JPS59170015A - Treatment of animal plasma - Google Patents

Abstract

PURPOSE:To obtain globulin and albumin useful a raw material for foods, feeds, drugs, etc. simply and economically, by adding a Ca solution to animal plasma so that it is adjusted to a specific pH, fractionating precipitated fibrinogen effectively. CONSTITUTION:A plasma fraction is collected from blood of animal such as swine, bovine, etc. by a common separation method, an aqueous solution of a Ca salt or calcium hydroxide is added to the plasma, and the solution is adjusted to 6-10 pH, preferably 7-9 pH, so that precipitated fibrinogen is agglomerated and fractionated effectively, to collect plasma protein of globulin and albumin. An amount of the Ca solution added is properly 0.02-0.08 molar concentration based on plasma. This method is different from conventional ammonium sulfate method, sodium sulfate method, ethanol fractionation method, heavy metal salt method, etc., and it is simple and economically advantageous.

Description

Detailed Description of the Invention The present invention effectively eliminates fibrinogen, which plays a central role in blood coagulation, when obtaining major plasma proteins such as globulin and albumin from plasma obtained by centrifugation or membrane separation of animal blood. This invention relates to a method for agglomeration separation.

In Japan, a small portion of animal blood is used as plasma fraction for animal medicine and food, or as dried blood meal for fertilizer and feed materials, but the majority is not used effectively. This is the current situation.

The present inventors have conducted extensive research into methods for obtaining plasma proteins, which are expected to have a wide range of uses, from such unused blood, particularly proteins with high globulin and albumin content. We have discovered a simple and economically advantageous method that is different from the ammonium sulfate method, sodium sulfate method, ethanol fractional precipitation method, phosphate method, and heavy metal salt method.

That is, after adding sodium citrate as an agent to prevent gelation of fibrinogen to the blood collected internally from the carcass of an animal such as a pig or cow, plasma is separated by a conventional centrifugation method or a separation method using a plasma separation membrane. Collect the fractions, mix with calcium salt or calcium hydroxide water bath liquid gold,
By adjusting the pH of the solution to a range of 6 to 10, preferably 7 to 9, and separating the precipitated fibrinogen, all plasma proteins such as globulin and albumin, which have high utility value, can be easily collected.

The advantage of this method of collecting plasma proteins such as globulin and albumin is that, unlike the conventional salting-out method, it does not require a large amount of ammonium sulfate or sodium sulfate, and it does not have problems such as large amounts of inorganic salts contaminating the fractionated proteins. , like the alcohol precipitation method,
There are no problems with solvent recovery, and no toxic heavy metals are used.

To describe the method of the present invention in more detail, blood is collected from an animal carcass in a trench, and the entire calcium solution is added to the plasma fraction from which blood cell components have been removed by a conventional method. 0.01-D, 1 mol (I
IJ() range, particularly 0.02 to 0.088 molar concentration, is suitable; outside the above range, a kelization phenomenon occurs, which is undesirable.
``To get out, set 17H at night to 6-10, preferably 7.
It is important to keep the temperature within the range of 9 to 9, and if you deviate from the above range, you will not be able to achieve the full purpose. In addition, water can be added to the plasma as needed to facilitate the precipitation of fibrinogen and its removal from solution. The drawn fibrinokene fraction is usually f-■
Fractions containing white matter such as globulin and albumin, which can be easily separated by conventional filtration or centrifugation, can be desalted and purified as necessary to produce food, nitrogen, etc. On the other hand, fibrinogen, which can be compressed into one minute, has new uses such as food additives and medical products.
Waiting.

Next, the present invention will be explained in detail with reference to Examples.

Example 1 Quickly add 1 scoop of fresh blood obtained from the adult body layer in a wet field.
After adding 30me of 0% sodium citrate bath solution, centrifugation was performed under water cooling, plasma, i! :) I gained 0.55 l. To 500 ml of this plasma, add 10-fiber chloride (
dihydrate salt) dissolved JW 30 mb (J water 15 G mlf
Added (calcium salt id to plasma approximately o,
Fibrinogen was completely precipitated by stirring at room temperature for about 6 minutes at a pH of 8.2 (total pH 8.2). Next, this was separated by kP, and a fibrinokun fraction of 47.59 mm (water content approximately 90%) and a globulin/albumin fraction of approximately 650 m1f were collected. Nao, the content ratio of plasma protein globulin and albumin in the latter is
As a result of measurement by gel electrophoresis, it was approximately 7:10,
Almost no fibrinogen was included.

Example 2 Quickly add 10% to approximately 2 tons of fresh blood collected from a cow in Laiyang
After adding all 50 ml of sodium citrate solution, cool with water,
By centrifugation, the plasma fraction of the disupernatant was separated by approximately 1. I got o5tk.

Collect 1 t of plasma, add 1 t of water and 25 ml of 5% lucium hydroxide solution, adjust the pH to 8.8 with hydrochloric acid, stir, and leave overnight at 4-8°C. The precipitated fibrinogen was thoroughly separated, and about 1.9 g of the protein fraction bath solution containing all of the transparent globulin and albumin was swallowed. Such protein fraction was
When analyzed by the #J method, almost no fibrinokun was detected.

Claims (1)

[Claims] An aqueous solution of calcium salt or calcium hydroxide is added to plasma obtained from animal blood, and the r)H'k of the solution is
A method for treating animal plasma in which the precipitated fibrinokene is fractionated and all plasma proteins such as globulin and albumin are obtained by adjusting the concentration within the range of 6 to 10.