JPS6020017B2 - Syringe type separation tube - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION This invention relates to a syringe-type separation tube in which a part of the syringe body is provided with an opening/closing membrane so that collected liquid can be separated.

Conventionally, serum for blood tests is collected by putting the collected blood into a centrifugal test tube, separating the serum and blood, and then collecting the upper layer of serum with a syringe, but when handling a large number of samples, Collecting with a syringe was time-consuming, and the syringe used had to be replaced each time, making it difficult to dispose of.

It is also possible to transfer only the serum to the reservoir by gently tilting the test tube in which the serum and the container have been separated, without using a syringe, but this takes time and may contain a large amount of blood. There were drawbacks that could not be addressed in the processing of specimens. Therefore, as a result of research into improving the instruments used for blood serum collection, the present inventors were able to develop a good instrument and achieved the present invention.

That is, an object of the present invention is to solve the above-mentioned conventional drawbacks in serum fractionation, and to provide a method that can perform blood collection, serum separation, and fractionation of serum using an instrument that is convenient to carry out.
The purpose of the present invention is to provide a syringe-type liquid separation tube.

Another object of the present invention is not limited to blood, but when a mixed liquid is separated into a liquid layer and a solvent layer and one of the layers is desired to be obtained, it is possible to perform separation and fractionation from collection of the mixed liquid. The objective is to provide a syringe-type fractional change that can be performed with an instrument.

Next, the present invention will be explained by way of examples with reference to the drawings.

In the figure, 1 is a main body of a syringe divided into a lower tube 2 and an upper tube 3, 5 is an injection needle, and 6 is a piston shank. The lower tube 2 and the upper tube 3 have the same inner diameter and the same wall thickness,
Arranged in series, both tube bodies have removable annular engagement parts 2C and 3A formed at opposite annular ends thereof, and can be freely engaged or removed. When 3A is engaged, the lower tube 2 and the upper tube 3 are held connected.
2A is a thin tube provided at the lower end of the lower tube 2 and serves as a mounting portion for the injection needle 5.

Reference numeral 30 denotes a flange-shaped finger rest provided at the mouth 3C at the upper end of the upper tube 3.

The upper tube 3 and the lower tube 2 are made of synthetic resin or glass, and are transparent or translucent, and in this example are made of transparent acrylic resin. Reference numeral 4 denotes an annular elastic membrane of a predetermined width made of an elastic material such as soft rubber. It is attached to the outer circumferential surface of the joint (joint part).

2B is an annular retaining groove provided on the upper outer periphery of the lower tube 2, 3B is an annular retaining groove provided on the lower outer periphery of the upper tube 3, and both ends of the elastic membrane 4 are provided with retaining grooves. It is inserted into 2B and 3B to prevent it from coming off.

The lower tube 2 and the upper tube 3 are connected by crimping the elastic membrane 4 and are prevented from coming off, so when they are attached to the elastic membrane 4 and connected, the exchange body is pulled apart and the engaging portion 2C
, 3A can be removed, the exchanger can be held at short intervals between both the engaging parts 2C and 3A, and the exchange body can be twisted. The piston rod 6 is made of synthetic resin such as polyethylene, has a flange-like finger hook 6A at its upper end, and has a rubber packing section at its lower end that can be inserted into the connected lower pipe 2 and upper pipe 3. 7 is attached, and the piston bar 6 is inserted into the main body 1 from the packing part 7 side, so that the packing part 7 can slide between the mouth part 3C and the bottom part of the main body 1. Therefore, when using the separation tube 10 of this example, place the iron fitting 5A of the injection needle 5 on the attachment part 2A at the bottom of the main body 1, hold down the finger hook part 6A of the piston rod 6, and hold the packing part 7. is located at the bottom of main body 1.

Thereafter, the piston machine 6 is pulled up in the same manner as a normal syringe, and the blood KE is collected into the main body 1. The amount of blood to be collected is, serum E is required, and when the blood is separated, the amount of serum E layer remaining in the upper part of the lower tube 2, that is, the amount of blood and K is in the lower tube 2 at the bottom. The amount of injection is determined. Next, after the liquid separation tube 10 is erected with the injection needle 5 facing upward, the injection needle 5 is removed, and a rubber cap 8 is attached to the mounting portion 2A.

Next, the cap 8 is turned downward, the separation tube 10 is turned vertically, and the piston rod 6 is removed from the main body 1. Note that it is desirable to adhere a cap (not shown) to the mouth portion 3C of the main body 1. Then, the main body 1 is placed in a centrifuge (not shown) to separate the blood KE in the separating tube 10 into a plate K and blood E. After that, the engaging portions 2C and 3A of the lower tube 2 and the upper tube 3 are separated, a small interval HI is maintained between the engaging portions 2C and 3A of both tube bodies (see Fig. 6), and the exchange bodies are each reversed. After turning and twisting the elastic membrane 4 in the direction to inflate the center part of the elastic membrane 4 so as to cover the opposing ends of both tubes and forming a twisted part 4A (see Fig. 7), both tubes are brought close to each other again and both tubes are closed. The engaging portions 2C and 3A are engaged. Note that since the engagement is performed with the elastic membrane 4 expanded into a thin layer interposed therebetween, the lower tube 2 and the upper tube 3 are held in series by this engagement.

Therefore, there is only serum E in the upper tube 3, which is separated by the elastic body 4 expanded at the engaging parts 2C and 3A of the lower tube 2 and upper tube 3. contains blood, K and some serum E (see Figure 8).

That is, since the serum E in the separation tube 10 is separated by the expanded elastic membrane 4, the serum E in the separation tube 10 is transferred from the mouth 3C of the upper tube 3 to another container (not shown). In addition,
An appropriate inner diameter and length of the separation tube 10 are adopted depending on the blood volume used.

In addition, in the above embodiment, serum was separated from blood, but the liquid to be treated is not limited to this. This device is suitable for separating the upper or lower layer into two layers: organic solvent and organic solvent, and the injection needle attached to the main body can be omitted when collecting the mixed liquid from the liquid tank into the separating tube. , It is possible to pour liquid directly from the pongee pipe at the attachment part of the lower pipe.
In addition, in this example, the engaging parts 2C and 3A are provided at the opposing ends of the lower pipe 2 and the upper pipe 3 so that the exchange can be engaged and held in series. In the case of separating the two layers by using the above method, the engaging means between the lower tube 2 and the upper tube 3 can be omitted.

As explained above, the liquid separation tube of the present invention is a syringe comprising a tube body through which a liquid can flow and has a mounting portion for a syringe needle, and a piston rod inserted into the tube body and sucking and discharging the liquid. The body is divided into an upper part and a lower part, both divided pipes are arranged in series, and an annular elastic membrane of a predetermined width is attached to the side circumferential surface of the opposite end of the exchanger. As a result, the intended objectives of the present invention are certainly achieved.
In other words, since the liquid separation tube of the present invention is of a syringe type, a syringe needle can be attached to the thin tube-shaped attachment part at the bottom of the main body, and the liquid can be detected through the syringe needle or directly from the attached tube without attaching a syringe needle. liquid is possible. In addition, in the present invention, the liquid collected in the main body can be separated into upper and lower layers in the separation tube, and after separation, the upper layer is made of elastic material that expands inside the tube by twisting both tubes by rotating them in opposite directions. It is convenient that the container is divided by a membrane, and the upper layer on the divided membrane can be easily transferred to another container. In particular, the present invention has the advantage that the liquid collection, separation of the two layers of the sampled liquid, and separation of the separated upper layer can be performed using one separation tube, so the number of instruments used is small and the liquid separation operation is easy to perform. It has the following.

[Brief explanation of drawings]

The figures show one embodiment of the present invention, in which Fig. 1 is a parts diagram of a syringe-type separation tube, Fig. 2 is a cross-sectional view taken along the Rolo line in Fig. 1, and Fig. 3 is a diagram of the separation tube. A vertical cross-sectional view, Figure 4 is an explanatory diagram of the state in which blood is collected in the separation tube, Figure 5 is an explanatory diagram of the state in which the cap is attached to the attachment part of the separation tube body, and Figure 6 is an illustration of the separation tube. An enlarged sectional view showing the separation tube when the engaging part is removed, Figure 7 is an explanatory diagram of the action of the separated tubes twisted together, and Figure 8 is the state where they are blocked by an elastic membrane. FIG. 2 is an enlarged explanatory view of the main parts of the liquid separation tube. 1...Main body, 2...Lower tube, 2A...
... Mounting part, 3... Upper tube, 4... Elastic membrane, 4A.
...Twisted part, 6... Piston rod, 7...''/Gookin part, 10... Separation tube. Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8

Claims (1)

[Claims] 1. In a syringe consisting of a tube that can flow a liquid and has a syringe needle attachment part, and a piston rod that sucks and discharges the liquid inserted into the tube, the tube is divided into an upper part and a lower part. The two tubes are arranged in series, and an annular elastic membrane having a predetermined width is attached to the side circumferential surfaces of the opposite ends of the two tubes to cover the ends. liquid tube.