JPS60185163A - 抗トロンビン物質の測定法 - Google Patents
抗トロンビン物質の測定法Info
- Publication number
- JPS60185163A JPS60185163A JP4003284A JP4003284A JPS60185163A JP S60185163 A JPS60185163 A JP S60185163A JP 4003284 A JP4003284 A JP 4003284A JP 4003284 A JP4003284 A JP 4003284A JP S60185163 A JPS60185163 A JP S60185163A
- Authority
- JP
- Japan
- Prior art keywords
- thrombin
- dermatan sulfate
- specimen
- heparin cofactor
- ati
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000126 substance Substances 0.000 title abstract description 10
- 238000005259 measurement Methods 0.000 title description 3
- 230000002785 anti-thrombosis Effects 0.000 title 1
- 239000003146 anticoagulant agent Substances 0.000 title 1
- 108090000190 Thrombin Proteins 0.000 claims abstract description 26
- 229960004072 thrombin Drugs 0.000 claims abstract description 26
- 229920000045 Dermatan sulfate Polymers 0.000 claims abstract description 17
- 229940051593 dermatan sulfate Drugs 0.000 claims abstract description 17
- AVJBPWGFOQAPRH-FWMKGIEWSA-L dermatan sulfate Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@H](OS([O-])(=O)=O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](C([O-])=O)O1 AVJBPWGFOQAPRH-FWMKGIEWSA-L 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims description 13
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims description 8
- 229920000669 heparin Polymers 0.000 claims description 8
- 229960002897 heparin Drugs 0.000 claims description 8
- 239000000872 buffer Substances 0.000 claims description 2
- 239000012556 adjustment buffer Substances 0.000 claims 1
- 238000010979 pH adjustment Methods 0.000 claims 1
- 102000004032 Heparin Cofactor II Human genes 0.000 abstract description 6
- 108090000481 Heparin Cofactor II Proteins 0.000 abstract description 6
- 239000000203 mixture Substances 0.000 abstract description 5
- 239000007853 buffer solution Substances 0.000 abstract description 2
- 239000002131 composite material Substances 0.000 abstract 2
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
- 238000011534 incubation Methods 0.000 description 11
- 238000012360 testing method Methods 0.000 description 10
- 230000009257 reactivity Effects 0.000 description 9
- 239000000523 sample Substances 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 229920002683 Glycosaminoglycan Polymers 0.000 description 6
- 239000004019 antithrombin Substances 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 239000003593 chromogenic compound Substances 0.000 description 5
- 238000002835 absorbance Methods 0.000 description 4
- 208000007536 Thrombosis Diseases 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 101100450563 Mus musculus Serpind1 gene Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000000691 measurement method Methods 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 235000002639 sodium chloride Nutrition 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- IMRYETFJNLKUHK-UHFFFAOYSA-N traseolide Chemical compound CC1=C(C(C)=O)C=C2C(C(C)C)C(C)C(C)(C)C2=C1 IMRYETFJNLKUHK-UHFFFAOYSA-N 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 108010039209 Blood Coagulation Factors Proteins 0.000 description 1
- 102000015081 Blood Coagulation Factors Human genes 0.000 description 1
- 102000009123 Fibrin Human genes 0.000 description 1
- 108010073385 Fibrin Proteins 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 229940122388 Thrombin inhibitor Drugs 0.000 description 1
- 239000003114 blood coagulation factor Substances 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- 231100000021 irritant Toxicity 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007310 pathophysiology Effects 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000003868 thrombin inhibitor Substances 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Neurosurgery (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4003284A JPS60185163A (ja) | 1984-03-02 | 1984-03-02 | 抗トロンビン物質の測定法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4003284A JPS60185163A (ja) | 1984-03-02 | 1984-03-02 | 抗トロンビン物質の測定法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60185163A true JPS60185163A (ja) | 1985-09-20 |
| JPH0518559B2 JPH0518559B2 (enrdf_load_stackoverflow) | 1993-03-12 |
Family
ID=12569567
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4003284A Granted JPS60185163A (ja) | 1984-03-02 | 1984-03-02 | 抗トロンビン物質の測定法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS60185163A (enrdf_load_stackoverflow) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999038997A1 (fr) * | 1998-01-30 | 1999-08-05 | Yoshitomi Pharmaceutical Industries, Ltd. | Procede de mesure de l'activite du cofacteur ii de l'heparine et boite de reactifs y relative |
-
1984
- 1984-03-02 JP JP4003284A patent/JPS60185163A/ja active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999038997A1 (fr) * | 1998-01-30 | 1999-08-05 | Yoshitomi Pharmaceutical Industries, Ltd. | Procede de mesure de l'activite du cofacteur ii de l'heparine et boite de reactifs y relative |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0518559B2 (enrdf_load_stackoverflow) | 1993-03-12 |
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