JPS59199049A - Test tube for solid-liquid phase reaction - Google Patents

Abstract

PURPOSE:To perform separation of solid phase carrier from liquid phase easily by providing a stopper to the mouth side of a test tube so as to prevent discharge of the solid phase carrier contained in the test tube when the main body of the test tube is inclined or turned over. CONSTITUTION:A stopper 2 is provided to the mouth side 3 of a test tube in order that the solid phase carrier 7 contained in the test tube might not be discharged when the main body 1 of the test tube is inclined or turned over. With this test tube, separation of solid phase carrier 7 from liquid phase 8 (a liquid reagent for the reaction, and liquid sample or washing liquid for the solid phase) necessary for determining the substance to be tested in the liquid sample by catching the change in the solid phase after carrying out a solid-liquid reaction between the solid phase carrier, reagent liquid for the reaction and the liquid sample contg. the substance to be tested, is easily performed.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to an immobilized reactant (hereinafter referred to as a "solid phase carrier"), which is useful in a method for quantifying a test substance in a liquid sample, and a liquid phase (reaction reagent and liquid sample). The present invention relates to a solid-liquid phase application test tube for simplifying separation from a sample (or a solid phase carrier washing liquid).

In recent years, immunological methods that utilize antigen-antibody reactions and biochemical methods that utilize enzymatic reactions have been frequently used as clinical testing tools. In these methods, when separating or recovering reactants and unreacted products after the reaction, the operation is extremely complicated and time consuming.

Radioimmunoassay (RIA), developed by Berson and Yallow in 1959, is used to measure trace amounts of substances such as hormones and cancer antigens. In this method, a test substance, the same substance as the test substance labeled with a radioactive substance (hereinafter referred to as rRI-labeled substance), and an antibody against the test substance are reacted, and then the antibody-RI
The test substance is quantified by measuring the radioactivity of the labeled substance conjugate CB] or the radioactivity of the RI [[substance CFI] that is not bound to the antibody. At this time CB)
Separate and (F) (hereinafter r [B) / CF,) Separation”
), which is usually done by centrifugation.

However, in recent years, in order to simplify this separation procedure, antibodies are immobilized (insolubilized) on solid phase carriers, and CB) /
CF') methods that facilitate separation have come into frequent use.

In addition, enzyme immunoassay (EIA), which applies the principles of RIA and uses enzymes as a marker instead of radioactive substances, is 19
It was developed by Engvall and Perlmann in 1969. Generally, like RIA, EIA requires CB)/CF) separation, and the separation of [B] (J/[F] is facilitated by using a spherical, cylindrical, or gear-shaped solid phase carrier with antibodies immobilized on it. E IMB Le F]
After separation, the enzyme activity is measured, but since the enzyme activity is extremely sensitive, if the CB)/1 m F m1 separation is not completed completely, an error will occur in the quantitative value of the test substance. Therefore, in order to prevent such errors in quantitative values, in the method of measuring the enzyme activity of an enzyme labeling substance bound to a solid phase carrier, after separating the antigen-antibody and support, the solid phase carrier, and the reaction solution, the solid phase carrier is It is necessary to completely remove unreacted labeling substance CF by repeatedly washing the sample 3 to 5 times before starting the enzymatic reaction. At this time, the separation of the solid phase carrier and the reaction solution, and the separation of the solid phase carrier and the washing solution are performed using an aspirator.
A method using suction is often used, but this method requires a lot of time and effort. Therefore, as an alternative to the suction method, by using a reaction test tube whose inner wall is a solid phase carrier, after the reaction, the test tube is gently tilted to drain the reaction liquid, and after washing by adding a washing liquid, A method of discharging the cleaning solution in a similar manner has been put into practical use. Although this method simplified the washing operation, it was an inefficient and unproductive method because it required a step to immobilize antibodies on each tube at the stage of manufacturing the test tubes.
Problems also remained in terms of cost and accuracy.

The present invention was achieved as a result of studies aimed at solving the problems in the existing methods as described above.

That is, the present invention provides (1) a stopper 2 is provided on the mouth 3 side of the test tube 18 so that the solid phase carrier 7 contained inside the test tube is not discharged when the test tube main body 1 is tilted or overturned; (2) One end of the stopper 2 is integrally molded so as to be located at a part of the edge 4 of the opening 3 of the test tube body 1;
or the above-mentioned (1), wherein the stopper is fixed or rotatably attached to the position, and the other end of the stopper is removably fitted or attached to the edge portion on the opposite side from the position; This is a test tube. Furthermore, (3
) The test tube according to (1) above, wherein the stopper 2 is removably fitted or attached to the opening 3 side of the test tube body 1.

The solid-liquid phase application test tube of the present invention is equipped with a stopper on the test tube opening side to prevent the solid phase carrier placed inside the test tube from being discharged. , only the liquid phase inside the test tube can be discharged. Therefore, the solid-liquid phase application test tube of the present invention provides a method for separating solid and liquid phases with extremely superior operability compared to the conventional method of suctioning the liquid phase using an aspirator.

For example, when using an aspirator, it is necessary to insert a water absorption tube into each test tube, but when using the solid-liquid phase application test tube of the present invention, this is not necessary, and the test tube If you use something like a test tube stand that simply fixes multiple tubes at the same time, you can separate the same-phase carrier and liquid phase for many test tubes at the same time, so the separation operation is easier than using an aspirator. It has many advantages, such as the time and effort required can be greatly shortened and reduced, and additional equipment such as an aspirator is not required.

In addition, the solid-liquid phase application test tube of the present invention uses solid phase supports such as spheres, cylinders, gears, etc., which are commonly used at present. There is no need for inefficient or unproductive methods.

Next, the present invention will be explained with reference to the drawings.

FIG. 1 is an overall view showing an example of a solid-liquid phase application test tube of the present invention. In Fig. 1, l is the test tube body, and 2 is a stopper.The test tube body and stopper bar are integrally molded, or the stopper is fixed to the test tube body, or the stopper is attached so that it can rotate freely. It is formed by In FIG. 1, 3 indicates the opening of the test tube body of the present invention,
4 indicates the edge of the opening.

FIG. 2 shows a state in which the solid phase carrier and the liquid phase are separated by placing the solid phase in the solid-liquid phase application test tube of the present invention, and only the solid phase carrier remains. The solid-liquid phase application test tube of the present invention can separate a solid phase and a liquid phase by extremely simple operations such as tilting or overturning. Also the fourth
As shown in the figure, if multiple solid-liquid phase application test tubes of the present invention are connected to 1o in a test tube chamber where multiple tubes can be easily fixed at the same time, the same phase and liquid phase can be separated simultaneously for multiple tubes. can do.

FIG. 5 is an explanatory diagram showing an example of the method of inserting the stopper 2 in the test tube of the present invention. If configured like this,
After placing the solid support in the test tube, it can be set very easily by simply pressing the stopper lightly with a fingertip or the like. Furthermore, the solid-liquid phase application test tube of the present invention can be constructed by fitting a stopper into a ready-made test tube 13 as shown in FIGS. 6 and 7. FIGS. 8 and 9 show other methods of setting the stopper, and numerals 5 and 6 in the figures respectively indicate a protrusion and four parts for fitting or mounting one end of the stopper.

In addition, as shown in FIG. 10, the test tube body 1 and the stopper 2 are
The test tube body may be configured separately, and the test tube body may be provided with a protrusion or a recess so that a stopper can be attached, and the stopper may be attached as necessary. Note that the shape of the protrusion or the four parts may be any shape as long as it is configured to allow the stopper to be set, and is not limited by the drawings.

FIG. 11 is an explanatory view showing an example in which one end of the stopper 2 is rotatably attached to a part of the edge 4 on the opening 3 side of the test tube body 1.

FIGS. 12 and 13 are plan views showing an example of the shape of the stopper in the test tube of the present invention. The shape of the stopper is not limited by this figure; in short, it may be configured so that it does not interfere with the discharge of the liquid phase in the test tube and leaves only the solid phase carrier; for example, it may be mesh-shaped. There may be.

It is advantageous to provide a notch 9 between the test tube body 1 and the stopper 2 as shown in FIG. 14, as this makes it easier to bend the stopper when setting it.

Although the test tube of the present invention is shown as having a removable stopper for convenient loading and unloading of the solid phase carrier, in cases where it is not necessary to take out the solid phase carrier from inside the test tube,
The structure may be such that the solid phase carrier is fixed in advance inside the test tube with a stopper set therein.

The material for the test tube of the present invention is thermoplastic resin.

A thermosetting resin or the like is used. As a thermoplastic resin,
For example, polyethylene, polypropylene.

Polymethyl methacrylate, polyvinyl chloride, polyethylene terephthalate, polybutylene terephthalate, styrene-acrylonitrile copolymer styrene-maleic anhydride copolymer, styrene-acrylic acid copolymer, styrene-methyl methacrylate copolymer, styrene- Butadiene co-fi combined.

Styrene-isoprene copolymer, ethylene-propylene copolymer, ethylene-acrylic acid copolymer.

Ethylene-acrylic acid ester copolymer, polyvinyl alcohol acetal, polyvinyl alcohol butyral, etc., and as the thermosetting resin, for example, unsaturated polyester resin, epoxy resin, epoxy acrylate resin, etc. are used.

[Brief explanation of the drawing]

FIG. 1 is an overall view showing an example of a solid-liquid phase application test tube of the present invention. FIG. 2 shows a state in which a solid phase support and a liquid phase are placed in the solid-liquid phase application test tube of the present invention, and a stopper is set. FIG. 3 shows a state in which only the solid phase carrier remains after the liquid phase portion is separated by tilting or overturning the test tube in the state shown in FIG. FIG. 4 shows a state in which a plurality of solid-liquid phase application test tubes of the present invention are fixed in a test tube chamber where they can be easily fixed. 5 to 10 are explanatory diagrams showing a method of setting a stopper in the solid-liquid phase application test tube of the present invention. FIG. 11 is an explanatory diagram showing an example of a method for rotatably mounting one end of the stopper. 12 and 13 are plan views showing an example of the shape of the stopper in the solid-liquid phase application test tube of the present invention. FIG. 14 is a sectional view taken along the line AA' in FIG. FIGS. 15 and 16 are general views showing other embodiments of the solid-liquid phase application test tube of the present invention. 1. Test tube body 2. Stopper 3. Opening 4
, Edge 5. Projection 6. Recess 7, solid support
8. Liquid phase 9. Notch 10, test tube room
11. Hole 12. Spout■3. Applicant Chugai Pharmaceutical Co., Ltd. “6 figure sai’fu(ζ 小・9wazu(・g1suo□t(1knee)”:h

Claims (4)

[Claims] (1) A stopper 2 is provided on the test tube opening 3 side to prevent the solid phase carrier 7 placed inside the test tube from being ejected when the test tube body 1 is tilted or overturned. Characteristic solid-liquid phase application test tube. (2) One end of the stopper 2 is connected to the opening 3 of the test tube body 1.
The stopper is integrally molded or fixed or rotatably mounted at a part of the edge 4 of the stopper, and the other end of the stopper is located at a part of the edge 4 on the opposite side from the position. The test tube according to claim 1, which is adapted to be removably fitted or attached. (3) The test tube according to claim 1, wherein the stopper 2 is removably fitted or attached to the opening 3 side of the test tube body 1. (4) The test tube according to claim 2 or 3, wherein the stopper 2 is provided with a protrusion 5 or a fourth part 6 so that the stopper 2 can be fitted or attached to the edge 4 portion.