JPS5879160A - Clinical inspection plate - Google Patents

Abstract

PURPOSE:To obtain an inspection plate ensuring a clear coagulation in an inspection such as an antigen-antibody coagulation reaction by arranging into a synthetic resin substrate a hydrophilic material hard or unable to dissolve into water which moves to the surface from the inside thereof by nature. CONSTITUTION:A hydrophilic material hard or unable to dissolve into water, which moves to the surface from the inside of a resin layer (the one with a molecular weight of less than 500 such as alkylester make of glycerin monostearate and sucrose) is arranged into a synthetic resin such as polystyrene at a rate of 0.2-10pts.wt. per 100pts.wt. of the resin to make a serologic and immuno logical inspection plate. A liquid of a sample serum or the like and a reagent are placed on a plate shaked and then allowed to stand. This generates a clear coagulation image quickly when the sample contains an antigen, chemicals and the like which will causes a coagulation reaction with the reagent. The hydrophilic material in the resin layer makes the surface of the plate hydrophilic but elutes never interrupts the coagulation thereby enabling a highly sensitive detection.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a clinical test plate used for serological or V1 immunological investigation. l b' is the original IA, and it is carried out using #concentration reactions caused by body reactions, such as red blood cell agglutination reaction, Novux V Higashi reaction, carbon agglutination reaction, silica α1゛q1 anti1F, il7. Exists.

These use red NU bulbs, '7''j' ox, carbon, silica, etc. as a partner. %, i, , i1, □+1 i1, and sample J (11i'
By mixing W on ll++611 and 111 rivers, we observed the reaction and determined that the antigens or antibodies adsorbed on the six faces of the 1i body were It is determined whether an antibody or I antigen that specifically reacts with the sample is present in the sample serum compared to the standard immu.

Agglutination reaction based on antigen-antibody reaction
The degree and specificity are greatly affected by the surface condition of the carrier, the adsorption and binding state of antibodies or antigens, but it is also clear that the surface condition of the plate on which the sample serum is mixed has a considerable influence. There is C.

Properties that should be possessed by a plate for clinical testing are that it does not swell or absorb water, is water resistant, has strong water drop resistance17, does not inhibit antigen-antibody reactions, and does not agglutinate. For example, it is easy to observe the condition.

Glass plates have been used in conventional plates for clinical tests, but the disadvantages of glass plates are that they are easily broken and difficult to process, such as coloring, and that it is difficult to observe the state of convergence.

In view of the following points, the present inventors made extensive efforts to improve clinical ultra-blue plates, and finally completed the present invention.

Summary of the present invention 11 In the synthetic resin base material, it has surface migration properties and is resistant to water! 1Ili: A clinical test plate containing a soluble and non-bathable hydrophilic substance. .

Next, we will explain in more detail the Plate V for Clinical Inspection.

Examples of synthetic resins constituting the base material include polystyrene, polymethyl methacrylate-1, polyvinyl chloride,
polycarbonate, acrylonitrile-styrene copolymer, stellate) (water maleic copolymer, acetyl bunarcel IJ-su, butyralized polyvinyl alcohol, borisol pyrene, polyethylene, boron IJ-lI-
Mel J-lupenken-1, ethylene-propylene co-i1i
Combined, Ho'J'' Narenteleftale 1, Polybunashi//Bure, 7talate, etc. are suitable, and these 'f(
＋Number 4ヲ】'(Use by mixing [7 may also be used.
Afterwards, a stabilizer may be added.

The base material is generally used in the form of a film, sheet, or plate.

In the synthetic resin base material, there is surface migration f1. IJ is done.

If it has surface migration property, l, [, Q over time (synthetic tree 111
T〕+! ;The ability to move from the inside of the material to the surface mJ 'a:
This refers to the case where it is mixed with a synthetic resin and molded into a group (J) and then released from the inside to the surface of the group (4) over time. Specifically, insoluble refers to a case where the solubility in 100 y of water at 20° C. is 5 y or less.

Such hydrophilic substances include, for example, partially esterified polyhydric alcohols such as glycerin monostearate 1, glycerin monooleate sorbitan monostearate, sorbitan monooleate, hentaerythritol monostearate, and alkyl esters of sucrose. There are amine compounds such as oleyl ethanolamine, stearyl ethanolamine, etc. In particular, glycerin monostearate, glycerin monooleate, sorbitan monostearate, sorbitan monooleate, etc. with molecular resistance of 500゛ or less can be used. i 0 The synthetic resin base material has surface migration properties and has a resistance of 1 t to water.
In addition to ia or an insoluble hydrophilic substance, a hydrophilic substance that has poor surface migration property l-< and is hardly soluble or insoluble in water may be contained. Table 5 - Poor surface migration means that the surface does not show a member that migrates from the interior of the 4☆j1 bar base to the surface over time, or
1 (4 tastes) that it shows only a slight amount of
Polyvinylpyrrolidone, partially saponified polyvinyl acetate, partially acetalized polyvinyl pyrrolidone, cellulose, cellulose acetate, methyl cellulose,
Partial esterification products of ethylcellulose, hydroxy-7propylcellulose, polyacrylic acid, polyglycerin, etc., with a molecular weight of 5 + 10 J -, especially polyethylene with a molecular weight of 800 to 2 (l l) 0 Partial esterifications of ogizide and polyglycerol are suitable for use.

It has surface migration properties and has a 1. The distance is also 1. < represents an insoluble hydrophilic substance, which is a group U''a: The constituent synthetic resin 1001 (it is preferable to use the proportion of 902 to 10 parts ILL, and the surface migration f'l is poor 1. <, has a surface migration property of 1 l + aqueous physical property of sparingly soluble or insoluble in water, and is sparingly soluble or -6 to 0.01 to 1 part by weight of the insoluble hydrophilic substance in water. Preferably, it is used in a proportion of 10 plate parts.

In addition, a support layer made of paper, scrap metal, next month, etc. may be provided on the surface of the synthetic resin base material. If necessary, printing may be applied to the surface of the synthetic resin base material. In the plate for clinical testing of the present invention, the hydrophilic substance contained in the synthetic resin base phase, which has a hydrophilic property and is poorly soluble or insoluble in water VC, is contained in the synthetic resin base phase. Because it migrates to the old surface and forms a hydrophilic thin film, when performing serological or immunological tests using antigen-antibody anti-L[-,
When the test solution or sample serum is applied to the light surface, -C also does not swell or pre-absorb, and the preferred spread is 9. Since the hydrophilic substance Q1 in the synthetic water resin base material is unnatural, even if it migrates to the surface, it will not inhibit the antigen 91-body reaction. If you use this rate (for clinical blueprints),
It has advantages such as excellent sensitivity in agglutination tests and less possibility of non-specific agglutination reactions.

Clinical 4A investigational play I IJ of the present invention: , Anti-1+iC
It is possible to use the agglutination reaction with other antibodies and apply it to all clinical proteins, for example, C1, iz reaction +1 protein (CR
P) Test, Anti-Stregtridin () titer (A S L
O) test, rheumatoid factor (RA) test, antinuclear antibody (LI
l, :) test, thyroid autoantibody ('1'A) test, Nu
Heart 4/Si 111] -1 ABO blood type determination test, Rh blood type ward, consanguineous cross-matching test, immunocrypurin (I
It is suitable for use in B, A, M, I-)) measurement tests, etc. .

Example 1 Boristile 7100, commercial weight part 17 (, glycerin monostearate 5 g l1141~, carbon black 1
．． III41117.1 = + composition containing 5 layers of
111111 Shun the pellet/l oUsing this pellet, injection mold the bait 130'%+, ll
A black clinical 4fq lIr- plate with a smooth surface of 11 yk+ was obtained. Using this plate, RA test i- for rheumatoid factor secretion was performed with 1tΔ It was carried out using hard I(r).

Glycine*: Kansui mild solution 1. (Take 1 ml of negative and positive blood in a small test tube, add 1 drop each of negative and positive blood (fl)
, 05 u+l), mix well, /(-,
Then, 1t1 (1(]/I/) drops F of each of these diluted blood 7111 were added to separate compartments of the clinical test plate.
did. Adsorption of human denatured T-globulin into a liquid / 1 drop of a reagent consisting of Narene latex (] Ope)
After adding to each compartment, mix well with serum and make a diameter 25
“The molten droplet spread out in a circular shape.

Hold this clinical test plate with both hands and move it gently for about 1 minute. In addition, the spreadability of the t1 father was good, the appearance of aggregates in the positive serum was more rapid, and a clear agglutination image was observed.

Examples 2 to 3 Polystyrene 100 i Hi i φ, 14 parts of glycerin mosedearate 3 parts, 2 parts by weight of polyenalene A xide (molecule, Q] 500), Carpump 22225 parts by weight
1 part of the composition (Example 2), and 1 part of polystyrene (4 parts of 1O heavy R, 1 part of glycilin monos J-alley 3-fold h, 2 parts of pentagly heptaline monostearate, M parts of horn) - A clinical plate was molded in the same manner as in Example 1 using a 2.5 part Bf composition (Example 1), and carried out in 114 and 19. On the same day as I (2017), I applied RAAS 1 to detect rheumatoid factor.

As shown in Example 2.3 of Table 1, human clinical test plates have good spreadability of HT, and the JJJ and WAT1 horns of aggregates in positive serum are rapid.
And at opening 1, the agglomerated image is 111 Ir.

17 calibration polystyrene + 011 i1+ ILlN-,
11I River 12, injection molding resin 1311'%n, 1it
145%, thickness 1% (D'FrRft plate) was molded.Using this plate, we are conducting an RA test for detecting rheumatoid factor in the same manner as in the experimental birds.

In the column of ratio U in Table 1, the spreadability of fusion tl)ll is poor, and the appearance of M and East mass in positive serum is = 1
0- It was slow and the aggregation image was unclear.

The agglutination determination time refers to the time until an agglutination image that can be determined is obtained.

patent applicant Sekisui Chemical Co., Ltd. Representative: Mototoshi Fujinuma -I+- 320-

Claims (1)

[Claims] 1. Plate for clinical examination, characterized in that it contains a hydrophilic substance that has surface migration properties, is resistant to water, and is not vinegar-free in a synthetic resin matrix. A play for clinical building 11 according to Patent No. 1-Claim 1, which has poor surface migration properties and has been known to have hydrophilic substances that are dissolvable or insoluble in water.