JPS59152316A - Dentifrice composition - Google Patents

Abstract

PURPOSE:A dentifrice composition obtained by using mutanase (a drug for preventing decay of the teeth) together with sorbitol so that mutanase is stably blended with the dentifrice composition containing aluminum hydroxide as an abrading agent. CONSTITUTION:Mutanase and sorbitol are blended with a dentifrice composition containing aluminum hydroxide as an abrading agent, to give a dentifrice composition keeping sufficient enzymatic activity of mutanase for a long period, showing improved preventing effect on decay of the teeth even if it is blended with aluminum hydroxide as the abrading agent. Preferably about 45-50wt% aluminum oxide is blended with 0.1-20wt% mutanase of 500-10,000units/g, 20-40wt%, preferably 30-40wt% sorbitol, and an N-acylsarcosine salt alone as a foaming agent or with a sucrose fatty acid ester based on total amount of the composition.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a dentifrice composition stably containing mutanase, which is a medicinal agent for preventing caries.

Prior Art Conventionally, it has been known that Streptococcus mutans, which is one of the most important bacteria for cavities, converts α-1,3-glucoside bonds from sucrose as one of the mechanisms of dental caries development.
It is said that mutan, a sticky glucan, is produced and firmly adheres to the tooth surface 1, where it produces lactic acid and demineralizes the tooth (Clinical and Bacteria, Vol. 1, p. 24). ,
(1974). Mutanase is an enzyme that decomposes the α-1,3-glucoside bond of mutan, and using this action,
Attempts have been made to prevent caries by disassembling Streptococcus mutans to prevent the attachment of Streptococcus mutans to the tooth surface (Shanal of Dental Research, Vol. 51, Suppl, p. 394) , 197
2 years). It has also been proposed to incorporate mutanase into toothpaste (Japanese Patent Publication No. 55-5006), and it has been recognized that mutanase is effective in preventing dental caries.

However, mutanase is extremely unstable, and when it is added to toothpaste, it tends to lose enzyme activity due to the influence of other ingredients. When using aluminum hydroxide as an abrasive, the stability of mutanase tends to deteriorate, and it is extremely difficult to stably incorporate mutanase into toothpastes that use aluminum hydroxide as an abrasive.

Purpose of the Invention In view of the above circumstances, the present inventors made extensive efforts to obtain a double-layer composition containing aluminum hydroxide as a till abrasive and a stable abrasive mutanase.
Surprisingly, it was discovered that sorbitol, which is commonly added to toothpaste as a humectant, specifically stabilizes mutanase, which is a double-layered abrasive acid containing aluminum hydroxide as an abrasive, and this led to the completion of the present invention. It happened.

The composition of the invention, that is, the double-layered acid product of the present invention is characterized by containing aluminum hydroxide, mutanase, and sorbitol as essential components. The point of non-invention is that (0) even if aluminum hydroxide is added as an abrasive, mutanase maintains sufficient enzymatic activity for a long period of time and exhibits excellent dental caries prevention effects. is obtained.

The aluminum hydroxide to be used may be normally blended into toothpaste as a TI:IF agent, and generally the blending deviation is about 45 to 50% (wt%, same hereinafter) based on the total amount of the composition.

Mutanase can be any commonly available one; for example, Trichoderma harzianum OMZ779, Talatosporium resinae QM7998, Streptomyces veerensis, Aspergillus nidorans, Flavobacterium nis bii, Pseudomonas sp. Kotoki mutanase-producing bacteria are cultured in a medium containing a normal nutrient source or in a medium to which α-1,3-glucan is added, and the supernatant is purified by salting out method, absorption method, solvent fractionation method, etc. Examples include those obtained by fractionation. The properties of mutanase vary slightly depending on the type of producing bacteria, but in general, the optimal pI-I of mutanase is on the acidic side, and it has the effect of decomposing mucun, liberating reducing sugars, and solubilizing them. In the present invention, the desired caries preventive effect can usually be obtained by blending 0.1 to 20% of mutanase in an amount of 500 to 100,000 units/g to the entire composition. In addition, mutanase 1
The unit is defined as the amount of enzyme that decomposes mutan at 40°C in 01M acetate buffer (pH 5, 7) and liberates 1 μM of reducing sugar in terms of glucose per minute.

Sorbitol may also be added to toothpaste, and is generally used in an amount of 20 to 40%, preferably 3% to the total amount of the composition.
Mix 0 to 40%.

In addition, a foaming agent is usually blended into toothpaste, but in the present invention, from the viewpoint of stabilizing mutanase, N-acylsarcosine salt is blended as the foaming agent alone or in combination with sucrose fatty acid ester. It is preferable.

[<-acyl→)-lucosine salt has the formula: [In the formula, II is an alkyl group and I4 means alkali gold or ammonium.] 11
~17 alkyl groups, preferably those of sodium or ammonium, such as N-lauroylsarcosine salt, N-myristoyl→norcosine salt,
N-balmitoyl sarcosine salt, f~-stearoyl→
Examples include norcosine salts etc.) 1', J-acylsarcosine salts account for 01 to 30% of all oxalates.
, preferably 0.1 to 0.5% self-association.

As a sucrose fatty acid ester, the number of carbon atoms in the fatty acid residue is 1.
0 to 1.8 higher fatty acids Fl and e', preferably consisting mainly of monoesters, for example, commercially available 1
Moon (ester F -50,, F -70,, F -'=
)0, F-i 10 , F-1II Ol)' -
160 (both manufactured by Dai-ichi Kogyo Seiyaku Co., Ltd., containing fatty acids (10 to 18 carbon atoms) from hardened beef tallow as fatty acid components, and the monoester content was 30.40.45.50.
60.70%), 1) I (Ester L-18 (manufactured by Daiichi Kogyo Seiyaku Co., Ltd., sucrose laurate ester) The toothpaste composition of the present invention is used in the usual manner when toothpaste is used. Other compounded ingredients include, for example, carpogysimethyl cellulose sodium, carakeenan, sodium polyacrylate, xanthan gum, and other binders, and H'' flavoring agents. Sodium fluorophosphate, chlorhexidine, anti-inflammatory agents and other medicinal agents may be added.

With the effect of the invention, aluminum hydroxide (U1 polishing mt
, ' shows the results of testing the stability of mutanase in '.

In accordance with the formulation shown in Table 1, aluminum hydroxide is used as an abrasive and various amounts of sorbitol are blended into the mucunase-containing wire layer (toothpaste of the present invention), and for comparison, sorbitol is replaced. Mutanase-containing toothpaste (comparative toothpaste 1), which contains chrycerin, a polyhydric alcohol similar to 2) was manufactured.

After storing the obtained toothpaste at 37°C for 30 days, the mutanase activity (amount of free reducing sugars according to the Somoky-Nelson method) in the toothpaste was measured, and the residual rate (%) was determined by comparing it with the activity immediately after production. I asked for it. The results are shown in Table 2.

Table 2 Table 2 does not show the number 2. In toothpaste containing glycerin, which is a polyhydric alcohol similar to sorbitol (comparison toothpaste), the residual rate of mutanase is high when the abrasive is calcium diphosphate. In the case of Kenreki All or aluminum hydroxide, the residual rate of mutanase is extremely low.

However, when sorbitol is added, even if the polishing agent is aluminum hydroxide, the residual rate of mutanase is high, and as the concentration of sorbitol increases, the residual rate improves, and sorbitol is a specific (mutanase) It is clear that stabilizing the

EXAMPLES Next, the present invention will be explained in more detail with reference to Examples.

Example 1 A toothpaste was manufactured according to the conventional method according to the following recipe.

Ingredients % Aluminum hydroxide 400 Solutriol 30.0 Sodium carboxymethyl cellulose 1. ON-Lauroylsarcosine sodium 0.5 Sucrose fatty acid ester (+1iW fatty acid ester from hardened beef tallow, monoester included @70%) 1.5 Saccharin sodium
0.2 fragrance
1.0 Mutanase (10,000 ri/9)
0.1 water
], 00% (Toothpaste was manufactured according to the conventional method according to the formulation with this adjustment M2.

% Aluminum hydroxide 45.0 Sorhitol 35.0 Carrageenan 1. ON-lawaloyl sarcosine sodium 05 Sucrose laurate 15 Zanocalin sodium
0.2 Kosai 11.0 Mutanase (5 blade position/11) 0.2
Water Adjust to 100%
I Applicant Zanstar Co., Ltd.

Claims (1)

[Claims] 0) IiJI' A toothbrushing composition containing aluminum hydroxide as a polishing agent (a toothbrushing composition characterized in that it contains mutanase and/or rubitol).