JPS5870835A - Adsorptive removal of polar org. compound - Google Patents

Abstract

PURPOSE:To adsorb and remove a polar org. compound rapidly and effectively, by contacting a solution containing the polar org. compound having an ionizable group with titanium dioxide hydrate. CONSTITUTION:A solution containing an org. compound having an ionizable group such as a carboxyl group or a sulfonic acid group such as various fatty acids or alkylbenzene sulfonic acid is contacted with titanium dioxide hydrate prepared by hydrolysis of titanyl sulfate or titanium tetrapropoxide. By this method, the polar org. compound can be adsorbed and removed rapidly and effectively without applying any pretreatment.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a simple method for quickly and effectively adsorbing and removing polar organic compounds from a solution containing polar organic compounds, particularly polar organic compounds having ionizable groups, without any pretreatment. It is.

Domestic wastewater and solutions containing biochemical substances are solutions in which a large number of diverse organic compounds coexist, and when separating trace amounts of nonpolar compounds present in these solutions, it is necessary to avoid the effects of coexisting polar compounds. Have difficulty.

For example, when trying to analyze such a non-polar 4# compound using a Gask 4Mad grab, polar components and high molecular weight components such as proteins are deposited on the column. Column deterioration, reduction in the number of theoretical plates, and poor reproducibility in qualitative and quantitative analysis due to 71 - In addition, adhesion to the detector may cause noise generation, reduced sensitivity, and other inconveniences. When performing graph analysis, it is difficult to set column conditions for simultaneous analysis of monopolar and non-polar compounds, and usually requires complex operations such as setting the optimal conditions for each and sequentially implementing more than one set of conditions. .

Furthermore, in clinical chemistry analysis where it is necessary to analyze nonpolar compounds in body fluids such as blood and urine, it is critical to remove proteins that are present in the greatest amount in body fluids. For example, when a coloring reagent is added to serum, which is a representative body fluid, it becomes cloudy in most cases, making colorimetric measurements impossible. Conventionally, processing has been considered essential, but in clinical chemical analysis, where speed is an essential condition for analytical operations, it is unacceptable for this pretreatment operation to take a long time.

Conventionally, methods known for removing polar compounds such as proteins and enzymes include a pretreatment method using a protein removal reagent, a dialysis membrane method, and an electrophoresis method.Although these methods are all precise separation methods, they require manual operation. This is a temporary anchor and takes a long time.

The present inventors have completed the present invention as a result of repeated studies aimed at alleviating the drawbacks of the conventional methods and establishing a method for removing the low-molecular-weight and low-molecular-weight polar compounds that is easy and quick to handle.

That is, the present invention converts a solution containing a polar organic compound having m and m groups into titanium dioxide hydrate (hereinafter abbreviated as titanic acid).
This is a method in which the polar organic compound is adsorbed and removed by contacting with the organic compound.

The polar organic compound having an ionizable group as used in the present invention is an organic compound having an ionizable group that can be dissociated in a bath to form a cation or an anion. This way'v
IL leaving groups include 1 carboxyl & (-COOH), su, sulfonic acid group (80, H), phosphonic group (PO, H,)
etc. as groups that ionize and have anions, and primary amine, secondary amine, tertiary amine groups, etc. as groups that combine with hydrogen ions and have cations in solution.
Salts bonded to these ionized groups, such as alkali metal salts and hydrochlorides, are also included in the ionized groups referred to in the present invention.Polar organic compounds having these ionized groups include various fatty acids such as vinegared rice,
Alkylbenzenesulfonic acids, phospholipids, various amino acids/@
, proteins, enzymes, etc. can be removed by the method of the present invention. In particular, polar Tll1/A compounds having both a carboxyl group and an amine group (NH, ) in white matter, enzymes, etc., can be removed by the method of the present invention. It can be effectively removed using this method.

Next, in the present invention, the titanic acid used is titanium hydrate with the chemical formula %, and titanyl sulfate (T10804
), titanium tetraisopropoxide (Ti(OCR(C
Hm)t)a), titanium tetrachloride (TiC), etc., which are produced by hydrolysis, have a large specific surface area and are convenient for the purpose of adsorption.

Further preferred is titanic acid produced by the method disclosed in Japanese Patent Application Laid-Open No. 2003-120002. In other words, a hydrated titanium compound having an anatase or amorphous crystal structure mixed with an inorganic acid and water, mixed, extruded, granulated, and dried becomes a preferred titanium #I adsorbent with excellent morphological stability. Particularly, if the granules are granulated and molded by the above-mentioned method or are sized by pulverizing and sieving the granules, they are advantageous in terms of operability when used by filling them into cartridges and the like. The particle size of the granules is preferably goNioooμ, more preferably 100 to S00μ, and iso to OQμ optimal for use in packing into columns and the like.

Next, the method of the present invention will be specifically explained using the drawings. A small cylindrical column (inside the titanic acid).The shape of the column can be arbitrary, but a cylindrical shape is easy to handle.Also, the size can be determined depending on the sample concentration and sample amount. , sample s
It is appropriate to use lN-titanic acid for ~10 ml.

The column can be made of any material as long as it does not denature the sample, but glass or stainless steel have sufficient strength. Inlets and outlets (6) and (7) with filters (3) are provided at both ends of this column, one of which serves as the inlet for the sample and supplies the sample bath liquid (co), for example, with a syringe (1), and the other serves as the inlet for the processing liquid. Exit ζ. The filter (3) may be of any type as long as it prevents the outflow of titanic acid, but commercially available cellulose ester filters and the like can be suitably used. A sample bath solution is put into this syringe, and the processing solution that passes through the titanic acid in the column and comes out from the outlet is collected in a container. The outflow speed is arbitrary, but although it is small, the removal rate is high □ If you take into account the safe time! , about Oml/min is efficient.

As described above, the present invention is capable of adsorbing and removing polar organic compounds f contained in a solution using a very simple method, and the adsorbed polar organic compounds can be recovered by flowing an alkaline solution as an eluent. □ Next, the present invention will be explained in detail using Examples, but the present invention is not limited to the following Examples unless the gist thereof is exceeded.

The titanium granules granulated using a pulverizer were filled with titanate 09 into a cartridge as shown in the figure, and the top and bottom were sealed with commercially available cellulose ester filters. Attach a glass syringe to the sample injection port on the top of this cartridge and add Oml of raw liver fibril/gen water bath solution (fljIB to pg/rnl) mixed with distilled water.
Flow velocity! The titanic acid solution was passed through at rmJ/ml. Collect the oral fluid at the treatment solution outlet, and determine the live liver fibril/gen concentration in the treatment solution using the TOC method.
/mノ. The removal rate of raw liver fibrinogen was 9j%. Similarly, ovalbumin was removed, and the results were summarized in the following table.

It is found that polar substances 5M - particularly amino acids, proteins, and enzymes - are effectively adsorbed and removed.

[Brief explanation of drawings]

The drawings show an example of a device suitable for carrying out the invention! It is something. l・°・Glass syringe Co...sample solution 3...
Cellulose, Sester filter d...Sized titanate S...Stainless steel cartridge 6...Sample injection port a...Processing liquid Taguchi

Claims (1)

[Scope of Claims] (1) A method for adsorption and removal of polar organic compounds, which comprises bringing a solution containing a polar organic compound having an ionizing group into contact with hydrated titanium dioxide to adsorb the polar organic compound. (c) The adsorption removal method according to claim 7, wherein the polar organic compound having an ionizable group is an anoic acid, a protein, or an enzyme. (3) The adsorption removal method according to claim 97 or claim 1, wherein the titanium dioxide hydrate has a particle size of go to 1000μ.