JPS5943357A - High speed liquid chromatograph - Google Patents
High speed liquid chromatographInfo
- Publication number
- JPS5943357A JPS5943357A JP57154197A JP15419782A JPS5943357A JP S5943357 A JPS5943357 A JP S5943357A JP 57154197 A JP57154197 A JP 57154197A JP 15419782 A JP15419782 A JP 15419782A JP S5943357 A JPS5943357 A JP S5943357A
- Authority
- JP
- Japan
- Prior art keywords
- detector
- gas
- flame
- section
- performance liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/84—Preparation of the fraction to be distributed
Abstract
Description
【発明の詳細な説明】
この発明は、高速液体クロマトグラフに関し、特にその
検出部の改良に関する。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a high performance liquid chromatograph, and particularly to improvements in its detection section.
高速液体クロマトグラフは、ガスクロマトグツ７が不得
意とするたとオげアミノ酸、特にシスディン、シスプー
ン、メチオニンなどのイオウ含有アミノ酸の分離分析の
ような分野の分析装置とＬ２て広く用いら）するばかり
でなく、最近はガスクロマトグラフが得意とする分野で
の分離分析にも使用されるようになってきつつある。High-performance liquid chromatographs are widely used as analytical devices in fields such as the separation and analysis of sulfur-containing amino acids, especially sulfur-containing amino acids such as cysdine, cyspoon, and methionine, which gas chromatographs are not good at. However, recently, gas chromatographs are also being used for separation analysis in fields where gas chromatographs are good at.
しかし、高速液体クロマトグラフの検出器たとえば紫外
線吸光光度計検出器、紫外線吸光光度計検出器、けい光
光度計検出器、示差屈折削検出器などは、臨床関係の検
出器として今ひとつ感度的に足りない点があり、゛また
選択性がない点で不便な場合がある。However, high-performance liquid chromatograph detectors such as ultraviolet absorption photometer detectors, ultraviolet absorption photometer detectors, fluorescence photometer detectors, and differential refraction detectors are not sensitive enough for clinical use. In some cases, it is inconvenient due to the lack of selectivity.
一方、気体分析に使用される検出器、たと乏げガスクロ
マトグラフに使用これる検出器、具体的には熱伝導度検
出器、水素炎イオン化検出器、炎光光度検出器、エレク
トロンキャプチャ検出器。On the other hand, there are detectors used in gas analysis, as well as detectors used in gas chromatographs, specifically thermal conductivity detectors, flame ionization detectors, flame photometric detectors, and electron capture detectors.
７１ノ一人サーミオニツク検出器乃どは、高感度で、強
い選択性をもつものもある。71 single thermionic detectors are highly sensitive and some have strong selectivity.
そこで、高速液体クロマトグラフの検出器として上舵の
ようなガスクロマトグラフに使用される検出器を用いる
ことができ１１げ、高感度の検出。Therefore, a detector used in a gas chromatograph, such as an upper rudder, can be used as a detector for a high-performance liquid chromatograph, resulting in highly sensitive detection.
Ｊ１択的−な検出を行うことができ、ガスクロマ・トゲ
ラフが不得意とする分野における分離分析は言う才でも
なく、ガスクロマトグラフが得意とする分野においても
高速液体クロマトグラフを広く利用でき、非常に便利で
ある。High performance liquid chromatography can be used for selective detection, and is not only good at separation analysis in fields where gas chromatography and togelaf are weak. It's convenient.
この発明は、このような事情に鑑みてなさｈたものであ
り、端的に述べれｒｊ゛、ガスクロマトグラフに用いら
れるような気体分析１１能な検出器を使用可能と（−た
高速液体クロマトグラフを提供するものである。This invention has been made in view of the above circumstances, and can be simply stated as: It is possible to use a detector capable of gas analysis such as that used in a gas chromatograph (-a high performance liquid chromatograph). This is what we provide.
以下、図に示す実施例に基いて、この発明を詳贋する。Hereinafter, this invention will be explained in detail based on the embodiments shown in the figures.
ただし、これによりこの発明が限定づ）上るものでｔ
ｉない。However, this does not limit the invention.
I don't.
第１図に示す（１）は、この発明の高速液体クロマトグ
ラフの一実施例であり、キャリア液送液ボング（２）、
試料導入部（３１９分離カラム（４）、紫外線吸光光度
計検出器（５）、気化器（６）９流路抵抗（ハお上びダ
ブル炎光光度検出器（８）が順に連設さハており、流路
抵抗（７）の後の流路にギヤリアガス吹込管（ＩｆＱが
接続されている。 （９）けギヤリア液槽である。(1) shown in FIG. 1 is an embodiment of the high performance liquid chromatograph of the present invention, in which a carrier liquid feeding bong (2),
Sample introduction part (319 separation column (4), ultraviolet absorption photometer detector (5), vaporizer (6), 9 flow path resistance (319), double flame photometer detector (8) are connected in sequence. A gear rear gas blowing pipe (IfQ) is connected to the flow path after the flow path resistance (7). (9) is a gear rear liquid tank.
気化器（６）の構造Ｖま、第２図に示すように、ギヤピ
ラリ−チューブｔｉｌｌの外因に加熱手段（＋２１を取
りｆ＝Ｊけたものである。As shown in FIG. 2, the structure of the carburetor (6) is such that the heating means (+21) is added to the external source of the gear pillar tube (f=J order).
流路抵抗（７）は、気化器（６）での気化時の流柘変動
を小さく抑乏、るため高抵抗にしである。The flow path resistance (7) is made high in order to suppress fluctuations in flow rate during vaporization in the vaporizer (6).
次に作動を説、＃４すると、試料が試料導入部（３）に
導入これた後、ギヤリア液がポンプ（２）によりたとえ
ば１０μ／′／分の流量で送液される。 試料μカラム
（４）で分離され、まず高速液体クロマトグラフ用検出
器である紫外線吸光光度計検出器（５）で測定される。Next, the operation will be described. At step #4, after the sample is introduced into the sample introduction section (3), the Gearia liquid is fed by the pump (2) at a flow rate of, for example, 10 μ/'/min. The sample is separated by the μ column (4) and first measured by an ultraviolet absorption photometer detector (5), which is a detector for high performance liquid chromatography.
紫外線吸光光度計検出器（５）を出グと後、カラム（
４）で分離きれた試料およびキャリア液ずなわぢ溶離液
（よ、気化器（６）中で加熱されて気化するが、流路抵
抗（７）があるので急激に噴出することはなく、たとえ
ば６〜Ｔｗｔ１分の流速で流路抵抗（ハを出る。 そし
て矢印（１（１のごとくギヤリアガスを加ズら］する。After exiting the UV spectrophotometer detector (5), the column (
The sample and carrier liquid separated in step 4) are heated and vaporized in the vaporizer (6), but because of the flow path resistance (7), they do not spout out suddenly, and e.g. Exit the flow path resistance (C) at a flow rate of 6 to Twt 1 minute. Then, increase the gear gas as shown by the arrow (1).
このギヤリアガス１ｆｔト乏げＩ（。This gear rear gas is missing 1 ft.
もしくはＮ、であり、流量１ｒＪｌ ｆｒ 、！ｌ：ｉ
ば２００＊ｆ／分である。 最後に、気化（−を溶離液
がギヤリアガスど共にガスクロマトグラフ用検出器であ
るダブル炎光光度検出器（８）に入るが、ギヤリア液槽
にσノ１−で検出にかからないので測定の妨害となるこ
とはない。 公知のように、ダブル灸光光度検出器は、
Ｓ、ｐｙＣ対１１．て選択性をイｊする。 そこで結局
、Ｓ、ｐに対し選択性が矛りがっＩＶ′脣へ度の検出器
を高速液体クロマトグラフに７５ｆたことになる。Or N, and the flow rate is 1rJl fr,! l:i
For example, 200*f/min. Finally, the vaporized (-) eluent enters the double flame photometric detector (8), which is a detector for gas chromatography, together with the gearier gas, but since it is not detected by σ in the gearier liquid tank, it may interfere with the measurement. As is known, the double moxibustion photometric detector is
S, pyC vs. 11. increase selectivity. Therefore, in the end, the selectivity for S and p was compromised, and the detector of 75 degrees to IV' was replaced with a high-performance liquid chromatograph.
ダブル炎光光度検出器（８）の出力Ｖ、」、紫外線吸光
光度計検出器（５）の出力ＶＣ幻して一定の遅Ｊ）時間
をもっているから、この遅ｉ１時間を考慮すＪｌば、両
出力を対応はぜることけ容易にできる。Since the output V of the double flame photometer detector (8) and the output VC of the ultraviolet absorption photometer detector (5) have a constant delay J), considering this delay i1 time, It is extremely easy to handle both outputs.
他の実施例としては、第３図に示すごとき気化器（６′
）を用いるものが挙けられる。 この気化器（６°）の
直前で矢印（１３のように溶自１「液にキャリアガスが
吹き込まれ、加熱ブロック（１４１内の空間（１４ａ）
にジェット流で噴出され、気化される。Another embodiment is a vaporizer (6') as shown in FIG.
). Immediately before this vaporizer (6°), a carrier gas is blown into the melt 1 liquid as shown by the arrow (13), and a space (14a) inside the heating block (141) is blown into the liquid.
It is ejected in a jet stream and vaporized.
捷た、紫外線吸光光度計検出器（５１１Ｃ代λて他のタ
イプの高速液体クロマトグラフ用検出器を用いたもの、
あるいはこれを省いてし捷つｆｒものが挙げられる。An ultraviolet absorption photometer detector (511C type λ and other types of high performance liquid chromatograph detectors used)
Alternatively, there are options that omit this.
さらに、ダブル炎光光度検出器（８）に代えて、他のタ
イプのガスクロマトグラフに用いられる検出器を用いた
ものが挙げられる。 たとえげフレームサーミオニツク
検出器全用いると、Ｎ、ｐｔｙｙ＋して選択的検出を行
いうるようになる。Furthermore, instead of the double flame photometric detector (8), a detector used in other types of gas chromatographs may be used. For example, if a full frame thermionic detector is used, it becomes possible to perform selective detection with N, ptyy+.
以上の説明から理解されるように、この発明Ｖま、キャ
リア液送液部、試ｔ１専入部、カラムおよび必要に応じ
高速液体クロマトグラフ用検出器をＩＩＦｊに連設し、
さらにその後に気化部、流路抵抗部および検出器をｊｐ
に連設し、かつ少なくとも流路抵抗部の後にキャリアガ
ス吹込管を接続【〜でなる高速液体クロマトグラフを提
供するものでおり、これによって従来より高感度の検出
あるいｔ、ｊ：選択的な検出を行いうるようになる。
また、非選択的な検出器を用いて液体クロマトグラフの
検出器との同時信号のクロマトグラフ・比較によって、
より冬くの情報をうることができ２・よ′）になる。。As can be understood from the above description, in this invention V, a carrier liquid feeding section, a section dedicated to test t1, a column, and a high-performance liquid chromatography detector if necessary are connected to IIFj,
Furthermore, after that, install the vaporization section, flow path resistance section, and detector.
The present invention provides a high-performance liquid chromatograph consisting of a carrier gas blowing tube connected to at least a flow path resistance section, which enables detection with higher sensitivity than before or selective detection of t, j: This makes it possible to perform various detections.
Additionally, by chromatographic comparison of simultaneous signals with a liquid chromatograph detector using a non-selective detector,
You will be able to get more information about winter. .
第１図はこの発明の高速液体クロマトグラフの＝１μＭ
［１例の描成Ｎ（を四回、第２図＆ｊ気化器の一楢成例
の｛芭成説明図、第３図シＪ．気化器の他の｛１Ｉ（成
ｆｌｊのｌｔＪｉ面図である。
（１）・・・高速液体りＶＺマトグラフ、（２１・ギャ
リア液送液ボンブ、（３１・一試料樽入部、（４）
カラム、（５）・紫外線吸′）Ｙ，光ＦＩ１−泊樟出器
、＋６１ （（ｉｏ）・一気化器、（７）・・・流路抵
抗、（８）・・・ダブル炎光光１ｉＪ″｛６丁出器、＋
１！’８ （＋６１・・−・Ｖヤリアガス吹込管。Figure 1 shows the high performance liquid chromatograph of this invention = 1 μM.
[Drawing one example of N (1) High-speed liquid VZ matograph, (21. Gallia liquid delivery bomb, (31. One sample barrel entry), (4)
Column, (5)・Ultraviolet absorber′)Y, optical FI1-night vaporizer, +61 ((io)・vaporizer, (7)...flow path resistance, (8)...double flame light 1iJ″ {6-piece dispenser, +
1! '8 (+61...-V Yaria gas blowing pipe.
Claims (1)
要に応じ高速液体クロマトグラフ用検出器を順に押設し
７、さらにその後に気化部、流路抵抗部ｊ、−よび検出
器を１）ｌｌ′ｌに押設［２、かつ少なくとも流、路抵
抗部の後にキャリアガス吹込管を接続し７たことを特徴
とする高速液体クロマトグラフ。 ２、流路抵抗部の後の検出器が、Ｓ−化合物およびＰ−
化合物を選択的に検出可能な炎光光度検出器である請求
の範囲第１項記載の茜速液体りｒ１マドグラフ。 ８、流路抵抗部の後の検出器が、Ｎ−化合物およびＪ〕
−化合物を選択的に検出器ｈ［２なフレームザーミオニ
ツク検出器である請求の範囲第１項記載の高速液体クロ
マトグラフ。[Scope of Claims] 1. A gear carrier liquid feeding section, a sample introduction section, a column, and if necessary a high-performance liquid chromatography detector are pushed in order 7, and then a vaporization section, a flow path resistance section j, - 1. A high-performance liquid chromatograph characterized in that: 1) a detector and a detector are 1) pushed to ll'l; 2. The detector after the flow path resistance section detects S-compound and P-
The Akanehoku liquid r1 magnetic graph according to claim 1, which is a flame photometric detector capable of selectively detecting compounds. 8. The detector after the flow path resistance section is the N-compound and J]
2. The high-performance liquid chromatograph according to claim 1, wherein the detector is a flame merionic detector that selectively detects compounds.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57154197A JPS5943357A (en) | 1982-09-03 | 1982-09-03 | High speed liquid chromatograph |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57154197A JPS5943357A (en) | 1982-09-03 | 1982-09-03 | High speed liquid chromatograph |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5943357A true JPS5943357A (en) | 1984-03-10 |
Family
ID=15578954
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP57154197A Pending JPS5943357A (en) | 1982-09-03 | 1982-09-03 | High speed liquid chromatograph |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5943357A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07306192A (en) * | 1994-05-12 | 1995-11-21 | T H I Syst Kk | Liquid chromatography for dispensation |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS56117166A (en) * | 1980-02-20 | 1981-09-14 | Hitachi Ltd | Detector |
-
1982
- 1982-09-03 JP JP57154197A patent/JPS5943357A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS56117166A (en) * | 1980-02-20 | 1981-09-14 | Hitachi Ltd | Detector |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07306192A (en) * | 1994-05-12 | 1995-11-21 | T H I Syst Kk | Liquid chromatography for dispensation |
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