JPS59200633A - Multi-term simultaneous measuring apparatus using laminated elctrode cartridge - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a multi-item simultaneous measurement device using a laminated electrode cartridge.

Emergency testing requires rapid, accurate, and simple measurement of the ever-changing concentrations of various substances in living organisms. The actual measurement of these substances requires automatic analyzers that use various ion- or molecular-selective electrodes, such as glass membrane electrodes, solid membrane electrodes, liquid membrane electrodes, gas-permeable membrane electrodes, oxygen and hydrogen peroxide electrodes, etc. It is often used. However, in most cases, it can only be used after separating plasma or serum from a blood sample. Nothing has been seen so far.

On the other hand, substances in samples such as blood collected from living organisms include
There are substances that continue to change over time immediately after collection, and in order to measure these substances and use them as clinical information in emergency tests, it is necessary to complete the measurement immediately after collection or, if not yet, - air-cooled on ice. must be stored until measurements are taken.

Furthermore, there are some things, such as ionized calcium in plasma, where measured values change significantly due to changes in pH that occur with changes in sample carbon dioxide content immediately after blood collection. There are still problems with the device.

The present invention was made with the aim of improving the problems of conventional devices and methods as described above. When a specimen (for example, blood) is collected from a patient, some clinical information is collected from the specimen during the collection process. It is our goal to provide a multi-item simultaneous measurement device that is easy to operate and can perform various measurements quickly and accurately, and the collected sample can be used as a sample to obtain other information. There is.

According to the present invention, since blood flows directly into the container from the patient's blood vessels and the measurement is completed when the temperature, pH, etc. are still at approximately the same level as in the body, more reliable biological information is extracted. Moreover, the specimen can be used as a sample for transverse distortion of other biological information without being consumed at all in this process. However, the time it takes to retrieve information is significantly shorter, which significantly increases clinical usefulness.

Hereinafter, the present invention will be described in detail with reference to the drawings. ○ Figure 1 shows that the electrode electrolyte injection hole 1 of the laminated electrode cartridge according to the present invention is connected to the reference electrode plate electrolyte side of the measuring instrument main body, Each '15-pole enzyme membrane chamber for oxygen or hydrogen peroxide that constitutes the whole +Ii6. Sodium electrode 7 etc. are arranged, and they are connected to the potentiometer or ammeter of the measuring instrument body via terminals 8 and 9, and the calomel reference electrode (d
A porous plate 10 is provided, an enzyme identification membrane 11 is provided on the enzyme membrane electrode, and one of the sample holes 12 communicates with a blood collection needle connection part 13 and the other with a blood collection syringe connection part 14. 15 is a laminated electrode cartridge holder.

In addition, to explain the cross-sectional area of the apparatus of the present invention according to FIG.
7. The electrolyte chamber 18 degrees and the holder main body portion 19 are connected to the outer side of the electrolyte chamber 18 degrees, and electrode terminals and lead wires 20 are connected from the electrolyte chamber to the outer portion.

Regarding the operation of the device of the present invention configured as described above,
This will be explained with reference to FIG.

Mi) ※'Tun other pole cartridge (hereinafter referred to as cartridge) After holding the blood collection needle and syringe in the main body 21 and collecting the required amount of blood from the patient's vein, immediately hold the blood collection needle 22 horizontally. and separating the syringe 23 from the cartridge,
Immediately insert the cartridge into the measuring device main body 24.

(At Kashiwa, it is preferable to do this within 30 seconds after blood collection.) With this operation, the start switch (S) is pressed.
After that, use the in-machine automatic control system, electrolyte solution for the reference electrode (potassium chloride or potassium chain acid solution is used).
Approximately 3 μt of water is drained toward the main channel, thereby ensuring contact between the sample and the reference type +*2, and then reading and storing the potential difference of each electrode. Then, the standard solution B is
The blood in the cartridge is pushed out and injected to wash it away, and the potential generated at each electrode is captured again.The ion concentration of the sample is calculated by comparing it with the potential generated by the previous sample. The test in Figures 4 to 9: The 1st line is obtained by the above operation.The standard solutions A and C in the measuring instrument body, together with B, are installed in the cartridge at the start of daily measurement. The relationship between the potential difference and the ion concentration is stored in the calculation unit.
Perform electrode calibration or standardization. ) According to the present invention, quantitative analysis of plasma components can be performed at the same time as blood collection in an emergency or in general medical treatment without separating the plasma. Therefore, other biological components not incorporated in the cartridge of the present invention can be For the measurement, the blood collected and filtered in the syringe 23 can be transferred to a predetermined blood collection container, and the serum can be separated for measurement and testing. If all the measurement items for the target biological components to be tested are built into the cartridge, if blood fills the cartridge,
No further blood sampling is necessary.

Of course, each electrode of the laminated electrode cartridge of the present invention is an independent block and is designed to be able to be converted carefully. The membrane contains glucose oxidase, uricase, catalase, and hydrogen, depending on the test item to be measured.

Replaced with ruhinate oxidase, L-amino acid oxidase, etc., and pH, NH3, urea for the ion electrode.

L I + GL, Pb2″+ Af/ ++ F−
It can be used in place of the known ones.

As explained below, according to the present invention, the laminated electrode car 1
- When collecting a specimen (blood) from a patient by setting the IJ 7-ji type small and easy-to-use device between a blood sampling needle and a syringe, it can be incorporated into the device of the present invention during the process. With the stacked electrodes, clinical information can be extracted from the specimen, and the specimen collected in the syringe can be used as a sample to obtain information on other parts of the specimen, making it quick and accurate in emergency testing. , I can answer the question 11ji flight.

[Brief explanation of drawings]

Fig. 1 is a longitudinal cross-sectional view for explaining an embodiment of the device of the present invention, Fig. 2 is a cross-sectional view for explaining an embodiment of the device of the present invention, and Fig. 3 is a cross-sectional view of the device of the present invention. FIGS. 4 to 9 are schematic diagrams of the entire structure of the device for explaining the embodiments, and are calibration curves showing the potentials of biological components measured by the device of the present invention with respect to the intensities. 1... Electrolyte injection hole for reference electrode, 2... Reference electrode. 3.Chlorine electrode, 4..Q electrode, 5..K electrode. 6... Enzyme membrane electrode, 7... Wind electrode. 8.9...Terminal of each electrode 10...Porous plate. 11. Immobilized enzyme membrane, 12.16. Sample passage. 13. Blood sampling needle connection part, 14. Blood sampling needle connecting part. 15, 19... Holder, 17... Spacing fixing ring. 18... Electrolyte chamber, 20 - Lead wire. 21... Laminated "15-pole cartridge body, 22 Blood collection needle. 23 - Injection container, 24 - Measuring device main body control figure 3 - 11 peko; +4 convex 0.2 0.5 1 23 5 10f2'-Y"
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O8m Test technique 'f'4 豹宸ff (mg/di) ': A79 country 0 50100 200 300

Claims (1)

[Claims] Each electrode is a stacked electrode cartridge that is individually convertible, and is installed between a blood collection needle and a blood collection syringe. A multi-item simultaneous measurement device using a laminated electrode cartridge that measures components simultaneously.