JPS60194330A - Viscosity measuring apparatus - Google Patents

Abstract

PURPOSE:To measure an accurate clotting process, by measuring the inertia moment of a specimen to be measured in such a state that said specimen is sealed in a specific container. CONSTITUTION:The upper and lower ends of an artificial vessel 2, in which blood 1 is injected, are clamped by clamps 3, 3'. A movable coil 4 and a torsion wire 5 are connected to the upper clamp 3 and a torsion wire 5' is connected to the lower clamp 3'. The electrode of the movable coil 4 is connected to amplifiers 8, 10 through a change-over switch 7. The pulse signal generated by a pulse generator 9 is supplied to the coil 4 to rotate the artificial vessel 2 and, when the vessel 2 begins to freely vibrate, the coil 4 generates electromotive force and a blood clotting process is measured from the attenuation ratio and cycle of the wave form of electromotive force.

Description

DETAILED DESCRIPTION OF THE INVENTION (Industrial Application Field) The present invention relates to a viscosity measuring device, particularly for measuring the viscosity or solidification process of a sample held in a specific container while being shielded from the outside air. This invention relates to a viscosity measuring device suitable for.

(Conventional wave/technique) Conventional viscosity measurement devices insert a rod or disk into the sample and measure the viscosity of the sample from the viscous resistance that occurs when the rod or disk is moved. There is. However, depending on the measurement sample, there is a problem in that when it comes into contact with a specific substance, the measurement sample begins to solidify or the solidification rate changes. For example, in order to accurately examine the antithrombotic properties (difficulty of blood coagulation) of an artificial blood vessel, it is necessary to measure the blood while it contains air and does not come into contact with any substance other than the artificial blood vessel, but conventional viscosity measurement Since a part of the measuring device in each device comes into contact with the sample, it has been impossible to accurately examine the antithrombotic properties of artificial blood vessels without conducting animal experiments.

(Object of the Invention) The object of the present invention is to measure the viscosity of a sample sealed in a specific container and to measure the viscosity of the sample inside or the solidification process of the sample inside the specific container. The goal is to provide equipment.

(Structure of the Invention) The viscosity measuring device of the present invention includes a holding means for holding an object to be measured in which a sample is enclosed, and an upper end on which the object to be measured held by the holding means is fixed during receiving. a torsion wire, a part of which is fixed to the holding means and rotates and oscillates the object to be measured, a pair of magnetic poles facing each other with the other moving coil in between, and a pair of magnetic poles that are connected to the moving coil. a switching means having a common terminal and two switching terminals; a signal generator for sending an f pulse signal to the movable coil through one switching terminal of the switching means;
and an instruction signal instructing the generation of a pulse signal to this pulse signal generator, and when a pulse signal of 1 or 4 is generated, the common terminal of the switching means and the one switching terminal are connected to each other. transmitting a pulse signal to the moving coil; in other cases, connecting the common terminal of the switching means and the other switching terminal to output the electromotive force generated from the moving coil from the other switching terminal; The present invention is characterized in that a control means for controlling the pulse signal generator and the switching means is shown in FIG.

The present invention uses the principle that the moment of inertia of a measured object with a sample sealed inside changes depending on whether the inside is in a solid or liquid state. Since the device does not have a member to be inserted into the device or a measuring container of its own, it is possible to measure the viscosity of a blood sample without causing coagulation due to foreign matter, for example.

Therefore, the blood coagulation process for a specific enclosure can be accurately measured.

Although the viscosity measured in the present invention is a relative value, absolute calibration can be easily performed by measuring an object having a known moment of inertia.

(Example) Below, the present invention will be explained in detail with reference to an example. Figure 1 is an example of the viscosity measuring device of the present invention. Figure 1 is a schematic diagram of an example particularly suitable for measuring the blood coagulation process of an artificial blood vessel. be.

An artificial blood vessel 2 into which blood 1 has been injected is clamped at its upper and lower ends by clamps 3.3'. A movable coil 4 is fixedly installed above the clamp 3, and a torsion wire 5 whose upper end is fixed is further connected to the movable coil 4. A torsion wire 5' having a fixed lower end and having the same length as the upper torsion wire is connected to the lower part of the clip 3. Moving coil 4t - magnetic poles 6 facing each other in between
．． 6' is arranged. An electrode of the moving coil 4 is connected to a common terminal 7aK of the changeover switch 7. One switching terminal 7bK of the changeover switch 7 is connected to a pulse generator 9 via an amplifier 8, so that the pulse signal generated by the pulse generator 9 is supplied to the moving coil 4. There is. An amplifier 10 is connected to the other switching terminal 7C of the changeover switch 7 so that the electromotive force generated in the moving coil 4 can be outputted to the outside. - The pulse generator 9 and the changeover switch 7 are controlled by the controller 11, and while the pulse signal is being generated from the Noculus generator 9, the changeover switch 7 is switched in synchronization with the pulse signal, and the pulse signal is changed over. The pulse signal is sent to the movable coil 4, and while the movable coil 4 is freely vibrating, the changeover switch 7 is switched so that the starting force generated by the movable coil 41C due to this free vibration is measured.

The coagulation process of an artificial blood vessel, that is, the measurement of antithrombotic properties using the grooved viscosity measurement device as described above will be explained. As shown in Fig. 1, an artificial blood vessel 2 into which blood 1 has been injected is is held by the clip 3.3' and stretched between the torsion wires 5.5' and 1M. Controller 1
1, the common terminal 7a and the switching terminal 7b of the switching switch 7
In this state, the nof pulse generator 9 is controlled by the controller 11 to generate the nof pulse signal. After this pulse signal is once amplified by the amplifier 8, it is manually applied to the moving coil 4, and the magnetic force generated between the moving coil 4 and the magnetic pole 6.6' causes the moving coil 6 and blood to be injected. After the /4' pulse signal for rotating the artificial blood vessel 2 is sent to the movable coil 4, the common terminal 7a of the changeover switch and the changeover terminal 7C are connected under the control of the controller 11. Artificial blood vessel 2 is a torsion wire 5.5'
It rotates due to the rotational elastic force applied to it and starts free vibration. When the artificial blood vessel 2 starts free vibration, the amplitude and period of this free vibration are observed based on the electromotive force generated in the movable coil 4 which is integrally attached to the artificial blood f2. be able to. Figure 742 shows the output voltage waveform of the amplifier lO when the artificial blood vessel 2 vibrates freely.From this waveform, the amplitude logarithmic attenuation rate Δ=,..., AI and the period 2 are determined, and based on this value, the blood The viscosity can be measured, but in order to investigate the coagulation process, the logarithmic decay rate Δ of the amplitude and the temporal change in the period T are measured. That is, a normal pulse signal is supplied to the moving coil 4 at predetermined time intervals, and in the same way as above, the output voltage waveform 1g of the moving coil 4 based on free vibration (Fig. 3) is converted to the logarithmic damping rate at each time. (Figure 4) and period T (Figure 5). When a substance solidifies, the synchronization T remains constant for a long time, and the logarithmic decay rate becomes small and constant, so by measuring the time until this state is reached, it is possible to The entire blood coagulation process can be investigated.

In the above embodiment, torsion wires were placed above and below the object to be measured, but the lower torsion wire was used to prevent the object from lateral vibration, and the lower torsion wire was connected to a string with no rotational elastic force. Note that the present invention is effective not only for measuring the coagulation process of blood in artificial blood vessels, but also for measuring the viscosity and coagulation process of samples sealed in various predetermined containers. However, for example, it is possible to measure samples that solidify or whose solidification rate changes when they come into contact with air, a probe for viscosity measurement, or the like. Also.

Generates harmful gas and fc! 7" It is possible to measure the viscosity of liquids that react with N gas while they are sealed. Furthermore, it is possible to measure the viscosity of liquids inside ampules and canned goods without breaking the seals. The change in viscosity (solidification time) of a sample solidifying in a specific container can be measured.

If the object to be measured is not a tube-shaped object such as an artificial blood vessel, an appropriate holding means different from the one shown in Figure 1 is used as the holding means. For example, if the container is can-shaped, A device as shown in FIG. 6, in which the object to be measured 2 is held between a pair of discs 31, 31/, and the discs 31, 31' are fixed by a clamp 32, can be used.

(Advantageous Effects of the Invention) As explained in detail, according to the present invention, the coagulation process can be measured without contacting foreign matter with a measurement sample such as blood sealed in a specific container such as an artificial blood vessel. This makes it possible to accurately measure the coagulation process◎

[Brief explanation of drawings]

FIG. 1 is a schematic diagram of an embodiment of the viscosity measuring device of the present invention. Figure 2 shows the voltage waveform obtained from the moving coil when the object to be measured causes free vibration due to the torsion wire, and Figure 3 shows the voltage waveform obtained when a Norse signal is applied to the moving coil at predetermined time intervals. Output voltage waveforms shown in Figures 4 and 5
Each figure shows the period T obtained from the output voltage waveform in Figure 3.
FIG. 6 is a schematic diagram showing another embodiment of the holding means for holding the object to be measured.

Claims (1)

[Claims] a holding means for holding an object to be measured in which a sample is sealed;
A torsion wire whose upper end is fixed for suspending the object to be measured held by the holding means in space, a movable coil whose upper end is fixed to the holding means and rotates and oscillates the object to be measured, and a movable coil that holds the moving coil between the two ends. A switching means having a pair of magnetic poles facing each other, a common terminal connected to the moving coil, and two switching terminals, and a switching terminal connected to the moving coil through one switching terminal of the switching means. An instruction signal for instructing generation of a master pulse signal is sent to the pulse signal generator and the pulse signal generator, and when an I pulse signal is generated, the common terminal of the switching means and said one switching terminal are connected to send a signal to said moving coil, and in other cases, a common terminal of said switching means and said other switching terminal are connected to send a signal to be generated from said moving coil. A viscosity measuring device J0 characterized in that it is provided with a control means for controlling the pulse signal generator and the switching means for outputting an electromotive force from the other switching terminal.