JPS60135836A - Liquid sampler - Google Patents

Abstract

PURPOSE:To enable accurate sampling of a specimen regardless of a film formed on the surface of the liquid specimen by providing a member which moves almost horizontally with the lowering of a suction nozzle to remove a film existing on the surface of a liquid to be sucked. CONSTITUTION:A specimen sampling section starts to lower, advancing a suction nozzle 13 and a working rod 38 into a sample container and then, when it reaches position where the tip of the working rod 38 enters slightly into a clot 2 of blood, a pin 39 abuts against a stopper 40 to halt a fixing base 34 while a nozzle holder 31 continues to lower as intact. Then, with the lowering of a rack 37, a pinion 36 turns counterclockwise and in conjunction, the working rod 38 also turns counterclockwise. With the turning thereof, the fibrin film 4 separated on the clot 2 is pressed against the inner wall of the sample container to remove. Thus, the blood corpuscle alone is sucked and sampled with the suction nozzle 13 being advanced paralleling the turning of the working rod 38.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a sample collecting device suitable for collecting kudzu by suctioning a sample from a liquid sample having a film or the like formed on its surface.

Conventionally, in a sample collection device that applies suction force to a suction nozzle connected to a pump or the like to collect a sample by suction,
If a film is formed on the surface of the sample to be collected, this film becomes a hindrance, causing the inconvenience that the sample cannot be collected accurately.

For example, when collecting blood cells from a blood sample, the blood sample is centrifuged and a suction nozzle is inserted into the blood clot deposited at the bottom of the sample container to extract the blood cells incorporated into the blood clot.
However, as shown in Fig. 1, a feprin film 4 is deposited at the interface between the blood clot 2 deposited at the bottom of the sample container 1 and the supernatant serum 8. It may not be possible to collect accurately. This 7 ebrin membrane 4 is composed of cellulose, and may be a strong membrane that cannot be penetrated by the sample nozzle. This results in the inconvenience of not being able to aspirate. In addition, if the feprin membrane is suctioned along with the blood cells, the suction nozzle will become clogged, resulting in the inconvenience of not being able to collect the blood cells. To provide a sample collecting device configured to be able to accurately collect a sample even if a film is formed on the surface of a liquid sample to be collected, and to prevent clogging of a sample nozzle due to suction of a single film. be.

Summary of the Invention The present invention provides a member that moves approximately horizontally in response to the descent of the suction nozzle to remove the film □ present on the surface of the liquid to be suctioned, and after removing the film, the g&s1 nozzle The film removing member is characterized in that it is configured to protrude below the lower end of the film removing member.

Embodiment FIG. 1 is a diagram showing the configuration of an example of a sample collection device according to the present invention. In this example, a certain amount of the sample in the sample container LO is collected at the sample collection position A, the collected sample is discharged into the sample tube 11 at the sample discharge position B, and the collection nozzle is cleaned at the cleaning position C. It is assumed that the sample collecting section 12 is transferred between the above-mentioned positions by a transfer device (not shown). A suction nozzle 18 and a film removing device are fixed to the sample collecting section 12. The upper end of the suction nozzle 18 connects the solenoid valves 14 and 15 via a tube, and the other end of the solenoid valve 15 connects the cleaning liquid 1.
6 is connected to the cleaning liquid tank 17 in which the cleaning liquid tank 6 is accommodated. A sample syringe 18 for sucking and discharging the sample is connected via a tube between the suction I nozzle 18 and the electromagnetic valve 14, and a cleaning liquid 16 is sent to the G&pull nozzle 18 between the electromagnetic valves 14 and 15. A cleaning syringe 19 for cleaning is connected. Further, a cleaning pump 2o for delivering the cleaning liquid 16 into the cleaning tank 19 is connected to the cleaning liquid tank 17 via a tube.

An opening is provided at the lower end of the cleaning tank 19 , and this opening is connected to a waste liquid tank 22 via a solenoid valve 21 , and the cleaning liquid after cleaning the suction nozzle 18 is disposed of into the waste liquid tank 22 . Further, a dilution nozzle 26'e is connected to the dilution tank 24 containing the dilution liquid 28 via a dilution liquid pump 25, and a sample tube 11 is connected to the dilution nozzle 26'e.
A sample to be analyzed is prepared by diluting the sample dispensed in the diluent 28 with the diluent 28. FIG. The suction nozzle 18 is fixed to the nozzle holder 81. An arm 82 of a transfer device is attached to this nozzle holder 81, and the sample collecting section 12 is configured to move up and down. Further, a pair of guide shafts 88 are attached to the notch 81a of the nozzle holder 81, and a fixing base 84 is slidably attached to the guide shafts 88. Also,
A compression spring 85 is attached to the guide shaft 88 and always biases the fixed base 84 downward. A rack 87 is attached to the nozzle holder 81, and a pinion 85t-' is pivotally attached to the fixed base 84 and meshes with the pinion 85. Binion 8
A crank-shaped actuator $88 is fixed to 6.゛This actuating rod 88 functions to rotate just before the sample nozzle 80 performs the suction 1 operation and remove the phepurin film deposited on the blood clot 2 in the sample container lo, and is in the form of a rod or a plate. Configure. The tip of the actuating rod 8B is set so as to be located below the tip of the suction nozzle 18.

In addition, a bottle 89 is installed on the fixed base 84, and this bottle 89
Attach a stopper 40 to the main body side to cooperate with the stopper 40. pin 3
9 and the stopper 4O, the distance between the bottle 89 and the stopper 4O is
The tip of the actuating rod 88 is set so that it slightly enters the blood clot 2 when it comes into contact with the blood clot 2.

Next, the sampling operation of the sample sampling section 12 will be explained with reference to FIGS. 8 and 4. Sample collection section 1 by transfer device
2 is transferred to the sample collection position A, the sample collection part 1
2 begins to descend, and the suction nostle 18 and the actuating rod 88 enter the sample container 10. When the tip of the actuating rod 88 reaches a position where it slightly enters the blood clot 2, the pin 89 touches the stopper 4.
Contact with 0. At this time, the tip of the suction nozzle 18 is located above the feprin membrane 4. Pin 89 is stopper 40
When the fixed base 84 comes into contact with the nozzle holder 81, the fixed base 84 cannot be lowered and remains in that position against the force of the compression spring 85, but the nozzle holder 81 continues to lower. Therefore, the lowering of the rack 87 causes the pinion a6 to rotate counterclockwise, and the operating rod 88, which is not affected by the rotation of the pinion 86, also rotates counterclockwise.

As shown in FIG. 4, the rotation of the operating rod 88 causes the epurin film 4 deposited on the blood clot 2 to be pressed against the inner wall of the sample container 10 and removed. The suction nozzle 18 continues to advance in parallel with the rotation of the actuating rod 88, and the tip of the suction nozzle
When the sample syringe 18 reaches an appropriate position within the tube, it stops descending, and a suction force is applied from the sample syringe 18 connected via the tube to aspirate the blood cells. In FIG. 4, the solid line shows the state when the pin 89 is in contact with the stopper 40, and the broken line shows the state when the suction nozzle 18 is sucking blood cells. With this configuration, the actuating rod 88 removes the Eprin membrane 4 before the suction nozzle 18 enters the blood clot 2, so the suction nozzle 18 can enter the blood clot 2 without coming into contact with the Eprin membrane 4. , the suction nozzle 18 suctions and collects blood cells without suctioning the feprin membrane.

FIG. 5 is a front view showing a modification of the sample collection section according to the present invention. Components that are the same as those used in FIG. 8 will be described with the same reference numerals. In this example, a rotating shaft 41 is installed on the fixed base 84, and the lever 42 is rotatably supported on the rotating shaft 41. This lever 42 has an operating rod 88.
At the same time, a lock pin 89 is installed, and a stopper 43 that cooperates with this lock pin 89 is attached to the main body side. This stopper 48 has a downward movement stopping surface 48a and a locking pin 89 which is moved horizontally by abutting the locking pin 89.
A horizontal movement stopping surface 48b is formed to stop the horizontal movement of. With this configuration, when the sample collecting section 12 is lowered, the pull pin 89 comes into contact with the lowering stop surface 4B& of the stopper 48, moves horizontally along the lowering stop surface, and the lever 42 moves backward around the rotating shaft 41. The actuating rod 88 rotates counterclockwise as it rotates clockwise. When the four-wheel bin 89 comes into contact with the horizontal movement stop surface 48b, the lever 42 stops rotating, and the operating rod 88 also stops rotating. Even after the rotation of the nozzle holder 814j lever 42 is completed, the nozzle holder 814j further descends by a predetermined distance and then stops. As a result, the suction nozzle 18 enters into the blood clot 2 after the efprin membrane deposited on the surface of the blood clot is removed by rotation of the operating rod 88, and blood cells are collected without suctioning the efprin membrane 4. Ru.

As explained above, according to the present invention, the film formed on the surface of the sample is removed before the suction nozzle enters the sample to be collected, so that the suction nozzle is not obstructed by the film. It is possible to enter the sample without any trouble, and it becomes possible to stably aspirate and collect the sample. Furthermore, since the suction nozzle does not suction the film, clogging of the nozzle can be effectively prevented and the sample can be more stably collected by suction.

[Brief explanation of drawings]

Fig. 1 is a diagram showing the state of a blood sample after centrifugation. Fig. 2 is a diagram showing the configuration of an example of the sample collection device according to the present invention. Fig. 8 is a diagram showing the configuration of an example of the sample collection section according to the present invention. FIG. 4 is a diagram for explaining its operation. FIG. 5 is a front view showing a modified example of the sample collection section. ... Serum 4 ... Feprin membrane 10 ... Sample container 11 ... Sample tube 12 ...
Sample collection section 18... Suction nozzles 14, 15, 2
1... Solenoid valve 16... Cleaning liquid 17... Cleaning liquid tank 18... Sample syringe 19... Washing tank 20... Washing pump 2
2... Waste liquid tank 28... Dilution liquid 24... Dilution liquid tank 25... Dilution pump 26... Dilution nozzle 31...
・Nozzle holder 82... Arm 88... Guide shaft 84... Fixing base 85... Compression spring 86... Pinion 87... Rack 88... Operating rod 39,... Lock pin 40 , 4.8... Stopper, 41... Rotating axis 4! ···lever. Patent applicant: Olympus Optical Industry Co., Ltd. Figure 1 Figure 3 Figure 4 Figure 5

Claims (1)

[Claims] 1 @σ1 A member is provided that moves approximately horizontally in accordance with the descent of the nozzle to remove the film present on the surface of the liquid to be sucked, and after removing the film, the suction nozzle is moved below the lower end of the film removing member. A liquid sampling device characterized in that it is configured to protrude into.