JPS59118132A - Hydrogen clearance type measrurement of body fluid amount - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION This invention is a hydrogen clearance type body fluid flow ++t? which is used in animal experiments to measure thinning of body fluid flow ffl in blood vessels and lymph glands due to the action of drugs, etc. i! U
This paper relates to improvements in the method of determination.

The conventional hydrogen clearance method for body fluid flow measurement involves saturating biological tissue with hydrogen, then measuring the time it takes for this hydrogen to be flushed out by body fluid flow, and calculating the body fluid flow rate within the biological tissue from this measured value. It is something. Hydrogen concentration in living tissue can be controlled by changing the so-called polaro current caused by ionization of hydrogen by using a related electrode inserted into or in contact with the tissue and an indifferent electrode placed in another part of the living body. The body fluid flow rate in the tissue is determined from the half-life time of this current value. Traditionally, methods for generating hydrogen in living tissues include inhaling hydrogen gas through the respiratory tract or injecting hydrogen-dissolved liquid into blood vessels, but recently, methods for producing hydrogen in living tissues have been used. Current-carrying electrode (cathode)
A method has been proposed in which hydrogen is generated by the principle of electrolysis by inserting or contacting the tissue and passing a weak electric current through the tissue.

By the way, in the conventional hydrogen clearance acid body fluid flow t,21 measurement method, the body fluid flow rate is calculated from the half-life time due to clearance after hydrogen is generated in the test tissue, as described above. It was not possible to continuously measure the current (it). In other words, for example, in the method of generating hydrogen using the principle of electrolysis described above, a minute current from a constant current device 1 shown in FIG. After hydrogen is generated in the tissue 2 by flowing between the inserted cathode 3 and the anode 4 in contact with the living body, the switch 5 is turned off to stop the electricity supply. The change in the polaro current flowing between the electrode 6 and the indifferent electrode 7 in contact with the living body is measured as a clearance curve a shown in FIG. 2, and the flow rate of body fluid in the living tissue 2 is calculated from the half-life time t. Therefore, in order to obtain one measurement value, at least the time required to generate a certain amount of hydrogen and the time required for the hydrogen to be reduced by half due to clearance are required, and the somatic flow sensitivity within the living tissue 2 is required. It is impossible to measure continuously. Note that 8 in FIG. 1 is a Polaro current measuring device.

The present invention solves the drawbacks of the conventional hydrogen clearance measurement method described above and provides a method for continuously measuring the flow rate of body fluid in living tissues and lumens.

It has long been known that the half-life time due to clearance of hydrogen generated in biological tissue has a correlation with the flow rate of body fluid within the tissue, and the conventional body fluid flow measurement method described above utilizes this correlation. However, as a result of detailed research by the inventor of the present application, a cathode for hydrogen generation and a dielectric electrode for detecting polaro current were inserted into living tissue or a lumen at regular intervals to maintain a constant value at the cathode. When a minute current of
It has been found that there is a certain correlation between the polaro current value of the electrode and the flow rate of body fluid in the tissue or lumen when the current is applied. F,! IJ is a polaro current detection electrode in which a fine electrode is inserted into a living tissue or a lumen, and a weak constant current of several microamperes or less is continuously passed through the electrode and placed near it. When measuring the hydrogen concentration, it was found that when the body fluid flow rate in the tissue or lumen increases, the Polaro current (11'J) decreases, and when the body fluid flow rate decreases, the Polaro current value increases. It is recognized that there is a certain correlation between the polaro current value measured in this case and the body fluid flow rate in the tissue or lumen, provided that the distance from the +'fZ electrode and their structure and dimensions are constant. It was done.

This invention was made based on the above-mentioned findings, and a detection needle 12, which has a cathode 9 and a related electrode 10 spaced apart from each other as shown in FIG. It is characterized by measuring the body fluid flow rate in the tissue or lumen by inserting it into the tissue or lumen and measuring the polaro current while continuously passing a small constant current through the cathode 9. It is something. The polaro current measured by this method has a certain correlation with the body fluid flow piece 1 in the tissue or lumen to be measured, and by calibrating the characteristics of the detection 0112 used outside the body, It was also possible to measure I'1, the absolute flow of body fluid within the test tissue or lumen. In this case, conventional devices can be used as the constant current device and the polaro current measuring device, and the cathode 9 in the embodiment shown in FIG. 3 is a PT-Ir wire with a diameter of 75 microns, is a 25 micron Pt-1r line.

As described above, the present invention provides a simple method that can continuously measure the flow rate of body fluids in living tissues or lumens based on new knowledge, and it aims to improve the storage effect and functional changes in living tissues. This is an extremely suitable method for researching such matters.

[Brief explanation of the drawing]

Figure 1 is a block diagram schematically showing a hydrogen clearance type body fluid flow (Tl measuring device), Figure 2 is a diagram showing a conventionally measured hydrogen clearance curve, and Figure 3 is a body fluid flow rate adopted in the method of the present invention. 1 is a perspective view schematically showing an example of a measurement electrode. In the figure, 1 is a constant current device, 2 is a biological tissue, 3 is a cathode, 4 is an anode, 6 is a related electrode, and 7 is an indifferent electrode. City pole, 8 is Polaro current measuring device, 9 is cathode, 10 is electrode, 11 is insulating material, 1
2 is the detection side. Agent Patent Attorney Masaru Nishi [1

Claims (1)

[Claims] (1) Microscopic passages in living tissues or lumens of animals other than humans? A cathode for il and a electrode for measuring polaro current are inserted or felt in close proximity to each other, and the polaro current value is measured while passing a constant current through the cathode for energization. A hydrogen clearance method for measuring body fluid flow rate, characterized by continuously measuring the flow rate of body fluid in a 4Ni tissue.