JPS5876750A - Data processor for electrophoresis - Google Patents

Abstract

PURPOSE:To enable a correct analysis with an accurate detection of the boundary section between ion component zones of different types by automatically changing computation conditions according to detection signal levels. CONSTITUTION:A data processor 3 of an electrophoresis analyzing system 1 reads detection signals P from an equispeed electrophoresis analyzer 2 every short time of t sec to calculate a mean M and then, a time variation value of the value M to be compared with the threshold variation value D. Then, a time gradient G is calculated and compared with the threshold gradient value S. When the value G exceeds the value S and the value V exceeds D immediately thereafter, this, it is judged, points to the boundary section between the ion component zones of different types. By this procedure, boundary sections are detected between the leading ion L and the ion component I1, the ion components I1 and I2, the ion components I2 and I3 and the ion components I3 and the terminal ion T respectively to obtain the potential gradient value and zone length of each ion component zone. Thus, all the components are analyzed separately and the results supplied to a plotter 4.

Description

DETAILED DESCRIPTION OF THE INVENTION Regarding the data processing process W for aerophoresis of the present invention, more specifically, a predetermined arithmetic processing is performed on a signal from a detector of an electrophoresis analyzer to analyze the boundary area between different types of ion component zones. The present invention relates to an electrophoresis data processing device that is capable of automatically changing condition parameter values for the arithmetic processing according to the signal level from a detector when detecting.

Since irregular noise components may be mixed into the signal from the detector of the electrophoresis analyzer, the noise components are canceled out by calculating the average value of the signals for each predetermined time period. The length of this predetermined time is determined depending on the frequency component of the signal, and to put it simply, if the signal changes suddenly and
l can be short, and if it is loose, it can be relatively long.

If the predetermined time is taken when the signal change is sudden, the signal change will be averaged and correct information cannot be obtained; conversely, if the signal change is gradual and the predetermined time is If it is made short, there is a disadvantage that the probability of noise component mixing increases. Therefore, it is important to select an appropriate time for averaging according to signal changes.

However, in the conventional electrophoresis data processing device, the length of the predetermined time in U1 analysis is fixed.
There is no μ problem when the signal changes due to each component contained in one sample are all sudden or all are gradual, but when there is a mixture of sudden and gradual changes, the above-mentioned problem occurs. This could cause some inconvenience.

On the other hand, in order to detect the boundary 815 minutes between different types of ion component zones from No. 48 from the detector of the electrophoresis analyzer, the time change of the average value I = is the peak.
3. The determination of this peak portion is based on the fact that the time change value of the average value exceeds a predetermined threshold change value and that the time gradient value of the time change value exceeds a predetermined threshold gradient value. This is done on the condition that it exceeds the It is usually preferable that the threshold change value and the threshold slope value U are set large if the signal from the detector changes rapidly, and relatively small if the change is gradual.

However, in the conventional electrophoresis data processing device, the threshold change value and the threshold slope value in one analysis are identified by an identification formula, so as in the case of the averaging time described above,
If the h components contained in one sample include those exhibiting a sudden signal change and those exhibiting a gradual signal change, there is a possibility that a stagnation problem may occur.

In view of the above circumstances, at least one of the predetermined time, the threshold change value, and the threshold slope value is automatically set to another different value during one analysis. An electrophoresis data processing device W having a structure that can be modified is provided.

Hereinafter, this invention will be explained in detail based on embodiments shown in the drawings.

(1) shown in Fig. 1 is an example of the configuration of an electrophoresis analysis system, which includes an isotachophoresis analyzer (2), a data processing device (3) such as a microcomputer, and a printer.
A plotter (4) is successively installed.

U5 potential gradient detector as detector of electrophoresis analyzer,
There are various types of detectors such as heat detectors and conductivity detectors, and the present invention can be applied to those chairs, but for the sake of explanation, the isotachophoresis analyzer (2) has a potential gradient detector. shall be taken as a thing. Also, does the signal from the potential gradient detector look like the one shown in Figure 2? Totosu.

The data processing device (3) μ reads the signal l) of the detector [) every t (for example, 0.1) seconds for a short period of time, and every time n pieces are read, it takes a predetermined time W (= L x n ). An average value M is calculated every second. Further, a differential value of the average value M, that is, a time change value V is obtained, and its temporal eclipse 1tiv is compared with the threshold change value . Furthermore, the differential value of the time change value ■, that is, the time gradient value (] is calculated, and the time gradient value G and the threshold device ++'+ 8 are subjected to a soft calculation. Then, the time arrangement + 14.G is the red gradient value. S
When the time change value ■ exceeds the threshold change value after rk, the signal P from the detector and the predetermined set values p, , p, and are compared, and the signal P is the set value Pr.
Until reaching the above predetermined time w, the threshold change value and the threshold slope value S are respectively MWI (for example, 0.2 seconds),
D□ (e.g. 15 Q pv/see ) and Sl
(For example, 150 μv/-"). The stiffness is a calculation processing condition parameter value that is suitable when the signal P changes suddenly. W, threshold change value and threshold slope value S are respectively set as fi, W2 (for example, 2 seconds), D2 (
f? : For example, 100 μV/sec) The setting is automatically changed to S2 (for example, 15 μV□2). These calculation processing condition parameter values are suitable when the L signal P changes gradually.

When the signal P exceeds the set value P2, it automatically changes the W, D, and S to the Wt e Dlt S t
IC model. In addition, W1+ W2+ 01+ D2+
S1+Sg+Pl+P2μ, which is set by roughly predicting the waveform of signal P.

As shown in Figure 2, if this device (1) is modified, the boundary between the leading ion (T) and the 8Ill ion component (It) of the sample, the first ion component (11) and the The boundary between the second ionic component (I2) and the eighth ionic component (I3), the eighth ionic component (I3) and the terminal ion ('i')
) is properly detected. First ion #k
.

Noise (α) is included in the signal of minute (Is).
Assuming that the processing parameters 'frWt and DI+81 are used, the processing time is approximately the same as that of the boundary between the second ion component (12) and the eighth ion component (I,). Although it is a magnitude that becomes a change value and a time gradient value, the time for U-Ser equalization is W2 in this device #/(1), which is sufficiently long, so the averaging is removed. However, if the conventional device ij is set to III and the calculation processing condition parameters are fixed to Whlh and Sl, the noise (α)
This can be mistaken for the boundary part of ionic components. Conversely, the calculation processing condition parameters are W2 + D z + b
If it is fixed at z, the boundary b1- between the second ion component (I2) and the eighth ion component (13) will be missed.

The data processing device 11131μ, after correctly detecting the boundary between different types of ion component zones as described above, obtains the potential gradient value and zone length of each ion component zone, analyzes each component, and prints the results. Output to plotter (4).

As understood from the above explanation, the average value of the signal per predetermined time and the time change value of the average value with respect to Itami from the electrophoresis data processing device and the detector of the electrophoresis analyzer of the present invention. and calculate the time gradient value of the time change value, and compare and calculate the time change value and the time gradient value with respective predetermined threshold change values and threshold gradient values,
In an apparatus for detecting the boundary between different types of ion component zones based on these calculated values, there is a comparing means for completely comparing the signal value from the detector with a preset value, and the above-mentioned comparison means based on the output of the comparing means. Predetermined time.

a condition changing means for automatically changing the setting of at least one of the threshold change value and the threshold slope value to another different value, thereby automatically changing the arithmetic processing condition according to the signal level from the detector; Since it is characterized by being changeable, it becomes possible to detect the entire boundary between different types of ion component zones more accurately than conventional methods, and therefore, correct electrophoretic analysis can be performed.

[Brief explanation of drawings]

FIG. 1 is an explanatory diagram of the configuration of an example of an electrophoresis analysis system including an embodiment of the electrophoresis data processing head of the present invention, and FIG. 2 is a diagram for explaining the operation of the data processing apparatus shown in FIG. 1. It is a graph of each signal. (1)... Electrophoresis analysis system, (2)... Isokinetic electrophoresis analyzer, (3)... Data processing device, (4)
...Printer pull, (DI) (DI) -L
, Threshold gradient value 1, (8r) (S x )... Threshold gradient value, (Pl) (Pg)... Setting f11h a
-9~ -283-

Claims (1)

[Claims] 1. Calculating the average value of the signal per predetermined time, the time change value of the average value, and the time gradient value of the time change value for the signal from the detector of the electrophoresis analyzer, In a device that compares and calculates the time change value and the time gradient value with predetermined threshold change values and threshold gradient values, respectively, and detects the boundary portion between different types of ion component zones based on the results of these calculations, a detector is used. and a comparison means for comparing a signal value from a predetermined value with a preset value, and automatically changes at least one of the threshold change value and the threshold slope value to another different value based on the output of the comparison means. 1. A data processing device for electrophoresis, characterized in that the arithmetic processing conditions can be automatically changed according to a signal level from a detector by conjugating a condition changing means for changing settings.