JPS59223756A - Production of anthocyanin pigment - Google Patents

Abstract

PURPOSE:To produce the titled pigment in a high purity industrially advantageously, by purifying an aq. acidic extract contg. anthocyanin dye with a cationic or adsorptive resin and treating it with an ultrafiltration membrane. CONSTITUTION:A raw material contg. anthocyanin pigment, e.g. purple corn, red cabbage, berry of grape rind is extracted with an aq. acidic soln. having a pH of 4.0 or below. The extract is contacted with a cationic or adsorptive resin to adsorb the pigment on the resin. The resin is washed with water at 40 deg.C or below. The desorption of the pigment is then carried out with an aq. alcoholic or alkaline soln. at 40 deg.C or below. The pH of the resulting soln. is adjusted to 1.0-7.0 and the soln. is treated with an ultrafiltration membrane having 2,000-200,000 fractions. EFFECT:Foods which must have transparent appearance can be clearly colored without causing turbidity or precipitation.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to pigments and aims to obtain industrially advantageous highly pure anthocyanin pigments.

Anthocyanin pigments are found in purple corn, red cabbage,
It is well known that anthocyanin pigments are contained in grape skins, fruit juice, berries, and others, and it is a challenge for those skilled in the art to efficiently obtain high-quality anthocyanin pigments from these raw materials.

The present invention attempts to meet this problem by reducing the loss of the target dye in the low temperature range (40°C or less) and obtaining high quality. Let me explain benefit 1.

First, let us consider the water-soluble γ-forms of anthocyanin pigments.

Anthocyanin pigments are stably eluted from the above-mentioned 1 Kyomoto 1 with an acidic water extract. Examples of acids used in the acidic aqueous solution include hydrochloric acid, acid and other mineral acids, citric acid, tartaric acid, malic acid, and other organic acids.

pH is 4. Q or less is preferable because it improves the dye yield. I) When Ll exceeds 4.0 and the alkali level decreases to 1, the yield decreases.

The resin used to purify the aqueous solution containing anthocyanin pigments obtained from the extraction process is either cationic or adsorptive.

As the adsorption resin, Duoly I-X, A I) 2
, XAI)-4, XAI)-7, Diamond 1-1.
Examples include P-50, H, P-20 and others. As the cationic resin, Duolite Q-8, C-10, C-
20, S-30, and others can be opened. The purification method is as follows. The acidic aqueous solution containing the anthocyanin pigment is passed through the resin, and the pigment is adsorbed onto the resin. After washing with water (either the old flow or the reverse flow in 11), the resin is desorbed with alcohol/mineral water or alkaline water. In this step, it is important to keep the temperature of the armpit below 40°C.

At temperatures above 40°c2, the dye yield decreases and the hue becomes worse.

1) as a contaminant in this process! ! Quality, 'Uj (kind, fat, etc. are removed.About desorbed pigments, part 1)
■It is necessary to adjust 1. The pH reached is
The range is 1.0 to 7.0. I) If it exceeds l-1,7°0, the yield of the target product will decrease.

Next, the purified liquid is treated with an ultra-theta filter membrane. The type of membrane varies slightly depending on the raw material, but in general it is best to separate the molecular weight by fractionating the molecular weight (NIW) from 2,000 to 200,000, and the operation is carried out using the conventional method of limiting I = 200,000. good. For red cabbage extract, pressure 5 kq/e
yi, a flow rate of 15 n/min/- is optimal for teali and purple corn. This aqueous solution is also treated at a temperature of 40° C. or lower, as in the previous step. This process removes impurities such as ash, protein, polysaccharides, acids, and others.

The obtained anthocyanine dye is the desired high-grade anthocyanin dye.

According to the manufacturing process described in this article, anthocyanin pigments are of high quality and have a high yield, and when applied to various foods that require transparency, they are lip bright, and they do not cause turbidity, precipitation, or
Other problems have been resolved.

Here, the invention achieves its purpose.

In the resin-purified dye liquid, the permeation flow rate does not decrease when passing through the extreme p limit, and the dye and permeation are large when passing through the second limit.

Pigments that do not undergo ultrapolar permeation may cause precipitation over time when used in beverages, and if used in combination with other pigments or fruit juices, some may contain charged colloids and may form precipitates. No such phenomenon is observed in those that have been refined as stated above.

Example 1 1 part of purple corn seeds (weight, same hereinafter) was added to 1% (
weight, hereinafter the same) were immersed in an angular solution at 40°C for 30 hours to obtain a reddish-purple anthocyanin pigment extract (10'C).
1 In this example, the same applies hereinafter) was obtained. This solution was adsorbed onto 20 parts of Diaion J-L I'-50, washed with water, and the dye was eluted with 2 parts of 58 volume ethyl alcohol/I/1.

The dye solution obtained by distilling off the alcohol is first divided into a molecular weight cutoff of 6,
000 membrane was subjected to an ultraviolet filtration process using TUTO NTU 2006) to remove low-molecular impurities such as ash, bamboo, acid, and sugar. Next, after adjusting the pH to 3, Q with citric acid, low molecular weight impurities were removed. Next, a membrane with a molecular weight cutoff of 100,000 (2, !・
-NTIJ85100') to remove polymeric impurities such as protein, starch, and colloids.

These extreme lJE over-operations require a pressure of 5 kq/ca.
The flow rate was 111/tomo/-, and the permeation flow rate was 28.6 each.
It was 0 (at/pt//hr). Because of the pretreatment with plum oil, there was no clogging during ultrafiltration, a high permeation rate was obtained, and the degree of concentration was also very high.

The obtained pigment has a very bright red color and is suitable for acidic beverages,
When used for fruit juice drink*-1, etc., no scum formed over time.

Example 2 One part of cabbage was crushed, immersed in 1% citric acid ice water 2011 at 40°C for 30 hours, and then centrifuged and filtered to obtain a reddish-purple anthocyanin pigment solution. This dye solution was poured into 1 part of Duolite XAD-7, washed with water, and then eluted with 1 part of Δ58 V/V% ethyl alcohol. The dye solution from which the alcohol has been distilled off is divided into a molecular weight cutoff of 8,
000 membrane (2)1--NTU3508) sugar, ash,
Low molecular weight substances such as acids [excluding substances, then fractionated molecules - weight 10
Ultrafiltration was performed with a filter of 10,000 mm, 1 ψ, NTU 35100) to remove macromolecular colloidal impurities such as proteins, starches, and colloidal substances.

The obtained red pigment is used when coloring carbonated drinks and fruit juice drinks.
No occurrence of sludge over time was observed. Since red gabetsu contains positive colloid ions, if a method different from the conventional method is used, if negative colloid ions (tannin substances) are present in the fruit juice, they will co-precipitate over time. However, it was possible to color a fruit juice drink TFL-bright for the first time using the dye obtained by the method of the invention.

Example 3 After powdering ttil of dried elderberry for 17 days, it was soaked and stirred in 20 parts of 1% sulfuric acid at 40°C for 5 hours, and then the separated color liquid was adsorbed on 2 parts of Tire Ion HP-20 adsorption resin. After washing with water, it was eluted with 1 part of 58V/V% ethyl alcohol. Regarding the liquid from which the alcohol of the eluted dye was distilled off,
First, ultraf filtration was performed at a molecular weight cutoff of 5,000. Next, ultrafiltration was performed using a molecular weight cutoff of 10 ga.

These treatments remove low-molecular sulfuric acid, sugar, impurities and
゛Contains polymers such as tannin-like substances, proteins, and bleached powder! i:11
: Remove something. The obtained dye had a low viscosity even in a highly concentrated solution, and the aqueous solution was clear and little sludge formed over time.

Special f “Applicant Sanei Chemical Engineering Co., Ltd.

Claims (1)

[Claims] Acidic water extracts of purple corn, red cabbage, berries, grape skins, and other substances containing anthocyanin pigments are purified using a cationic or adsorbent resin, and further p I:L 7. After setting the value below Q, the ultrap-filtering film (
A method for producing an anthocyanin pigment characterized by treatment with fractions 2,000 to 200,000.