JPS6015885B2 - Automatic colorimetric determination device for electrophoresis - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to an automatic colorimetric determination apparatus for use in turtle electrophoresis.

BACKGROUND ART Conventionally, the string air phoresis method has been used in clinical laboratories and the like for fractional quantification of serum proteins.

As is well known, in this electrolacquering method, the serum to be tested is coated on a support made of cellulose acetate paper, etc., an image is formed of the serum by applying electricity, and further steps such as staining and decolorization are carried out. , which performs colorimetric quantification of minute images, but each of these steps is extremely inefficient because all of these steps are performed manually. In order to eliminate these drawbacks, improve work efficiency, and always ensure accurate measurements, we have developed a method that can automatically perform each step of the turtle migration method in sequence, as described above. A laying device is desired. The present invention has been made based on the above requirements, and is capable of transparentizing a support that has been stained, decolorized, and further dried after electrophoresis, and sequentially automatically quantifying images of each serum on the support. The present invention provides a colorimetric method for determining rhododendron.

DESCRIPTION OF THE PREFERRED EMBODIMENTS The details of the automatic colorimetric determination apparatus of the present invention will be explained below based on the illustrated embodiments.

In the drawings, reference numeral 1 denotes a main body of the colorimetric determination apparatus, and a liquid tank 2 in which one recessed part 2a and 2b are formed is provided on the upper part. A clearing liquid 3 such as liquid paraffin or decalin is placed in a concave portion 2a of the concave portions of this liquid tank 2. Further, an opening is formed in the center of the liquid tank 2, and a transparent glass plate 4 is placed here. 5 is a holding plate placed on the top of the liquid tank 2, and a glass plate 4 is placed in the center of the holding plate.
Another transparent glass plate 6 is arranged at a position facing the.

Further, the structure is such that a small gap is formed between the holding plate 5 and the liquid tank, allowing the support 10 to pass therethrough. Further, a hole 5a is formed in the holding plate 5 so that a light receiving element and the like can be moved as described later. 11 is four sets of rollers 13, 14: 15, 16: 17, 18: 19, 20
A notch 12a is formed in the side plate 12 of this support box as shown in FIG. The support box 11 is placed on the main body 1 using rollers 7 and 8, and four sets of rollers 13, 14:15, 16:17,
18; 19, 20 are positioned as shown.

Also, among these four sets of low-fu, rollers 13, 15, 17
, 19 have gears 21, 22 at one end of the shafts 13a, 15a, 17a, 19a as shown in FIGS. 2 and 3.
, 23, 24 are fixed. 25 is a transport motor installed on the roller support box 11, 27 is a gear fixed to the rotating shaft 26 of the motor 25, and 28 is a shaft of this gear 27 and each of the rollers 13, 15, 17, and 19. A chain connecting gears 21, 22, 23, and 24 fixed to 13a, 15a, 17a, and 19a to simultaneously rotate four sets of rollers; 301; a light source section of an optical system for colorimetric light; Inside thereof, optical elements such as a light source 31, a condenser lens 32, a prism 33, etc. are arranged.

34 is a light emitting element fixed to main body 1, 35 is support body 10
A light-receiving element is fixed to the holding plate 5 opposite to the light-emitting element 34 across the gap between the two transparent glass plates 4 and 6 through which the light-emitting element 34 and the light-receiving element 35 are positioned. It constitutes a mechanism for

Reference numeral 36 denotes a light receiving element for measurement, which is attached to a support plate 37 so as to receive measurement light from a light source that has passed through the slit 2c, and which has passed through the support 10. Attach both ends of the light source section 30 with appropriate fixing means.
11 are fixed.

38 and 39 are shafts that pass through the light source section 30 and are fixed to the main body 1; 40 and 41 are sliding members that are iron-fitted so that they can slide on the shafts 38 and 39, respectively; A rack 42 is fixed to the side surface of the sliding member 40.

43 is a main scanning motor, and 44 is a pinion that is fixed to the rotating shaft of the main scanning motor and meshes with the rack 42.

Therefore, when the main scanning motor 43 is operated, the rack 42 is moved by the rotation of the pinion 44, and the light source section 30 is moved along the shafts 38, 39 with the support plate 37.
1 in a direction perpendicular to the paper surface of FIG. Next, in Figs. 3 and 4, there are five sub-scanning motors,
A lever 52 has one end fixed to the rotating shaft 51 of the motor 50, and a roller 53 is attached to the tip of the lever. Another lever 54 is connected to the shaft 15a of the roller 15 via a one-way transmission clutch (not shown).

Reference numeral 55 denotes a spring so that the lever 54 is always in contact with the roller 53 provided at the tip of the lever 52, and 56 is a limit switch.

With this structure, the rotation of the sub-scanning motor 50 causes the lever 52 to rotate around the shaft 51, and the rotation of this lever 52 causes the tip of the other lever 54 to reciprocate around the shaft 15a. . Based on this reciprocating movement of the lever 54, the shaft 15a and therefore the roller 15 rotate by a certain amount in one direction. Next, the operation of the entire colorimetric determination apparatus having the configuration described above will be explained. First, the support 10 that has been dyed and decolorized and further dried is fed into the quantitative apparatus of the present invention from the left side of FIG. through four sets of rollers 13, 14; 15,
16: 17, 18; 19, 20 are rotated at the same time.
By the rotation of this roller, the support body 10 is sequentially moved from the left to the right in FIG. When the support 10 passes between the rollers 15 and 16 due to this movement, the support 10 is coated with the clarifying liquid by the roller 16 soaked in the clarifying liquid 3 such as liquid paraffin or decalin. 3 is attached and uniformly made transparent. In this case, if a wet material such as a sponge is used as the roller 16, the adhesion of the clarifying liquid to the support becomes more reliable. When the support 1 is further moved to the right, it is inserted into the gap between the two transparent glass plates 4 and 6 and advances between them. When the tip enters between the light emitting element 34 for position detection and the light receiving element 35, a change in the amount of light reaching the light receiving element 35 is detected, and a signal based on this is used to control the transport motor 25. When the operation is stopped, the support body 10 stops at that position. In this case, since the support 10 is made transparent by the transparentizing liquid 3, the change in the amount of light is extremely small, and therefore it is considered difficult to detect. In order to lower this, support 10
It is desirable to provide an opaque mark in advance at the tip of the mark. When the support 10 is stopped at a predetermined position in this way, the main scanning motor 43 starts operating, and the pinion 44 and rack 42 move the light source 30 in a direction perpendicular to the plane of the paper (for example, upward in FIG. 2). lower). As the light source section 30 moves, the support plate 37
also move. In this way, the measurement optical system including the measurement light receiving element 36 moves while the support body 10 remains stopped at a fixed position. The light-emitting element 34 and the light-receiving element 35 for position detection and the above-mentioned measurement If you place the optical axis of the optical system in the correct position according to the position where the serum is applied,
It is possible to accurately scan and measure a minute image. Here, the images detected by the light receiving element 36 are sequentially recorded on the recorder. When the measurement of one serum image is completed in this way, although not shown in the figure, the main scanning motor 4 is moved to an appropriate position or by the action of a mitt switch, etc.
3 is reversed to return the light source section 30 and the measuring light receiving element 36, that is, the measuring optical system to their original positions. At the same time, the sub-scanning motor 50 is rotated, and the four sets of rollers are simultaneously rotated by a certain amount via the roller 15 and the chain 28 as described above, and the support body 10 is advanced by a certain amount. . If the amount of rotation of the roller at this time is set to an amount equivalent to moving the support by the gap between the images of each serum applied on the support, the operation of the motor 50 for sub-scanning can be As a result, the second serum fraction image reaches the optical axis of the measuring optical system. If the main scanning motor 43 is operated again here, the second serum fraction image can be measured. In this way, the minute images of each serum applied onto the support are measured one after another, and after the measurement of all the minute images is completed, the transport motor is operated again to send the support out of the colorimetric determination device. . During this measurement or when the support is transported out of the apparatus, for example when the support passes between the rollers 17 and 18, excess clarifying liquid may drip, so a recess 2b is formed in the liquid tank 2. , it is stored in this. As explained above, according to the automatic colorimetric determination apparatus of the present invention, when the support after dyeing, decolorization, and drying is fed into the apparatus, it is moved into the apparatus by the conveying roller, and is moved to a predetermined position by the position detection mechanism. It is stopped at the position.

In this way, the optical system moves while the support remains stationary to perform main scanning, and one minute image is measured. In other words, colorimetric determination of one support can be performed automatically, extremely efficiently and accurately.

[Brief explanation of the drawing]

FIG. 1 is a sectional view of the apparatus of the present invention, FIG. 2 is a plan view, FIG. 3 is a front view, and FIG. 4 is a diagram showing a mechanism for performing sub-scanning. 1... Colorimetric determination device body, 2... Liquid tank, 3... Clarifying liquid, 11... Roller holding box, 13, 14, 15 ,16,17,18,19,
20... Lohu, 21, 22, 23, 24...
...Gear, 25...Transportation motor, 28.
...Chain, 30...Light source section, 34.
... Light-emitting element for position detection, 35 ... Light-receiving element for position detection, 36 ... Light-receiving element for measurement, 43
...Motor for main scanning, 50...Motor for sub-scanning. Figure 1 Figure 2 Figure 3 Figure 4

Claims (1)

[Claims] 1. A means for transporting the support after each step of serum application, electrophoresis, staining, and decolorization to a colorimetric determination device; a position detection optical system for the colorimetric determination device to detect at least the position of the support; An automatic colorimetric determination device for electrophoresis, consisting of a colorimetric measurement optical system that scans a positioned support.