JPS591401A - Plant growth regulator - Google Patents

Abstract

PURPOSE:To provide the titled regulator exhibiting the activities of the growth promotion, rooting promotion and aging prevention of plants and freshness keeping for cut flowers, etc. at a low does, by using tetrahydroxyethyl-B-homooxacholestanone compound as an active component. CONSTITUTION:The objective agent contains 2alpha,3alpha,22S,23S-tetrahydroxy-24S- ethyl-B-homo-7-oxa-5alpha-cholestan-6-one of formula I or 3beta,22S,23S-trihydroxy- 24S-ethyl-B-homo-7-oxa-5alpha-cholestan-6-one of formula II as an active component. The above compound is mixed with various carriers and assistants, formed to an aqueous solution, liquid, emulsifiable concentrate, granules, etc. and applied to plants such as grains, green vegetables, flowers, etc. at a concentration of preferably 0.05-100ppm, especially about 0.1-10ppm.

Description

Detailed Description of the Invention 2α, 5α, 228,25B-tetrahydroxy-248-teruB-homo-7-oxa-5α represented by υ
-6β, 228, which can also be expressed by cholestan-6-one
, 238-) dihydroxy-24B-ethyl-B-homo-7-oxa-5α-cholestan-6-one as an active ingredient.

More specifically, the present invention relates to a plant growth promoter, a plant rooting promoter, and a plant antiaging/freshness preserving agent.

Traditionally, auxins have been used as plant growth regulators.

Gibberellin fields, cytokinins, etc. are known,
Gibberellins are actually effective plant growth promoters, and auxins such as IAA (indole acetic acid) and NAA (α-naphthalene acetic acid) are examples of rooting promoters. Cytokinins such as kinetin (6-furfurylaminopurine) and benzyladenine are known as anti-aging agents.

However, Gibberelli 7 has no practical effect as a rooting promoter or anti-aging agent (IAA and NAA have no practical effect as a growth promoter or anti-aging agent, and kinetin and benzyladenine e. It has no practical effect as a growth promoter or rooting promoter, and there is a strong demand in this field for the emergence of a drug that has practical effects that combine growth promoting, rooting promoting, and antiaging effects. This is it.

From the knowledge of the results of various basic tests regarding the present compounds represented by formulas (1) and (II) above, it has been found that they have actions different from those of conventional auxins, gibberellins, and cytokinins.

For example, the two compounds (1) have a lower activity d compared to indole-3-acetic acid (A A), which exhibits auxin activity.
Although it was more than 100 times stronger in the lamina joint test, it showed no activity in the general Avena test, which is a substance that exhibits auxin activity, and did not show parthenocarpic action as a known effect.

In addition, the induction of the ethylene biosynthetic system is weak, and from these facts it can be said that the properties of this compound are different from known auxin substances. Furthermore, gibberellin activity can be assayed by promoting elongation of lettuce hypocotyl, but these two compounds did not show this gibberellin activity.

However, cytokinin activity can be assayed by promoting the growth of radish cotyledons, and this compound showed this growth promotion.
However, unlike cytokinins, it did not have the strong ability to significantly promote auxin activity through its cooperative action with auxin in inducing the ethylene biosynthesis system.

From these results, it was found that this compound exhibits a physiological action different from that of conventional plant growth regulators. However, whether or not a drug has practical effects can only be confirmed by conducting various practical tests.

The present inventors further conducted practical tests and found that 9 of these compounds promoted plant growth and 1 promoted rooting.

We have completed the present invention by discovering that it is a drug that has practical effects that have both anti-aging and freshness-maintaining effects.

The action of gibberellin, a plant growth promoter, is primarily based on cell elongation, and its effect on increasing the dry weight of plants is small. (See Test Examples below), it was observed that not only did it promote elongation, it also promoted leaf development, and there was also a significant increase in fresh weight and dry weight.

Furthermore, when sprayed during the foliage stage of Kirari.

A significant increase in leaf area was also observed.

In other words, this compound can be said to have a practical effect as a plant growth promoter.

In the case of NAA, which has a practical effect as a rooting promoter, the effect of promoting rooting underground is reduced, but the effect of preventing aging of leaves above ground is small, but in the case of one compound, is NAA'//1 [1 (1) Even when treated with a low concentration of 1, it has a remarkable effect on promoting rooting, and also has a remarkable effect on preventing aging of above-ground leaves. It can be said that it is a drug with high practical value and effects.

Furthermore, when this compound was applied to crops such as rapeseed, its anti-aging effect was observed.

The effect of this compound as an anti-aging and freshness-preserving agent was also confirmed by the test result VC for improving the shelf life of cut flowers. In other words, compared to benzyladenine (cytokinin), which has a practical effect as a conventional anti-aging agent, '
Even when treated at a low concentration of /1oo, the freshness-preserving effect is excellent, and from this fact, the present compound can be said to be a drug with high practical value as an anti-aging and freshness-preserving agent.

As mentioned above, this compound can be used in a variety of situations.1 For example, by treating S bacteria with this compound at the time of transplantation, it is possible to maintain the vitality of the seedlings and enhance regeneration after transplantation. .

The compounds that are the active ingredients of the present invention are known.

For example, agricultural, cultural and biological
Chemistry (Agr+cultural and B
iological f'! h8Inistry) 4th
It can be synthesized by the method described in Vol. 5, p. 2579 (1981).

The plant growth regulator of the present invention can be used to promote plant growth as described above.
It has the practical effects of promoting rooting, preventing aging, and maintaining 1IIf degrees, and is 41! It can be applied to plants such as vegetables, flowers, etc.

The degree of use of this compound varies depending on the purpose of use, the type of plant to be used as a control, and other conditions;
00 ppm is desirable, more preferably [11-1
It is appropriate to use it at a degree of about 0 ppm.

When putting the plant growth regulator of the present invention into practical use.

Depending on the usage situation, it can be mixed with various carriers and made into an aqueous solvent.

It can be used as a liquid preparation, emulsion, granulation agent, etc.

Here, the neck carrier may be either solid or liquid,
A combination of these may also be used. Examples of these are Klee, Bentonite, and Merck.

These include diatomaceous earth, water, alcohol, benzene, acetone, etc., and additional adjuvants used in the formulation of agricultural chemicals, such as spreading agents, emulsifiers, surfactants, etc., may be used as appropriate.

Not only can each formulation be used alone, but
It may be mixed with other plant growth regulators or may be applied in combination with fertilizers.

Next, the present invention will be specifically described with reference to examples, but the present invention is not limited to these examples.

Example 1 Solution Compound (1) 11! 70% of ethyl alcohol for Iji part.
Dissolve in 1 part by weight and add 29 parts by weight of water to make a 1X solution. When using it, dilute it with 100 to 100,000 times more water to prepare a treatment solution.

Example 2 Milk agent 10 parts by weight of the present compound (,1), 70 parts by weight of xylene.

20 parts by weight of Tsurupol 2680 (a mixture of a nonionic surfactant and an anionic surfactant; manufactured by Toho Kagakuten Co., Ltd.) was mixed to prepare an emulsion. When using it, dilute it with 1,000 to 1,000,000 times more water to prepare a treatment solution.

implementation? lJ 3 Granules 1 part by weight of the present compound (■), 5 parts by weight of calcium salt (70), 89 parts by weight of clay, and 5 parts by weight of Tsurupol 5os9 (anionic surfactant) are mixed and pulverized, then compressed into granules. When using it, add it to water and use it as a treatment solution.

Next, concrete examples of tests to confirm the practical effects of the present compound when treated on various plants will be described. However, the two compounds are not limited to these.

Similarly, in the following test examples, the compound represented by the above formula (1) is represented as the present compound (1), and the compound represented by the formula 2 (It) is represented as the present compound 46!1 (It).

Test Example 1 Growth promotion effect test Fill a 4-inch plastic pot with field soil @.
Sow 10 seeds of Japan Time) and reach the 3-leaf stage.

An aqueous solution of the present compound (1) at a predetermined concentration was sprayed over the entire surface.

Plant height, aboveground fresh weight, and dry weight were measured at the 5-leaf stage. The results are shown in Table 1.

Plant one cucumber in a 4-inch pot (variety:
Two plants of Sumo Hanpaku) were treated with compound (1) at the 1st leaf stage, and the leaf area of the 2nd leaf was investigated at the 5th leaf stage.

The results are shown in Table 2.

Table 1 (Results of rice growth promotion test) As is clear from Table 1, the treatment concentration of compound (I) is 0.
1 to 1 ppm, and the treatment concentration of compound (II) is 10 to 1 ppm.
At a low concentration of 100 pp (1), it significantly promotes the growth of rice.

Table 2 (Kirari growth promotion test results) As is clear from Table 2, the treatment concentration of the two compounds (1) α1-1p1)m
One compound (U) significantly increased the leaf area of Kirari at a treatment concentration as low as 10 ppm.

Ai J11L rooting promotion effect test The main stem of a chrysanthemum (variety: Kankobai), which has grown into a main stem, was cut into 7 to 8 wounds, and the 2 to 6 leaves that had grown on it were also cut by about half. Created a seedling.

Only the cut portions of the above-mentioned cuttings were immersed overnight in a predetermined concentration water solution containing the present compound (I) and α-naphthalene sodium acetate (NAA), and then placed on a pre-prepared bed (akadama soil) at regular intervals. Sashi.

Irrigated from above. Leave it in a greenhouse at 25℃ and process 22
A day later, I dug it out and observed and investigated the rooting level. The aboveground part was also observed and investigated in the same way.

The results are shown in Tables 3 and 4. The evaluation criteria in Table 3 are as follows.

Judgment criteria: 5...The development is thick 4...l medium 3...I small 2...l small 1...only callus formation is 0...l I NoneTable 3 (Chrysanthemum) As is clear from Table 3, the two compounds (1) are NA
Even when treated at a low concentration of A/100, it has a rooting promoting effect superior to that of NAA.

The criteria for evaluation in Table 4 are as follows.

Judgment criteria: 5... Leaves are green and new leaves have developed 4.
・The leaves are green as they were when they were treated. 6. Some of the leaves are yellowed. 2. Half of the leaves are yellow. 1. All of the leaves are yellow. Yellowed Table 4 (Anti-aging effect on the aerial parts of chrysanthemums) As is clear from the results in Table 4, one compound (1) has the same effect as the control drug NAA.
Even at a low concentration of '/+ 00, it has a superior anti-aging effect on the aboveground parts.

In view of the results in Tables 3 and 4, this compound.

It can be said that it has both a rooting promoting effect and an anti-aging effect at the same time.

Test Example 3 Test for suppressing aging of primary leaves of rapeseed beans A 4-inch plastic pot was filled with field soil, 5 seeds of rapeseed were sown, and after germination, 2 plants with good growth were left and the others were thinned out.

After the first true leaves developed, an aqueous solution of Compound (1) at a predetermined concentration was sprayed over the entire surface, and 20 days later, the degree of depletion of the first leaves was observed and investigated.

The results are shown in Table 5. The criteria for determining the degree of deterioration of primary leaves are as follows.

Judgment criteria: 5...Complete greening with no impoverishment) 4... Partial yellowing 3... 5ON yellowing 2... Totally impoverished 1... Totally impoverished and 1 part It gL Table 5 As is clear from Table 5, the treatment of primary leaves of rapeseed with this compound has a remarkable anti-aging effect.

``One embryo'' ``One carp'' - test for improving the shelf life of cut flowers An aqueous solution of a predetermined concentration was sprayed over the entire surface of flowering flowers planted in 4-inch plastic pots. The day after spraying.

The 10 flowers that bloomed in each ward were used as cut flowers.

It was placed in a 100 sd beaker and filled with 50 m of distilled water.

The cut flowers were stored in a bright room at 25°C, and the degree of damage to the cut flowers was observed and measured 8 days after spraying. The results are shown in Tables 6 and 7.

The degree of damage to the cut flowers was evaluated based on the following criteria.

Judgment criteria = 5...No discoloration of petals, as before treatment'1
) 4... Part of l is discolored 5... 511X of l is discolored 2... All 5 parts of l are discolored 1... All of I is discolored and some of them are separated Table 6 (Test results for North Pole) Table 7 (Test results for North Pole) . Even when processed at a low concentration, the same amount of food has a superior shelf life effect (freshness maintenance).

Reference Example 1 Chlorophyll Retention Effect After spraying one aqueous solution of compound (1) and benzyladenine IfL (concentration 10 ppm) to rice at the 7th leaf stage, the 5th leaf of the rice was cut off at regular intervals. Sections of the central part 23 of the leaf blade of the first leaf were prepared, and 10 pieces of the sections were floated in a Petri dish with a diameter of 7 (7 mm) filled with distilled water. After keeping the petri dish in the dark at 30℃ for 72 hours,
Extract chlorophyll with acetone and colorimetrically measure it with a 1-minute spectrometer needle (
The wavelength was measured at 66 [Tnm].

The results are shown in Table 8.

10,000 In the same manner as above, two compounds (If) were prepared using an aqueous solution with a concentration of 10 ppm, and the day after spraying (1
The effect of post-cleavage (after 1 day) was compared with the effect of an aqueous solution of compound (1) and benzyladenine at a concentration of 10 ppm.

Table 8 The numerical values in Table 1 indicate OD 660 am values.

From Table 9 Tables 8 and 9, it was observed that the chlorophyll retention effect of the two compounds was lower than that of benzyladenine. However, the above Test Example 2. Considering the results of test νl13 and test example number.

It is recognized that this compound has practically excellent effects in preventing aging and preserving freshness.

Patent applicant: Nissan Chemical Industries, Ltd.

Claims (1)

[Scope of Claims] 2α, 3α, 22s, 25B-tetrahydroxy-24B-ethyl-B-homo-7-oxa-5α
-cholestan-6-one or. A plant growth regulator containing 5β,228.2313-)rihydroxy-248-ethyl-B-homo-7-oxa-5α-cholestan-6-one as an active ingredient. (2) %Claim tic1 which is a growth promoter
The plant growth regulator described in Section 1. (3) The plant growth regulator according to claim 1, which is a plant rooting promoter. (4) The plant growth regulator according to claim 1, which is an agent for preventing aging and preserving freshness of plants.