JPS59169491A - Stabilization of enzyme - Google Patents

Abstract

PURPOSE:An enzyme is coated with a phospholipid to give the enzyme resistance to oxidation whereby the enzyme is not deactivated, even in combination with a peroxide. CONSTITUTION:A phospholipid such as lecithin (phosphatidyl choline) or cephalin (phosphatidyl serine) is dissolved in an alcohol solvent such as ethanol or water/ ethanol. The resultant solution is mixed with an enzyme such as protease, lipase or amylase in a rotary mill, then dried to effect coating.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for stabilizing an enzyme, and more particularly, to a method for stabilizing an enzyme characterized by coating the enzyme with a phospholipid.

Conventionally, enzymes such as grotease, amylase, and lipase have been widely used in the field of detergents and the like because they are effective in removing stains such as proteins, starches, and fats. However, these enzymes have the function of acting on macromolecular contaminants such as proteins [2] and reducing their molecular weight to increase their solubility in water. Since it is impossible to completely decompose it, it is used in combination with sodium percarbonate, sodium perborate, etc.

However, when these enzymes are used in combination with the above-mentioned oxidative bleaching substances, they have the disadvantage that the enzymes themselves are oxidized during storage or use, making it impossible to obtain the desired enzyme activity.

That is, the present invention imparts oxidative stability to the enzyme by coating the enzyme with phospholipid, and the enzyme activity is not lost even when used in combination with a peroxide compound such as sodium percarbonate. It has the characteristic that there is no

The phospholipid used in the enzyme coating treatment in the present invention is one of complex lipids, and includes phosphatidic acid, phosphatidylglycerin, and calciolipin, which are composed of glycerin, fatty acids, and phosphoric acid; Phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, which have a nitrogen-containing atomic group as a constituent, plasmalogens which have one aldehyde instead of a fatty acid, and lysophospholipids, lysoplasmogens, which have only one fatty acid, etc., especially Lecithin (phosphatidylcoli/), heptanoline (phosphatidylserine), and the like can be suitably used.

Deaf phospholipids are generally easily oxidized and therefore act as antioxidants when used in enzyme coatings. For example, when this coated enzyme is mixed with a peroxide compound such as soda percarbonate, As a result of effectively blocking oxygen generated by the decomposition of soda, the effect of effectively preventing deactivation of enzyme activity is achieved.

In addition, this phospholipid is generally water-soluble or water-dispersible and has a surfactant effect itself.
When this coated enzyme is used in detergents, whitening agents, etc., not only does it not cause permanent retention, but rather the enzyme acts selectively on stains such as proteins and decomposes these stains. It has the advantage of improved cleanability.

Enzymes used in the present invention include protease,
Any enzyme can be used, such as lipase, amylase, etc.
Proteolytic enzymes, especially alkaline proteases, can be preferably used. In addition, this enzyme is produced not only by itself but also by lubricants such as polyethylene glycol, sodium chloride, sodium sulfate, pentasodium tripolyphosphate, tetrasodium birophosphate or the corresponding potassium salt, cellulose powder, starch powder, cellulose derivatives, and starch decomposition products. It may be mixed with or supported on fillers or binders such as starch derivatives, gelatin, casein, skim milk powder, polyvinyl alcohol, and polyvinylpyrrolidone.

In the coating treatment, the above-mentioned phospholipids are coated with ethanol or water/
This can be easily carried out by dissolving the enzyme in an alcoholic solvent such as ethanol, mixing it with the enzyme alone or with the enzyme supported on a commercially available carrier in a rotary mill, and then drying. Of course, the phospholipid solution may be treated with the above enzyme by spraying or the like.

In addition, during coating treatment, the ratio of enzyme and phospholipid is usually 100:0, although it varies depending on the type of enzyme and phospholipid used.
．． 5 to 100:100, especially 100:1 to 100:
It is desirable to mix them in an amount ratio of 50%. If the amount of phospholipid is less than the above range, the desired oxidative stability cannot be obtained,
For example, the enzyme activity is deactivated by mixing with a peroxide compound such as percarbonate. Further, even if the amount of phospholipid is greater than the above range, there is no particular advantage and it is economically disadvantageous.

The enzyme or carrier-retaining enzyme used in the coating treatment may itself be in the form of powder or granules; for example, enzyme pellets obtained by extruding a single enzyme or an oleaginous enzyme with a stabilizer such as polyvinylpyrrolidone together with a small amount of water may be used. It is easily soluble in hot water as well as cold water and can be suitably used.

The enzymes stabilized by the method of the present invention have excellent oxidative stability, and even when used in combination with peroxide compounds such as sodium percarbonate and sodium perborate, the enzymes stabilized during storage or use.
It is extremely useful in the fields of detergents, bleaches, etc., without losing its enzyme activity.

The invention is illustrated by the following example.

Sample preparation: Protin/Is (alkaline protease manufactured by Daiwa Kasei) is used as the enzyme, mixed with various coating agents, deionized water is added to this mixture, stirred, and then ethanol is added in the same amount as the deionized water. Then, this mixture was dried under reduced pressure to powder, and this powder was mixed with sodium percarbonate (5Na, t
CO, ``2H,O□, PC'' was mixed to prepare a sample powder.

The blending conditions for each sample powder are as shown in Table 1.

Table 1 Example 1 Each sample powder prepared as above was placed in a glass bottle,
It was stored in a constant temperature bath at 37C, and changes in enzyme activity over time were measured.

The results are shown in Table 2.3 The enzyme activity was measured using the casein-Folin method.

Table 2 From the results, it can be seen that in the enzyme coated with phospholipid according to the present invention, deactivation of the enzyme activity is effectively prevented even when mixed with sodium percarbonate. .

Example 2 Using each sample used in Example 1, a soaking test was carried out with a protein-contaminated cloth at each time of storage.The 6-contaminated cloth was EMPAl 16-protein-contaminated cloth (components: milk, blood, cinnamon, adhesion). After dissolving each sample powder (containing 22% of sodium percarbonate) in 20 ml of deionized water at room temperature, the contaminated cloth was immersed to determine the stain removal rate. Measurement 1. Ta.

The immersion temperature was 50 r, and the immersion time was 2 hours. 6th
The results are shown in the table.

Table 6 Patent Applicant Duskin Franchise Co., Ltd. Procedural Amendment (Voluntary) June 17, 1980 Director General of the Patent Office Mr. Kazuo Wakasugi1, Indication of Case Patent Application No. 58-415532, Name of Invention Enzyme Stabilization Law 3 Relationship with the Nagisa case to be amended Patent applicant 4, agent 〒105 5, No indication of order for amendment 6, Column 7 for detailed explanation of the invention in the specification to be amended, Contents of the amendment (1) On page 5, line 8 of the specification, the phrase "contains oil stabilizer" should be corrected to "contains stabilizer."

that's all

Claims (1)

[Claims] A method for stabilizing enzymes characterized by coating enzymes with phospholipids.