JPS5946555A - Reagent feeder for automatic chemical analyzer or the like - Google Patents

Abstract

PURPOSE:To enable the supply of a correct amount of reagent to a reaction tube while preventing mutual pollution of a plurality of reagents with a simple reagent pump by providing a reagent meauring value equipped with a rotational member having a reagent measuring through hole between two fixing members each with a reagent introduction through hole. CONSTITUTION:A first reagent measuring value 31 of an automatic analyzer is so arranged that a rotary member 34 having reagent introduction holes 34a and 34b with a specified correct and equal capacity are arranged between a fixed member 32 having reagent introduction 32a and 32b and a cleaning water introduction hole 32c and a second fixed member 33 having reagent introduction holes 33a and 33b. A pump 35 is connected to the through hole 32a and sucks a reagent from a reagent tank 17a to fill the through hole 34a. A gas is fed into the through hole 33b from a compressor 38 and sends the contents of the through hole 34b to a reaction tube 18 where the reagent has been placed and positioned rotating together with the reagent via a sampling valve 7 from the through hole 32b. This enables the measuring of a reagent accurately and preventing mutual pollution of reagents completely with a simple pump 35.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a reagent supply device for an automatic chemical analyzer, etc., and more particularly to a reagent supply device for an automatic chemical analyzer, etc., which improves the disadvantages of conventional devices in supplying reagents.

FIG. 1 shows a flow path system diagram for conventional automated chemical analysis, in which 1 is a sample tube, and the sample tube 1 contains a sample liquid to be tested. A pipette [-2 for sucking up the sample is inserted into the sample tube 1, and the pipette 2 is connected to the sample 1Q+weighing valve 7, which consists of fixed members 4 and 5 and a rotating member 6 disposed in close contact therebetween. It is connected to a through hole 5a bored in the fixing member 5. The rotary member 6 is provided with at least two through holes 6a, 6b having equal volumes, and each of the fixed members 4.5 has a through hole 4a that can be connected to the through holes 6a, 6b. , 4h, 5a, and 5h are perforated. A switching valve 10 is connected to the through hole 4a of the fixing member 4 via a sampling pump 9, and the switching valve 10 is connected to a cleaning liquid tank 12 via a cleaning pump 11. Now, the switching valve 10 is switched as shown by the solid line, and the sample in the sample tube 1 is sucked up through the pipette 2 by the sampling pump 9, and the sample P13- is introduced into the through hole 6a of the rotating member 6. Also, switching valve 1
0 as shown in the dotted line, move the pipette 2 as shown in the dotted line, suck up the washing liquid from the washing liquid tank 12 using the washing pump 11, and discharge it to the pipette 2. The interior will be cleaned.

The through hole 5b of the fixing member 5 is connected to the first reagent pump 14 via the switching valve 13, and the piping between the valve 13 and the pump 14 is arranged in the preheating tank 15. The pump 14 connects a plurality of first reagent tanks 17a and 17b via a one-channel multi-way switching valve 16.

170. Here, after introducing the sample into the through hole 6a of the rotating member 6 of the sample weighing valve 7, the rotating member 6 is rotated to move the through hole 6a to the position 6b, and in this state, the switching valves 13 and 16 are closed. Switch as shown in the solid line,
When the first reagent lIg 17a is sucked up by the reagent pump 14, the solidified sample can be introduced into the reaction tube 18 together with the first reagent. Note that by switching the switching valve 13 as shown by the dotted line and sucking up the cleaning liquid through the piping 20 with one pump, the path from the weighing valve 7 to the reaction tube 18 can be cleaned. Furthermore, if the wash water from the pipe 20' is discharged in the direction 1-1 to the drain pipe 16' connected to the switching valve 16, the pump 14, the valve 16, etc. can be washed.

Now, the reaction tube 18 into which the sample has been introduced together with the first reagent is moved by an arbitrary movement mechanism (not shown) such as a rotational movement mechanism, and at the position 18', the second reagent pump 21 moves the reaction tube 18 to the second reagent tank. A second reagent is introduced at 22. A part of the piping between the second reagent pump 21 and the reaction tube is placed in the preheating tank 15. The second. While the reagents are introduced, the mixture is stirred, although this will not be explained in detail.
Reactions of multiple types of liquids are promoted, and colorimetric measurements of the reaction process or the mixed liquid after the reaction are performed.

Problems in the reagent supply of the conventional apparatus as described above are as follows.

(1) The suspension of the first reagent sent to the reaction tube together with the sample is determined by the accuracy of the reagent pump 14, but a high-precision pump is expensive, and even an expensive pump has a limit to its accuracy, and the measurement The amount of reagents cannot be precisely controlled which affects accuracy.

(2) Since the reagent is supplied to the reaction tube via the reagent pump 14, it is necessary to thoroughly clean the inside of the pump each time a different first reagent is switched, and the pump must be expensive and chemically resistant. It is necessary to use

(3) Since the configuration is such that reagents are supplied via the reagent pump 14 and the switching valve 13, even after thorough cleaning, reagents may remain inside the pump 14 and the switching valve 13, or Precipitation of crystals has been observed, which is a major cause of cross-contamination.

(/I) In order to perform a stable reaction, it is necessary to heat the reagent sent to the reaction tube, and therefore a preheating tank is required.

(5) There are a large number of valves, and furthermore, there are many pipes connecting the six valves and each unit, making the device long and complicated, making the device expensive.

The present invention has been made in view of the above-mentioned fish, and provides an automatic chemical analyzer that can supply an accurate amount of reagent, can prevent single-stage contamination, and has a simple configuration.

The automatic chemical analyzer according to the present invention includes a movable member disposed between a first and second fixed member, a through hole for weighing a reagent formed in the movable member, and a reagent measuring through hole formed in the movable member so as to be connectable to the through hole for weighing. 1st and 2nd
a through hole for reagent introduction provided in each of the fixing members;
a reagent suction pump connected to the reagent introduction hole of the second fixing member; a reagent tank connected to the reagent introduction hole of the second fixing member; and a reagent tank connected to the reagent introduction hole of the second fixing member; a gas supply means connected to the reagent introduction hole of one of the fixing members; and a gas supply means connected to the reagent introduction hole of the fixing member. Introducing the reagent into the weighing hole of the movable member using the reagent suction pump,
By moving the movable member, the through hole is connected to the reagent leading through hole of the fixed member, and a weighed reagent reaction tube is transferred into the through hole by pressure gas from the pressure gas supply means. It is configured to derive.

An embodiment of the present invention will be described in detail below with reference to the accompanying drawings.

In the flow path system diagram shown in FIG. 2, the same parts as in FIG. 1 are given the same numbers, and detailed explanation thereof will be omitted. In the diagram, 3
Reference numeral 1 designates a first reagent balance valve consisting of first and second fixed members 32 and 33 and a rotating member 34, and the rotating member 34 has at least two through holes 34a and 34 having equal volumes.
．． B is pierced. A through hole corresponding to the through holes 34a and 34b is bored in each of the first and second fixing members, and one of the through holes 32a of the fixing member 32 is connected to a reagent suction pump 35, One through hole 33a of the fixing member 33 is connected to the one-way multi-way switching valve 16 for reagent selection.

The through hole 32a is branched in the middle, and the branched through hole 32c is connected to a cleaning liquid tank (not shown) via a pipe 36, and the cleaning liquid from the cleaning liquid tank can be optionally transferred to the switching valve 16. It can be flowed toward the connected pipe 16', and the 9-8-1 reagent weighing valve 31, the switching valve 16, etc. can be cleaned. The other through hole 32b of the first fixing member 32 is connected to the through hole 5 of the second fixing member of the sample weighing valve 7, and the other through hole 33b of the second fixing member 33 is connected to the switching valve 37. It is connected to the.

The switching valve 37 switches between the pressurized air from the compressor 38 and the heated and pressurized washing water from the washing water tank 39, and connects the through hole 33 formed in the fixing member of the reagent pressing valve 31.
supply to b. 40 is a 20th drug supply device, and this device 40 includes a first reagent scale opening valve 31. Suction pump 35. Switching valve 37. Valves, switching valves, pumps, etc. similar to compressor 38 are included to supply the second reagent to reaction tube 18 when it is moved to position 18'.

The sample weighing valve, the first reagent weighing valve 31°, the second reagent weighing station valve, the reaction tube 18, etc. are arranged in a constant temperature bath 41 whose temperature is maintained at, for example, 37°C.

Now, in the above-described configuration, a sample is introduced into the through hole 6a of the sample weighing valve 7 in the same manner as explained in FIG. be moved to. Here, when an arbitrary first reagent is selected by the switching valve 16 and the first reagent suction pump 35 is operated, the through hole 3/1. The selected first reagent is introduced into a. After the reagent is introduced into the through hole 34a, the rotating member 34 is rotated to a position where the through hole 34a is connected to the through holes 32b and 33b. After that, the switching valve 3
7 is switched from an intermediate position where it is not connected to anything as shown by the solid line, air pressurized by the compressor 38 is supplied to the valve 31, and the pressurized air causes the through hole 34. The first reagent weighed in a and the sample liquid weighed in the through hole 6a are supplied to the reaction tube 18. the sample and the first
For example, the reaction tube 18 supplied with the reagent is moved to the position 18' by a rotational movement mechanism, and at this position the second reaction tube 18 is
A second reagent is supplied from the reagent supply device. Thereafter, a colorimetric measurement of the mixed solution of the sample and each reagent is performed, but this measurement part is not shown in Figure 1. After the measurement is completed, the mixed liquid in the reaction tube is appropriately discharged, and the reaction tube 18 is again connected to the through hole 4b of the fixing member of the test 1 weighing valve.
7 is switched by the dotted line. Therefore, the cleaning water flows from the cleaning water tank 39 to the switching valve 37. First reagent weighing valve 31. The sample is supplied to the reaction tube 18 via the weighing valve 7, and each part is washed. After the cleaning, the switching valve 37 is switched as shown by the solid line, and pressurized air is again supplied to each valve and reaction tube to dry each part, so that each part is brought to an initial state in which other samples can be analyzed.

As described above, in the embodiment described above, since the reagent is accurately weighed using the weighing valve and mixed in the sample, highly accurate reaction measurements can be performed. or,
The trial volume is 371. Since it is precisely determined by the volumes of a and 34b, there is no need to use a highly accurate and expensive reagent suction pump 35. Furthermore, even if the reagent pump is contaminated with reagent, the reagent sent to the reaction tube does not pass through the pump, so there is no need to specially clean the pump, and there is no need for a chemically resistant pump. There is no risk of cross-contamination even when using aged reagents. Furthermore, the sample weighing valve 7
, the reagent switching valve 31, etc. are arranged in the thermostatic chamber, and while the sample introduced into the through hole 6a is moved to the position of the through hole 6b, the test Fl temperature is made equal to the constant temperature chamber temperature, and Through hole 34. The first reagent introduced into the through hole 34b
Since the temperature of the first reagent is made equal to the temperature of the constant temperature bath while the first reagent is being moved to the position, the preheating bath conventionally used can be made unnecessary.

FIG. 3 shows the main parts of an embodiment of the present invention, and the same parts as in FIG. 2 are given the same numbers. In this embodiment, instead of the sample weighing valve 7 and the reagent weighing valve 31 in the embodiment shown in FIG.
1.52.2 disposed in close contact between 53 and the fixing member.
A weighing valve 5G consisting of two rotating members 54.55 is provided. The first rotating member 54 is provided with two through holes 54a and 54b for sample weighing, and the first and second fixed members 51 and 52 are provided with through holes for sample introduction connected to the through hole 54a. 51a, 52a are drilled 12- The through hole 51a is connected to the sampling pump 56, and the through hole 52a is connected to the pipette 2 for sucking up the sample r1. The sample is sucked up by the pump 56 and introduced into the through hole 54a.

It should be noted that the through hole 51a has a part on its way or a movable member 54.
A branch pipe 510 is provided at the contact portion with the branch pipe 51c, and the branch pipe 51c is connected to a washing water tank (not shown). As a result, by flowing the cleaning water through the branch pipe 51c, the weighing hole 54a, the sampling pipette 2, etc. are cleaned. The second rotating member 55 has through holes 55a and 55 for measuring reagents.
b is bored, and the second and third fixing members 52, 53
The through hole 55ai for measuring the reagent and the connecting through holes 7t52c and 53a for introducing the reagent are bored in the through hole 55ai. The reagent introduction hole 52c is connected to a reagent pump 57, and the reagent introduction hole 53a is connected to a reagent selection valve 16 as shown in FIG. Therefore, when the reagent is sucked up by the reagent pump 57, the reagent is introduced into the reagent measuring through hole 55a. Part 1. The second and third fixing members are provided with through holes 51b, 52b, and 53b for leading out the weighed liquid, which communicate with the through hole 5'4b for measuring the sample and the through hole 55b for measuring the reagent.

The liquid lead-out hole 5L-)b is connected to the kombu 1 nozzer 38 or three washing water tanks via the cut-1 valve 37, and the liquid lead-out hole 51b is connected to the reaction tube 18. ing.

In the above-described configuration, after a sample is introduced into the sample weighing hole 54a and a reagent is introduced into the sample weighing hole 55a, the rotating members 54 and 55 are rotated,
The through hole 5/la is moved to the position of the through hole 54b, and the through hole 55a is further moved to the position of the through hole 5'5b. If pressurized air is supplied from the compressor 38 in this state, the weighed sample 1 and reagent are transferred to the reaction tube 18. This embodiment achieves the same effect as the embodiment shown in FIG.
Compared to the embodiment shown in Fig. 2, which uses multiple valves, piping, etc. to connect each valve is unnecessary, the flow path can be shortened, the device can be purified to IIi, and it can be made compact. It can be done. Furthermore, since the unit is reduced to 1/2, for example, it is possible to go down from 1 to 1, and the failure rate is also reduced.

Although the present invention has been described in detail above, the present invention is not limited to the embodiments described above and can be modified in many ways. for example,
In the embodiment shown in Fig. 2, sample Y) was collected using a pipette and sample r1 was weighed using the valve cut method, but this collection and weighing may also be performed using Haku's method. . In addition, in the embodiment shown in FIG. 3, the weighing valve 56 is arranged in close contact with four fixed members and between these fixed members.
Instead of the valve, the second reagent may be weighed and supplied by the third and fourth fixed members of the third rotary unit. Furthermore, the number of weighing holes drilled in each rotating member is 2.
The structure is not limited to three, but may be three or more, and the volumes can be made to be different, and the amount of sample or reagent can be arbitrarily changed by selecting the type of through-hole. It is desirable to do so.

15-

[Brief explanation of drawings]

FIG. 1 is a flow path system diagram showing a conventional automatic chemical analyzer, FIG. 2 is a flow path system diagram of an automatic chemical analyzer using a reagent supply device based on the present invention, and FIG. 3 is a flow path system diagram showing a conventional automatic chemical analyzer. FIG. 3 is a flow path system diagram showing an example. 1: Try-on 1 tube 2: Sampling pipette 4.5.32, 33: Fixed member 6.34: Rotating member 7: Sample weighing valve 9: Sampling pump 12: Washing water tank 17: First reagent tank 18: Reaction tube 31 :Reagent weighing valve 35:Reagent pump 38:Compressor 39:Washing water tank 16-/'7(Ll'lb)'t. Figure 3

Claims (1)

[Claims] 1. A movable member disposed between a first and a second fixed member;
A through hole for measuring a reagent formed in the movable member, a through hole for introducing a reagent provided in each of the first and second fixing members so as to be connectable to the through hole for measuring, and a through hole for introducing a reagent in each of the first and second fixing members. A reagent suction pump connected to the reagent introduction hole, a reagent tank connected to the reagent introduction hole of the second fixing member, and the first and second fixing members connectable to the weighing hole. a reagent lead-out hole provided in each of the fixed members, and a gas supply means connected to the reagent lead-out hole of the one fixed member, and a movable member connected to the reagent lead-out hole of the fixed member A reagent is introduced into the weighing hole by the reagent suction pump, and the moving member is moved to connect the weighing hole to the reagent outlet hole of the fixed member, and the pressure gas from the pressure gas supply means is used to introduce the reagent into the weighing hole. A reagent supply device for an automatic chemical analyzer, etc., configured to lead out a reagent weighed into the through hole into a reaction tube. 2. a first moving member disposed between the first and second fixed members; a second moving member disposed between the second fixed member and the third fixed member; A sample weighing hole bored in the first moving member, the first and second sample weighing holes being connectable to the weighing hole.
A sample introduction hole provided in each of the fixing members, a sample suction pump connected to the sample introduction hole in one of the fixing members, and a sample suction pump connected to the sample introduction hole in the fixing member for use. A sample supply means, a reagent weighing hole drilled in the second moving member, and a reagent introduction hole provided in each of the second and third fixing members so as to be connectable to the reagent weighing hole. , a reagent suction pump connected to the reagent introduction hole of the one fixed member, a reagent tank connected to the reagent introduction hole of the fixed member for use,
the first, second, and third fixing members, each of which has a hole in the first, second, and third fixing members;
and a weighed liquid lead-out hole that can communicate through the sample weighing hole and the reagent weighing hole bored in each of the second moving member,
and a gas supply means connected to the lead-out through hole of the third fixed member, and sample weighing of the first movable member connected to the trial introduction through hole of the first and second fixed members. A sample is introduced into the reagent-weighing through-hole of the second movable member connected to the reagent-introducing through-hole of the second and third fixed members by the reagent suction pump. A reagent is introduced, and the two types of measuring through holes are connected to the deriving through holes of the first, second, and third fixed members by the movement of the first and second moving members, and the pressure saw gas is A reagent supply device for an automatic chemical analyzer, etc., configured to introduce a sample and a reagent weighed into the through hole into a reaction tube by means of pressure gas from a supply means. 3. The automatic reagent tank according to claims 1 and 2, wherein the reagent tank is arranged in multiple stages, and each reagent in the reagent tank is switched by a reagent switching mechanism and introduced into the reagent measuring through hole. Reagent supply device for chemical analysis equipment, etc. 4. A reagent supply device for an automatic chemical analyzer or the like according to claims 1 and 2, wherein the valve consisting of the fixed member and the moving member is placed in a constant temperature bath.