JPS5910791B2 - Saikindo Teiyousouchi - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a bacterial identification device, and more particularly, the present invention relates to a sealed bacterial identification tube containing an identification medium.

Identification of bacteria is used in clinical tests for infectious diseases, and the identification of intestinal bacteria in particular is becoming increasingly important.

Bacteria are generally identified by their biochemical properties, and there are many documents both domestically and internationally, but the most basic one is I
Bergeys Mamual J (USA).

Several devices aimed at identifying intestinal bacteria have been developed in recent years based on the basic identification reactions described in these documents.

For example, API (API Products, USA), E
nterotube (Hoffman-LaRo
che, USA) and lViinitek (B B
L, USA) are representative examples.

However, these conventional bacteria identification devices were of an open or semi-open type, and therefore used a dry medium or an agar medium.

Therefore, in cases where the colony size is small, a sufficient reaction is often not observed even after 24 hours of culture, and errors in identification are inevitably likely to occur.

On the other hand, there was a drawback that a considerably long time (for example, 48 hours) was required to obtain a sufficient reaction.

Furthermore, with conventional open-type or semi-open-type bacteria identification devices, it has been impossible to identify anaerobic bacteria, or it has been complicated to use other auxiliary devices.

Furthermore, conventional open or semi-open devices, even those using liquid media, pose problems of bacterial contamination;
Inevitably, many operations were complicated.

An object of the present invention is to provide a sealed tube for bacteria identification that eliminates the drawbacks of the conventional tubes for bacteria identification.

A device that achieves the object of the present invention consists of a substantially colorless and transparent bottomed tube containing a freeze-dried identification medium, capped and sealed with a rubber stopper, and the inside of which is maintained under negative pressure. and the rubber stopper has flexibility and elasticity to the extent that it can be penetrated by a needle such as a syringe and maintains a sealing function within the tube even after its removal. This is a tube for use.

Next, a specific example of the bacteria identification tube of the present invention will be described in detail based on the drawings.

An object of the present invention is to provide a sealed tube for bacteria identification that eliminates the drawbacks of the conventional tubes for bacteria identification.

The present invention includes a lyophilized identification medium, It consists of a substantially colorless and transparent bottomed tube sealed with a rubber stopper, and the inside of the tube is kept under negative pressure, and a needle such as a syringe is inserted through the rubber stopper to obtain a pure culture to be identified at °C. This is a sealed tube for bacteria identification into which bacteria can be introduced.

Next, a specific example of the bacteria identification tube of the present invention will be described in detail based on the drawings.

FIG. 1 is a sectional view of the tube for bacterial identification of the present invention.

The tube body 1 is made of substantially colorless and transparent glass, synthetic resin, or the like.

The tube body 1 is filled with an identification medium 2, and the opening of the tube is sealed with a rubber stopper 3.

The identification medium 2 is preferably a liquid medium basically containing carbohydrates such as glucose, proteins such as peptone, and inorganic salts, and the composition needs to be changed depending on each test method.

Furthermore, when using a saccharide that is particularly easily decomposed in a liquid state in the culture medium, the culture medium may be freeze-dried, and sterile distilled water or the like may be added with a syringe or the like through a stopper immediately before use.

The stopper 3 is a syringe needle or similar material that can be pierced through it, but it must be able to maintain a constant level of negative pressure in the tube even after the needle is removed. It is necessary to have flexibility and elasticity to the extent that the tube can be passed through and maintain the sealing function within the tube even after its removal, and a rubber material such as butyl rubber is preferable.

The degree of negative pressure is determined by taking into consideration the amount of colony suspension added into the tube with a syringe, the amount of gas generated during culture, etc.

Clostridium tetany (Clostridium tetany)
A gas such as N2 or CO is introduced in place of air in the tube used to identify anaerobic bacteria such as P. tetani.

Also, when identifying aerobic bacteria that are more likely to propagate when mixed with a numerical coefficient of CO, part of the air is replaced with CO2.

It goes without saying that the bacteria identification tube configured as described above is completely sterilized.

The identification reagent is mixed into the identification medium from the beginning during the identification reaction as usual, or added with a syringe or the like after culturing.

For example, in a phenylalanine deaminase test, if a ferric chloride reagent is added after incubation, the color turns dark red if positive.

Also, in the indole test, if Kobok's reagent is added after culturing, the color turns red if positive.

On the other hand, in a glucose test (carbohydrate decomposition test), petyl thymol blue or phenol red is added as a reagent to the identification medium from the beginning.

If positive, buterthymol blue changes from blue to yellow, and phenol red changes from red to yellow.

Next, a method of using the bacteria identification tube of the present invention will be explained.

This device can be used immediately for identification operations.

A single colony suspended in physiological saline or an appropriate medium, or a pure culture of bacteria 5, is passed through a stopper using a syringe 4 or the like, and a predetermined amount of the bacteria is added to the identification medium (Fig. 3).

Incubation at about 37° C. for 24 hours provides a sufficient reaction for identification, especially when a liquid medium is used.

As a result, if a reagent is added to the device from the beginning, the change in color can be observed either as is or after the reagent is added.

Since the tube for bacterial identification of the present invention is of a sealed type, a liquid medium can be advantageously used, thereby shortening the time required for determination, and improving the certainty and reliability of identification.

Furthermore, anaerobic bacteria can be easily identified using this device alone without using any other equipment.

Furthermore, since it is a sealed type, there is no problem of bacterial contamination, and it has the advantage that operations such as bacterial inoculation are extremely simple for a sealed type.

The tube for bacterial identification of the present invention depends on the order of identification.
It is convenient because test items can be freely selected, test items can be easily omitted when the bacteria are predicted, and test items can be increased during identification.

[Brief explanation of the drawing]

FIG. 1 is a sectional view of a specific example of the tube for identifying bacteria of the present invention. FIG. 2 is a sectional view of a specific example of the tray for bacterial identification of the present invention. FIG. 3 is a partial cross-sectional view showing a state in which a bacterial suspension is being added to the tube for bacterial identification of the present invention using a syringe. 1 is a tube, 1' is a tray, 1 is a well, 2 is an identification medium, and 3 is a stopper. -171-

Claims (1)

[Claims] 1 Consists of a substantially colorless and transparent bottomed tube containing a freeze-dried identification medium and sealed with a rubber stopper, the inside of which is maintained under negative pressure, and the rubber stopper is suitable for use with syringes, etc. 1. A tube for bacterial identification, characterized in that it has enough flexibility and elasticity to allow a needle to be pierced through the tube, and to maintain a sealing function within the tube even after the tube is removed.