JPS60183560A - Automatic analyzing device - Google Patents

Abstract

PURPOSE:To analyze concentration efficiently with high accuacy by calculating the optical path length of a reactor previously, putting liquid to be inspected in the reactor and performing concentration measuring operation by the automatic conveyance of the reactor, and correcting the measured value of concentration with the optical path length of the reactor. CONSTITUTION:Numbers of reactors 3 are moved intermittently by a conveying means 2. Then, the reactors 3 are cleaned at a position (a) and pure water is put in the reactors 3 at a position (b); and they are irradiated with light with two absorption wavelength in a near infrared-ray wavelength range from an irradiating mirror 21 and the optical path length of the reactors 3 is calculated from different in light absorption and inputted to a storage device 30. Then, the water is discharged at a position (c) and the liquid 7 to be inspected is dispensed at a position (d). The reactors 3 are conveyed automatically in sequence and the liquid is irradiated at a position (e) through an irradiating mirror 22 for concentration measurement to measure the concentration, which is inputted to an operator 27. Then, the measured value of the liquid concentration is corrected as to the influence of the optical path length of the reactors which is stored in advance and displayed 28. Therefore, the measured value of the liquid concentration is only corrected with the optical path length of the reactors in series operation to take an analysis efficiently with high accuracy.

Description

DETAILED DESCRIPTION OF THE INVENTION (a) Industrial Application Field This invention relates to an automatic analyzer, and relates to a white movement (h) device that automatically measures the constituent warm stones of a large number of test samples in a QW. .

(b) In conventional automatic analyzers, since it is cumbersome to automatically create the optical path lengths of the many reaction vessels used with the same accuracy, it is necessary to compensate for the optical path length of each reaction vessel. It is very difficult to start the measurement for a long time (KF required).

What is the optical path length of the reaction vessel that also serves as a measurement cell? +li, iL
As disclosed in 1197-1 (I7942), a colored solution is added to a large number of reaction vessels (the average of the measured absorbances is taken as the standard absorbance, and the average of the measured absorbance is taken as the standard absorbance, and the Compare the absorbance measured using water with the absorbance of It is done -C.

However, when using this method, (1) In order to know the optical path length of the reaction vessel (1), calculate the absorbance of two types of liquids, one colored with a dye and one water.
Is it necessary to measure twice?

(2) When selecting a dye that can be used to color a solution,
It is required that the light color is stable and that it does not adsorb to the reaction vessel and contaminate it.

(The relationship between the measured value of the absorbance of the d-body and the optical path length must be changed to the optical path (k) of the reaction vessel.

The measurement of the optical path length of the reaction vessel could not be carried out on the 11th day of the 14th day of the test, since the sample was being folded during the trial period.

There were other inconveniences.

(c) Purpose of the Invention This invention was made in view of the above-mentioned quasi-j1 circumstances, and one of its main purposes is to correct the optical path length of a reaction vessel by using a stable liquid lubrication system that does not use dyes. An object of the present invention is to provide an automatic analyzer that can simultaneously measure a test component using the same method.

(D) Structure of the Invention The present invention comprises a large number of reaction vessels, a means for transporting them, a liquid for increasing the optical path length by 9% in total, a predetermined amount of reagent 1, and an analytical reagent into each reaction vessel. The dispensing means for dispensing the liquid and each reaction vessel into which the 31 specimens were dispensed for determining the optical path length were irradiated with light with two absorption wavelengths in the near-infrared wavelength range. i! into each reaction vessel into which 1 was dispensed. An irradiation means for irradiating wavelength light for 3 degree measurement, two measurement stages for measuring the irradiated dual absorption wave light, and 61 times the optical path length of each reaction vessel is memorized from the measurement results. The irradiated measurement wave f (measures 1B11 degrees of the component to be detected from the light) measurement means, the word 1 (I11 means C measurement (
For each measurement performed (in addition, the compensation of C for the optical path length of each reaction vessel is stored in the compensation memory means (a compensation compensation means with a compensation depth of 91 mm) and the operation of each of the ten means. System 1211!
It is equipped with J61'l 1311 means/J automatic analysis equipment.

(E) Embodiment 1. This invention will be further described based on one embodiment with reference to the drawings. However, this invention is not limited by this.

In Figure 1, the automatic analyzer (1), which uses direct photometry in reaction vessels, has a large number of 50-diameter vessels (3) that also serve as measurement cells.
, (3)... intermittently giFII? Dispense the water ((5), sample (7) and water (6), reagent !81 +9) for measuring the optical path length into each reaction container (3). Dispensing means! /11 and the water ((5
) is dispensed into each reaction vessel (3), and the test sample (7) is irradiated with light with two absorption wavelengths in the near-infrared wavelength range. 1
The irradiation means (101) receives the set wavelength light from the irradiation means (101) and irradiates each reaction vessel (33) with a wavelength light for measuring the concentration of the reaction solution. (Measure the optical path length and concentration of each reaction vessel (3) t′j,′lA
The analyzer (1) is provided on the measurement side for correcting -(1-j) for the optical path length of each reaction vessel (3).

Conveyor 1 (2) (In the example shown in the figure, a conveyor for transporting -1 and 1 is shown. .

Dispenser Pj +41 Nia3iT IJ:, Water (53)
, (01a5 Yohi reagent to+, (9) is dispensed using dispensers 02), fl:3+, (+4), and (15), respectively, and placed on the sample mounting table (161-l). Dispensing the sample sample (7) that has been removed is completed using the ivy (17). In this example, reagent (8), +
9), the case where two types of reagents (ε
3), (53) are limited to 2 Todo swords] (not measured), and are increased or decreased according to 8 east, such as j'11-1.

The irradiation means 00) has a light source run/(1δ)), 1 nonce (
1st), mirror formed in J diagonal carving, optical path 1
N measurement light source 1: equipped with a jJ Ill mirror (21), a light source IJij mirror (Z21, and a plurality of Noil Ku+24 for transmitting light of each required wavelength) for measuring the concentration of the analyte component, and maintained so as to rotate 11. , and the removed Norter rack installation I-ta (A). Filter rack installation
- Evening I? 3+ 1.1, water absorption II)! Transmit wavelength light J
A computer equipped with a filter that transmits the wavelengths used for the so-called measurement of various analyte components is installed at a rate of 1 minute. ).

Near-infrared wave 1 (in the region A3) C, which is used to select the absorption wavelength of water, is the same wave I (in the region J3)
(W! Figure 2) and water permeability (Figure 3) -C
It is necessary to select three wavelengths that cancel out the absorption of the whites in the reaction vessel (3), and transport 1 of 3) into the reaction vessel (3).
Using the air between the reaction vessels (33) in 1.1 as a reference, the absorbance between the two liquid lengths is determined to be zero. For example, a reaction vessel made of quartz (
33) is shown in Fig. 2, 900nm-2100
In the wavelength range of nm, C shows a constant transmittance characteristic, so the three wavelengths for measurement are /11/λ,...
= 900nm/9751101.1(170nm/
12001'1ll, 11070n/1260
On the other hand, it is possible to use a reaction vessel made of synthetic resin [31].There are many absorption bands specific to synthetic resins in the near-infrared wavelength region. [
requires 8 laziness. For example, if you select Acnol resin as the synthetic resin flu, 94
0~9g0n…, 10Go, ~・+NOI1m, 12
[i0□~+310 n Since it shows a constant transmittance in the wavelength range of 1, the three wavelengths for measurement are Cλ1/λ, = 9
7flnm/+070nm or 11070n
/ 428 (The use of lnm is shocking.

The analysis section (11) receives irradiation of three wavelengths from IC Teru Q-1 Kawa Mihu (21) (optical path length measurement), and each reaction vessel (3
3) Measuring means (c) for measuring the optical path length;
old system means Qb for measuring the pox in each reaction vessel (3) by receiving irradiation at the measurement wavelength from the measurement wavelength irradiation unit (3);
), and for each reaction vessel (3) stored in to calculate the corrected temperature using the correction calculation means (271).
3) control means for controlling the operation of each means of the device configuration;
Tokara Natsu-C is here.

Reaction liquid after measurement and water after optical path length measurement (ji)
I, 1 volume is discharged from each reaction vessel (3) by the discharge device.

Next, I will explain the operation of the device.

When the conveyance stage J (2), which is moved intermittently, stops,
The test component is a3 at position (0)! The reaction solution in the reaction container (3
1) 1 is output outside the device. Next minute? U-:S (12)
Yotsu (Water of constant ω (I11! water) (5) is inserted into the needle. 70 degrees This washing water (water) is IJt-!I!) i'
Take out υ1 to the outside of the UH position using Z(I), and add 1 new wash water. This operation is repeated several times. This reactor (3) is moved to the dI+ position by means of It was moved to and then stopped.
The light from the light source run 108) is illuminated by placing each filter G41 in front of the reactor (3) through three wavelengths suitable for optical path length measurement. 1. From the two-wave illumination, calculate the optical path length of the reaction vessel (33) by 4 degrees and memorize it.
Absorbance measured at two absorption wavelengths; l is canceled by the reaction vessel, and therefore shows a value proportional to only the water in the optical path length, and by taking that 1lri to determine the accurate optical path length (?lli correct Jru) Kotoka-C Kiruneri. (C)
The water (5) is in the reaction vessel (3) stopped at the position of 1
The prisoner is removed from the stomach by the prisoner. When the reaction container (3) stopped at the small position, the sample i, +(' A fixed amount of A'l +7+ is dispensed by the sample pipette Q71.The test sample (7) is inserted into the reaction vessel (3), and the reaction vessel (3)) is placed in the reaction vessel (3)) for 1θ degree measurement. is moved to position 1iff (0). 1. During the preparation, add reagent (E3) and -C as necessary.
Reagent (0) or minutes? 1. A fixed amount of fl is supplied to each of the containers (14) and 051.

A filter was installed on the reaction liquid in the reaction container (33) which was stopped at the concentration measurement position (e) (°P).
f: Light 3j+ that passed through filter 4) for twice measurement
The wave I of the i lamp (18) (light is irradiated, and the concentration of the analyte component in the reaction solution is measured by the old control means IAj) I-2. 4 Measurement means G: & l 'c measured depth general measurement〉1j value includes the effect of the optical path length on the reaction vessel (3); 7θ (j3 C1 ahead (
The reactor i! stored in the J-actual storage means (c). i; f
:31 optical path 1, (J and C are removed to correct the absorbance and converted to the same optical path length) the degree of closure is increased by 1'j.

The above operation causes J, C11f7 to be sent to the conveying means.
(< 3 for each reaction vessel), 1211 columns 1 (
x, i) is J, one (restricted).

I, using the device described in 9.1 (well, using the '81〈Noura'' l color method for the light of each anti-1 Shintani device (33), 4
Is it possible to perform only E (5) in f, but test 2.il (how does the measurement of the test component in 7+ ++,%?J or c').For this reason, By solving the technical and economical inconveniences of the optical path jma (equipped with a constant number of anti-1.L containers in the J method),
Row mukai juril kotka C', C! Nil. ,1,ta,reaction'
6:;g f3) (J) Since the molding L'lI is easy to avoid the stagnation and can be reduced to 4τ, the size of the reactor 8 is reduced to 1°j, and the amount of reaction liquid (test run) is reduced. iii ・FA body j
+! ) (1) Necessary M jii f small% < LI
T If :+'j 0 month) i) Is it to cover the decrease?

4 丙13, I', ', ii[: In the example, C3 is
Optical path of reaction vessel] (Although we have explained the case where water is used for measurement, it is also necessary to separate
↓You can also measure the light path length of the C-I core container by dispensing ix and 411 reagent components, i.

<l\) The effectiveness of invention! ,! ! In this invention, water or 5mjl'+'dli is used to correct the optical path length of the reaction vessel, and the optical path length is measured based on the concentration of the analyte component (1) i'll! -C using the same equipment
Same 11. 'l Is this series of operations efficient? -J (J to do J, U (J to 6U) The reaction 'dl j (1's small 111
Realization CJ~1゜4 Simplified explanation of the drawings Figure 1 (A> is a small explanatory diagram of one embodiment of this invention, Figure 1 (13) is a diagram showing the installation of the filter rack. -Explanatory plan view of the computer, Figure 2 (1 quartz and acrylic (611 fingers))
Figure 3 is a diagram showing the Δ penetration rate, Figure 3 is a diagram showing the water permeability when using an anti-I core '8 device with the +trj arrow, Figure 4 is 1,
Then, there is a small lJ diagram C showing the relationship between the optical path length and the absorption trace of water when using 6E as the container for each measured three-wave combination μ.

Claims (1)

[Claims] 1. A large number of reaction vessels, their conveyance means, and an optical path length of 31
Constant ffH of liquid, test sample and analytical reagent
a dispensing means for dispensing the liquid into each reaction vessel, and a dispensing means for dispensing the liquid into each reaction vessel, into which the liquid for measuring the optical path length is dispensed,
Irradiation means that irradiates light with dual absorption wavelengths in the near-infrared wavelength range and irradiates wavelength light for concentration measurement onto each reaction container into which the test sample is dispensed, and measures the irradiated light with dual absorption wavelengths. a measuring means for determining the optical path length of each reaction vessel based on the measurement results and storing the optical path length for each reaction vessel; I means and the i1 measurement means U'1Ill supplement the optical path length of each reaction vessel stored in the storage means for each determined measurement value? 11 control means for controlling the operation of each of the above means, such as a correction force control means for recommending supplementary temperature.
Automatic analyzer. 2. The automatic analyzer according to claim 1, which also serves as a reaction vessel and a measurement barrel. 3. A reaction vessel or a reaction vessel for adjusting the reaction solution;
The white product according to claim 1, further comprising a measurement rail for measuring the resulting reaction solution. 4. The automatic analyzer according to item 1, which is a liquid used to measure the optical path length, pure water or a buffer solution.