JPS5831987A - Particles for supporting microorganism - Google Patents

Abstract

PURPOSE:Particles for supporting microorganism having extremely improved adhesivity to microorganism, having a particular specific gravity, obtained by attaching an inorganic or organic substance having specific particle diameters to the surface of expanded synthetic resin particles. CONSTITUTION:One or more substances selected from an inorganic substance (e.g., sand, natural ground stone, silas ballon, ceramic, etc.) and an organic substance (e.g., anthracite, coal, coke, rubber, etc.) having praticle diameters <=1/3 of expanded synthetic resin particles and >=50mu are attached to the surface of the expanded synthetic resin particles (e.g., particles consisting of a material such as PS, PE, PP, etc.), to give particles for supporting microorganisms, having a specific gravity of 0.2-1.0. When the particles to be attached to the synthetic resin are concentrated to only the surface of the resin and crosslinking phenomena will not take place, many improvements are attained, for example, the expansion magnification of the resin is made high so that its amount is reduced, the surface of the expanded particles are made rough so that hydrophilic nature is increased and adhesivity to microorganism is improved, and the adjustment of specific gravity of the expanded articles is carried out easily.

Description

[Detailed description of the invention] Regarding the present invention kt-mimic organism support) 1: 1-f- 2.1
j11. <It's a microorganism used for water treatment and HY raw Pρ! Foam synthesis for it (Ill)
Regarding 'i' particles.Furthermore, a Jli (search and/mada L1) with a special IH particle size was attached to the surface of the foamed synthetic resin 11'rj particles, and the specific gravity was 11゛. 6ρ raw 11), which is obtained by adjusting W in the range of 02 to 1.0, relates to the 114th column r l'il ri/ f-.

Traditionally, house j court team - water, kuri 1) 1 water! ! 1) Water treatment techniques 1) have been proposed that cover a wide range of fields such as filtration, sedimentation separation, and biological treatment. Use the body I L, teσ+i
l i11'b, how to effectively perform biological treatment, etc. θ;
is opened) ζ.

In addition, even in fermentation where the production of specific useful substances is carried out as a secondary method, conventional rlv71.
), an alternative method is to collect microorganisms in phase particles.
There are 47.1 proposed ways to drink and do the same thing.

An example of this is the immobilized yeast method, in which the yeast is immobilized on a carrier such as agar.

The eaves lit particles used in these methods are natural light particles.
11' product ('3) - Resin, such as foamed polyethylene, foamed polypropylene, foamed polystyrene, etc., which has already been filled with an Ij absorbent,
Protection? ),,',4. Commercially available particles are used for the purpose of , rc as explained below
There is a problem of 1'l: ability-]-, which makes it unsatisfactory.

The first is that when used as a carrier for microbial water injection 111, it takes time for a microbial film to form on the surface of these particles, and the formation 1 or C;!
The sticking strength of the biofilm to the particle surface is small. That is, since the surfaces of these conventionally known particles are relatively smooth,
It takes a long time for a biofilm to form, and if the biofilm grows to a thickness beyond the point where a biofilm can be formed due to long-term use, particles may form with each other or l-1 particles. ) 1iii agitation with the wall, shearing the water or particles of the particles by air bubbles, ] and living things; part of the model or all of V1 is 4/+11117. It is to be. The inventors have identified this defect on the surface of the particle,
1.
l! J+ and 1) Making the surface shape star-shaped, etc. Solved by IJ+ - Ij, and (S", ', '7), and the resulting effect of culm 1σ was applied to the first generation G]f', but it was not completed. It's not even 5 star.

Second, there is h7+ between the specific gravity of lA:r. When performing industrial/target lr water treatment, the lrl of the raw water to be treated
,l'l night, the size of the treatment tank, the amount of treated water is ↑11 out π1 saving J l! In many cases, it is necessary to change the operating conditions such as the flow pattern of raw water, etc., and when using pressed particles, it is necessary to change the appropriate t and r ratio for Vl, sofI, and L7. Selective use of particles with a specific gravity of 1'71. Beautiful JJ:, I agree a lot. However, at present, when using expanded particles L1 used for water treatment, the specific gravity must be constant. Self 1. Is it OK even when using ν1 pattern fat? , foam magnification 4 - By changing the color, *1lfi adjustment of the specific gravity of the particles themselves is possible (11 (
But, 3-shot? - Since the value of 1x ratio brings about a large change in particle properties, there are restrictions from the perspective of expansion ratio, and therefore it is difficult to arbitrarily change only the specific gravity.

In this situation, we synthesized (/J
1 fat power! The main component is a mixture with 1 organic material, and this 4
・The particles obtained after foaming and 14 hours are hydrophilic, and the sticking force of the material film is extremely large, and the ratio of the synthetic matrix 111' and the inorganic ion material By changing the ratio, we succeeded in obtaining particles with a desired specific gravity at a foaming rate that maintains most of the bubbles in the 4iit particles as closed cells, creating new low specific gravity particles for water treatment. Tori Te 4j1 (Japanese Unexamined Patent Publication No. 56-8989
No. 7).

However, for some reason, these particles are inorganic material PI4 in synthetic resin.
・&Ii is obtained by contact foaming with a high degree of hydrophilicity, and increasing the hydrophilicity by ↑1/increasing the inorganic material's porosity increases the inorganic material to 1 A bridge phenomenon occurs, and the water that penetrates the inorganic material becomes independent air r.!
As a result, the specific gravity of the foamed particles increases and they sink in the water.

4- Furthermore, the IJII synthesis described in -1- (a・+ fat with no 1%
't! rl by the method of kneading r N old, inorganic CI4
If you increase the combination 111 of '4ne1 to non'1;i, 1height4
1-Using bubbles 11" as the rate is Mii-like C.--Problem 1
1". Therefore, the expensive combination 1]ν, 4 including 11iM
Use of AI4+l'l 1) There is a limit increase in setting 1 to rl, and the cost of I!jet child is;
It was 3 high.

The present invention d, by adding further modification 3 to the ``-lower ratio +lj particles''/(with the purpose of solidifying microorganisms t!IFj /l'lwa'l particles, (19 synthetic tree 1
] i'+ i; 1 most of the particles Vj should be applied only to the surface of the resin, and the above 741, 1 phenomenon should not occur ■ High expansion ratio of synthetic resin < t, -c synthesis (3) Roughening the surface of the foamed particles to significantly increase their hydrophilicity and improving the adhesion of microorganisms (0) Specific gravity of the foamed particles This is done for the purpose of facilitating adjustment.

The present invention relates to foamed synthetic resin particles having a diameter of 1/6 or less and 50μ or more of the diameter of the particles on the surface (rll) of the foamed synthetic resin particles.
(And one yuzu selected from organic matter is still rl, and the ratio j11 made by adhering two or more yuzu substances is 02 to 1.0.
11iji of (i; 9 organisms j[I is an exported particle.

The foamed synthetic resin particles used in the present invention are thermoplastic resin particles.
;, 46111ft is foamed to form particles. Synthetic 401 is used to make a foam that has Q-1 closed cells.For IIz, polyethylene, polypropylene, polystyrene, polystyrene, vinyl chloride, etc. are used. be able to. Particle 1 (4-shaped)
-Shape, angle (1 shape.

Circle] 1 type snow (Tl; Kj, foamed formation 4) L
The body itself - C";l;
(Also, it may be t1″l. Size of the foamed synthetic resin particles: 1 Although it varies depending on the purpose of use, the particles may be
't' i17? -"5ze, or tj When used in a fluidized bed, Vl,++'<diameter i is generally ~20 nm, preferably 1~IO++++n] 1; On the other hand, when the particles are used in a fixed bed When using) t, 6 to 20 to 50 t to ensure that jI9″I does not occur during occlusion by living organisms.
a! The foaming j0 of synthetic resin "base" is generally 1.5 to 6o (
1 (1 is used, but it is limited to this range and Q: 1 is not used.'101 Foaming of synthetic resin particles commonly used fcFi, I: 2 to 40) be.

The surface of the foamed synthetic resin particles is 78*i1-. ν) ru fre
t machine 114)) ki sand, natural crushed stone, activity 1 as τI
! ij + perlite.

There are whitebait balloons, gypsum, alumina, and 1° ceramic points. ) 1 nib, with PJ q:,'B
+e7 no 17, V-1 anthrayite, coal, coke, pitch, +Y? There are ion exchangers, fats, rubber, etc. These ir + ``(books' e1 and organic male + Ij l
2. Let's go! ! Zoro J-I 6I J sea urchin that has a fat particle size of 115 or less and 50 μ or more
It is used with adjustment l~. Inorganic substances and iC141 &things'e! If you add 1/3 of the diameter of the foamed synthetic resin particles to d (1), then add 1i to the appropriate size of the particles.
L cannot detect a large number of particles, and cannot detect a large number of particles. Therefore, I/l+
! 1-J- cannot form a good surface in the swamp. In addition, if it is less than 5071, I j < ji will be reached on the surface of the foamed synthetic resin particles.
L<, 2.5 layers, which makes φj easy to bend. -1 These substances may be used alone or &J'
2) can be used in appropriate combinations. The objective of the above f++1 machine is to collect organic substances to obtain microorganism-carrying particles with a specific gravity of Supplementary investigation of powder, etc.;
Any means can be applied to adhering the inorganic substances and 11-organic substances to the 2nd foam synthesis 1st and 1st fat t-'. This can be carried out by dissolving the surface of the foamed synthetic resin particles using an organic solvent such as, for example, and then bringing them into contact with an inorganic substance or organic substance. Another method is to wet an inorganic substance or an organic substance with an organic solvent and then bring it into contact with foamed synthetic resin particles.

In this case, if an adhesive such as an epoxy resin is used in combination with an organic solvent, a strong adhesive effect can be obtained.

fli'2 Adjustment of the specific gravity of particles for supporting organisms d Foamed synthetic resin! The size and specific gravity of the particles and the size and specific gravity of the particles of inorganic and organic substances attached to the synthetic resin particles. , puncture, etc., and this point will be discussed later.

61 The microbial adhesion of particles for supporting organisms is determined by the particle surface -〇
- There is a deep relationship with the state DI, +I', tl'? i';
&: 1 particle table 1111 shows 84 (1 or ``O・da'' or ``
``Irregularity'' is IF, I (then, (+'(4 so 1)'s old-fashioned feeling increases markedly.
On the surface of the In synthetic resin particles, f+t+ HI1. (=3
j7 things 't7 and J + chivalry 't! 1) Allowing the particles to adhere to the surface of the synthetic resin particles means forming countless irregularities on the surface of the synthetic resin particles.1. ,! 1 is created. Therefore,
By using the M't 4 particles of the present invention, sufficient 1j1 microorganisms can be attached to the particles in a relatively short period of time. Regarding this fact, the present invention Nagisa 1, the following 141 (-J', solid, and this is actually ff+E
Shita.

Since the particles for supporting microorganisms of the present invention are generally used in a suspended state in water, the particles are
．． Adjust to 0. In addition, in 1,1, the particle is -1', y,
When used in J4 type water treatment equipment, h'! Child ratio i1
(It is desirable to set it to 05-095.

In order to obtain L11 particles with a predetermined specific gravity (I1), foam synthesis (the particle size and specific gravity of the θ11 layer, the particle size of the inorganic substance, the organic substance (Iz%f particles), the ratio 11 (The relationship between Foaming Synthesis 41) Assuming that both the fat particles and the attached particles are spherical, it can be expressed as follows. In this case, the particles are one layer as shown in Figure 1. shall be.

Diameter of foamed synthetic 4 fat particles: D(n-n) ratio,! ff
:ρ.

Attachment 71-I Particle quality: d (lung) 〃 Specific gravity: ρ□ Porosity: ε Foaming synthesis 4ff + surface area of fat particles: πD2 ... (
1) Projected area of local particle: (π/4)a, l'...
・(2) Effective area of foamed synthetic resin particles: (1-ε)π
D2... (3) attached - 1 particle stuck v! : Total volume of foamed synthetic resin particles and attached x1 particles: foamed synthetic resin 1 )! Total weight of if particles and attached particles J Tsuji: L
fl car'), microorganism-carrying f+'tr-cb
Jt I+'(p, k, I) Substituting equation (4) into equation (7), we get ε-026 when the O1 particles are most closely packed.

As mentioned above, foamed synthetic wood II! -r particles are also old Mf
The approximate formula obtained when assuming that the fat particles are also spherical is the formula 2. It will become.-
1; Using formula (8), the first
An example of calculation when making "l" is as follows.

Calculation example When manufacturing particles for supporting organisms with a particle diameter of 31 and a ratio of 1 [0.75] by adding sand with a specific gravity of 26 to expanded polystyrene particles with a ratio of +1 (Oo2), from equation (8), 11 - (]204 /r- Therefore, choose sand with a grain size of α4, and
It turns out that it is only necessary to fill it with sparse density.

1. The particles for supporting organisms in this study are used for water treatment (
1. Each type of wafer is also used for the fermentation production of bamboo.

Microorganisms of the present invention
Since the resin is concentrated only on the surface of the synthetic resin particles, the formation of closed cells due to the crosslinking phenomenon is not possible. In addition, since only the synthetic resin is foamed in advance, any foaming ratio can be adopted.
By reducing the amount of synthetic resin used, the particle cost can be reduced. Furthermore, inorganic materials are kneaded into conventional synthetic resin.
In the case of the particles, the surface area of the inorganic material is at most a few percent to a few percent in terms of area ratio, but according to the present invention, it can be made to be 20 to 90%. , not only increases the amount of deposited inorganic and organic substances, but also
The surface shape of the particles also differs. Therefore, the adhesion of microorganisms is very good.

Next, what about the present invention? ) shows an example of the use of microorganism-carrying particles. Usage Example 1 A comparative experiment was conducted on the strength of the microorganism-carrying particles using the following six yuzu types, including commercially available ones. . Incidentally, there are four types of grains Fkl editions 3 to 3 according to the present invention. Particle cup 1 is a commercially available particle, and part 2 is JP-A-56-89.
These are the particles described in No. 897.

Table 1/3: Dried microorganisms 1] J, Mochikawa Q's ratio d (using the aerobic processing bag fif6 shown in Figure 2)
~606 types of microorganisms 1! ■Particle sanitary items Kikinuma IQ
Compare] 7. That is, the device 5 is made of transparent polyvinyl chloride resin and has a diameter of 4B and an effective height of 20cm. Filled with 400 cc of particles, mixed glucose and sodium citrate as raw water in a ratio of 1:1 in each of the Wt containers, and mixed 1301) with II.
J 40 n of artificial organic wastewater adjusted to 100 ppm
It was supplied from above the distributor 4 through the supply horn 2 through the piping 1 under the condition of oc/j+r. In addition, activated sludge of 1i71 nk was added as a seed culture to each apparatus rt 3 at the start of the operation. The amount of oxygen necessary for an aerobic microbial reaction is supplied from the supply pipe 7, and the draft tube 6 is used to ensure that the l'l'P element is not dissolved and that there is no shortage of dissolved oxygen. The dissolved oxygen at the bottom of the reaction section was measured, and the air supply was adjusted to 17 J. The waste water in the faucet 3 was circulated between the draft tube 6 and the reaction section where the microorganism-carrying particles 5 were present. The treated water is discharged from the system through a discharge pipe 8.

Measure the thickness of the microbial film. This measurement! Therefore, 20 particles were taken out from each device, the thickness of the microbial film was measured by γ-photography, and the average value was obtained.

The results are shown in Figure 3.

As is clear from FIG. 3, (II) the surface of the foamed polypropylene particle plate 1 is very rough, so the adhesion of microorganisms is hardly wetted.
Hydrophilicity is increased by mixing trJ talc, and unevenness is formed on the surface due to foaming, so the sticking to sanitary objects is good, but it is inferior to particle conditions 3 to 6 of the present invention. There is.

Particle shop 5-6 V of the present invention, 3 microorganisms JO are extremely good. Although it cannot be said that these particles V,j are necessarily covered with inorganic and organic substances in a close-packed state, it is thought that the attached particles are suitable nests for the growth of alligator organisms, and as a result, microorganisms are It seems that the number of *M Lll has been further increased.

Usage example 2 Yeast 5accharorrryces cerevis
The particles of the present invention are used in fermentation to convert glucose-15-4 to ethanol using iae (2).
(Because of this, a similar experiment was conducted using Particle Kneeling 2.

Using a glass device 16 having the shape shown in FIG.
A method was adopted in which the internal liquid was circulated using a pump.

The experimental conditions are as follows.

lj'eod method: Hutch formula % (11 Glucose concentration: 1o% Temperature: 30°C for 1 hour (adjusted fermentation period = 41% to about 5%) Culture simplified containing 10% glucose 1.35t to equipment 13
and filled with about 500 cc of microorganism-carrying particles. Yeast was added to an initial concentration of 5,000 ml. The culture medium was circulated through piping 11 by a pump 12. Note that since the fermentation is carried out in batch mode, there is no continuous supply of fresh glucose. In the figure, 16t1 is an extraction pipe for generated gas. 16 The mother sticks to the particles I:1 or the medium;
However, most of the -C is present in the particles, so when fermented with ethanol, -Jllll becomes 'I'i'/
blasphemy, = 'r''Otsu [λru.

Yeast is added to particle 5 (ii+'Jmen) zoζ, and f'! is attached to the particle. Peel off % rζL 1-7,
Measure the degree of late growth IIJ per unit of photoreceptor volume 5
141/ lko. The experiment U1 was conducted three times for the same particle, and the average f11'l was determined.

The results are shown in Table 2-1'.

Part 2,/.

Particle Ai 2 Particle Bread 3 Run 1 2500IIP
580 flllllll12 5040
64nO3287060511 Average 2805 60B5 As is clear from Table 2, the branch of the present invention has the ability to stick to living things even as a 5-layer sanitary material carrier for fermentation. There is.

[Brief explanation of drawings]

FIG. 1 is a model of microorganism-supporting particles of the present invention, and A
1) Synthetic resin resin, B shows the stuck particles (N1'!) of some substances and 41 substances. , Fig. 3 is a graph showing the relationship between the first fermentation period and the first microbial film. ?″i', 2.12・1 pump, ro,
15... device i't, 4. 14. Self-distributor-95, 15...Particle'1. '1'K [Applicant Chiyo I (1 Chemical Engineering Ke1! Established Stock Company Ne I-Kon Patent Attorney Kubo III iM Department 1
9- Figure 1 Figure 2 Figure 4

Claims (1)

[Scope of Claims] 1) A foamed synthetic tree is formed on the surface of 6 particles of inorganic and organic substances having a diameter of s o /7 J and above L below 1/portion of the particle diameter. The specific gravity is 02 to 1, made by adding one or more substances selected from the following:
0 range of 61 literary creatures 111 holding particles. 2) Patent f where the foamed filtration 4li1 fat particles have a particle size of 1 to 50 square meters. :'f Particles possessing the microorganism 111 described in item 1 of the desired scope. 5) 1IlU machine Wf is sand, natural crushed stone, activated carbon,
Claim 1J which is pearlite, shirasu balloon, gypsum, alumina, zeolite and ceramics
'I' is a particle for supporting microorganisms. 4) Organic substances are Ansbuza 419 coal and coke. Pitch, waste/C on exchange resin and rubber patent: i
+'! Particles for supporting microorganisms according to item 1. 5) Combined l'+I/, s+ l1t-f personnel are polystyrene and polyethylene. Particles for supporting microorganisms according to the 1M box No. 1 of the patent application, which are obtained by extracting polypropylene and poly(11) from vinyl chloride.