JPS60166845A - Diagnosis of cancer - Google Patents
Diagnosis of cancerInfo
- Publication number
- JPS60166845A JPS60166845A JP69885A JP69885A JPS60166845A JP S60166845 A JPS60166845 A JP S60166845A JP 69885 A JP69885 A JP 69885A JP 69885 A JP69885 A JP 69885A JP S60166845 A JPS60166845 A JP S60166845A
- Authority
- JP
- Japan
- Prior art keywords
- cancer
- specimen
- shutter
- fluorescence
- polarized
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6445—Measuring fluorescence polarisation
Landscapes
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
【発明の詳細な説明】 本発明はガン診断方法に関するものである。[Detailed description of the invention] The present invention relates to a method for diagnosing cancer.
最近被検者より採取した血液にガン細胞より取出した蛋
白を混ぜたものをＦ Ｄ Ａ （Ｆｌｕｏｒｅｓｃｅｉ
ｎｄｉａｃｅｔａｔｅ ）にて染色し、これに偏光した
励起光をあて螢光を発せしめ、この螢光の偏光度を測定
することによってガンの診断を行なう方法が発見された
。それはガン患者のリンパ球はガン抗原（ガン蛋白）に
よって特異的に反応し、リンパ球がこの抗原或いはこれ
と類似の物質に接すると何等かの刺激を受ける。そして
このリンパ球の刺激によって起こされる細胞質の変化が
極めて短時間に細胞の螢光偏光という物理的性質の変イ
１として検出されることによる。従って、前述のように
被検者の血液からリンパ球だけを分離しこれにガン蛋白
を混ぜたものをＦＤＡで染色しこれに偏光した励起光を
あてた時に発する螢光のうち励起光と同じ振動方向の成
分を１，１、これと垂直の振動方向をもつ成分を１□と
すると
１、、＋ＱＩよ
で表わされる螢光偏光度Ｐをめれば良い。この螢光偏光
度Ｐの値は正常人では１．１９〜１．５９であるのに対
し、ガン患者では０．６６〜０．８６である。そしてこ
の方法は蛋白を変えることにより被検者がガン患者の場
合、ガンの種類も判定することが出来る。A mixture of blood recently collected from a patient and proteins extracted from cancer cells is used by FDA (Fluorescei
A method has been discovered for diagnosing cancer by staining with niacetate and emitting fluorescent light by exposing the dye to polarized excitation light and measuring the degree of polarization of the fluorescent light. Lymphocytes of cancer patients react specifically with cancer antigens (cancer proteins), and when lymphocytes come into contact with this antigen or a similar substance, they receive some kind of stimulation. Changes in the cytoplasm caused by this stimulation of lymphocytes are detected in an extremely short time as changes in the physical properties of the cells, such as fluorescence polarization. Therefore, as mentioned above, when lymphocytes are separated from the subject's blood, mixed with cancer proteins, and stained with FDA, polarized excitation light is emitted, which is the same as the excitation light. If the component in the vibration direction is 1,1, and the component in the vibration direction perpendicular to this is 1□, then the fluorescence polarization degree P expressed as 1, . . . +QI can be calculated. The value of the fluorescence polarization degree P is 1.19 to 1.59 in normal people, while it is 0.66 to 0.86 in cancer patients. If the subject is a cancer patient, this method can also determine the type of cancer by changing the protein.
本発明は上述の発見をもとにして、試料に偏力した励起
光をあて螢光を生ぜしめ、この螢光の偏光度を電気的に
処理することによってガンであるか否かの診断を自動的
に行ない得るようにしたガン診断一方法を提供するもの
である。Based on the above-mentioned discovery, the present invention is capable of diagnosing cancer by applying polarized excitation light to a sample to generate fluorescence, and electrically processing the degree of polarization of this fluorescence. The present invention provides a method for automatically diagnosing cancer.
以下本発明方法の詳細な内容を説明すると、図面は本発
明方法に用いるガン診断装置を示しており、１は光源、
２はコレクターレンズ、３はシャッター、４はコンデン
サーレンズでこれらで一般の観察用透過照明系を構成す
る。５は試料、６は対物レンズである。又７は超高圧水
銀灯等よりなる螢光測光用光源、８はコレクターレンズ
、９はシャッター、１０は偏光子、１１は励起フィルタ
ー、１２は励起光を反射し螢光を透過するような特性を
有するグイクロイックミラーで対物レンズ６を含めこれ
らで落射螢光偏光測光用照明系を構成し、偏光した励起
光にて対物レンズ６により試料５を照明する。又１３は
励起光を吸収する吸収フィルター、１４は半透過プリズ
ム、１５は接眼レンズ、１６はウォラストンプリズム等
の検光子、１７ａ、１７ｂは受光素子、１８ａ、１８ｂ
は増幅器、１９は演算処理回路、２０は表示装置である
。To explain the details of the method of the present invention below, the drawing shows a cancer diagnostic device used in the method of the present invention, and 1 is a light source;
Reference numeral 2 is a collector lens, 3 is a shutter, and 4 is a condenser lens, which constitute a general transmitted illumination system for observation. 5 is a sample, and 6 is an objective lens. Further, 7 is a light source for fluorescence photometry such as an ultra-high pressure mercury lamp, 8 is a collector lens, 9 is a shutter, 10 is a polarizer, 11 is an excitation filter, and 12 has a characteristic of reflecting excitation light and transmitting fluorescent light. The guichroic mirror including the objective lens 6 constitutes an illumination system for incident fluorescence polarization photometry, and the sample 5 is illuminated by the objective lens 6 with polarized excitation light. Further, 13 is an absorption filter that absorbs excitation light, 14 is a semi-transparent prism, 15 is an eyepiece, 16 is an analyzer such as a Wollaston prism, 17a and 17b are light receiving elements, and 18a and 18b.
19 is an arithmetic processing circuit, and 20 is a display device.
このよ・）な光学系において、前述のようなガンか否か
を判定すべき試料をおき、まずシャッター３を開き、シ
ャッター９を閉じた状態で、試料を観察透過照明系にて
照明し、接眼レンズ１５にて観察しながら対物レンズの
ピント合わせを行ない又、試料中の測光すべき部分を定
める等する。次にシャッター３を閉じシャッター９を開
いて落射螢光偏光測光照明系による照明を行なう。これ
によって偏光された励起光にて照明された試料は螢光を
発し、この螢光はウォラストンプリズム１６を介して受
光素子１７ａ、１７ｂによりその直交する二つの偏光成
分が受光され、増幅器１８ａ。In this optical system, a sample to be determined as to whether it is a cancer or not as described above is placed, first the shutter 3 is opened, and with the shutter 9 closed, the sample is illuminated by the observation transmitted illumination system, While observing through the eyepiece 15, the objective lens is focused, and the portion of the sample to be photometered is determined. Next, the shutter 3 is closed, the shutter 9 is opened, and illumination is performed using an epi-fluorescence polarization photometric illumination system. The sample illuminated with the polarized excitation light thereby emits fluorescent light, and the two orthogonal polarized components of this fluorescent light are received by the light receiving elements 17a and 17b via the Wollaston prism 16, and then sent to the amplifier 18a.
１８ｂ、演算処理回路１９を通して前述の偏光度Ｐが演
算され、ガンであるが否がか判定され、その結果が表示
装置２ｏに表示される。18b, the aforementioned degree of polarization P is calculated through the arithmetic processing circuit 19, it is determined whether or not it is cancer, and the result is displayed on the display device 2o.
以」二説明したように本発明のガン診断方法によれば、
その結果が自動的に表示されることがら未熟練者でも行
えることは勿論、顕微鏡による測光を行なうので、試料
は極めて微量で良く、ガンの集団検診に用いれば極めて
有効である。又観察用照明系及び螢光偏光測光用照明系
に夫々シャッターを設けて、簡単な操作で両照明系を切
換え使用するようにしたので、例えば励起光による試料
の照明は測光時のみ行なうことが出来螢光消光による影
響を防止し得ると共に測光時観察照明光からの励起光成
分による影響を防止し得る等、精度の高い判定が可能で
ある。As explained below, according to the cancer diagnosis method of the present invention,
Since the results are automatically displayed, it can be performed even by unskilled people, and since photometry is performed using a microscope, only a very small amount of sample is required, making it extremely effective when used in mass cancer screenings. In addition, a shutter is provided for each of the observation illumination system and the fluorescence polarization photometry illumination system, so that both illumination systems can be switched and used with a simple operation, so, for example, the sample can be illuminated with excitation light only during photometry. It is possible to make highly accurate determinations, such as being able to prevent the effects of quenching of fluorescent light and the effects of excitation light components from the observation illumination light during photometry.
尚、上記ガン診断装置では測光照明系として落射照明を
用いているが、透過照明にすることも可能である。又検
光子にはウォラストンプリズムを用い二成分に分割して
両成分を検出しているカベ検光子を９０°回転させこれ
と受光素子とを同ｊｊＪＪさせることにより両成分を検
出するようにしても良い。又、螢光が弱いために受光素
子としては高感度のものが用いられるが、この受光素子
に強い観察用の照明光が入射することは好ましくない。Incidentally, although epi-illumination is used as the photometric illumination system in the cancer diagnostic apparatus described above, it is also possible to use transmitted illumination. In addition, a Wollaston prism is used as the analyzer, and the wall analyzer is divided into two components and both components are detected.The wall analyzer is rotated 90 degrees and this and the light receiving element are aligned in the same direction to detect both components. Also good. Furthermore, since the fluorescent light is weak, a highly sensitive light-receiving element is used, but it is undesirable for strong illumination light for observation to be incident on this light-receiving element.
従って受光素子の前方適宜位置にもツヤツタ−を設け、
測光の時だけ光が受光素子に入射するようにしても良い
。Therefore, a gloss is provided at an appropriate position in front of the light receiving element,
Light may be made to enter the light receiving element only during photometry.
図面は本発明方法に用いるガン診断装置の構成を示す図
である。
■・・・・光源、３・・・・シャッター、５・・・・試
料、６・・・・対物レンズ、７・・・測光用光源、９・
・・・シャック−１１０・・・・偏光子、１１・・・・
励起フィルター、１２・・・・グイクロイックミラー、
１６・・・・検光子、１７ａ、１７ｂ・・・・受光素子
、１９・・・・演算処理回路、２０・・・・表示装置。The drawing is a diagram showing the configuration of a cancer diagnostic device used in the method of the present invention. ■... Light source, 3... Shutter, 5... Sample, 6... Objective lens, 7... Light source for photometry, 9...
...Shack-110...Polarizer, 11...
Excitation filter, 12... Gicroic mirror,
16... Analyzer, 17a, 17b... Light receiving element, 19... Arithmetic processing circuit, 20... Display device.
Claims (2)
た試料を作成し、上記試料を偏光した励起光により照明
しつつ上記試料より発した螢光偏光を受光素子で測定し
、上記受光素子からの電気信号をもとに演算回路により
螢光偏光度を算出して該螢光偏光度が所定値であるか否
かによりガンであるか否かを判定し、その判定結果を表
示装置により表示するようにした、ガン診断方法。(1) A sample is prepared by mixing cancer cell proteins with lymphocytes of the subject, and while illuminating the sample with polarized excitation light, the polarized fluorescent light emitted from the sample is measured with a light receiving element. Based on the electric signal from the element, a calculation circuit calculates the degree of polarization of the fluorescent light, determines whether or not it is cancer based on whether the degree of polarization of the fluorescent light is within a predetermined value, and displays the determination result on a display device. A method for diagnosing cancer that is displayed using
合正常であると判断し、該値が０．６６〜０．８６であ
る場合ガンであると判断するようにしたことを特徴とす
る特許請求の範囲（１）に記載のガン診断方法。(2) If the value of the degree of fluorescence polarization is between 1.19 and 1.59, it is determined to be normal, and when the value is between 0.66 and 0.86, it is determined to be cancer. The cancer diagnostic method according to claim (1), characterized in that:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP69885A JPS60166845A (en) | 1985-01-07 | 1985-01-07 | Diagnosis of cancer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP69885A JPS60166845A (en) | 1985-01-07 | 1985-01-07 | Diagnosis of cancer |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP52036200A Division JPS5913697B2 (en) | 1977-04-01 | 1977-04-01 | Cancer diagnostic equipment using a fluorescent polarization photometric microscope |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60166845A true JPS60166845A (en) | 1985-08-30 |
| JPS6215820B2 JPS6215820B2 (en) | 1987-04-09 |
Family
ID=11480984
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP69885A Granted JPS60166845A (en) | 1985-01-07 | 1985-01-07 | Diagnosis of cancer |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS60166845A (en) |
-
1985
- 1985-01-07 JP JP69885A patent/JPS60166845A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6215820B2 (en) | 1987-04-09 |
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