JPS60119456A - Electrophoresis device - Google Patents

Abstract

PURPOSE:To decrease the temp. fluctuation in a migrating tube and to perform migration measurement with high reproducibility by interrupting the operation of a thermostatic means of an electrophoresis device during taking in of the data on the migration speed with said device. CONSTITUTION:A sample liquid is supplied to a migration tube 7 and cooling water is cyclically supplied around the tube 7. When a power source 10 for migration is turned on, a temp. control timing controller 24 upon receiving the signal thereof stops operation of a cooler 21 and closes a stop valve 22 via an actuating part 25 for the stop valve. The sample liquid in the tube 7 is subjected to electrophoresis in the above-mentioned way. Since there is no cyclical supply of the cooling water to a thermostatic chamber 17 during the migration, the temp. of the sample liquid fluctuates less and the measurement with good reproducibility is made possible. A signal 32 for taking in the measurement data is fed to the controller 24 during the time when the measurement data form a data processing part 30 is taken in from a time data memory 28 to an arithmetic analysis part 29 then the interruption of the supply is accomplished by said controller in the case of interrupting the supply of the cooling water.

Description

DETAILED DESCRIPTION OF THE INVENTION (a) Field of Industrial Application This invention relates to an electrophoresis apparatus, and particularly to an electrophoresis apparatus that can reduce fluctuations in temperature conditions during electrophoresis and perform electrophoresis measurements with high reproducibility.

(B) Conventional technology In general, electrophoresis utilizes the phenomenon that when sample ions are caused to migrate by passing an electrophoresis current through an electrophoresis tube, the substances in the sample ions are arranged in fractions due to their different migration speeds. It is something to do.

Therefore, the electrolytic solution in the migration tube generates self-heating corresponding to its electrical resistance and migration current, which inevitably increases the temperature in the migration tube.

In order to suppress this temperature rise, conventional measures have been taken to supply cooling water etc. around the migration tube, but specifically, by intermittent supply of cooling water, the average temperature inside the migration tube increases. is set to a predetermined value (see the set temperature in FIG. 2). In other words, the actual moisture content inside the electrophoresis tube is not constant, but usually changes in a wave-like manner (see the "temperature control" curve in Figure 2), and therefore the temperature conditions during electrophoresis cannot be guaranteed to be the same. There was a problem.

(C) Purpose This invention was made in view of these problems, and one of its main purposes is to provide an electrophoresis device that can reduce fluctuations in temperature conditions during electrophoresis and perform electrophoresis measurements with high reproducibility. It's about doing.

(D) 4t4 This invention comprises a pair of electrode vessels, an electrophoresis tube interposed between these electrode vessels, an electrophoresis power supply, an electrophoresis speed measuring means,
The data processing means is equipped with a data processing means for capturing and processing desired migration velocity data from the measurement data obtained by the measurement means, and a constant temperature means for maintaining the temperature inside the migration tube at a set value, and further, the data processing means includes at least the following: The electrophoresis apparatus is equipped with a control means for interrupting the operation of the constant temperature means when desired migration speed data is acquired.

(e) Examples The present invention will be described in detail below based on examples shown in the drawings. Note that this invention is not limited to this.

FIG. 3 is a diagram showing the overall configuration of the cell electrophoresis device (6).

(The force is the electrophoresis tube, +81+91 is the 2 on the wall end side of this electrophoresis tube.
The electrodes (111) in these electrode reservoirs (00)
This is an electrophoresis power supply connected to f12+. (13) 04
) is the sample liquid feed path and sample liquid drain path, [15106)
is a septum.

07) is a box-shaped thermostatic chamber in which cooling water for cooling the electrophoresis tube (force) is circulated and supplied, and the electrophoresis tube (force is stored and held in this tank.08) (19) is a A cold U1 water D1 wave path and a cooling water supply path extend from the openings in the soil wall and the bottom wall to the outside of the constant temperature bath (17), and these are connected via a cooling tank @) outside the constant temperature bath (17). The water in this cooling tank is
A temperature for maintaining the inside of the migration tube (7) at a preset temperature using a cooler (21) using a refrigeration cycle, e.g.
It is cooled to a temperature around 20°C. In addition, the liquid feeding path 0
3) On the top, an on-off valve and a liquid feeding pump are installed in order from the constant temperature tank 07) side to the cooling tank @).

(g) is the on-off valve operation part (
This is a temperature control timing controller that is electrically connected to the on-off valve &J via the valve controller 1 [] - A) (A). This controller is also electrically connected to the electrophoresis power source m00) and the cooler (21), and is also electrically connected to the electrophoresis power source m00).
In conjunction with the N-OFF signal, the valve control roller (a) is operated, the on-off valve is closed and opened, and the cooling operation of the cooler (21) is stopped (the operation of the refrigeration cycle is stopped). (stop).

Further, (19) is a light irradiation part, 2a is a scattered light detection part,
The detection signal (1+) obtained by this scattered light detection unit is recorded as desired migration data in the data processing unit (30).In other words, the detection signal (1) is amplified and recorded in the time data memory (to). Furthermore, desired data among the recorded data is taken into the calculation analysis section 4 and recorded.

Therefore, first, the sample liquid is supplied to the electrophoresis tube (force), and cooling water is circulated and supplied to the box-shaped constant temperature bath (17), that is, around the electrophoresis tube (force).Next, when the electrophoresis power supply 00 is turned on. ,
Receives that signal (temperature control timing controller □□□
stops the operation of the cooler (21) and instructs the on-off valve (3) to close via the on-off valve actuator (8). In this way, the sample liquid in the electrophoresis tube (7) is subjected to electrophoresis, but at this time, there is no circulating supply (movement) of cooling water to the constant temperature bath (17), so there is little temperature fluctuation of the sample liquid (second (Refer to the "before temperature control" curve in the figure), it is possible to measure with good reproducibility. Furthermore, when detecting scattered light, since the movement of the cooling water is interrupted, there is no risk of air bubbles being introduced as a disturbance, and highly accurate detection is possible. Note that during washing after electrophoresis, cooling water is again circulated and supplied to the constant temperature bath 07). Here, unlike the above embodiment, while the data processing section (30) takes in the measurement data from the time data memory (to) to the calculation analysis section (2), the measurement data is used instead of the signal (31). It is also possible to send the intake signal (32) to the temperature control timing controller (g), thereby interrupting the supply of cooling water as in the case of the signal (31).

(f) Effects This invention interrupts the operation of the thermostatic means at least while capturing migration speed data, so that temperature fluctuations within the migration tube are small and migration measurements with high reproducibility are possible.

[Brief explanation of the drawing]

FIG. 1 is a functional explanatory diagram showing an embodiment of an electrophoresis apparatus according to the present invention, and FIG. 2 is a graph showing temperature changes within an electrophoresis tube. (6)...Cell electrophoresis device, (7)...
...Transfer tube, [81f91... Electrode tank, (10
)......Electrophoresis power supply, (17+...Box-like thermostat, (21)...Cooler, (A)...
・Opening/closing valve, (g)...Temperature control timing controller l-
roller.

Claims (1)

[Claims] 1. A pair of electrode vessels, an electrophoresis tube interposed between these electrode vessels, an electrophoresis power supply, an electrophoresis velocity measuring means, and desired electrophoresis velocity data obtained from the measurement data obtained by this measuring means. a data processing means for processing and a constant temperature means for maintaining the temperature in the migration tube at a set value, and further, the data processing means suspends operation of the constant temperature means at least when acquiring desired migration speed data. An electrophoresis apparatus comprising a control means for controlling the electrophoresis. 2. The electrophoresis apparatus according to claim 1, wherein the migration speed measuring means is an optical measuring means, and the constant temperature means is a cooling water supply means that circulates and supplies cooling water around the migration tube.