JPS6023299B2 - electrophoretic membrane - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a tortoise phoresing abdomen used for fleas sill analysis.

Tortoise electrophoresis analysis uses a flea electrophoresis membrane manufactured by coating or casting a membrane-forming material such as agar, cellulose, cellulose acetate, starch, silica gel, or polyacrylic acid gel on a support such as a glass plate or transparent plastic. It is immersed in a buffer solution, the substance to be analyzed is applied on top of this, a DC voltage is applied to it, the substance is electrophoresed in the pus, its components are fractionated, and if necessary, it is stained and solidified. Quantitative analysis of each component of a substance is performed by measuring the optical density of each component.

Details of flea aerophoresis analysis and dragon aerophoresis membranes are described in "Flea aerophoresis experimental method (revised 4th edition)" (King 1968), published by the Military Aerophoresis Research Institute, Bunkodo. For electrophoretic membranes used in tortoise electrophoresis analysis, it is considered important for handling that the pus-like material be in close contact with the support. However, films formed by pus-forming molecules such as agar generally have low physical strength;
In particular, it has the defect of being easily peeled off from the support, making treatments such as washing with water extremely complicated and troublesome. For example, serum protein analysis, which is an example of box air phoresis analysis, and serum protein separation using immunofin air phoresis can analyze protein components of more than 3q fleas, and are therefore useful for the clinical diagnosis of facial lesions. Although it is said to be the most effective method, the time required for washing with physiological saline and distilled water during processing in this immunoelectrophoresis method can exceed 20 hours, accounting for approximately 50% of the total processing time. This is the current situation.

The reason why the water washing process takes such a long time is that the membrane itself has a weak physical strength and easily peels off from the support, so it can only be washed gently under an extremely weak stream of water. If washing could be done under a strong rope or with a strong water stream, it would be easy to shorten this washing time to 1/2 to 1/3, and the total processing time would be 60 to 70 minutes. It was possible to shorten the time by approximately %. In this way, improving the physical strength of the membrane itself, especially its peel strength, has become one of the most important issues for box phoresis membranes, but to do so, it has become difficult to improve the physical strength of the membrane itself, especially the peel strength. It must be absolutely avoided to cause
At present, an effective solution has not necessarily been obtained.

An object of the present invention is to provide an electroperiodic membrane with high physical strength. The inventors of the present invention have conducted extensive research to achieve the above object, and have discovered that the above object can be achieved by incorporating mannan into the film-forming material.

As for mannan, "Chemistry Dictionary 8" (Kyoritsu Shuppan, 196
2 King Publishing), “Iwanami Rii B-Gaku Dictionary” 3rd edition (Iwanami Shoten,
(Published in 1971), “Illustrated Handbook of Carbohydrate Chemistry” (Kyoritsu Shuppan,
Mannans derived from various plants and microorganisms, such as those described in 1971 (published in 1971) can be used.

Mannaso can improve the physical strength of fly aerosol membranes without impairing their performance, whether used together with other pus-forming materials such as agar or cellulose, or alone. Mannan is required to be contained in an amount of 0.5 M% or more in the film forming material.

If it is less than 0.5 wt%, sufficient physical strength cannot be obtained.

There is no particular restriction on the upper limit of the content, but 5wt%
Since there is no particular benefit in terms of the performance of the aerophoresis membrane even if the content exceeds this amount, it is usually sufficient to contain the content in an amount of 0.5 to 5% by weight. When using mannan alone, 0.5 to 5wt
% mannan aqueous solution, 0.1 to t% of basic salts such as sodium carbonate, potassium carbonate, calcium carbonate, etc. are added and further flooded to form a uniform Taisen sap, which is poured into glass. It may be applied or cast onto a support such as a plate or transparent plastic. The same applies when used together with other film-forming materials such as agar and cellulose.

According to the present invention, the physical strength of the fly aerophoretic scale membrane is improved, so it is easy to wash with water during electrophoretic analysis, and it can be carried out without fear of peeling even under a strong lock or under vigorous running water, making it suitable for analysis. Not only can the time required be significantly shortened, but also the handling for storage is extremely easy.

Examples will be given below in order to make the effects of the present invention more clear.

Example 1 part by weight of konjac mannan and 100 parts by weight of water were placed in a beaker and kept at 40 qo/5°C for 2 hours with occasional lighting.
1 part by weight of aqueous salt was added in powdered form and rapidly lit.

After stirring for 2 hours, pour this aqueous solution onto a glass plate with the thickness of 2 kites.
It was cast to the thickness of the skin.

After 1 minute, the film was washed with running water to remove the alkali, but no peeling of the film occurred.

Thereafter, it was immersed in a buffer solution and stored. Three days later, serum proteins were analyzed by immunoelectrophoresis using this turtle phoresis membrane.

Claims (1)

[Claims] 1. An electrophoretic membrane characterized by containing mannan as a membrane-forming material.