pasteurizable and freeze driable hemoglobin-base substitute blood
JPH05502240A (en) * 1990-08-17 1993-04-22

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS51109983A (en) * 1975-02-27 1976-09-29 Alza Corp

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS51109983A (en) * 1975-02-27 1976-09-29 Alza Corp

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63297330A (en) * 1987-05-05 1988-12-05 Chiyan Shia Jien Pasteurizable and freeze driable hemoglobin-base substitute blood
JPH0532372B2 (en) * 1987-05-05 1993-05-14 Shia Jennchan
JPH05502240A (en) * 1990-08-17 1993-04-22

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