JPS5846314A - Filter optical system for microscope - Google Patents

Abstract

PURPOSE:To obtain the maximum contrast with one filter for many dyed coloring matters, by arranging the filter, whose angle to the optical axis is variable, in the optical path from a light source to an eyepiece. CONSTITUTION:A filter supporting material 6 is placed on a filter receiver 5 of a microscope base 3. In the supporting material 6, a filter 7 is attached turnably around a rotating shaft 8, and the rotating angle is indicated by measures 10. In case that the transmission peak of the filter is matched to the absorption peak wavelength of used dyed coloring matters, the filter 7 is turned to find the position of the highest contrast. Thus, the maximum contrast is obtained with only one filter for many dyed coloring matters.

Description

[Detailed description of the invention] The present invention relates to a filter optical system for a microscope.

In particular, it relates to contrast filters for black and white photography in biological microscopes.

In a biological microscope, in order to observe specimens with very low contrast that makes it difficult to observe the fine structure, the specimen is stained with a dye that has an absorption peak at wavelength λG, and this is dyed with a dye that has a transmittance peak at wavelength λ0. A method is known in which the contrast is increased by illuminating through a filter. However, when implementing this method,
Since the pigments differ depending on the type of specimen, it is necessary to prepare a filter with a transmittance peak at the same wavelength as the absorption peak for each pigment, and as a result, multiple filters can be exchanged and used during observation. Therefore, the operation was complicated and it was also economically disadvantageous.

Therefore, it is an object of the present invention to provide a microscope filter optical system that can handle a large number of dyes with a single filter by utilizing the fact that the peak wavelength of an interference filter changes when the incident angle is changed. This will now be explained with reference to one embodiment shown in the drawings.

1 and 2, l is a conventionally known microscope body, 2 is a light source, 3 is a microscope pace, 4 is a condenser lens, 5 is a filter holder, and 6 is placed on the filter holder 5. A filter support according to the present invention, 7 is a filter attached to the filter support 6 so as to be rotatable around a rotation axis 8, 9 is a fixing device for locking the rotation of the filter 8, and 10 is fixed? This is a scale for indicating the rotation angle or transmission peak wavelength.

The embodiment of the present invention constructed as described above operates as follows. When observing a specimen stained with a certain dye, the filter 7 must be rotated around the axis 8 to adjust the transmission peak wavelength of the filter to match the absorption peak wavelength of the dye used. do it·. To do this, while observing the specimen, move the filter 7 to the shaft 8.
Rotate around L to find the position with the highest contrast, but adjust the scale (rotation angle) so that the peak transmission wavelength of filter 7 is the same as the absorption peak wavelength of the dye used. or wavelength). In this way, it is possible to match the transmission peak wavelengths of a large number of dyeing dyes using one filter 7.

Figure 3 shows the spectral transmittance curves of dyes used for biological specimens, where a shows the case of eosin, which has an absorption peak wavelength around 530 nm, and b shows the case of smeared acid tsukusin, which has an absorption peak wavelength around 550 nm. ing. Therefore, in the case of eosin, a filter with a transmission peak wavelength of 530 nmK! - The observation field with the highest contrast can be obtained, and in the case of acid gas, the observation field with the highest contrast can be obtained with a filter having a transmission peak wavelength of 550 nm.

FIGS. 4 to 5 show examples of interference filters 7 used in the present invention, including A, B, and O.

D, 'B, F, G seven-layer film structure with refractive index of 2.35, 1.35, 2.35,
1.35.

2.35, 1.35, 2.35 and the thickness is λO/
4゜λo/4. λo/4. λo/2. λo/4. λo/
4. The refractive index of the air on both sides is 1.5.

The spectral transmission characteristics of the interference filter configured as described above are shown in Figure 5, and the center wavelength shifts as the incident angle changes, but I, n, and III have an incident angle of 0° and an incident angle of 1
5° and an incident angle of 30′. Therefore,
By appropriately rotating this interference filter, the third
It is possible to obtain transmission characteristics that match the absorption characteristics as shown in Figures a and b.

According to the embodiment configured as described above, by simply rotating the filter around the axis 8, maximum contrast can be obtained for each of a large number of dyes using only one filter. It is also economical and easy to manufacture, as the filter optical system can be configured by simply placing the filter support that supports the filter on the filter holder of a conventional microscope, without having to construct the main body of the microscope. There are some effects.

In the above embodiment, the filter is disposed on the filter receiver, but the filter may be disposed at any other position as long as it can be rotated and fixed in the optical path.

[Brief explanation of drawings]

FIG. 1 is a side view of a microscope incorporating a filter optical system according to the present invention, FIG. 2 is a perspective view of a filter support frame according to the present invention, FIG. 3 is a spectral transmittance curve diagram of an example of a dye, and FIG. The figure shows an example of the configuration of an interference filter used in the present invention, and FIG. 5 is a spectral transmittance curve diagram of the interference filter shown in FIG. 4 as a result of changes in the angle of incidence. DESCRIPTION OF SYMBOLS 1... Microscope main body, 2... Light source part, 3... Microscope pace, 4... Condenser lens, 5... Filter receiver, 6... Filter support, 7... Filter, 8 ... Rotating shaft, 9... Fixing device.

Claims (1)

[Claims] A filter optical system for a microscope, comprising a filter arranged in an optical path from a light source to an eyepiece so as to change the angle formed with the optical axis.